Review
Chemistry, Analytical
Yingying Li, Yang Hu, Tao Chen, Yan Chen, Yi Li, Haibo Zhou, Danting Yang
Summary: This review discusses and summarizes advanced detection and sensing strategies for P. aeruginosa cells and its associated quorum sensing molecules over the past ten years. The aim is to achieve rapid, sensitive, and cost-effective diagnosis for infection. The principles of commonly used sensing strategies are briefly introduced, followed by the advancement of these techniques in detecting P. aeruginosa cells and quorum sensing biomarkers. Additionally, the integration of novel platforms for clinical application is highlighted. The current achievements are summarized, and challenges and prospects for the future are proposed.
Article
Biochemistry & Molecular Biology
Megha Shah, Veronique L. Taylor, Diane Bona, Yvonne Tsao, Sabrina Y. Stanley, Sheila M. Pimentel-Elardo, Matthew McCallum, Joseph Bondy-Denomy, P. Lynne Howell, Justin R. Nodwell, Alan R. Davidson, Trevor F. Moraes, Karen L. Maxwell
Summary: The study identified a new phage anti-activator protein, Aqs1, which inhibits phage infection by suppressing the quorum-sensing master regulator LasR. Aqs1 also inhibits the bacterial type IV pilus assembly ATPase protein PilB, preventing phages from superinfecting host cells. This research highlights the potential of small phage proteins to disrupt key biological pathways and offers insights into the evolutionary dynamics between phages and their hosts.
Review
Chemistry, Medicinal
Qiaoqiang Li, Shen Mao, Hong Wang, Xinyi Ye
Summary: The survival pressure caused by antibiotics prompts bacteria to develop drug resistance. Quorum sensing is an intercellular communication system that regulates bacterial virulence and biofilm formation. In Pseudomonas aeruginosa, the formation and diffusion of biofilm is closely related to the quorum sensing system. Quorum-sensing inhibitors can reduce bacterial toxicity and enhance sensitivity to antibiotics, making them a popular topic for research and development in anti-infection. This review provides an overview of the Pseudomonas aeruginosa quorum-sensing system and quorum-sensing inhibitors research, which will aid in the future development of novel inhibitors.
Review
Biochemistry & Molecular Biology
Kayeen Vadakkan, Ajit Kumar Ngangbam, Kuppusamy Sathishkumar, Nelson Pynadathu Rumjit, Meena Kochappan Cheruvathur
Summary: Pseudomonas aeruginosa, a competitive and biofilm organism with complex quorum systems, poses a significant threat to the medical industry. Inhibiting quorum sensing could be a potential strategy for treating bacterial infections and developing anti-virulent tools.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Multidisciplinary Sciences
Parker Smith, Martin Schuster
Summary: Quorum sensing is a widespread cell density-dependent signaling mechanism in bacteria. This study demonstrates the role of antiactivators in preventing self-sensing in the quorum sensing system of Pseudomonas aeruginosa.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Chemistry, Multidisciplinary
Alex Yashkin, Josep Rayo, Larson Grimm, Martin Welch, Michael M. Meijler
Summary: In recent years, there has been a troubling increase in antibiotic resistance among bacterial pathogens globally, highlighting the importance of understanding the mechanisms that pathogens use to survive in complex environments. The human pathogen Pseudomonas aeruginosa employs various small molecules to regulate quorum sensing, with N-butyryl-L-homoserine lactone playing a significant role in its virulence regulon. Through activity-based protein profiling, researchers have identified uncharacterized proteins in the P. aeruginosa QS network and uncovered a new role of this natural autoinducer in inhibiting pyocyanin production through its interaction with PhzB1/2.
Article
Microbiology
Pauline Nogaret, Fatima El Garah, Anne-Beatrice Blanc-Potard
Summary: A novel vertebrate animal model using zebrafish was developed to assess drug efficacy against P. aeruginosa, with validation using a clinically used antibiotic and a novel quorum sensing inhibitory molecule. This study provides a new and reliable method for comparing the virulence of P. aeruginosa strains in vivo and evaluating the efficacy of antipseudomonal drugs, including new antivirulence compounds.
Article
Microbiology
Shouyi Li, Xuetao Gong, Liwen Yin, Xiaolei Pan, Yongxin Jin, Fang Bai, Zhihui Cheng, Un-Hwan Ha, Weihui Wu
Summary: This study reveals a novel regulatory mechanism in Pseudomonas aeruginosa, where CspC protein regulates the quorum-sensing systems by repressing the translation of a negative regulator gene, rsaL, in response to a host-generated metabolite.
Article
Microbiology
Alejandro Vasquez-Rifo, Jamie Cook, Deborah L. McEwan, Dania Shikara, Frederick M. Ausubel, Francesca Di Cara, Zhenyu Cheng
Summary: This study systematically compares the virulence of wild-type P. aeruginosa and QS mutants in selected hosts and conditions. It contributes to the long-term goal of understanding the intricate roles of different branches of the P. aeruginosa QS network in different hosts, and serves as a valuable resource for the field of host-pathogen interactions.
Article
Infectious Diseases
Sahil Shandil, Tsz Tin Yu, Shekh Sabir, David StC. Black, Naresh Kumar
Summary: As bacteria develop resistance against antimicrobials, alternative methods to tackle this issue must be developed. In this study, quinazolinone inhibitors were designed using molecular docking to target the pqs system in P. aeruginosa. Compound 6b showed the highest pqs inhibitory activity and no bacterial growth inhibition.
Article
Infectious Diseases
Shimaa M. Ghanem, Rehab Mahmoud Abd El-Baky, Mohamed A. S. Abourehab, Gamal F. M. Fadl, Nancy G. F. M. Gamil
Summary: This study investigated the frequency of virulence genes and their association with antibiotic resistance in Pseudomonas aeruginosa infections. The results showed a high prevalence of multi-drug resistant isolates and a significant association between various virulence genes and multi-drug resistance. This highlights the importance of these genes in the progression of infections and poses a great challenge for healthcare teams.
INFECTION AND DRUG RESISTANCE
(2023)
Article
Biochemistry & Molecular Biology
Suqi Hao, Dan Yang, Ling Zhao, Fei Shi, Gang Ye, Hualin Fu, Juchun Lin, Hongrui Guo, Ran He, Jianlong Li, Hongwei Chen, Muhammad Faraz Khan, Yinglun Li, Huaqiao Tang
Summary: EGCG, a bioactive ingredient in green tea, demonstrated significant inhibitory effects on the production of virulence factors in Pseudomonas aeruginosa, potentially serving as a candidate for quorum sensing inhibition to combat bacterial infections.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Microbiology
Rakesh Sikdar, Mikael H. Elias
Summary: Quorum sensing (QS) is a cell-to-cell communication system that regulates bacterial behaviors. Enzymatic quorum quenching (QQ) can interfere with QS and potentially enhance the effects of antibiotics. This study evaluated the impact of QS signal disruption on antibiotic resistance in Pseudomonas aeruginosa and found that it significantly affects resistance. The results highlight the complex relationship between QS and antibiotic resistance and suggest a case-by-case approach to combination therapy.
MICROBIOLOGY SPECTRUM
(2022)
Article
Infectious Diseases
Basmah Almohaywi, Tsz Tin Yu, George Iskander, Shekh Sabir, Mohan Bhadbhade, David StC. Black, Naresh Kumar
Summary: The study found that a range of dihydropyrrolone analogues synthesized through chemical reactions can inhibit the quorum-sensing system of Pseudomonas aeruginosa, thereby controlling its pathogenicity and biofilm formation. The structure of the compounds was also found to significantly affect their inhibitory activity.
Article
Infectious Diseases
Ahmed Al Saqr, Mohammed F. Aldawsari, El-Sayed Khafagy, Moataz A. Shaldam, Wael A. H. Hegazy, Hisham A. Abbas
Summary: Pseudomonas aeruginosa can cause healthcare-associated infections through its virulence factors controlled by the quorum sensing system. Allopurinol, an FDA-approved drug, shows promising anti-virulence and anti-QS activities by competing with autoinducers and downregulating QS genes. It may be useful in future treatment of P. aeruginosa infections.
Review
Biochemistry & Molecular Biology
Kathrin S. Froehlich, Kai Papenfort
MOLECULAR MICROBIOLOGY
(2016)
Article
Biochemistry & Molecular Biology
Kathrin S. Froehlich, Katharina Haneke, Kai Papenfort, Joerg Vogel
NUCLEIC ACIDS RESEARCH
(2016)
Article
Biochemistry & Molecular Biology
Kathrin Sophie Froehlich, Kai Papenfort, Agnes Fekete, Joerg Vogel
Article
Biochemistry & Molecular Biology
Kathrin S. Froehlich, Kai Papenfort, Allison A. Berger, Joerg Vogel
NUCLEIC ACIDS RESEARCH
(2012)
Article
Biochemistry & Molecular Biology
Kathrin S. Froehlich, Konrad U. Foerstner, Zemer Gitai
NUCLEIC ACIDS RESEARCH
(2018)
Article
Biology
Daniela Dimastrogiovanni, Kathrin S. Foerhlich, Katarzyna J. Bandyra, Heather A. Bruce, Susann Hohensee, Joerg Vogel, Ben F. Luisi
Article
Biochemistry & Molecular Biology
Roman Herzog, Nikolai Peschek, Kathrin S. Froehlich, Kilian Schumacher, Kai Papenfort
NUCLEIC ACIDS RESEARCH
(2019)
Article
Multidisciplinary Sciences
Andrew Santiago-Frangos, Kathrin S. Froehlich, Jeliazko R. Jeliazkov, Ewelina M. Malecka, Giada Marino, Jeffrey J. Gray, Ben F. Luisi, Sarah A. Woodson, Steven W. Hardwick
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2019)
Article
Microbiology
Colleen M. Bianco, Kathrin S. Froehlich, Carin K. Vanderpool
JOURNAL OF BACTERIOLOGY
(2019)
Editorial Material
Biochemistry & Molecular Biology
Kathrin S. Froehlich, Kai Papenfort
MOLECULAR MICROBIOLOGY
(2020)
Article
Multidisciplinary Sciences
Michaela Huber, Kathrin S. Froehlich, Jessica Radmer, Kai Papenfort
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2020)
Article
Multidisciplinary Sciences
Nikolai Peschek, Roman Herzog, Praveen K. Singh, Marcel Sprenger, Fabian Meyer, Kathrin S. Froehlich, Luise Schroeger, Marc Bramkamp, Knut Drescher, Kai Papenfort
NATURE COMMUNICATIONS
(2020)
Review
Microbiology
Kathrin S. Froehlich, Manuel Velasco Gomariz
Summary: Binding proteins play a significant role in bacterial gene expression control, especially in Caulobacter where RNA chaperones and ribonucleases contribute to regulatory RNA activity. Riboswitches and RNA thermometers regulate downstream open reading frames, while small regulatory RNAs control targets through base-pairing interactions.
CURRENT OPINION IN MICROBIOLOGY
(2021)
Article
Microbiology
Kathrin S. Froehlich, Susan Gottesman
MICROBIOLOGY SPECTRUM
(2018)