Exploiting nanobodies and Affimers for superresolution imaging in light microscopy

Nanobody Detection of Standard Fluorescent Proteins Enables Multi-Target DNA-PAINT with High Resolution and Minimal Displacement Errors

(2019) Shama Sograte-Idrissi et al.   Cells

Site-Specific Labeling of Affimers for DNA-PAINT Microscopy

(2018) Thomas Schlichthaerle et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

The Effect of Fluorophore Conjugation on Antibody Affinity and the Photophysical Properties of Dyes

(2018) Ágnes Szabó et al.   BIOPHYSICAL JOURNAL

Recent advances in the selection and identification of antigen-specific nanobodies

(2018) Wenshuai Liu et al.   MOLECULAR IMMUNOLOGY

High-resolution cryo-EM structures of actin-bound myosin states reveal the mechanism of myosin force sensing

(2018) Ahmet Mentes et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Fluorescent dyes and probes for super-resolution microscopy of microtubules and tracheoles in living cells and tissues

(2018) Gražvydas Lukinavičius et al.   Chemical Science

A peptide tag-specific nanobody enables high-quality labeling for dSTORM imaging

(2018) David Virant et al.   Nature Communications

Affimer proteins for F-actin: novel affinity reagents that label F-actin in live and fixed cells

(2018) Anna Lopata et al.   Scientific Reports

Rapid and efficient C-terminal labeling of nanobodies for DNA-PAINT

(2018) Valentin Fabricius et al.   JOURNAL OF PHYSICS D-APPLIED PHYSICS

Nanobodies reveal an extra-synaptic population of SNAP-25 and Syntaxin 1A in hippocampal neurons

(2018) Manuel Maidorn et al.   mAbs

Super-resolution microscopy demystified

(2018) Lothar Schermelleh et al.   NATURE CELL BIOLOGY

Nanobody immunostaining for correlated light and electron microscopy with preservation of ultrastructure

(2018) Tao Fang et al.   NATURE METHODS

A toolbox of anti–mouse and anti–rabbit IgG secondary nanobodies

(2017) Tino Pleiner et al.   JOURNAL OF CELL BIOLOGY

Affimer proteins inhibit immune complex binding to FcγRIIIa with high specificity through competitive and allosteric modes of action

(2017) James I. Robinson et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Isolation of isoform-specific binding proteins (Affimers) by phage display using negative selection

(2017) Anna Ah-San Tang et al.   Science Signaling

Generation of specific inhibitors of SUMO-1– and SUMO-2/3–mediated protein-protein interactions using Affimer (Adhiron) technology

(2017) David J. Hughes et al.   Science Signaling

Affimer proteins are versatile and renewable affinity reagents

(2017) Christian Tiede et al.   eLife

Isolation of isoform-specific binding proteins (Affimers) by phage display using negative selection

(2017) Anna Ah-San Tang et al.   Science Signaling

Generation of specific inhibitors of SUMO-1– and SUMO-2/3–mediated protein-protein interactions using Affimer (Adhiron) technology

(2017) David J. Hughes et al.   Science Signaling

NaLi-H1: A universal synthetic library of humanized nanobodies providing highly functional antibodies and intrabodies

(2016) Sandrine Moutel et al.   eLife

Single-molecule microscopy of molecules tagged with GFP or RFP derivatives in mammalian cells using nanobody binders

(2015) Evgenia Platonova et al.   METHODS

Multitarget super-resolution microscopy with high-density labeling by exchangeable probes

(2015) Tai Kiuchi et al.   NATURE METHODS

Non-immunoglobulin scaffolds: a focus on their targets

(2015) Katja Škrlec et al.   TRENDS IN BIOTECHNOLOGY

Resolving bundled microtubules using anti-tubulin nanobodies

(2015) Marina Mikhaylova et al.   Nature Communications

Nanobodies: site-specific labeling for super-resolution imaging, rapid epitope-mapping and native protein complex isolation

(2015) Tino Pleiner et al.   eLife

Adhiron: a stable and versatile peptide display scaffold for molecular recognition applications

(2014) Christian Tiede et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Nanobodies: Natural Single-Domain Antibodies

(2013) Serge Muyldermans   Annual Review of Biochemistry

A simple, versatile method for GFP-based super-resolution microscopy via nanobodies

(2012) Jonas Ries et al.   NATURE METHODS

Aptamers as potential tools for super-resolution microscopy

(2012) Felipe Opazo et al.   NATURE METHODS

Dual-objective STORM reveals three-dimensional filament organization in the actin cytoskeleton

(2012) Ke Xu et al.   NATURE METHODS

Super-resolution fluorescence imaging of organelles in live cells with photoswitchable membrane probes

(2012) S.-H. Shim et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

DARPins and other repeat protein scaffolds: advances in engineering and applications

(2011) Ykelien L Boersma et al.   CURRENT OPINION IN BIOTECHNOLOGY

Fluorescent-labeled antibodies: Balancing functionality and degree of labeling

(2010) Shaleen Vira et al.   ANALYTICAL BIOCHEMISTRY

Affibody molecules: Engineered proteins for therapeutic, diagnostic and biotechnological applications

(2010) J. Löfblom et al.   FEBS LETTERS

Super-Resolution Imaging of DNA Labelled with Intercalating Dyes

(2009) Cristina Flors et al.   CHEMPHYSCHEM