CRISPR/Cas9-assisted gRNA-free one-step genome editing with no sequence limitations and improved targeting efficiency
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Title
CRISPR/Cas9-assisted gRNA-free one-step genome editing with no sequence limitations and improved targeting efficiency
Authors
Keywords
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Journal
Scientific Reports
Volume 7, Issue 1, Pages -
Publisher
Springer Nature
Online
2017-11-24
DOI
10.1038/s41598-017-16998-8
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Note: Only part of the references are listed.- A Novel and Efficient Method for Bacteria Genome Editing Employing both CRISPR/Cas9 and an Antibiotic Resistance Cassette
- (2017) Hong Zhang et al. Frontiers in Microbiology
- Development of a fast and easy method for Escherichia coli genome editing with CRISPR/Cas9
- (2016) Dongdong Zhao et al. Microbial Cell Factories
- Multigene Editing in the Escherichia coli Genome via the CRISPR-Cas9 System
- (2015) Yu Jiang et al. APPLIED AND ENVIRONMENTAL MICROBIOLOGY
- Systematic analysis of CRISPR–Cas9 mismatch tolerance reveals low levels of off-target activity
- (2015) Emily M. Anderson et al. JOURNAL OF BIOTECHNOLOGY
- Metabolic engineering of Escherichia coli using CRISPR–Cas9 meditated genome editing
- (2015) Yifan Li et al. METABOLIC ENGINEERING
- Engineered CRISPR-Cas9 nucleases with altered PAM specificities
- (2015) Benjamin P. Kleinstiver et al. NATURE
- Broadening the targeting range of Staphylococcus aureus CRISPR-Cas9 by modifying PAM recognition
- (2015) Benjamin P Kleinstiver et al. NATURE BIOTECHNOLOGY
- CRISPRdirect: software for designing CRISPR/Cas guide RNA with reduced off-target sites
- (2014) Yuki Naito et al. BIOINFORMATICS
- Cas-OFFinder: a fast and versatile algorithm that searches for potential off-target sites of Cas9 RNA-guided endonucleases
- (2014) S. Bae et al. BIOINFORMATICS
- E-CRISP: fast CRISPR target site identification
- (2014) Florian Heigwer et al. NATURE METHODS
- One-Step Generation of Mice Carrying Mutations in Multiple Genes by CRISPR/Cas-Mediated Genome Engineering
- (2013) Haoyi Wang et al. CELL
- Genome editing with RNA-guided Cas9 nuclease in Zebrafish embryos
- (2013) Nannan Chang et al. CELL RESEARCH
- Analysis of off-target effects of CRISPR/Cas-derived RNA-guided endonucleases and nickases
- (2013) S. W. Cho et al. GENOME RESEARCH
- Engineering central metabolic modules of Escherichia coli for improving β-carotene production
- (2013) Jing Zhao et al. METABOLIC ENGINEERING
- Targeted genome engineering in human cells with the Cas9 RNA-guided endonuclease
- (2013) Seung Woo Cho et al. NATURE BIOTECHNOLOGY
- Efficient genome editing in zebrafish using a CRISPR-Cas system
- (2013) Woong Y Hwang et al. NATURE BIOTECHNOLOGY
- High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells
- (2013) Yanfang Fu et al. NATURE BIOTECHNOLOGY
- RNA-guided editing of bacterial genomes using CRISPR-Cas systems
- (2013) Wenyan Jiang et al. NATURE BIOTECHNOLOGY
- Efficient generation of large-scale genome-modified mice using gRNA and CAS9 endonuclease
- (2013) Wataru Fujii et al. NUCLEIC ACIDS RESEARCH
- Protospacer recognition motifs
- (2013) Shiraz A. Shah et al. RNA Biology
- Genome-Scale CRISPR-Cas9 Knockout Screening in Human Cells
- (2013) Ophir Shalem et al. SCIENCE
- A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity
- (2012) M. Jinek et al. SCIENCE
- De novo-engineered transcription activator-like effector (TALE) hybrid nuclease with novel DNA binding specificity creates double-strand breaks
- (2011) M. M. Mahfouz et al. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
- j5 DNA Assembly Design Automation Software
- (2011) Nathan J. Hillson et al. ACS Synthetic Biology
- A TALE nuclease architecture for efficient genome editing
- (2010) Jeffrey C Miller et al. NATURE BIOTECHNOLOGY
- Genome editing with engineered zinc finger nucleases
- (2010) Fyodor D. Urnov et al. NATURE REVIEWS GENETICS
- Short motif sequences determine the targets of the prokaryotic CRISPR defence system
- (2009) MICROBIOLOGY-SGM
- Programming cells by multiplex genome engineering and accelerated evolution
- (2009) Harris H. Wang et al. NATURE
- A One Pot, One Step, Precision Cloning Method with High Throughput Capability
- (2008) Carola Engler et al. PLoS One
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