Article
Biochemistry & Molecular Biology
Wenqiang Fan, Jeronimo Jurado-Arjona, Gregorio Alanis-Lobato, Sophie Peron, Christian Berger, Miguel A. Andrade-Navarro, Sven Falk, Benedikt Berninger
Summary: Most adult hippocampal neural stem cells (NSCs) remain quiescent, but the activity of the transcriptional co-activator Yap1 is enriched in active NSCs. Genetic deletion of Yap1 reduces the proportion of active NSCs, indicating its role in regulating the transition from quiescence to activation. Overexpression of a gain-of-function Yap1 mutant leads to cell cycle entry in NSCs and astrocytes, and dysregulated Yap1 activity represses neurogenesis and induces glioblastoma stem cell-like characteristics.
Article
Neurosciences
Marta Gronksa-Peski, J. Tiago Goncalves, Jean M. Hebert
Summary: Research shows that an enriched environment promotes adult neurogenesis by increasing the function of fibroblast growth factor receptor (FGFR) within neurogenic cells. Activation of FGFR by an enriched environment signals through FGFR substrate (FRS) to induce stem cell proliferation and via FRS and phospholipase C gamma to increase the number of adult-born neurons.
JOURNAL OF NEUROSCIENCE
(2021)
Article
Cell Biology
Shao-Qi Zhang, Qiao Deng, Qi Zhu, Zhuang-Li Hu, Li-Hong Long, Peng-Fei Wu, Jin-Gang He, Hong-Sheng Chen, Zhenyu Yue, Jia-Hong Lu, Fang Wang, Jian-Guo Chen
Summary: This study reveals the important role of dysfunctional autophagy in the pathogenesis of major depressive disorder (MDD). The expression of NRBF2 is attenuated in the dentate gyrus (DG) under chronic stress and its deficiency impairs autophagic flux in adult neural stem cells (aNSCs). Loss of NRBF2 disrupts the neurogenesis-related protein network and leads to the depression-like phenotype, while overexpressing NRBF2 rescues impaired adult hippocampal neurogenesis (AHN) and depression-like phenotype in mice.
Article
Cell Biology
Laura Garcia-Corzo, Isabel Calatayud-Baselga, Lucia Casares-Crespo, Carlos Mora-Martinez, Juan Julian Escribano-Saiz, Rafael Hortigueela, Andrea Asenjo-Martinez, Antonio Jordan-Pla, Stefano Ercoli, Nuria Flames, Victoria Lopez-Alonso, Marcal Vilar, Helena Mira
Summary: The study reveals the role of the transcription factor LEF1 in the gene regulation of quiescent NSPCs. It also highlights the significance of alternative splicing as a post-transcriptional regulatory mechanism in the transition from stem cell activation to quiescence.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Alexandra Poetzsch, Sara Zocher, Stefanie N. Bernas, Odette Leiter, Annette E. Ruenker, Gerd Kempermann
Summary: The study showed that L-lactate has a pro-proliferative effect on neural precursor cells, which is dependent on the active lactate transport by monocarboxylate transporters and is not linked to amplified mitochondrial respiration. L-lactate redirected glucose metabolism to the pentose phosphate pathway, as indicated by increased glucose-6-phosphate dehydrogenase activity and decreased glycolysis.
Article
Neurosciences
Dmitry I. Maltsev, Victor A. Aniol, Mariia A. Golden, Anastasia D. Petrina, Vsevolod V. Belousov, Natalia V. Gulyaeva, Oleg V. Podgorny
Summary: The delicate balance between quiescence and division of radial glia-like stem cells is crucial for adult hippocampal neurogenesis. This study demonstrates that long-term stimulation of quiescent RGLs using memantine or electroconvulsive seizure leads to premature depletion and altered neurogenesis. Additionally, aging affects the ability of quiescent RGLs to be recruited by these inducers.
MOLECULAR NEUROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Greer S. Kirshenbaum, Chia-Yuan Chang, Maria Bompolaki, Victoria R. Bradford, Joseph Bell, Stylianos Kosmidis, Rebecca M. Shansky, Javier Orlandi, Lisa M. Savage, Alexander Z. Harris, E. David Leonardo, Alex Dranovsky
Summary: Adult neurogenesis is reduced during aging and impaired in stress, memory, and cognition disorders, but its normal function is still unclear. This study found that long-term suppression of neurogenesis led to a decline in hippocampal acetylcholine signaling and working memory deficits. However, compensatory reorganization of the cholinergic dentate gyrus inputs and recruitment of different neuronal projections helped correct the memory dysfunction. These findings highlight the importance of hippocampal neurogenesis in memory maintenance and provide insights into the progressive nature of memory deterioration.
MOLECULAR PSYCHIATRY
(2023)
Article
Multidisciplinary Sciences
Joshua D. Rieskamp, Ileanexis Rosado-Burgos, Jacob E. Christofi, Eliza Ansar, Dalia Einstein, Ashley E. Walters, Valentina Valentini, John P. Bruno, Elizabeth D. Kirby
Summary: This study demonstrates that glutamate stimulates neural stem cells (NSCs) to self-renew and proliferate through excitatory amino acid transporter 1 (EAAT1), which also stimulates lipogenesis. Transcriptomics and pathway manipulation reveal the underlying mechanism of this process.
Article
Cell & Tissue Engineering
Kyung-Joo Seong, Seungho Choi, Hyun-Gwan Lee, Joon Haeng Rhee, Jin Ho Lee, Jeong-Tae Koh, Sun-Hun Kim, Won-Seok Choi, Ji-Yeon Jung, Won-Jae Kim
Summary: Toll-like receptor 5 plays a crucial role in regulating neurogenesis from mouse embryonic stem cells and adult hippocampal neural stem cells, promoting neural differentiation and inhibiting cell proliferation. TLR5 deficiency impairs fear memory performance in mice.
Article
Biochemistry & Molecular Biology
Liying Chen, Yingwei Xu, Heming Cheng, Zhongxia Li, Nanxi Lai, Menghan Li, Yeping Ruan, Yang Zheng, Fan Fei, Cenglin Xu, Jiao Ma, Shuang Wang, Yan Gu, Feng Han, Zhong Chen, Yi Wang
Summary: This study found that adult-born dentate granule cells (abDGCs) born at a critical period after epileptogenic insult can influence the duration of seizures. Activation or inhibition of these cells can significantly extend or curtail seizure duration, providing new insights for precise treatment of epilepsy.
SIGNAL TRANSDUCTION AND TARGETED THERAPY
(2023)
Article
Biochemistry & Molecular Biology
Esra Albayrak, Sezer Akgol, Raife Dilek Turan, Merve Uslu, Fatih Kocabas
Summary: This study identified and investigated 35 small molecules that target hematopoietic stem cell (HSC) quiescence factors. The molecules BML-260 and TAME were found to significantly increase HSC content and activate cell cycle re-entry. These molecules promoted HSC expansion by modulating cell cycle kinetics. HSCs expanded with BML-260 showed high reconstitution capacity and increased immune cell content after xenotransplantation into immunocompromised mice.
JOURNAL OF CELLULAR BIOCHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Jia Wang, Hong-Ru Zhai, Si-Fei Ma, Hou-Zhen Shi, Wei-Jun Zhang, Qi Yun, Wen-Jun Liu, Zi-Zhong Liu, Wei-Ning Zhang
Summary: The role of FOXG1 in adult hippocampal neurogenesis is explored in this study. FOXG1 overexpression promotes neuronal lineage progression and contributes to the growth of the precursor cell population. Furthermore, FOXG1 induces the differentiation of radial-glia like neural progenitor cells into neuroblasts and promotes the exit of lineage-committed cells from the cell cycle.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Medicine, Research & Experimental
Hee Ra Park, Heeeun Lee, Won-Kyung Cho, Jin Yeul Ma
Summary: This study found that water extract of Lilii Bulbus (WELB) can improve memory and spatial memory in mice, as well as promote hippocampal neurogenesis, suggesting its potential for treating neurodegenerative diseases.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Article
Multidisciplinary Sciences
Keiyo Takubo, Phyo Wai Htun, Takeshi Ueda, Yasuyuki Sera, Masayuki Iwasaki, Miho Koizumi, Kohei Shiroshita, Hiroshi Kobayashi, Miho Haraguchi, Shintaro Watanuki, Zen-ichiro Honda, Norimasa Yamasaki, Ayako Nakamura- Ishizu, Fumio Arai, Noboru Motoyama, Tomohisa Hatta, Tohru Natsume, Toshio Suda, Hiroaki Honda
Summary: The gene Mbtd1 plays a crucial role in regulating the number and function of hematopoietic stem cells. In mice deficient in Mbtd1, the numbers of stem cells and progenitors increase, and these deficient stem cells exhibit an overactive cell cycle and impaired response to stress. The study also shows that Mbtd1 is directly involved in maintaining cell cycle quiescence by binding to the promoter region of the FoxO3a gene, which is essential for the normal function of hematopoietic stem cells.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Review
Biochemistry & Molecular Biology
Taeko Kobayashi, Ryoichiro Kageyama
Summary: Quiescence is a cellular strategy that maintains somatic stem cells in a low metabolic state in a specific niche to prevent premature exhaustion in the brain. During development, neural stem cells actively proliferate and differentiate, while most adult neural stem cells are quiescent to ensure a sustainable source of stem cells throughout life. The transition between quiescence and proliferation is tightly regulated by various signaling pathways, with recent studies highlighting the role of proteostasis and lysosomes in the quiescence of adult NSCs.
Article
Cell & Tissue Engineering
Ruben Dries, Agata Stryjewska, Kathleen Coddens, Satoshi Okawa, Tineke Notelaers, Judith Birkhoff, Mike Dekker, Catherine M. Verfaillie, Antonio del Sol, Eskeatnaf Mulugeta, Andrea Conidi, Frank G. Grosveld, Danny Huylebroeck
Article
Hematology
Masato Miyata, Nynke Gillemans, Dorit Hockman, Jeroen A. A. Demmers, Jan -Fang Cheng, Jun Hou, Matti Salminen, Christopher A. Fisher, Stephen Taylor, Richard J. Gibbons, Jared J. Ganis, Leonard I. Zon, Frank Grosveld, Eskeatnaf Mulugeta, Tatjana Sauka-Spengler, Douglas R. Higgs, Sjaak Philipsen
Article
Cell Biology
Ilaria Parenti, Farah Diab, Sara Ruiz Gil, Eskeatnaf Mulugeta, Valentina Casa, Riccardo Berutti, Rutger W. W. Brouwer, Valerie Dupe, Juliane Eckhold, Elisabeth Graf, Beatriz Puisac, Feliciano Ramos, Thomas Schwarzmayr, Macarena Moronta Gines, Thomas van Staveren, Wilfred F. J. van IJcken, Tim M. Strom, Juan Pie, Erwan Watrin, Frank J. Kaiser, Kerstin S. Wendt
Article
Cell Biology
Lisanne C. Groeneveldt, Tim Herpelinck, Marina Marechal, Constantinus Politis, Wilfred F. J. van IJcken, Danny Huylebroeck, Liesbet Geris, Eskeatnaf Mulugeta, Frank P. Luyten
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2020)
Article
Cell & Tissue Engineering
Lachlan Harris, Piero Rigo, Thomas Stiehl, Zachary B. Gaber, Sophie H. L. Austin, Maria del Mar Masdeu, Amelia Edwards, Noelia Urban, Anna Marciniak-Czochra, Francois Guillemot
Summary: The stabilization of neural stem cell numbers in young adults is a result of coordinated changes in stem cell behavior, with resting cells having a higher activation rate and greater contribution to neurogenesis. This change is associated with a progressive reduction in expression of the pro-activation protein ASCL1 due to increased post-translational degradation, providing insights into hippocampal NSC dynamics and neurogenesis rates in different mammalian species, including humans.
Review
Developmental Biology
Noelia Urban, Tom H. Cheung
Summary: Quiescence is a cellular state where a cell is out of the cell cycle but can still divide, and adult stem cells' ability to maintain quiescence is vital for tissue homeostasis and regeneration. Recent studies show that quiescence is an actively regulated process, and adult stem cells are sensitive to external stimuli. This has raised hopes of enhancing the reactivation potential of adult stem cells for improving tissue function during aging.
Article
Cardiac & Cardiovascular Systems
Willeke de Haan, Wouter Dheedene, Katerina Apelt, Sofiane Decombas-Deschamps, Stefan Vinckier, Stefaan Verhulst, Andrea Conidi, Thomas Deffieux, Michael W. Staring, Petra Vandervoort, Ellen Caluwe, Marleen Lox, Inge Mannaerts, Tsuyoshi Takagi, Joris Jaekers, Geert Berx, Jody Haigh, Baki Topal, An Zwijsen, Yujiro Higashi, Leo A. van Grunsven, Wilfred F. J. van IJcken, Eskeatnaf Mulugeta, Mickael Tanter, Franck P. G. Lebrin, Danny Huylebroeck, Aernout Luttun
Summary: This study demonstrated the specific role of Zeb2 in liver endothelial cells, showing that Zeb2 deficiency leads to significant changes in gene expression related to angiogenesis and potential promotion of endothelial dedifferentiation. Furthermore, Zeb2 regulates LSEC-HSC communication and HSC activation to maintain vascular structure and inhibit liver fibrosis.
CARDIOVASCULAR RESEARCH
(2022)
Article
Developmental Biology
Sophie H. L. Austin, Rut Gabarro-Solanas, Piero Rigo, Oana Paun, Lachlan Harris, Francois Guillemot, Noelia Urban
Summary: Adult mouse hippocampal neural stem cells respond to Wnt/beta-catenin signaling, which can induce neuronal differentiation and activate or differentiate quiescent NSCs in a dose-dependent manner. However, the deletion of beta-catenin in NSCs does not affect their activation or maintenance of stem cell characteristics.
Article
Neurosciences
Noelia Urban
Summary: This article discusses the regulation of quiescence in adult neural stem cells (aNSCs) and proposes a dynamic transition model that acknowledges the complexity of aNSC states. The author emphasizes the need for a model that goes beyond simple activation transition in order to better understand the control of aNSC activity and neurogenesis.
FRONTIERS IN NEUROSCIENCE
(2022)
Article
Biology
Lucile Marion-Poll, Jean-Pierre Roussarie, Lieng Taing, Cloelia Dard-Dascot, Nicolas Servant, Yan Jaszczyszyn, Emmanuelle Jordi, Eskeatnaf Mulugeta, Denis Herve, Deborah Bourc'his, Paul Greengard, Claude Thermes, Jean-Antoine Girault
Summary: Neuronal DNA modifications, including methylation outside CpG context and abundant 5-hydroxymethylation, show distinct patterns in striatal projection neurons expressing D1 or D2 dopamine receptors. DNA methylation differences mainly occur in large genomic clusters related to differentially expressed genes, while hydroxymethylation differences are scattered and affect transcription factor binding sites.
COMMUNICATIONS BIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Ruben Boers, Joachim Boers, Beatrice Tan, Marieke E. van Leeuwen, Evelyne Wassenaar, Erlantz Gonzalez Sanchez, Esther Sleddens, Yasha Tenhagen, Eskeatnaf Mulugeta, Joop Laven, Menno Creyghton, Willy Baarends, Wilfred F. J. van IJcken, Joost Gribnau
Summary: In this study, a bacterial methylation mark was used to label transcribed genes and active enhancers, allowing for tracking of cell state changes. This technique revealed rapid and coordinated activation of enhancers and nearby genes during enterocyte differentiation in the intestine. Furthermore, it provided insights into the chromatin landscape of intestinal stem cells and their differentiation process.
NATURE BIOTECHNOLOGY
(2023)
Article
Microbiology
Brenda Rocamonde, Sandrine Alais, Rodolphe Pelissier, Valerie Moulin, Brigitte Rimbaud, Romain Lacoste, Noemie Aurine, Camille Baquerre, Bertrand Pain, Yuetsu Tanaka, Cyrille Mathieu, Helene Dutartre
Summary: The human T-cell leukemia virus (HTLV)-1 is responsible for an aggressive neurodegenerative disease (HAM/TSP) and multiple neurological alterations. The ability of HTLV-1 to infect cells in the central nervous system (CNS) and the resulting neuroimmune response have not been well-established. Using human induced pluripotent stem cells (hiPSC) and naturally infected nonhuman primates (NHP) as models, neuronal cells derived from hiPSC differentiation in neural polycultures were identified as the primary cell population infected by HTLV-1. Additionally, neuronal infection with STLV-1 was observed in spinal cord regions, as well as in brain cortical and cerebellar sections of postmortem NHP. Reactive microglial cells were also found in the infected areas, suggesting an immune antiviral response. These findings highlight the importance of developing more efficient models to understand HTLV-1 neuroinfection and propose an alternative mechanism for HAM/TSP.
Article
Biology
Lucie Kulhankova, Diego Montiel Gonzalez, Eric Bindels, Daniel Kling, Manfred Kayser, Eskeatnaf Mulugeta
Summary: A method combining scRNA-sequencing and a bioinformatics pipeline was developed to separate individuals in multi-person biological mixtures and genetically identify them. This method showed success in separating individuals and determining their ancestry and sex in both de-novo generated and in-silico mixtures. The approach demonstrated the feasibility of single-cell approaches in deconvoluting biological mixtures and individually identifying the contributors, with potential application in crime scene investigation.
COMMUNICATIONS BIOLOGY
(2023)
