Lambda Red recombinase-mediated integration of the high molecular weight DNA into the Escherichia coli chromosome

High molecular weight DNA assembly in vivo for synthetic biology applications

(2016) Mario Juhas et al.   CRITICAL REVIEWS IN BIOTECHNOLOGY

Integrative bacterial artificial chromosomes for DNA integration into the Bacillus subtilis chromosome

(2016) Mario Juhas et al.   JOURNAL OF MICROBIOLOGICAL METHODS

On the road to synthetic life: the minimal cell and genome-scale engineering

(2015) Mario Juhas   CRITICAL REVIEWS IN BIOTECHNOLOGY

Identification and Validation of Novel Chromosomal Integration and Expression Loci in Escherichia coli Flagellar Region 1

(2015) Mario Juhas et al.   PLoS One

Flagellar region 3b supports strong expression of integrated DNA and the highest chromosomal integration efficiency of theEscherichia coliflagellar regions

(2015) Mario Juhas et al.   Microbial Biotechnology

Bacillus subtilis and Escherichia coli essential genes and minimal cell factories after one decade of genome engineering

(2014) M. Juhas et al.   MICROBIOLOGY-SGM

Escherichia coli Flagellar Genes as Target Sites for Integration and Expression of Genetic Circuits

(2014) Mario Juhas et al.   PLoS One

Meeting report: The Cambridge BioDesign TechEvent - Synthetic Biology, a new “Age of Wonder”?

(2013) Mario Juhas et al.   Biotechnology Journal

Development of a Fur-dependent and tightly regulated expression system in Escherichia coli for toxic protein synthesis

(2013) Lingyu Guan et al.   BMC BIOTECHNOLOGY

Knock-in/Knock-out (KIKO) vectors for rapid integration of large DNA sequences, including whole metabolic pathways, onto the Escherichia coli chromosome at well-characterised loci

(2013) Suriana Sabri et al.   Microbial Cell Factories

One-Step Cloning and Chromosomal Integration of DNA

(2013) François St-Pierre et al.   ACS Synthetic Biology

A PCR-free cloning method for the targeted φ80 Int-mediated integration of any long DNA fragment, bracketed with meganuclease recognition sites, into the Escherichia coli chromosome

(2012) Anna A. Ublinskaya et al.   JOURNAL OF MICROBIOLOGICAL METHODS

Methods and applications for assembling large DNA constructs

(2012) Chuck Merryman et al.   METABOLIC ENGINEERING

The future of metabolic engineering and synthetic biology: Towards a systematic practice

(2012) Vikramaditya G. Yadav et al.   METABOLIC ENGINEERING

Genetically switched d-lactate production in Escherichia coli

(2012) Li Zhou et al.   METABOLIC ENGINEERING

The genome sequence of E. coli W (ATCC 9637): comparative genome analysis and an improved genome-scale reconstruction of E. coli

(2011) Colin T Archer et al.   BMC GENOMICS

Metabolic engineering of Escherichia coli for direct production of 1,4-butanediol

(2011) Harry Yim et al.   Nature Chemical Biology

Site-specific chromosomal integration of large synthetic constructs

(2010) Thomas E. Kuhlman et al.   NUCLEIC ACIDS RESEARCH

Direct and indirect effects of H-NS and Fis on global gene expression control in Escherichia coli

(2010) Christina Kahramanoglou et al.   NUCLEIC ACIDS RESEARCH

Isoprenoid Pathway Optimization for Taxol Precursor Overproduction in Escherichia coli

(2010) P. K. Ajikumar et al.   SCIENCE

Understanding plasmid effect on hyaluronic acid molecular weight produced by Streptococcus equi subsp. zooepidemicus

(2009) Esteban Marcellin et al.   METABOLIC ENGINEERING

Factors affecting plasmid production in Escherichia coli from a resource allocation standpoint

(2009) Drew S Cunningham et al.   Microbial Cell Factories

Protein Occupancy Landscape of a Bacterial Genome

(2009) Tiffany Vora et al.   MOLECULAR CELL

Enzymatic assembly of DNA molecules up to several hundred kilobases

(2009) Daniel G Gibson et al.   NATURE METHODS