Review
Endocrinology & Metabolism
Diego Balboa, Diepiriye G. Iworima, Timothy J. Kieffer
Summary: The review discusses the recent progress in generating and characterizing functional stem cell-derived beta cells, as well as the possibilities and challenges in diabetes disease modeling using stem cells.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Article
Plant Sciences
Wen Yu, Yannan Wang, Di Jiang, Jie Shang, Miao Liu, Thomas Efferth, Chun-Bo Teng
Summary: This study discovered a novel strategy to induce pancreatic ductal progenitors to differentiate into insulin-producing cells using isoastragaloside I. This approach has the potential to be applied in islet transplantation for diabetes therapies.
Review
Pharmacology & Pharmacy
Mozafar Khazaei, Fatemeh Khazaei, Elham Niromand, Elham Ghanbari
Summary: Tissue engineering offers a promising approach for improving the treatment of type 1 diabetes by utilizing mesenchymal stem cells (MSCs) to differentiate into insulin-producing cells (IPCs). The use of scaffolds can enhance the differentiation of MSCs into IPCs, improving their viability and insulin production.
JOURNAL OF DRUG TARGETING
(2023)
Review
Medical Laboratory Technology
Emma S. Heaton, S. H. A. Jin
Summary: Scientists have developed the bi-hormonal abnormality hypothesis, stating that diabetes is not solely caused by impaired insulin signaling but also by inappropriate levels of glucagon. Traditional treatments for type 1 diabetes rely on insulin injections, disregarding the role of other endocrine cell types. Therefore, there is a need to develop pancreatic islet organoids that include all cell types to better replace the functionality of native islets.
TRANSLATIONAL RESEARCH
(2022)
Article
Multidisciplinary Sciences
Amanda M. Anderson, Laurie G. Landry, Aimon A. Alkanani, Laura Pyle, Alvin C. Powers, Mark A. Atkinson, Clayton E. Mathews, Bart O. Roep, Aaron W. Michels, Maki Nakayama
Summary: This study demonstrated that CD8 T cells targeting preproinsulin are more frequent in T1D patients, especially those with the HLA-A*02:01 allele, and show potent autoreactivity. These findings have implications for designing antigen-specific immunotherapies and identifying individuals who may benefit from such interventions.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Review
Cell & Tissue Engineering
Hilary E. Murray, Ali Zafar, Khalid M. Qureshi, Michelle B. Paget, Clifford J. Bailey, Richard Downing
Summary: Pancreatic islet cell transplantation is effective for treating type 1 diabetes, but is limited by donor organ availability and transplant procedure factors. Human amniotic epithelial cells show potential for improving outcomes by protecting islet function.
JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE
(2021)
Article
Cell & Tissue Engineering
Yu Na Lee, Hye-Jin Yi, Eun Hye Seo, Jooyun Oh, Song Lee, Sarah Ferber, Teruo Okano, In Kyong Shim, Song Cheol Kim
Summary: In this study, insulin-producing cells were trans-differentiated from adult human liver cells and formed into cell sheets, which showed significantly increased differentiation efficiency and engraftment efficacy compared to injected cells. The cell sheet technique not only improved blood glucose levels and weight gain, but also showed lower liver toxicity, indicating its potential for clinical applications with various cell sources.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Endocrinology & Metabolism
Pia Montanucci, Teresa Pescara, Alessia Greco, Giulia Leonardi, Luigi Marini, Giuseppe Basta, Riccardo Calafiore
Summary: The study found that hUCMS inhibited proliferation of allogeneic polymorphonuclear blood cells from patients with T1D, while promoting expansion of FoxP3(+)Tregs. Through co-microencapsulation, hUCMS and hIDC synergized anatomically and functionally, maintaining tracer insulin output by hIDC and utilizing the immunoregulatory properties of hUCMS to help decrease blood glucose levels.
DIABETES-METABOLISM RESEARCH AND REVIEWS
(2021)
Article
Cell Biology
Xi Wang, Kristina G. Maxwell, Kai Wang, Daniel T. Bowers, James A. Flanders, Wanjun Liu, Long-Hai Wang, Qingsheng Liu, Chengyang Liu, Ali Naji, Yong Wang, Bo Wang, Jing Chen, Alexander U. Ernst, Juan M. Melero-Martin, Jeffrey R. Millman, Minglin Ma
Summary: The study introduces a novel device composed of nanofibers for transplanting stem cell-derived beta cells to treat T1D, demonstrating its safety, efficacy, and sustainability in animal experiments.
SCIENCE TRANSLATIONAL MEDICINE
(2021)
Article
Gastroenterology & Hepatology
Mugdha Joglekar, Subhshri Sahu, Wilson K. M. Wong, Sarang N. Satoor, Charlotte X. Dong, Ryan J. Farr, Michael D. Williams, Prapti Pandya, Gaurang Jhala, Sundy N. Y. Yang, Yi Vee Chew, Nicola Hetherington, Dhan Thiruchevlam, Sasikala Mitnala, Guduru Rao, Duvvuru Nageshwar Reddy, Thomas Loudovaris, Wayne J. Hawthorne, Andrew G. Elefanty, Vinay M. Joglekar, Edouard G. Stanley, David Martin, Helen E. Thomas, David Tosh, Louise T. Dalgaard, Anandwardhan A. Hardikar
Summary: This study reveals the presence of functional insulin-producing cells in the epithelial cells of the gallbladder, both during development and adulthood. Interestingly, these insulin-producing cells in the gallbladder are not targeted by autoimmune cells in a mouse model of type 1 diabetes. These findings highlight the evolutionary and developmental similarities between the gallbladder and the pancreas, and provide potential insights for type 1 diabetes therapy.
CELLULAR AND MOLECULAR GASTROENTEROLOGY AND HEPATOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Musa T. Abidov, Ksenia Sokolova, Irina F. Gette, Irina G. Danilova
Summary: Beta-cell dysfunction plays a crucial role in the development of type 2 diabetes. The generation of extra-islet insulin-producing cells (IPCs) in the pancreas may serve as a compensatory mechanism. Inflammatory levels and Pdx1 expression are associated with the generation of extra-islet IPCs, and macrophages play a key role in diabetes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Medicine, Research & Experimental
Butian Wei, Xin Zhang, Jiwei Qian, Zhe Tang, Bo Zhang
Summary: Nrf2 is an important intracellular regulator of antioxidant stress, regulating not only antioxidant function but also insulin secretion, proliferation, and differentiation of beta cells, ER stress, and mitochondrial function. Pharmacological activation of Nrf2 has been shown to protect islet cells during different stages of transplantation in experiments.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Article
Immunology
Burcak Yesildag, Joan Mir-Coll, Aparna Neelakandhan, Claire B. Gibson, Nikole R. Perdue, Chantal Rufer, Maria Karsai, Adelinn Biernath, Felix Forschler, Patricia Wu Jin, Patrick M. Misun, Alexandra Title, Andreas Hierlemann, Frederik F. Kreiner, Johnna D. Wesley, Matthias G. von Herrath
Summary: In this study, three in-vitro islet-immune injury models were established to investigate the immunopathogenesis of type 1 diabetes. The results showed that liraglutide protected pancreatic cells from immune attack.
CLINICAL IMMUNOLOGY
(2022)
Article
Multidisciplinary Sciences
Xi Wang, Kai Wang, Ming Yu, Diana Velluto, Xuechong Hong, Bo Wang, Alan Chiu, Juan M. Melero-Martin, Alice A. Tomei, Minglin Ma
Summary: Engineered MSCs can improve the outcome of islet transplantation by reducing systemic immunosuppression and promoting immunomodulation. Immunophenotyping revealed reduced infiltration of T effector cells and increased infiltration of T regulatory cells in the co-transplanted islets with MSCs.
Article
Biotechnology & Applied Microbiology
Johanna F. Dekkers, Maria Alieva, Astrid Cleven, Farid Keramati, Amber K. L. Wezenaar, Esmee J. van Vliet, Jens Puschhof, Peter Brazda, Inez Johanna, Angelo D. Meringa, Heggert G. Rebel, Maj-Britt Buchholz, Mario Barrera Roman, Amber L. Zeeman, Sam de Blank, Domenico Fasci, Maarten H. Geurts, Annelisa M. Cornel, Else Driehuis, Rosemary Millen, Trudy Straetemans, Mara J. T. Nicolasen, Tineke Aarts-Riemens, Hendrikus C. R. Ariese, Hannah R. Johnson, Ravian L. van Ineveld, Froso Karaiskaki, Oded Kopper, Yotam E. Bar-Ephraim, Kai Kretzschmar, Alexander M. M. Eggermont, Stefan Nierkens, Ellen J. Wehrens, Henk G. Stunnenberg, Hans Clevers, Jurgen Kuball, Zsolt Sebestyen, Anne C. Rios
Summary: This study utilizes a system called BEHAV3D to investigate the dynamic interactions between immune cells and patient cancer organoids. By live-tracking a large number of engineered T cells cultured with patient-derived solid-tumor organoids, a cluster of "super engager" T cells with potent serial killing capacity is identified. The study also examines cancer metabolome-sensing engineered T cells (TEGs) and identifies behavior-specific gene signatures in these cells. Additionally, it demonstrates the ability of type I interferon to prime resistant organoids for TEG-mediated killing.
NATURE BIOTECHNOLOGY
(2022)
Article
Nursing
Michela Barisone, Luca Ghirotto, Erica Busca, Matias Eduardo Diaz Crescitelli, Monica Casalino, Giovanni Chilin, Simona Milani, Paola Sanvito, Barbara Suardi, Antonia Follenzi, Alberto Dal Molin
Summary: This study explores the clinical placement experiences of nursing students during the Covid-19 pandemic. The findings show that the pandemic provided learning opportunities for nursing students and allowed them to develop new skills. The study also highlights the importance of dignity in patient care and the role of nursing students as advocates.
NURSE EDUCATION IN PRACTICE
(2022)
Article
Genetics & Heredity
Antonella Fanelli, Silvia Vannelli, Deepak Babu, Simona Mellone, Alessia Cucci, Alice Monzani, Wael Al Essa, Andrea Secco, Antonia Follenzi, Simonetta Bellone, Flavia Prodam, Mara Giordano
Summary: Identified two small deletions in SHOX-haploinsufficiency patients, located in previously unknown regulatory element regions near SHOX, which expands the spectrum of variants contributing to SHOX-haploinsufficiency.
MOLECULAR GENETICS & GENOMIC MEDICINE
(2022)
Article
Cell Biology
Blair K. Gage, Simone Merlin, Cristina Olgasi, Antonia Follenzi, Gordon M. Keller
Summary: This study develops an efficient method to generate human pluripotent stem cell-derived liver sinusoidal endothelial cell (LSEC) progenitors from specific mesoderm sub-populations. The study shows that venous endothelial cells generated from certain mesoderm sub-populations are more efficient in LSEC engraftment and can secrete bioactive coagulation factor VIII. These findings highlight the importance of mesoderm induction in generating functional LSECs.
Correction
Cell Biology
Blair K. Gage, Simone Merlin, Cristina Olgasi, Antonia Follenzi, Gordon M. Keller
Summary: This study developed an efficient and scalable method to generate human pluripotent stem cell-derived liver sinusoidal endothelial cells (LSECs) progenitors from different mesoderm subpopulations, and found that appropriate mesoderm induction is crucial for generating functional LSECs.
Review
Medicine, General & Internal
Thierry Berney, Charles H. Wassmer, Fanny Lebreton, Kevin Bellofatto, Laura Mar Fonseca, Juliette Bignard, Reine Hanna, Andrea Peloso, Ekaterine Berishvili
Summary: Type 1 diabetes is caused by autoimmune destruction of insulin-producing beta cells in the pancreas. Pancreas or islet transplantation is the best therapeutic approach for severe complications, but faces challenges such as suboptimal engraftment and rejection. A bioartificial pancreas is a potential solution that can address these issues and prevent immune rejection and recurrence of autoimmunity.
Article
Oncology
Sander Ubels, Eric Matthee, Moniek Verstegen, Bastiaan Klarenbeek, Stefan Bouwense, Mark I. van Berge Henegouwen, Freek Daams, Jan Willem T. Dekker, Marc J. van Det, Stijn van Esser, Ewen A. Griffiths, Jan Willem Haveman, Grard Nieuwenhuijzen, Peter D. Siersema, Bas Wijnhoven, Gerjon Hannink, Frans van Workum, Camiel Rosman, Joos Heisterkamp, Fatih Polat, Jeroen Schouten, Pritam Singh
Summary: This study aimed to assess the differences in failure to rescue (FTR) after anastomotic leak (AL) between different centers. The results showed that high-volume centers had lower FTR, which could be explained by lower leak severity, lower secondary ICU readmission rate, and higher availability of therapeutic modalities.
Article
Surgery
Charles-Henri Wassmer, Rebecca Revol, Isabelle Uhe, Mickael Chevallay, Christian Toso, Pascal Gervaz, Philippe Morel, Pierre-Alexandre Poletti, Alexandra Platon, Frederic Ris, Frank Schwenter, Thomas Perneger, Raphael P. H. Meier
Summary: A practical clinical severity score was developed and validated to guide the management of patients with small bowel obstruction.
INTERNATIONAL JOURNAL OF SURGERY
(2023)
Article
Biochemical Research Methods
Ana Salome Pires, Sveva Bollini, Maria Filomena Botelho, Ingrid Lang-Olip, Peter Ponsaerts, Carolina Balbi, Anna Lange-Consiglio, Mathilde Fenelon, Slavko Mojsilovic, Ekaterine Berishvili, Fausto Cremonesi, Maria Gazouli, Diana Bugarski, Alexandra Gellhaus, Halima Kerdjoudj, Andreina Schoeberlein
Summary: Over the past 18 years, there has been a growing interest in the therapeutic use of perinatal derivatives (PnD). The COST SPRINT Action (CA17116) aims to provide systematic reviews of preclinical studies to understand the therapeutic potential and mechanisms of PnD in diseases and injuries. They describe strategies used to collect and analyze published data for meta-analyses and reviews of PnD therapies, with the goal of establishing standards for assessing safety and clinical benefit.
METHODS AND PROTOCOLS
(2023)
Article
Materials Science, Biomaterials
Raquel Fernandez-Penas, Cristobal Verdugo-Escamilla, Carla Triunfo, Stefanie Gaertner, Annarita D'Urso, Francesca Oltolina, Antonia Follenzi, Gabriele Maoloni, Helmut Coelfen, Giuseppe Falini, Jaime Gomez-Morales
Summary: We have developed a simple, one-step, low-temperature hydrothermal method to convert oyster shell waste particles into hydroxyapatite micro/nanoparticles. The transformation process was influenced by the choice of P reagents, P/bCCP molar ratios, digestion temperatures, and digestion times. The transformation occurred through a dissolution-reprecipitation mechanism, and both the original particles and the derived hydroxyapatite particles showed cytocompatibility and promoted osteogenic differentiation in stem cells.
JOURNAL OF MATERIALS CHEMISTRY B
(2023)
Article
Surgery
Thierry Berney, Axel Andres, Melena D. Bellin, Eelco J. P. de Koning, Paul R. V. Johnson, Thomas W. H. Kay, Torbjoern Lundgren, Michael R. Rickels, Hanne Scholz, Peter G. Stock, Steve White
Summary: A global online survey was conducted on 69 islet transplantation programs, revealing that a total of 4,365 islet allotransplants were performed in the 2000-2020 period, with notable differences in transplant types between North America and the rest of the world. The absence of health care coverage and the COVID-19 pandemic in 2020 have significantly hindered transplant activity.
TRANSPLANT INTERNATIONAL
(2022)
Article
Surgery
Charles-Henri Wassmer, Fanny Lebreton, Kevin Bellofatto, Lisa Perez, David Cottet-Dumoulin, Axel Andres, Domenico Bosco, Thierry Berney, Veronique Othenin-Girard, Begona Martinez De Tejada, Marie Cohen, Christina Olgasi, Antonia Follenzi, Ekaterine Berishvili
Summary: Lack of rapid revascularization and inflammatory attacks contribute to poor outcomes after clinical islet transplantation. In a study using pre-vascularized islet organoids, enhanced in vitro function, better engraftment, and improved vascularization were observed, mainly due to cross-talk between different cell types and upregulation of genes promoting angiogenesis and beta cell function.
TRANSPLANT INTERNATIONAL
(2022)
Article
Surgery
Sander Ubels, Moniek Verstegen, Bastiaan Klarenbeek, Stefan Bouwense, Mark van Berge Henegouwen, Freek Daams, Marc J. van Det, Ewen A. Griffiths, Jan W. Haveman, Joos Heisterkamp, Renol Koshy, Grard Nieuwenhuijzen, Fatih Polat, Peter D. Siersema, Pritam Singh, Bas Wijnhoven, Gerjon Hannink, Frans van Workum, Camiel Rosman
Summary: The Severity of oEsophageal Anastomotic Leak (SEAL) score was developed using data from the TENTACLE-Esophagus study, an international, multicentre retrospective cohort study including 1509 patients with anastomotic leak after oesophagectomy. The SEAL score was developed to determine anastomotic leak severity at diagnosis, and combines 12 leak-related parameters at diagnosis. The score may be useful in clinical practice and could improve future research.
BRITISH JOURNAL OF SURGERY
(2022)
Article
Cell & Tissue Engineering
Fanny Lebreton, Reine Hanna, Charles H. Wassmer, Kevin Bellofatto, Lisa Perez, Veronique Othenin-Girard, Begona Martinez de Tejada, Marie Cohen, Ekaterine Berishvili
Summary: Human amniotic epithelial cells possess anti-inflammatory and immunomodulatory properties which protect islets from damage induced by pro-inflammatory cytokines. This indicates that integrating hAECs in islet transplants may be a valuable strategy to inhibit inflammation-mediated islet damage and improve overall transplant success.
STEM CELL REVIEWS AND REPORTS
(2022)
