Article
Multidisciplinary Sciences
Stefanie Kurtz, Andrea Lucas-Hahn, Brigitte Schlegelberger, Gudrun Goehring, Heiner Niemann, Thomas C. Mettenleiter, Bjoern Petersen
Summary: This research explores the central role of the HMG domain in male porcine sex determination through a knockout experiment of the SRY gene with pathogenic modifications, resulting in genetically male pigs with internal female genitalia. The study could assist in solving the problem of sex preference in agriculture and establishes a more comparable large animal model for the study and development of interventions for human sex development disorders.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Chemistry, Multidisciplinary
Yuyuan Wang, Pawan K. Shahi, Xiuxiu Wang, Ruosen Xie, Yi Zhao, Min Wu, Seth Roge, Bikash R. Pattnaik, Shaoqin Gong
Summary: A stimulus-responsive silica nanoparticle has been developed for efficient delivery of nucleic acids and CRISPR genome editors, showing great potential for applications in gene therapy and genome editing.
JOURNAL OF CONTROLLED RELEASE
(2021)
Article
Biotechnology & Applied Microbiology
Jiyeon Kweon, Jung-Ki Yoon, An-Hee Jang, Ha Rim Shin, Ji-Eun See, Gayoung Jang, Jong-Il Kim, Yongsub Kim
Summary: The engineered prime editors leverage various PAM-flexible Cas9 variants to broaden the range of target sites and achieve high editing activity, successfully generating multiple types of mutations in cells. Additionally, they successfully introduce mutations such as BRAF V600E that cannot be induced by conventional prime editors, expanding the applicability of CRISPR-based prime editing technologies in biological research.
Article
Biotechnology & Applied Microbiology
Daria S. Spasskaya, Artem I. Davletshin, Stanislav S. Bachurin, Vera V. Tutyaeva, David G. Garbuz, Dmitry S. Karpov
Summary: Genomic and post-genomic editors based on CRISPR/Cas systems are widely used, but the high level of off-targets in the CRISPR/Cas9 system has hindered its application in therapeutic approaches. To overcome this obstacle, researchers have created high-fidelity Cas9 variants, but they show reduced on-target activity. A novel mutation in the PAM-interacting Cas9 domain has been found to increase the on-target activity of high-fidelity Cas9 variants while retaining their high specificity.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Ben D. Bax, Dmitry Sutormin, Neil Q. McDonald, Glenn A. Burley, Tatyana Shelkovnikova
Summary: Topoisomerases are enzymes that modify the topology of DNA to enable replication and transcription. Drugs that stabilize DNA-cleavage complexes with topoisomerases are used in cancer chemotherapy. Researchers propose a strategy to develop highly specific gene editing agents for DNA lesions causing neurodegenerative diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Zhen Liang, Yuqing Wu, Lingling Ma, Yingjie Guo, Yidong Ran
Summary: The study successfully targeted multiple genes in foxtail millet using the CRISPR/Cas9 single- and multi-gene editing systems, and generated mutant plants through genetic transformation. Base editing was achieved using two base editors, resulting in the creation of herbicide-tolerant mutant plants in foxtail millet. This system has the potential to enhance functional genomics analysis and genetic improvement in foxtail millet.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Engineering, Multidisciplinary
Hongyuan Zhang, Yifei Zhang, Wei-Xiao Wang, Weizhong Chen, Xia Zhang, Xingxu Huang, Wei Chen, Quanjiang Ji
Summary: This study identified that the Streptococcus thermophilus CRISPR associated protein 9 (St1Cas9) cytosine base editor is active in mycobacteria, allowing for C-to-T or C-to-G conversions with enhanced editing product purity and multiplexed editing capacity. Engineering improvements were made to the base editor to broaden its targeting scope and reduce efforts and time needed for precise genetic manipulation in Mycobacterium tuberculosis and related organisms.
News Item
Multidisciplinary Sciences
Heidi Ledford
Summary: Gene-therapy test marks an important year for the precise genome-editing technique, base editing.
Review
Biochemistry & Molecular Biology
Chin-Kai Chuang, Wei-Ming Lin
Summary: Cas9-sgRNA is a key tool for genome editing, with its simplicity, unique RNA-guided DNA recognition activity, and independent nuclease activities. Different variants of Cas9, in combination with sgRNA, can achieve various effects on target site breakage. Additionally, engineered enzyme activities can be introduced using Cas9 derivatives, and methods for transient expression and biosafety have been developed to address off-target effects.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biotechnology & Applied Microbiology
Alessio David Nahmad, Eli Reuveni, Ella Goldschmidt, Tamar Tenne, Meytal Liberman, Miriam Horovitz-Fried, Rami Khosravi, Hila Kobo, Eyal Reinstein, Asaf Madi, Uri Ben-David, Adi Barzel
Summary: In this study, the outcomes of genome editing in CRISPR-Cas9 transfected primary human T cells were investigated using single-cell RNA sequencing. The study revealed that chromosomal abnormalities and truncations are common outcomes of CRISPR-Cas9 cleavage, and are associated with reduced cell proliferation and cell death.
NATURE BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Olga A. Averina, Oleg A. Permyakov, Olga O. Grigorieva, Alexey S. Starshin, Alexander M. Mazur, Egor B. Prokhortchouk, Olga A. Dontsova, Petr Sergiev
Summary: Genome editing is crucial for functional genomics, but the challenge lies in the prevalence of error-prone non-homologous end joining. This study compared the efficacy of various genome editing systems on murine zygotes, finding that homologous recombination with ssDNA template following Cas9 cleavage guided by sgRNA remains the optimal method for precise genome alterations in animals.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemical Research Methods
Li Yang, Jing Tang, Xuelei Ma, Yuan Lin, Guorong Ma, Minghai Shan, Libin Wang, Yanhui Yang
Summary: Base editing technology, an efficient tool for genome editing, has been widely applied in various fields such as microorganisms, plants, animals, and medicine for basic research and therapeutics through diverse editing systems linked with different enzymes.
Article
Chemistry, Multidisciplinary
Ruosen Xie, Xiuxiu Wang, Yuyuan Wang, Mingzhou Ye, Yi Zhao, Brian S. Yandell, Shaoqin Gong
Summary: This study reports the development of a facilely fabricated pH-responsive polymer nanoparticle for safe and efficient delivery of CRISPR-associated protein 9 (Cas9) for gene editing. These nanoparticles possess many desirable properties, making them potential candidates for clinical applications in genome editing.
ADVANCED MATERIALS
(2022)
Article
Biochemistry & Molecular Biology
Shuai Wang, Chen Tao, Huilin Mao, Linghui Hou, Yao Wang, Tao Qi, Yuan Yang, Sang-Ging Ong, Shijun Hu, Renjie Chai, Yongming Wang
Summary: Due to different nucleotide preferences at target sites, no single Cas9 is capable of editing all sequences. Thus, there is a need to establish a Cas9 repertoire covering all sequences for efficient genome editing. In this study, we identified new SaCas9 orthologs with a simple NNGG PAM requirement by comparing the PAM-interacting domains in 16 SaCas9 orthologs. We also discovered three amino acids that determine the NNGG PAM requirement of SaCas9. Furthermore, we engineered high-fidelity versions of Cas9s, Sha2Cas9 and SpeCas9, which displayed high activities and distinct nucleotide preferences. Our findings offer a new perspective for expanding the Cas9 repertoire and identifying SaCas9 orthologs with NNGG PAM requirements.
Article
Materials Science, Multidisciplinary
Yue Wang, Xiujue Zheng, Jun Liu, Qixian Chen, Yan Zhao
Summary: The emergence of CRISPR-based genome editing systems has revolutionized the field of molecular biology and holds great promise for the treatment of various diseases. However, the lack of suitable delivery vehicles for effective intracellular delivery of CRISPR components limits its practical applications. In this study, a nanometer-scaled vesicle platform (CRISPRsome) was developed to efficiently deliver the essential components of the CRISPR system, resulting in significant gene knockout efficacy in cancer cells. This study highlights the potential of CRISPRsome in facilitating the use of CRISPR technology in practical applications.
ACS MATERIALS LETTERS
(2022)
Article
Surgery
Rabea Hein, Hendrik J. Sake, Claudia Pokoyski, Joachim Hundrieser, Antje Brinkmann, Wiebke Baars, Monika Nowak-Imialek, Andrea Lucas-Hahn, Constanca Figueiredo, Hans-Joachim Schuberth, Heiner Niemann, Bjoern Petersen, Reinhard Schwinzer
AMERICAN JOURNAL OF TRANSPLANTATION
(2020)
Article
Cell & Tissue Engineering
Monika Nowak-Imialek, Stephanie Wunderlich, Doris Herrmann, Svenja Breitschuh-Leibling, Gudrun Gohring, Bjoern Petersen, Sabine Klein, Ulrich Baulain, Andrea Lucas-Hahn, Ulrich Martin, Heiner Niemann
CELLULAR REPROGRAMMING
(2020)
Article
Cell Biology
Marco Oliveira, Emilio Valdivia, Murielle Verboom, Yuliia Yuzefovych, Hendrik Johannes Sake, Olena Pogozhykh, Heiner Niemann, Reinhard Schwinzer, Bjoern Petersen, Jochen Seissler, Rainer Blasczyk, Constanca Figueiredo
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
(2020)
Article
Multidisciplinary Sciences
Felix Schuster, Patrick Aldag, Antje Frenzel, Klaus-Gerd Hadeler, Andrea Lucas-Hahn, Heiner Niemann, Bjoern Petersen
SCIENTIFIC REPORTS
(2020)
Article
Medicine, Research & Experimental
Isabel Smart, Tobias Goecke, Robert Ramm, Bjoern Petersen, Doreen Lenz, Axel Haverich, Heiner Niemann, Andres Hilfiker
Summary: This study developed a sensitive dot blot protocol for detecting preformed antibodies against porcine heart valve tissue in human sera. The results showed significantly higher binding of preformed antibodies to WT tissue compared to GGTA1-KO tissue. This dot blot assay may be a valuable tool for measuring preformed antibody levels against unknown epitopes on decellularized tissue prior to implantation.
XENOTRANSPLANTATION
(2021)
Article
Multidisciplinary Sciences
Stefanie Kurtz, Andrea Lucas-Hahn, Brigitte Schlegelberger, Gudrun Goehring, Heiner Niemann, Thomas C. Mettenleiter, Bjoern Petersen
Summary: This research explores the central role of the HMG domain in male porcine sex determination through a knockout experiment of the SRY gene with pathogenic modifications, resulting in genetically male pigs with internal female genitalia. The study could assist in solving the problem of sex preference in agriculture and establishes a more comparable large animal model for the study and development of interventions for human sex development disorders.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Virology
Luise Krueger, Monika Nowak-Imialek, Yannick Kristiansen, Doris Herrmann, Bjoern Petersen, Joachim Denner
Summary: EPSCs derived from porcine preimplantation embryos exhibit higher cellular potency and lower PERV expression levels compared to other pluripotent stem cells, irrespective of pig age.
Editorial Material
Biology
Xiaolong Wang, Bjoern Petersen
SCIENCE CHINA-LIFE SCIENCES
(2022)
Article
Medicine, Research & Experimental
Lucrezia Morticelli, Mikhail Magdei, Negin Tschalaki, Bjorn Petersen, Axel Haverich, Andres Hilfiker
Summary: The study utilized digestive enzymes at different time points and dilutions to treat decellularized porcine pericardium, aiming to remove potentially immunogenic carbohydrates. The enzymatic treatments resulted in reduced lectin stainings, slightly decreased tissue strength but higher burst pressure values compared to physiological levels, which suggests a novel and efficient approach for glycan removal. Further investigations on the immunogenic potential of treated tissues are warranted in future studies.
XENOTRANSPLANTATION
(2021)
Article
Multidisciplinary Sciences
Bjoern Petersen, Robert Kammerer, Antje Frenzel, Petra Hassel, Tung Huy Dau, Roswitha Becker, Angele Breithaupt, Reiner Georg Ulrich, Andrea Lucas-Hahn, Gregor Meyers
Summary: The TRDC-locus encodes the T cell receptor delta constant region, a crucial component of the gamma delta T cell receptor. Gamma delta T cells are activated by antigens mostly independent of MHC, and their function in protection against infections is still unclear. Knocking out the porcine TRDC-locus resulted in gamma delta T cell deficient pigs, with no significant changes in other leukocyte subpopulations.
SCIENTIFIC REPORTS
(2021)
Article
Materials Science, Biomaterials
Robert Ramm, Tobias Goecke, Peter Koehler, Igor Tudorache, Serghei Cebotari, Anatol Ciubotaru, Samir Sarikouch, Klaus Hoeffler, Friederike Bothe, Bjorn Petersen, Axel Haverich, Heiner Niemann, Andres Hilfiker
Summary: The study showed that decellularized xenogeneic heart valve implants triggered strong immune responses in pigs, while allogeneic implants did not cause significant reactions. This indicates that decellularization is not effective in reducing the immunogenicity of xenogeneic implants, limiting their regenerative potential.
REGENERATIVE BIOMATERIALS
(2021)
Review
Virology
Jenny-Helena Soellner, Thomas C. Mettenleiter, Bjoern Petersen
Summary: The livestock industry faces constant threats from viral diseases, but CRISPR/Cas technology can be used to generate disease-resistant livestock, develop vaccines, and enhance understanding of virus-host interactions. Targeting genes can help reduce disease outbreaks and decrease the risk of zoonotic diseases.
Article
Biochemistry & Molecular Biology
Katharina Schmalkuche, Reinhard Schwinzer, Nadine Wenzel, Emilio Valdivia, Bjoern Petersen, Rainer Blasczyk, Constanca Figueiredo
Summary: This study demonstrated the feasibility of generating highly proliferative PTECs and developing tissue-specific immunomonitoring assays. Silencing SLA expression on PTECs was shown to be an effective strategy to prevent xenogeneic cellular immune responses and may strongly support graft survival after xenotransplantation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Cell Biology
Yiyi Xuan, Bjorn Petersen, Pentao Liu
Summary: This review comprehensively discusses the potential applications of human and pig pluripotent stem cells (PSCs) and provides an overview of the current progress and challenges. It explores the therapeutic uses of PSC-derived cellular products and emphasizes the importance of pig PSCs in genetic engineering for pig-to-human xenotransplantation. Further investigations and refinement of PSC technologies are necessary to unlock their full potential in regenerative medicine.
Article
Agriculture, Dairy & Animal Science
Shiwei Zhou, Yige Ding, Jiao Liu, Yao Liu, Xiaoe Zhao, Guanwei Li, Chenguang Zhang, Chao Li, Ying Wang, Peter Kalds, Yawei Gao, Bo Zong, Xiaoyu Huang, Shuhong Huang, Honghao Yu, Qifang Kou, Bjoern Petersen, Xingxu Huang, Xiaolong Wang, Baohua Ma, Yulin Chen
GENETICS SELECTION EVOLUTION
(2020)
Article
Reproductive Biology
Haolin Li, Xiaomeng Pei, Hao Yu, Wei Wang, Dagan Mao
Summary: This study investigated the effects of APN/AdipoRon on autophagy and apoptosis in goat luteal cells, and found that autophagy and apoptosis play important roles in the structural regression of goat corpus luteum.
Article
Reproductive Biology
Yan-Qiu Wang, He-Xuan Qu, Yan-Wei Dong, Jia-Jia Qi, Hua-Kai Wei, Hao Sun, Hao Jiang, Jia-Bao Zhang, Bo-Xing Sun, Shuang Liang
Summary: This research reveals the crucial role of Ferroptosis Suppressor Protein 1 (FSP1) in regulating the development and quality of porcine early embryos. Inhibition of FSP1 impairs blastocyst formation, induces glutathione-independent ferroptosis, and leads to oxidative stress due to mitochondrial dysfunction.
Article
Reproductive Biology
Xiong Lai, Ruizhuo Liu, Mengyu Li, Yaochun Fan, Hongxia Li, Guotao Han, Ruijie Guo, Hairui Ma, Huimin Su, Wanjin Xing
Summary: This study identifies WDR54 as a protein involved in sperm-oocyte fertilization and shows that it interacts with IZUMO1 and JUNO. The formation of the JWIJ complex on the oocyte surface suggests its importance in sperm-oocyte adhesion and fusion. This discovery provides new insights into the mechanisms of mammalian sperm-oocyte adhesion and fusion.
Article
Reproductive Biology
S. Nandi, B. Sampath Kumar, P. S. P. Gupta, S. Mondal, V. Girish Kumar
Summary: This study examined the effects of plant bioactive compounds on the survival and growth parameters of ovine preantral follicles granulosa cells (GCs) and the expression of key developmental genes. The results showed that KAE, MYR and QUE had dose-dependent responses on the morphological and functional parameters of the cells, with KAE being the most potent in augmenting ovarian functions.
Article
Reproductive Biology
Francisco E. Martin-Cano, Gemma Gaitskell-Phillips, Laura Becerro-Rey, Eva da Silva, Javier Masot, Eloy Redondo, Antonio Silva-Rodriguez, Cristina Ortega-Ferrusola, Maria Cruz Gil, Fernando J. Pena
Summary: The presence of a more efficient glycolysis mechanism depending on pyruvate in stallion spermatozoa can counteract the detrimental effects of higher glucose concentrations. Incubating spermatozoa with 10 mM pyruvate in a modified Tyrode's media with 67 mM glucose showed increased motility and maintained viability. This improvement is attributed to the conversion of lactate to pyruvate and increased NAD(+) enhancing glycolysis efficiency.
Article
Reproductive Biology
Thanida Sananmuang, Denis Puthier, Catherine Nguyen, Kaj Chokeshaiusaha
Summary: Ongoing progress in mRNA-Sequencing technologies has contributed to the refinement of assisted reproductive technologies. This study utilized mRNA-Seq datasets of various mammalian species to analyze differential transcript usage (DTU) and identified ten cross-species DTU genes. Gene Ontology (GO) term enrichment analysis revealed the involvement of these genes in RNA and cell-cycle control mechanisms, suggesting their importance during oocyte maturation. Further exploration of these genes' transcript isoforms may lead to the discovery of novel markers for reproductive technologies.
Article
Reproductive Biology
Chenglei Song, Zhe Zhang, Yilin Wei, Yaqing Dou, Kunlong Qi, Xiuling Li, Feng Yang, Xinjian Li, Kejun Wang, Ruimin Qiao, Xuelei Han
Summary: In this study, the changes in protein levels in boar sperm with different liquid preservation abilities were analyzed. Key proteins related to sperm liquid preservation ability were identified. The findings provide new insights into the molecular mechanisms of boar sperm liquid preservation ability.
Article
Reproductive Biology
Genevieve M. VanWye, Emily G. Smith, Christine Spinka, Michael F. Smith, Matthew C. Lucy, Jordan M. Thomas
Summary: A series of experiments evaluated treatment schedules for controlling the estrous cycle in beef heifers. The results showed that the duration of progestin treatment and the timing of prostaglandin administration had an impact on estrus expression and pregnancy rates.
Article
Reproductive Biology
Hongwei Duan, Shuai Yang, Longfei Xiao, Shanshan Yang, Zhenxing Yan, Fang Wang, Xiaofei Ma, Lihong Zhang, Yong Zhang, Junjie Hu, Xingxu Zhao
Summary: This study first elucidated the mechanism by which melatonin regulates progesterone secretion in the corpus luteum of sheep, namely by promoting progesterone secretion through the regulation of autophagy. This has important implications for further exploring the role of melatonin in regulating sheep ovarian function.
Article
Reproductive Biology
Zhijie Zheng, Yingfang Guo, Yonghui Zheng, Haichong Wu
Summary: This study demonstrates that miR-92b plays a protective role in LTA-induced endometritis by targeting FZD10 and reducing the production of pro-inflammatory cytokines, thus alleviating uterine injury.
Article
Reproductive Biology
Areeg Almubarak, Sanghoon Lee, Il-Jeoung Yu, Yubyeol Jeon
Summary: This study found that Nobiletin (NOB) can promote the cryosurvival and embryo development of porcine sperm after in vitro fertilization (IVF). The addition of NOB improves sperm motility, acrosome integrity, viability, and mitochondrial membrane potential, reduces lipid peroxidation, and increases cleavage and blastocyst formation rates. Furthermore, NOB treatment upregulates the expression of genes related to pluripotency and cell differentiation in the resulting embryos.
Article
Reproductive Biology
Yuan Li, Lishu Li, Wenjie Xiong, Xing Duan, Huaming Xi
Summary: This study investigated the cytotoxicity and toxicological mechanisms of fluorochloridone (FLC) on cultured goat Sertoli cells. The results showed that FLC exposure induced oxidative stress, promoted apoptosis and autophagy, inhibited lysosomal biogenesis, and blocked autophagic flux in goat Sertoli cells. FLC also caused mitochondrial dysfunction and disturbed mitophagy, leading to cell apoptosis. The antioxidant N-acetyl-L-cysteine (NAC) reduced FLC-induced ROS accumulation and reversed the disorder of autophagy levels.
Article
Reproductive Biology
Gabriela Ramos Leal, Thais de Almeida Oliveira, Mariana Pedrosa de Paula Guimaraes, Lucas Francisco Leodido Correia, Erlandia Marcia Vasconcelos, Joanna Maria Goncalves Souza-Fabjan
Summary: This study investigated the time course of lipid accumulation during IVM and the effects of lipid modulators on cat oocytes. The results showed that adding lipid modulators can reduce lipid content and improve viability after cryopreservation.
Article
Reproductive Biology
Isabella Rio Feltrin, Amanda Guimaraes da Silva, Cecilia Constantino Rocha, Priscila Assis Ferraz, Paola Maria da Silva Rosa, Thiago Martins, Juliano Coelho da Silveira, Milena Lopes Oliveira, Mario Binelli, Guilherme Pugliesi, Claudia Maria Bertan Membrive
Summary: The study evaluated the effects of E2 treatment on Nelore heifers on day 15 of the estrous cycle, finding that E2 promoted an increase in PGFM concentrations and accelerated functional and structural luteolysis by upregulating the expression of PGR and OXTR. This suggests that within 3 hours after E2 stimulus, the expression of these receptors is associated with triggering luteolysis in cattle.
Article
Reproductive Biology
Aline Matos Arrais, Angelo Jose Burla Dias, Claudio Luiz Melo de Souza, Alinne Gloria Curcio, Marco Roberto Bourg de Mello
Summary: This study evaluated the effect of phospholipase C (PLC) on the capacitation of cryopreserved ovine semen. The results showed that the inhibitor U73122 can prevent premature capacitation and acrosome reaction induced by the freezing procedure, without affecting the kinetics and integrity of the sperm membranes.
