Article
Oncology
Jonathan A. Chacon-Barahona, Jeffrey P. MacKeigan, Nathan J. Lanning
Summary: In this study, we examined the viability of cancer cells under different metabolic conditions. We found that the pentose phosphate pathway is essential for survival under glycolytic conditions, while mTOR signaling is essential for survival under oxidative phosphorylation conditions. Additionally, we identified similarities and differences in the gene expression profiles of glycolytic and non-glycolytic tumors, supporting the classification of tumors based on metabolic phenotype.
Article
Biochemistry & Molecular Biology
Mengru Liu, Xinyu Zhou, Yue Li, Shijia Ma, Ling Pan, Xingxian Zhang, Wanqing Zheng, Zhanxun Wu, Ke Wang, Anil Ahsan, Jiaying Wu, Lei Jiang, Yangyang Lu, Weiwei Hu, Zhenghong Qin, Zhong Chen, Xiangnan Zhang
Summary: TIGAR induces autophagy to alleviate oxidative stress and activate Nrf2 in prolonged ischemic brains, providing sustainable antioxidant defense.
Review
Cell Biology
Chelsea Schiliro, Bonnie L. Firestein
Summary: Cancer cells alter metabolic processes to sustain their rapid growth, including shifting from oxidative phosphorylation to aerobic glycolysis, and increasing other pathways to generate necessary molecules.
Article
Biochemistry & Molecular Biology
Theodora Panagaki, Laszlo Pecze, Elisa B. Randi, Anni I. Nieminen, Csaba Szabo
Summary: Increased H2S generation in Down syndrome (DS) promotes pseudohypoxia and contributes to cellular metabolic dysfunction by causing a shift from oxidative phosphorylation to glycolysis.
Article
Biochemistry & Molecular Biology
Rosaura Rodicio, Hans-Peter Schmitz, Jurgen J. Heinisch
Summary: The milk yeast Kluyveromyces lactis degrades glucose through glycolysis and the pentose phosphate pathway and regulates gluconeogenesis. The enzymes fructose-1,6-bisphosphate aldolase (FBA) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) play important roles in these metabolic processes. Among the GAPDH isoforms, KlTdh2 is the major player in glucose metabolism and may have additional functions in gene expression regulation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Orthopedics
X. Wu, C. Liyanage, M. Plan, T. Stark, T. McCubbin, R. A. Barrero, J. Batra, R. Crawford, Y. Xiao, I. Prasadam
Summary: This study aims to identify the changes in energy metabolism in osteoarthritis (OA) cartilage. Metabolomics, proteomics, and transcriptomics analyses were conducted to investigate the global alterations in OA metabolism. The results showed increased lactate production and decreased mitochondrial respiratory rates in OA chondrocytes. This study provides a basis for targeting metabolism as a therapeutic approach for OA.
OSTEOARTHRITIS AND CARTILAGE
(2023)
Review
Endocrinology & Metabolism
Tomas Duraj, Josefa Carrion-Navarro, Thomas N. Seyfried, Noemi Garcia-Romero, Angel Ayuso-Sacido
Summary: Aberrant cancer metabolism, characterized by a preference for various metabolites including aerobic glycolysis and glutaminolysis, challenges the traditional static metabolic profile. Metabolic plasticity, often overlooked in therapeutic strategies, is crucial for understanding cancer bioenergetics. Standardization of metabolic readouts can provide a comprehensive metabolic picture in cancer research.
MOLECULAR METABOLISM
(2021)
Article
Physiology
Mathews Valuparampil Varghese, Joel James, Olga Rafikova, Ruslan Rafikov
Summary: G6PD deficiency in mice leads to spontaneous pulmonary hypertension and right heart remodeling, along with increased oxidative stress and metabolic reprogramming. The deficiency activates collateral pathways at the cost of increased oxidative stress, contributing to the PH phenotype.
AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY
(2021)
Article
Nutrition & Dietetics
Semira R. Ortiz, Alexander Heinz, Karsten Hiller, Martha S. Field
Summary: Erythritol synthesis in human lung carcinoma cells is proportional to flux through the pentose phosphate pathway (PPP) and regulated by non-oxidative PPP enzymes.
FRONTIERS IN NUTRITION
(2022)
Article
Geriatrics & Gerontology
Xiaoxin Wu, Xiwei Fan, Ross Crawford, Yin Xiao, Indira Prasadam
Summary: The function of articular cartilage relies on the coordinated metabolic regulation in chondrocytes. Dysregulation of cellular metabolism can lead to degenerative diseases like osteoarthritis. Chondrocytes in cartilage have limited access to nutrients and oxygen, as well as pathways for metabolite removal. Central carbon metabolism pathways are crucial for maintaining cartilage homeostasis. Increased understanding of cellular metabolism and its dysregulation in osteoarthritis can provide insights into potential therapeutic opportunities.
Article
Biochemistry & Molecular Biology
Yi Zhang, Lei Xu, Zeqiang Ren, Xin Liu, Jun Song, Pengbo Zhang, Chong Zhang, Shuai Gong, Nai Wu, Xiuzhong Zhang, Chanbin Xie, Zhixing Lu, Min Ma, Yi Zhang, Yifei Chen, Changwei Lin
Summary: A lncRNA called LINC01615 was discovered to be upregulated in colorectal cancer (CRC) cells in response to serum starvation. LINC01615 promotes CRC cell survival under serum-deprived conditions by activating the pentose phosphate pathway (PPP) and enhancing nucleotide and lipid synthesis. It achieves this by promoting G6PD expression and preventing METTL3 degradation.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2023)
Article
Medicine, Research & Experimental
Iart Luca Shytaj, Francesco Andrea Procopio, Mohammad Tarek, Irene Carlon-Andres, Hsin-Yao Tang, Aaron R. Goldman, MohamedHusen Munshi, Virender Kumar Pal, Mattia Forcato, Sheetal Sreeram, Konstantin Leskov, Fengchun Ye, Bojana Lucic, Nicolly Cruz, Lishomwa C. Ndhlovu, Silvio Bicciato, Sergi Padilla-Parra, Ricardo Sobhie Diaz, Amit Singh, Marina Lusic, Jonathan Karn, David Alvarez-Carbonell, Andrea Savarino
Summary: The study reveals that latent HIV-1 infection downregulates glycolysis and relies on the pentose phosphate pathway for maintaining latency. Reactivation of the virus can revert this effect and decrease proviral DNA in infected cells.
EMBO MOLECULAR MEDICINE
(2021)
Review
Biochemistry & Molecular Biology
Joanna Kubik, Ewelina Humeniuk, Grzegorz Adamczuk, Barbara Madej-Czerwonka, Agnieszka Korga-Plewko
Summary: Cancer, the second leading cause of global death, necessitates the development of new treatment strategies that are highly effective and have few side effects. This review presents the changes in metabolic pathways in cancer cells and highlights the differences in metabolic phenotype between cancer cells and normal cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Sara Franceschi, Francesca Lessi, Mariangela Morelli, Michele Menicagli, Francesco Pasqualetti, Paolo Aretini, Chiara Maria Mazzanti
Summary: This study highlights the importance of SHPK in glioblastoma for tumor progression, and proposes this enzyme and the nonoxidative pentose phosphate pathway as possible therapeutic targets.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cardiac & Cardiovascular Systems
Abdelrahman A. Zaied, Masuko Ushio-Fukai, Tohru Fukai, Anita Kovacs-Kasa, Suhib Alhusban, Varadarajan Sudhahar, Vijay C. C. Ganta, Brian H. H. Annex
Summary: In endothelial cells, PFKFB3 plays a crucial role in regulating glycolysis, which is the main pathway for ATP generation. Both VEGF(165)a and microRNA-93 promote angiogenesis. In peripheral artery disease models, microRNA-93 treatment improves perfusion recovery, while VEGF(165)a treatment does not. This suggests that activating the glycolysis pathway to enhance angiogenesis may have therapeutic advantages.
JOURNAL OF THE AMERICAN HEART ASSOCIATION
(2023)
Review
Pharmacology & Pharmacy
Takeshi Susukida, Shigeki Aoki, Tomohiro Shirayanagi, Yushiro Yamada, Saki Kuwahara, Kousei Ito
DRUG METABOLISM REVIEWS
(2020)
Article
Immunology
Yuying Gao, Binbin Song, Shigeki Aoki, Kousei Ito
Summary: The interaction between HLA-B*57:01 and the FLX-albumin conjugate may be an important risk factor for FLX-induced liver injury, and patients carrying HLA-B*57:01 may be more susceptible to its effects. The sensitivity of the immune system does not play a decisive role in FLX-induced liver injury.
IMMUNOLOGY LETTERS
(2022)
Article
Toxicology
Takumi Nukaga, Akinori Takemura, Yuka Endo, Yoshihiro Uesawa, Kousei Ito
Summary: Drug-induced liver injury (DILI) is a major concern in drug development and an accurate assessment of toxicity at the preclinical stage is necessary. In this study, a model was constructed using molecular initiating events (MIE), admetSAR parameters, and clinical information to predict DILI risk for various compounds. The accuracy of the model was improved by incorporating multiple parameters and MIE was found to be a valuable parameter for detecting high DILI risk compounds in the early stages of drug development. Furthermore, the effect of changes in maximum daily dose (MDD) on DILI risk was examined to estimate the maximum safety dose (MSD) for clinical use and prevent underestimation of DILI risk.
TOXICOLOGY RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Soojung Hahn, Gyuri Kim, Sang-Man Jin, Jae Hyeon Kim
Summary: This study utilized three-dimensional intestinal organoids to investigate the effects of metformin on inflammatory bowel disease (IBD) and found that metformin can enhance intestinal barrier function and reduce levels of inflammatory cytokines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
V. V. Sudarev, M. S. Gette, S. V. Bazhenov, O. M. Tilinova, E. V. Zinovev, I. V. Manukhov, A. I. Kuklin, Yu. L. Ryzhykau, A. V. Vlasov
Summary: This study investigated the self-assembly processes of ferritin-based protein complexes and obtained structurally characterized oligomeric states. These results provide new potential and opportunities for the application of ferritin in various fields.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Yalda Sabaghi, Farnaz Pourfarzad, Leila Zolghadr, Azita Bahrami, Tahereh Shojazadeh, Alireza Farasat, Nematollah Gheibi
Summary: p-Coumaric acid (p-CA) is a plant compound with anti-cancer activities. This study designed a nano-liposomal carrier containing p-CA to enhance its effectiveness against melanoma cells. The findings showed that the liposomal form of p-CA had a greater impact on the cells. Kinetic modeling indicated that the best fitting model was zero-order.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
M. D. Nazmul Hasan, Md Mahfuzur Rahman, Al Asmaul Husna, Nobuhiro Nozaki, Osamu Yamato, Naoki Miura
Summary: This study investigated the expression of ncRNAs other than miRNAs in different histologic subtypes of canine mammary gland tumors (MGT). Three aberrantly expressed ncRNAs were identified as potential biomarkers for differentiating MGT subtypes. YRNA and tRFs expression levels were found to be decreased in metastatic compared to primary MGT cell lines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Seine A. Shintani
Summary: In this study, the researchers used signal analysis to study the instantaneous amplitude and phase of sarcomeric oscillations in skeletal muscle. They identified two types of oscillations, sarcomeric oscillations and sarcosynced oscillations, and visualized their behavior during propagating waves. The researchers discovered the presence of sarcomeric defect holes and sarcomeric collision holes, which are important indicators for understanding the oscillation properties of sarcomeres. This finding has important implications for improving our understanding of muscle function and its regulatory mechanisms.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Shuanglin Zhang, Yuzhong Jia, Guolan Ma, Yanyan Yang, Zhenzhen Cao, Antao Luo, Zefu Zhang, Shihan Li, Jie Wen, Hanfeng Liu, Jihua Ma
Summary: Bupleurum is an antiarrhythmic agent that may exert its effects by inhibiting L-type calcium channels.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Tomotaka Ohkubo, Yasuhiko Matsumoto, Hiroaki Sasaki, Kaoru Kinoshita, Yuki Ogasawara, Takashi Sugita
Summary: This study found that Citrobacter koseri inhibits the growth of Staphylococcus epidermidis, disrupting the balance between S. epidermidis and Staphylococcus aureus, and exacerbating inflammation in atopic dermatitis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Toshifumi Asano, Philipp Sasse, Takao Nakata
Summary: A Cre recombination-based fluorescent reporter system was developed to monitor cell-cell fusion. The system successfully detected the formation of multinuclear myotubes and placental syncytiotrophoblast. This tool could facilitate the study of cell-to-cell fusion.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Ke Shi, Yunlong Shan, Xiao Sun, Kuida Chen, Qiong Luo, Qiang Xu
Summary: This study found that low expression of TP53INP2 is associated with poor survival in colorectal cancer (CRC) patients. As the malignancy of CRC progresses, TP53INP2 expression gradually decreases. Knockdown of TP53INP2 promotes CRC cell proliferation and tumor growth. Mechanistically, TP53INP2 deficiency decreases phosphorylation of beta-catenin, leading to increased accumulation and enhanced nuclear translocation and transcriptional activity. Additionally, TP53INP2 sequesters TIM50, inhibiting its activation of beta-catenin. In conclusion, downregulation of TP53INP2 promotes CRC progression by activating beta-catenin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Martina Rossi, Fabio Tomaselli, Alejandro Hochkoeppler
Summary: Oligomeric enzymes are known for their higher catalytic rates compared to monomeric enzymes, but the extent of additivity in their activity is still not well understood. This study used tetrameric rabbit lactate dehydrogenase as a model to examine the kinetics of its catalytic action. Surprisingly, when the concentration of the limiting reactant exceeded that of a single subunit, there was a significant slowdown in the enzyme's conformational rearrangements.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Amin Sahraei, Mohammad Javad Shamsoddini, Fakhrossadat Mohammadi, Leila Hassani
Summary: This study explored the inhibitory effects of gallium curcumin, indium curcumin, and vanadyl curcumin on the amyloid fibrillation of hen egg white lysozyme, as well as the binding interactions of these metal complexes with the enzyme. The results showed that indium curcumin and vanadyl curcumin exhibited higher binding affinities and stronger inhibitory effects on amyloid fibrillation compared to gallium curcumin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Takahiro Sasaki, Yoshiki Kuse, Shinsuke Nakamura, Masamitsu Shimazawa
Summary: PGRN deficiency plays a significant role in cardiac remodeling and arrhythmias post-myocardial infarction (MI), potentially by promoting metabolic abnormalities in macrophages.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Hongwei Zhao, Yiqiang Li, Yibo Zhang, Chi Zhang
Summary: Electrical brain stimulation technology is commonly used to treat brain neurological disorders, but it can cause side effects. This study investigated the impact of electric fields on nerve fibers and revealed the possible origin of side effects. The findings provide guidance for selecting electrical parameters in clinical stimulation therapy.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Julia S. Scott, Lake-Ee Quek, Andrew J. Hoy, Johannes V. Swinnen, Zeyad D. Nassar, Lisa M. Butler
Summary: The fatty acid elongation enzyme ELOVL5 plays a critical role in promoting metastasis in prostate cancer. Knocking down ELOVL5 leads to the accumulation of malonyl-CoA, which inhibits fatty acid oxidation in mitochondria. This study highlights the importance of fatty acid elongation in regulating cell viability and provides a potential target for prostate cancer treatment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Zan Zhou, Wen-jun Jiang, Li Li, Jun-qiang Si
Summary: This study investigates the effect of noise exposure on cognitive function in mice and explores the underlying molecular mechanisms. The findings suggest that noise exposure leads to increased inflammation, increased phosphorylation of Tau protein, and decreased levels of postsynaptic density protein, resulting in cognitive impairment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)