Article
Multidisciplinary Sciences
Kendi Okuda, Miriam Maria Silva Costa Franco, Ari Yasunaga, Ricardo Gazzinelli, Michel Rabinovitch, Sara Cherry, Neal Silverman
Summary: Leishmania parasites utilize virulence mechanisms to invade and replicate in macrophages, inhibiting host defenses and altering gene expression to enhance their survival and replication.
Article
Immunology
Raiza Raianne Luz Rodrigues, Thais Amanda Lima Nunes, Alyne Rodrigues de Araujo, Jose Delano Barreto Marinho Filho, Marcos Vinicius da Silva, Fernando Aecio de Amorim Carvalho, Otilia Deusdenia Loiola Pessoa, Hozana Patricia Silva Freitas, Klinger Antonio da Franca Rodrigues, Ana Jersia Araujo
Summary: The study demonstrated that CORe from Cordia polycephala has antileishmanial activity, being effective against the parasite while non-toxic to host cells. It showed greater efficacy in inhibiting amastigote growth and higher selectivity indexes compared to reference drugs.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2021)
Article
Multidisciplinary Sciences
Andreza do Socorro Silva da Veiga, Fernando Tobias Silveira, Edilene Oliveira da Silva, Jose Antonio Picanco Diniz Junior, Sanderson Correa Araujo, Marliane Batista Campos, Andrey Moacir do Rosario Marinho, Geraldo Celio Brandao, Valdicley Vieira Vale, Sandro Percario, Maria Fani Dolabela
Summary: This study evaluated the morphological changes caused by fractions and subfractions obtained from barks of Aspidosperna nitidum against L. (L.) amazonensis promastigotes. The results suggest that alkaloids isolated from A. nitidum show potential as leishmanicidal agents. Scanning electron microscopy and transmission electron microscopy analysis revealed changes in the parasites' morphology after treatment with the active fractions.
SCIENTIFIC REPORTS
(2022)
Article
Pharmacology & Pharmacy
Jamie L. Arnst, George R. Beck
Summary: Phosphorus, essential to cellular function, can promote cancer when available in excess. Manipulating phosphorus consumption could be a novel approach for cancer therapy.
BIOCHEMICAL PHARMACOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Carlos J. Bethencourt-Estrella, Samuel Delgado-Hernandez, Atteneri Lopez-Arencibia, Desiree San Nicolas-Hernandez, David Tejedor, Fernando Garcia-Tellado, Jacob Lorenzo-Morales, Jose E. Pinero
Summary: Leishmaniasis is one of the most important tropical diseases, with approximately one million new cases each year. Current treatments are not fully effective and are toxic, so there is a need to develop new therapies that are more effective and less toxic. This study found that certain acrylonitrile derivatives have good selectivity in killing Leishmania parasites and induce programmed cell death.
BIOORGANIC CHEMISTRY
(2022)
Article
Pharmacology & Pharmacy
Juliana Figueiredo Peixoto, Luiz Filipe Goncalves-Oliveira, Franklin Souza-Silva, Luzia Monteiro de Castro Cortes, Geovane Dias-Lopes, Flavia de Oliveira Cardoso, Rafael de Oliveira Santos, Beatriz Ferreira de Carvalho Patricio, Caroline Deckmann Nicoletti, Carolina Guimaraes de Souza Lima, Katia da Silva Calabrese, Davyson de Lima Moreira, Helvecio Vinicius Antunes Rocha, Fernando de Carvalho da Silva, Vitor Francisco Ferreira, Carlos Roberto Alves
Summary: Oil-in-water microemulsions loaded with epoxy-alpha-lapachone (ELAP-ME) were successfully developed and showed good stability. ELAP-ME demonstrated promising activity against murine infection with Leishmania (Leishmania) amazonensis.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2023)
Article
Microbiology
Simone S. C. Oliveira, Vanessa S. S. Santos, Michael Devereux, Malachy McCann, Andre L. S. Santos, Marta H. H. Branquinha
Summary: In this study, the effects of silver(I) and copper(II) complexes of 1,10-phenanthroline-5,6-dione (phendione) were evaluated as potential drugs for the treatment of Leishmania infection. The complexes demonstrated significant inhibitory effects on parasite proliferation and induced programmed cell death. They also showed potent activity against intracellular parasites and low toxicity to host cells.
Article
Immunology
Liliane Sena Pinheiro, Valter Viana Andrade-Neto, Marcio Mantuano-Barradas, Elisa Cavalcante Pereira, Rodrigo Cesar Fernandes Barbosa, Marcia Cristina Campos de Oliveira, Rubem Figueiredo Sadok Menna-Barreto, Edezio Ferreira Cunha-Junior, Eduardo Caio Torres-Santos
Summary: The study investigated the effects of exogenously added trans, trans-farnesol on the proliferation ability of Leishmania amazonensis. The results showed that trans, trans-farnesol inhibited the cell cycle and proliferation of L. amazonensis without causing DNA fragmentation or loss of mitochondrial functionality. Additionally, the study identified the presence of alpha-farnesene and beta-farnesene in the lipid secretome of the parasite and identified the farnesene synthase gene responsible for their production.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Immunology
Thais Amanda de Lima Nunes, Malu Mateus Santos, Mariana Silva de Oliveira, Julyanne Maria Saraiva de Sousa, Raiza Raianne Luz Rodrigues, Paulo Sergio de Araujo Sousa, Alyne Rodrigues de Araujo, Anna Carolina Toledo da Cunha Pereira, Gustavo Portela Ferreira, Jefferson Almeida Rocha, Virmondes Rodrigues Junior, Marcos Vinicius da Silva, Klinger Antonio da Franca Rodrigues
Summary: Curzerene, a component of Eugenia uniflora essential oil, showed effective and selective antileishmanial activity against promastigotes and amastigotes. It induced apoptosis and secondary necrosis in parasites, and demonstrated immunomodulatory effects in infected macrophages.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2021)
Article
Biophysics
Daniela Coelho, Beatriz Veleirinho, Leticia Mazzarino, Thais Alberti, Elizandra Buzanello, Regina Eva Oliveira, Rosendo Augusto Yunes, Milene Moraes, Mario Steindel, Marcelo Maraschin
Summary: Cutaneous leishmaniasis is a global public health issue, and the use of synthetic chalcones shows promise in developing molecules with anti-leishmaniasis activity.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2021)
Review
Immunology
Matheus B. Carneiro, Nathan C. Peters
Summary: This review focuses on the interaction between Leishmania amazonensis infection and host innate immune response, particularly addressing the impact of adaptive immunity on phagocytic host cell recruitment and activation. Experimental observations indicate that most mouse strains are susceptible to L. amazonensis infection, unlike other Leishmania species. The CD4(+) T helper (Th)1/Th2 paradigm does not fully explain the chronic disease established by L. amazonensis, with recent findings complicating the understanding of the Th1/Th2 paradigm.
FRONTIERS IN IMMUNOLOGY
(2021)
Review
Parasitology
Taylon Felipe Silva, Fernanda Tomiotto-Pellissier, Aline Kuhn Sbruzzi Pasquali, Fernanda Pinto-Ferreira, Wander Rogerio Pavanelli, Ivete Conchon-Costa, Italmar Teodorico Navarro, Eloiza Teles Caldart
Summary: The study revealed that the exact mechanism leading to different clinical manifestations among strains of the same species is still uncertain, and future studies are needed to better elucidate this phenomenon.
Article
Biochemistry & Molecular Biology
Rafael M. Oliveira, Thaise L. Teixeira, Cassiano C. Rodrigues, Aline A. da Silva, Bruna C. Borges, Rebecca T. S. Brigido, Samuel C. Teixeira, Marlus A. Dos Santos, Joao Paulo S. Servato, Debora de O. Santos, Marcelo J. B. Silva, Luiz R. Goulart, Claudio da Silva
Summary: The study revealed the critical role of gal-3 in the host response to infection by L. (L.) amazonensis, controlling parasite invasion, replication, and formation of endocytic vesicles. Mice with gal-3 deficiency showed an exacerbated inflammatory response.
Article
Microbiology
Brenda Furtado Costa, Tarcisio Navegante de Queiroz Filho, Adeniele Lopes da Cruz Carneiro, Aline Semblano Carreira Falcao, Maria Sueli da Silva Kataoka, Joao de Jesus Viana Pinheiro, Ana Paula Drummond Rodrigues
Summary: This study examines the presence and activity of MMP-2 and MMP-9 in Leishmania parasites, particularly in two different Leishmania species, L. amazonensis and L. braziliensis. The results show that MMP-9 has significantly higher gelatinolytic activity in L. amazonensis compared to L. braziliensis. The study suggests that MMP-2 and MMP-9 may play a role in the infection dynamics of these Leishmania species and could be potential markers for severe leishmaniasis and therapeutic targets.
Article
Chemistry, Medicinal
Atteneri Lopez-Arencibia, Ines Sifaoui, Maria Reyes-Batlle, Carlos J. Bethencourt-Estrella, Desiree San Nicolas-Hernandez, Jacob Lorenzo-Morales, Jose E. Pinero
Summary: This study investigated the anti-leishmanial activity of five Pathogen Box compounds against Leishmania amazonensis, showing that three of them significantly inhibited parasite proliferation and induced cell death through different mechanisms. These findings highlight the potential of these compounds as novel therapeutic agents for leishmaniasis.
Article
Biochemistry & Molecular Biology
Brian S. Mantilla, Lisvane S. Paes, Elizabeth M. F. Pral, Daiana E. Martil, Otavio H. Thiemann, Patricio Fernandez-Silva, Erick L. Bastos, Ariel M. Silber
JOURNAL OF BIOLOGICAL CHEMISTRY
(2015)
Article
Parasitology
Anita Leocadio Freitas-Mesquita, Marta T. Gomes, Danielle P. Vieira, Lisvane Paes-Vieira, Michelle T. C. Nascimento, Angela H. C. S. Lopes, Jose Roberto Meyer-Fernandes
EXPERIMENTAL PARASITOLOGY
(2016)
Review
Cell Biology
Lisvane Paes-Vieira, Andre Luiz Gomes-Vieira, Jose Roberto Meyer-Fernandes
CELL BIOLOGY INTERNATIONAL
(2018)
Article
Genetics & Heredity
Andre L. Gomes-Vieira, Jeremy G. Wideman, Lisvane Paes-Vieira, Suely L. Gomes, Thomas A. Richards, Jose Roberto Meyer-Fernandes
FUNGAL GENETICS AND BIOLOGY
(2018)
Article
Genetics & Heredity
Andre L. Gomes-Vieira, Lisvane Paes-Vieira, Dayana K. B. B. Zamboni, Andre L. A. Dos-Santos, Claudia F. Dick, Jose Roberto Meyer-Fernandes
FUNGAL GENETICS AND BIOLOGY
(2018)
Article
Parasitology
Naira Ligia Lima Giarola, Thais Souza Silveira, Job Domingos Filho Inacio, Lisvane Paes Vieira, Elmo Eduardo Almeida-Amaral, Jose Roberto Meyer-Fernandes
EXPERIMENTAL PARASITOLOGY
(2014)
Article
Biochemistry & Molecular Biology
Michele Alves-Bezerra, Daniela Cosentino-Gomes, Lisvane P. Vieira, Nathalia Rocco-Machado, Katia C. Gondim, Jose R. Meyer-Fernandes
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2014)
Article
Multidisciplinary Sciences
Andre Luiz Fonseca-de-Souza, Anita Leocadio Freitas-Mesquita, Lisvane Paes Vieira, David Majerowicz, Nathalia Daflon-Yunes, Lia Carolina Almeida Soares-de-Medeiros, Kildare Miranda, Katia Calp Gondim, Jose Roberto Meyer-Fernandes
Article
Parasitology
Andre L. A. Dos-Santos, Claudia F. Dick, Leandro R. Lopes, Nathalia Rocco-Machado, Humberto Muzi-Filho, Anita L. Freitas-Mesquita, Lisvane Paes-Vieira, Adalberto Vieyra, Jose Roberto Meyer-Fernandez
EXPERIMENTAL PARASITOLOGY
(2019)
Article
Biochemistry & Molecular Biology
N. Rocco-Machado, D. Cosentino-Gomes, M. T. Nascimento, L. Paes-Vieira, Y. A. Khan, B. Mittra, N. W. Andrews, J. R. Meyer-Fernandes
FREE RADICAL BIOLOGY AND MEDICINE
(2019)
Article
Biochemistry & Molecular Biology
Felipe B. Saraiva, Michele Alves-Bezerra, David Majerowicz, Lisvane Paes-Vieira, Valdir Braz, Muriel G. M. D. Almeida, Jose Roberto Meyer-Fernandes, Katia C. Gondim
Summary: In this study, the regulation of R. prolixus ACC expression and de novo lipogenesis activity in adult females under different nutritional and metabolic conditions was investigated. The blood meal induces the utilization of diet-derived amino acids by de novo lipogenesis in the fat body, and the control of this activity does not occur at the RhoprACC gene or protein expression level. These results provide insights into the synthesis and regulation of lipids in insects, particularly in the context of blood feeding and metabolic changes.
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Brian S. Mantilla, Lisvane Paes-Vieira, Felipe de Almeida Dias, Simone G. Calderano, Maria Carolina Elias, Daniela Cosentino-Gomes, Pedro L. Oliveira, Jose Roberto Meyer-Fernandes, Ariel M. Silber
Summary: The pathogenic protist Trypanosoma cruzi utilizes kissing bugs as invertebrate hosts to vectorize infection among mammals. Through the proline-glutamate oxidation pathway, mitochondrial enzymes play a crucial role in differentiation and energy production in the parasite. Overexpression of these enzymes leads to increased mitochondrial functions and different oxygen signaling, influencing parasitic differentiation efficiency in the invertebrate host.
BIOCHEMICAL JOURNAL
(2021)
Article
Parasitology
Marta Teixeira Gomes, Lisvane Paes-Vieira, Andre Luiz Gomes-Vieira, Daniela Cosentino-Gomes, Ana Paula Pereira da Silva, Naira Ligia Lima Giarola, Daniel Da Silva, Mauro Sola-Penna, Antonio Galina, Jose Roberto Meyer-Fernandes
Summary: 3-Bromopyruvate (3-BrPA) significantly reduced glycolytic enzyme activity in L. amazonensis, leading to decreased ATP levels and energy depletion, thereby inhibiting cell proliferation and infectivity.
EXPERIMENTAL PARASITOLOGY
(2021)
Article
Parasitology
Lisvane Paes-Vieira, Nathalia Rocco-Machado, Anita Leocadio Freitas-Mesquita, Yago Sousa dos Santos Emiliano, Andre Luiz Gomes-Vieira, Elmo Eduardo de Almeida-Amaral, Jose Roberto Meyer-Fernandes
Summary: The study reveals that the activity of E-NTPDases in Leishmania amazonensis is differentially regulated during parasite development, along with the expression levels of ntpd1 and ntpd2 genes. Parasites overexpressing ntpd2 gene show increased interaction with macrophages and higher infectivity compared to controls. Interestingly, lesions caused by ntpd1 and ntpd2-overexpressing parasites were significantly reduced after twenty days post-infection, despite similar parasite loads among experimental groups, suggesting a non-linear relationship between E-NTPDase activity and lesion formation.
PARASITOLOGY INTERNATIONAL
(2021)
