Article
Microbiology
Rakesh Sikdar, Mikael H. Elias
Summary: Quorum sensing (QS) is a cell-to-cell communication system that regulates bacterial behaviors. Enzymatic quorum quenching (QQ) can interfere with QS and potentially enhance the effects of antibiotics. This study evaluated the impact of QS signal disruption on antibiotic resistance in Pseudomonas aeruginosa and found that it significantly affects resistance. The results highlight the complex relationship between QS and antibiotic resistance and suggest a case-by-case approach to combination therapy.
MICROBIOLOGY SPECTRUM
(2022)
Review
Chemistry, Medicinal
Qiaoqiang Li, Shen Mao, Hong Wang, Xinyi Ye
Summary: The survival pressure caused by antibiotics prompts bacteria to develop drug resistance. Quorum sensing is an intercellular communication system that regulates bacterial virulence and biofilm formation. In Pseudomonas aeruginosa, the formation and diffusion of biofilm is closely related to the quorum sensing system. Quorum-sensing inhibitors can reduce bacterial toxicity and enhance sensitivity to antibiotics, making them a popular topic for research and development in anti-infection. This review provides an overview of the Pseudomonas aeruginosa quorum-sensing system and quorum-sensing inhibitors research, which will aid in the future development of novel inhibitors.
Article
Infectious Diseases
Wen-Jie Ng, Chin-Lu Hing, Choon-Boq Loo, Ee-Khang Hoh, Ian-Lung Loke, Kah-Yaw Ee
Summary: The main objective of this study was to determine the impacts of honey and ginger-enriched honey on the QS virulence factors and biofilm formation of antibiotic resistant P. aeruginosa clinical isolates. Outcomes showed honey and/or ginger-enriched honey significantly reduced the protease activity, pyocyanin production and exotoxin A concentration of the isolates. The swarming and swimming motility together with biofilm formation in all clinical isolates were also significantly inhibited by both honey samples.
Article
Biochemistry & Molecular Biology
Rafael Amieva, Teresa Gil-Gil, Jose Luis Martinez, Manuel Alcalde-Rico
Summary: Multidrug efflux pumps are crucial for antibiotic resistance in bacteria, but they also modulate other aspects of bacterial physiology, such as quorum sensing and iron homeostasis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Suqi Hao, Dan Yang, Ling Zhao, Fei Shi, Gang Ye, Hualin Fu, Juchun Lin, Hongrui Guo, Ran He, Jianlong Li, Hongwei Chen, Muhammad Faraz Khan, Yinglun Li, Huaqiao Tang
Summary: EGCG, a bioactive ingredient in green tea, demonstrated significant inhibitory effects on the production of virulence factors in Pseudomonas aeruginosa, potentially serving as a candidate for quorum sensing inhibition to combat bacterial infections.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Infectious Diseases
Ahmed Al Saqr, Mohammed F. Aldawsari, El-Sayed Khafagy, Moataz A. Shaldam, Wael A. H. Hegazy, Hisham A. Abbas
Summary: Pseudomonas aeruginosa can cause healthcare-associated infections through its virulence factors controlled by the quorum sensing system. Allopurinol, an FDA-approved drug, shows promising anti-virulence and anti-QS activities by competing with autoinducers and downregulating QS genes. It may be useful in future treatment of P. aeruginosa infections.
Article
Immunology
Osama Nassar, Said E. Desouky, Gamal M. El-Sherbiny, Mohammed Abu-Elghait
Summary: This study evaluated the virulence traits of Pseudomonas aeruginosa clinical isolates and found significant correlations between biofilm formation, swimming and swarming motility, and pyocyanin production with other studied virulence traits. However, no significant correlation was found between these traits and protease activity. Additionally, a significant negative correlation was found between these traits and antibiotic susceptibility.
MICROBIAL PATHOGENESIS
(2022)
Article
Microbiology
Wen-Ru Li, Tao-Hua Zeng, Zhi-Qing Zhang, Qing-Shan Shi, Xiao-Bao Xie
Summary: In this study, it was found that geraniol can inhibit the growth of Pseudomonas aeruginosa, prolong the lag phase and growth period. Geraniol also suppresses the expression of the three quorum sensing systems of P. aeruginosa, thereby attenuating the virulence factors regulated by these systems. This research is significant for improving the treatment of bacterial infections caused by P. aeruginosa.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Microbiology
Dan Yang, Suqi Hao, Ling Zhao, Fei Shi, Gang Ye, Yuanfeng Zou, Xu Song, Lixia Li, Zhongqiong Yin, Xiaoli He, Shiling Feng, Helin Chen, Yu Zhang, Yuanze Gao, Yinglun Li, Huaqiao Tang
Summary: The study found that paeonol has good anti-biofilm activity against Gram-negative bacteria and anti-QS activity, effectively reducing the virulence of P. aeruginosa. In vivo studies showed that paeonol could reduce the pathogenicity of P. aeruginosa and enhance the survival rate of C. elegans.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Infectious Diseases
Tomasz Bogiel, Malgorzata Prazynska, Joanna Kwiecinska-Pirog, Agnieszka Mikucka, Eugenia Gospodarek-Komkowska
Summary: The study compared the frequency of selected enzymatic virulence factor genes among non-duplicated carbapenem-resistant P. aeruginosa isolates, finding a higher prevalence of alkaline protease gene and a 37.4% detection rate for neuraminidase-1 gene. Significant differences in the prevalence of lasB and nan1 were observed between MBL-positive and MBL-negative strains.
Review
Microbiology
Kayla A. Simanek, Jon E. Paczkowski
Summary: Bacteria use a process called quorum sensing to coordinate collective behaviors. This process relies on the detection of extracellular signal molecules known as autoinducers. In the human pathogen Pseudomonas aeruginosa, quorum sensing is important for virulence and biofilm formation. However, the signaling hierarchy and regulatory mechanisms of the receptors involved in this process are still not well understood, leading to chronic infection.
Article
Medicine, Research & Experimental
Ya Fei Geng, Cheng Yang, Yi Zhang, Sheng Nan Tao, Jie Mei, Xu Chang Zhang, Ya Juan Sun, Bing Tian Zhao
Summary: This study investigated the potential of luteolin as a quorum sensing inhibitor against Pseudomonas aeruginosa, demonstrating its effectiveness in inhibiting biofilm formation, virulence factor production, and bacterial motility. Luteolin was also found to reduce the accumulation of QS signaling molecules and downregulate the transcription of QS genes, suggesting its promising role in combating drug-resistant infections caused by P. aeruginosa.
Article
Microbiology
Maan T. Khayat, Tarek S. Ibrahim, Khaled M. Darwish, Ahdab N. Khayyat, Majed Alharbi, El-Sayed Khafagy, Mohamed A. M. Ali, Wael A. H. Hegazy, Hisham A. Abbas
Summary: Bacteria communicate through quorum sensing, and Pseudomonas aeruginosa is a clinically important pathogen with high antibiotic resistance and controlled virulence factors through quorum sensing. This study evaluates the anti-quorum sensing and anti-virulence activities of linagliptin against P. aeruginosa, and finds that it effectively inhibits virulence factor production and reduces pathogenesis.
Article
Food Science & Technology
Seyed Majid Hashemi Dehkordi, Seyed Amirali Anvar, Ebrahim Rahimi, Hamed Ahari, Maryam Ataee
Summary: This study aimed to investigate the prevalence of Pseudomonas aeruginosa, antibiotic resistance, and virulence factor encoding genes in lobster samples. The results showed that bacterial contamination in the samples was not directly related to the cooler climate in summer. The analysis of antibiotic resistance, antibiotic resistance encoding genes, and Multi-Drug Resistance (MDR) patterns highlighted the importance of maintaining the cold chain in the storage, transportation, and distribution of marine samples.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2022)
Review
Biotechnology & Applied Microbiology
Qingying Fan, Jing Zuo, Haikun Wang, Daniel Grenier, Li Yi, Yang Wang
Summary: Quorum sensing (QS) is an important part of cell/cell communication, especially in bacteria. Bacteria use QS to perceive and regulate gene expression to adapt to their complex habitats, including controlling the expression of virulence factors and antibiotic resistance. Targeting QS has proven to be a promising alternative strategy for controlling infections, especially in zoonotic bacteria.
BIOTECHNOLOGY ADVANCES
(2022)
Review
Pharmacology & Pharmacy
Afsana Sheikh, Mohammed A. S. Abourehab, Prashant Kesharwani
Summary: 4D printing, which involves the fourth dimension of 'time', allows programmed objects to change shape in response to external stimuli. This technique has promising applications in cancer treatment, drug delivery, stent development, and tissue engineering. This review focuses on the development of 4D-printed objects, their clinical use, and the potential of 5D printing to revolutionize biomedical engineering and drug delivery.
DRUG DISCOVERY TODAY
(2023)
Review
Pharmacology & Pharmacy
Xiaoling Fu, Urushi Rehman, Liuya Wei, Zhe-Sheng Chen, Mohammed A. S. Abourehab, Prashant Kesharwani, Zhi-Hong Cheng
Summary: To develop next-generation nanomedicine, theranostic nanotherapeutic strategies are increasingly being emphasized. In recent years, the effective lifetime of anti-bacterial and anti-cancer agent is diminishing, which undermines the economic incentives necessary for clinical development and therapeutic applications. This review provides an informative overview regarding the numerous aspects of bactericidal and other biomedical applications of Ag-dendrimer nanocomposites, particularly emphasizing analysis of existing research and prospective worth to the pharmaceutical sector in future.
DRUG RESISTANCE UPDATES
(2023)
Article
Polymer Science
Urushi Rehman, Mohammed A. S. Abourehab, Amit Alexander, Prashant Kesharwani
Summary: Pancreatic cancer (PC) is a deadly disease with a low 5-year survival rate and is expected to become the second leading cause of cancer-related deaths in the United States by 2030. Treatment options are limited and the tumor microenvironment hinders therapeutic agents. Combining chemotherapeutics using nanoparticle-mediated delivery, such as micelles, shows promise in improving treatment outcomes and reducing side effects.
EUROPEAN POLYMER JOURNAL
(2023)
Article
Biochemistry & Molecular Biology
Adel Al Fatease, Mai E. E. Shoman, Mohammed A. S. Abourehab, Heba A. A. Abou-Taleb, Hamdy Abdelkader
Summary: In this study, L-arginine was used to form a salt with curcumin, aiming to enhance its solubility and anticancer activities. Two preparation methods, physical mixing and coprecipitation, were adopted. The formed curcumin L-arginine salt showed significantly improved solubility and cytotoxicity, comparable to the standard anticancer drug Taxol.
Article
Biochemistry & Molecular Biology
Heba H. A. Hassan, Muhammad I. Ismail, Mohammed A. S. Abourehab, Frank M. Boeckler, Tamer M. Ibrahim, Reem K. Arafa
Summary: This study built an active compound set and evaluated four docking programs to find new Fascin binding site blockers. PLANTS showed the best screening performance and identified potential binders to Fascin binding site 2. Molecular dynamics simulations confirmed Remdesivir and NANPDB3 as potential binders. This study provides a model for virtual screening of new potential targets for cancer therapies.
Article
Chemistry, Physical
Mohanad Bashari, Hani Ahmed, Ayman Balla Mustafa, Asad Riaz, Jinpeng Wang, Salina Yahya Saddick, Abdulkader Shaikh Omar, Mohamed Afifi, Ammar Al-Farga, Lulwah Zeyad AlJumaiah, Mohammed A. S. Abourehab, Amany Belal, Mohamed Y. Zaky
Summary: This study investigated a novel method involving ultrasound and microwave shock (UMS) to enhance the nano-entrapment of enzymes in a Ca-alginate gel. The effects of UMS treatment on the loading effectiveness (LE) and immobilization yield (IY) of dextranase enzymes were examined. The microstructure of the immobilized enzyme (IE) was characterized using FT-IR spectra and SEM. Compared to the control, UMS treatment significantly improved the reusability and enzyme-kinetics characteristics of the IE, resulting in increased LE and IY by 97.32% and 78.25%, respectively.
Article
Biochemistry & Molecular Biology
Ahmed Elkamhawy, Na Kyoung Oh, Noha A. A. Gouda, Magda H. H. Abdellattif, Saud O. O. Alshammari, Mohammed A. S. Abourehab, Qamar A. A. Alshammari, Amany Belal, Minkyoung Kim, Ahmed A. A. Al-Karmalawy, Kyeong Lee
Summary: A series of indole-based caffeic acid amide analogues were synthesized and evaluated for their antioxidant activity. Compound 3j exhibited the highest antioxidant activity and could be a potential drug for the treatment of oxidative stress-related diseases.
Article
Chemistry, Medicinal
Naheed Akhter, Sidra Batool, Samreen Gul Khan, Nasir Rasool, Fozia Anjum, Azhar Rasul, Sevki Adem, Sadaf Mahmood, Aziz Ur Rehman, Mehr Un Nisa, Zainib Razzaq, Jorn B. Christensen, Mohammed A. S. Abourehab, Syed Adnan Ali Shah, Syahrul Imran
Summary: In this study, triazole-based acetamides were synthesized by chemically modifying 2-(4-isobutylphenyl) propanoic acid. The target compounds were produced in good yields and their structures were confirmed by various physiochemical and spectroscopic methods. The anti-liver carcinoma effects of these compounds were investigated and compound 7f showed the highest anti-proliferative activity with low toxicity. Molecular docking revealed that compound 7f exhibited excellent binding affinities to c-kit tyrosine kinase and protein kinase B. Therefore, compound 7f is considered as a suitable drug candidate for the treatment of hepatocellular carcinoma.
Article
Biophysics
Neha Parveen, Mohammed A. S. Abourehab, Punniyakoti Veeraveedu Thanikachalam, Roop K. Khar, Prashant Kesharwani
Summary: Skin conditions have a significant impact on individuals and society. The barrier mechanism of the skin and physicochemical characteristics of drugs often limit drug penetration, leading to the need for innovative drug delivery methods. Nanocrystals have been investigated as a method to enhance skin penetration and this review explores the barriers of skin penetration, modern methods for topical distribution enhancement, and the use of nanocrystals to overcome these barriers. Researchers working on challenging topical drug delivery may find this research relevant.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2023)
Review
Biochemistry & Molecular Biology
Punniyakoti Veeraveedu Thanikachalam, Srinivasan Ramamurthy, Poojitha Mallapu, Sudhir Rama Varma, Jayaraj Narayanan, Mohammed A. S. Abourehab, Prashant Kesharwani
Summary: IL-33 is a cytokine that belongs to the IL-1 family and promotes the production of Th2 cytokines by binding with ST2L and IL-1RAcP. It has demonstrated protective effects against cardiovascular diseases by inducing Th2 cytokines and promoting alternative activating M2 polarization. However, the soluble decoy form of ST2 mitigates its effects, exacerbating cardiovascular diseases. IL-33 also plays a significant role in the development of asthma, arthritis, atopic dermatitis, and anaphylaxis. This review aims to explore the protective role of IL-33 against cardiovascular diseases and the potential of serum soluble ST2 as a diagnostic biomarker for these diseases. Therefore, IL-33 holds promise as a potential therapeutic target for cardiovascular diseases.
CYTOKINE & GROWTH FACTOR REVIEWS
(2023)
Article
Genetics & Heredity
Magda Assem, Rady E. E. El-Araby, Ahmed A. A. Al-Karmalawy, Reem Nabil, Mohamed A. M. Kamal, Amany Belal, Heba I. I. Ghamry, Mohammed A. S. Abourehab, Mohammed M. M. Ghoneim, Mohammad Y. Y. Alshahrani, Asmaa A. A. El Leithy
Summary: In this study, we investigated the methylation levels and expression of Galectin-3 and Galectin-12 in primary leukemic cells from patients with AML. We found a significant correlation between promoter methylation and loss of LGALS12 gene expression in AML patients. Moreover, we identified specific CpG sites in the promoter region of galectin-12 that must be unmethylated for gene expression to occur.
FRONTIERS IN GENETICS
(2023)
Article
Environmental Sciences
Sahim Aziz Hazari, Afsana Sheikh, Mohammed A. S. Abourehab, Alaa S. Tulbah, Prashant Kesharwani
Summary: Increased thickness of the skin and hyperproliferation of keratinocyte cell make the treatment of psoriasis difficult. Gallic Acid (GA) has proven effective against keratinocyte cell hyperproliferation while lipid-polymer loaded hybrid nanoparticles (LPHNs) have advantages in drug loading, controlled release, stability, and retention. The optimized LPHNs demonstrated enhanced drug penetration and release, leading to reduced PASI score and splenomegaly without causing serious irritation. The prepared formulation controlled the disease effectively with normal immune cell levels, making GALPHN gel a promising treatment for psoriasis.
ENVIRONMENTAL RESEARCH
(2023)
Article
Chemistry, Medicinal
Sara Mahmoud Farhan, Rehab Mahmoud Abd El-Baky, Hala Rady Ahmed, Zeinab Fathalla, Ali Alamri, Hamdy Abdelkader, Adel Al Fatease
Summary: Extensively drug-resistant, multidrug-resistant, and pandrug-resistant Gram-negative microorganisms are a significant global threat. The combination of beta-lactam and aminoglycoside drugs, as well as imipenem:cyclodextrin inclusion complexes, were studied for the treatment of lethal Gram-negative bacteria. The results showed that imipenem:cyclodextrin complexes had a significant reduction in bacterial count.
Article
Biotechnology & Applied Microbiology
Safy Hadiya, Reham A. Ibrahem, Rehab M. Abd El-Baky, Mahmoud Elsabahy, Abeer M. R. Hussein, Mohammed E. M. Tolba, Sherine A. Aly
Summary: This study demonstrates that loading ciprofloxacin and meropenem into nanocarriers can enhance their synergistic effect, providing a promising approach to rejuvenate antibiotics ineffective against resistant pathogens. Nanocarrier-loaded antibiotics exhibited a bactericidal effect and significantly reduced bacterial load, indicating their potential in treating bacterial infections.
