Article
Biochemistry & Molecular Biology
Jia Shi, Riku Kanoya, Yurina Tani, Sodai Ishikawa, Rino Maeda, Sana Suzuki, Fumiya Kawanami, Naoko Miyagawa, Katsuhiko Takahashi, Teruaki Oku, Ami Yamamoto, Kaori Fukuzawa, Motowo Nakajima, Tatsuro Irimura, Nobuaki Higashi
Summary: This study found that sulfated hyaluronan can inhibit the enzymatic and biological actions of heparanase, a key enzyme involved in cancer malignancy and inflammation. Sulfated hyaluronan effectively inhibits the degradation of heparan sulfate by human and mouse heparanase, and it can bind to heparanase molecules. In the presence of sulfated hyaluronan, the invasion of heparanase-positive cells and the release of CCL2 are significantly suppressed. The inhibitory effect is likely due to competitive binding between sulfated hyaluronan and heparanase molecules.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Casper de Boer, Zachary Armstrong, Vincent A. J. Lit, Uri Barash, Gijs Ruijgrok, Ilanit Boyango, Merle M. Weitzenberg, Sybrin P. Schroder, Alexi J. C. Sarris, Nico J. Meeuwenoord, Pedro Bule, Yasmine Kayal, Neta Ilan, Jeroen D. C. Codee, Israel Vlodavsky, Herman S. Overkleeft, Gideon J. Davies, Liang Wu
Summary: Research has shown that by developing effective HPSE inhibitors, extracellular HPSE activity can be reduced, thereby reducing the aggressiveness of cancer and potentially controlling pathological HPSE-driven malignancies.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Oncology
Miriam Gross-Cohen, Sari Feld, Gil Arvatz, Neta Ilan, Israel Vlodavsky
Summary: Hpa2, a close homolog of heparanase, plays a role in a rare genetic disease called urofacial syndrome, indicating its importance in protein coding; despite lacking HS-degrading activity, it shows a high affinity to HS; experimental evidence shows that Hpa2 affects cell adhesion, migration, and colony morphology.
FRONTIERS IN ONCOLOGY
(2021)
Article
Hematology
Raghuram Koganti, Abdullah Memon, Deepak Shukla
Summary: Heparan sulfate is a glycosaminoglycan found in mammalian tissues that contributes to the structural integrity of the extracellular matrix and cell signaling, but is also exploited by viruses during infection. Loss or inhibition of heparan sulfate proteoglycans during infection can lead to significant decreases in viral entry and infectivity, affecting multiple viruses such as herpesviruses, HIV, dengue virus, HPV, and coronaviruses.
SEMINARS IN THROMBOSIS AND HEMOSTASIS
(2021)
Article
Chemistry, Multidisciplinary
Joseph Wakpal, Vishaka Pathiranage, Alice R. Walker, Hien M. Nguyen
Summary: This article describes a method for synthesizing a library of HS mimetics from natural aminoglycosides, reducing the number of steps compared to traditional methods. Computational modeling was used to identify a new class of trisaccharide compounds derived from tobramycin that mimic natural HS and have a strong binding to heparanase but a low affinity for off-target platelet factor-4 protein.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Chemistry, Multidisciplinary
Mohit Chhabra, Gareth G. Doherty, Nicholas W. See, Neha S. Gandhi, Vito Ferro
Summary: Heparan sulfate is a complex polysaccharide widely expressed on cell surfaces and in the extracellular matrix, interacting with numerous proteins to mediate various biological and pathological processes. Developing synthetically tractable HS mimetics with fewer side effects has become an attractive approach for treating cancer and infectious diseases.
Article
Biochemistry & Molecular Biology
Carina Mucciolo Melo, Helena Bonciani Nader, Giselle Zenker Justo, Maria Aparecida Silva Pinhal
Summary: Heparanase is an enzyme in mammals that cleaves heparan sulfate/heparin chains from proteoglycans. Experiments showed that exogenous heparin can increase Heparanase expression through the Wnt/beta-catenin pathway.
MOLECULAR BIOLOGY REPORTS
(2021)
Article
Medicine, General & Internal
Elisabeth Zechendorf, Katharina Schroeder, Lara Stiehler, Nadine Frank, Christian Beckers, Sandra Kraemer, Michael Dreher, Alexander Kersten, Christoph Thiemermann, Gernot Marx, Tim-Philipp Simon, Lukas Martin
Summary: Heparanase activity and heparan sulfate levels correlate with the severity and outcomes of COVID-19, and may be helpful in predicting clinical progression and outcomes in patients.
JOURNAL OF CLINICAL MEDICINE
(2022)
Review
Biochemistry & Molecular Biology
Alyce J. Mayfosh, Tien K. Nguyen, Mark D. Hulett
Summary: The extracellular matrix plays crucial roles in maintaining tissue structure, immune defense, and acting as a reservoir for bioactive molecules, with heparan sulfate proteoglycans (HSPGs) and their component heparan sulfate being essential in these processes. Understanding the regulation of heparanase and its downstream targets is important for potential therapeutic strategies in inflammatory diseases, atherosclerosis, fibrosis, and cancer metastasis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Multidisciplinary
Mohit Chhabra, Jennifer C. Wilson, Liang Wu, Gideon J. Davies, Neha S. Gandhi, Vito Ferro
Summary: This study investigates the interaction between Pixatimod and heparanase using a combination of NMR experiments and molecular modeling. The results show that Pixatimod can bind to the active site of heparanase in different conformations, effectively blocking the binding of its natural substrate. This study provides insights for the design of next-generation heparanase inhibitors and substrates.
CHEMISTRY-A EUROPEAN JOURNAL
(2022)
Review
Cell Biology
Tien K. Nguyen, Stephanie Paone, Enoch Chan, Ivan K. H. Poon, Amy A. Baxter, Shane R. Thomas, Mark D. Hulett
Summary: Cardiovascular disease is the leading cause of death and disability worldwide, and atherosclerosis plays a crucial role in its development. Heparanase, as the only mammalian enzyme known to cleave heparan sulfate, has been found to be involved in various physiological and pathological processes, including inflammation and plaque formation. This review focuses on the emerging role of heparanase in atherosclerosis and provides an up-to-date overview of its functions.
Article
Immunology
Kari Vaahtomeri, Christine Moussion, Robert Hauschild, Michael Sixt
Summary: Chemokine CCL21, necessary for dendritic cell migration, does not require heparan sulfates on lymphatic endothelial cells for gradient formation.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Alexandra De Zutter, Luna Dillemans, Nele Berghmans, Sam Noppen, Helena Crijns, Paulien Verscheure, Janne Verhaegen, Erik Martens, Lotte Vanbrabant, Noemie Portner, Dominique Schols, Paul Proost, Sofie Struyf
Summary: Glycosaminoglycans/proteoglycans (GAGs/PGs) play a crucial role in regulating tumor progression and can be potential targets for anti-cancer therapy. A D-amino acid version of CXCL9(74-103), called D-CXCL9(74-103), showed anti-angiogenic properties and inhibited endothelial spheroid sprouting and vessel formation. D-CXCL9(74-103) also exhibited anti-tumoral activity against heparan sulfate high B16-BL6 melanoma cells but had no effect on chondroitin sulfate high HCT116 colon carcinoma cells. These findings highlight the importance of tumor-associated GAGs in adhesion and metastasis and suggest the potential of D-CXCL9(74-103) as a therapeutic agent for heparan sulfate high tumors.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Neurosciences
Xiao Zhang, Paul O'Callaghan, Honglian Li, Yingxia Tan, Ganlin Zhang, Uri Barash, Xiaomin Wang, Lars Lannfelt, Israel Vlodavsky, Ulf Lindahl, Jin-Ping Li
Summary: Defective clearance of amyloid-beta in the brain, caused by elevated levels of heparanase, results in impaired perivascular drainage and accumulation of A beta, contributing to the pathogenesis of Alzheimer's disease. This study highlights the potential role of heparanase in altering the blood-brain barrier and promoting amyloid-beta accumulation in the brain.
ACTA NEUROPATHOLOGICA COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Zuopeng Wu, Rebecca A. Sweet, Gerard F. Hoyne, Charmaine J. Simeonovic, Christopher R. Parish
Summary: It has been accepted for decades that T lymphocytes and metastasising tumour cells traverse basement membranes (BM) by deploying a battery of degradative enzymes, particularly proteases. However, recent studies suggest that there are other mechanisms that allow cell migration through basement membranes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Soojung Hahn, Gyuri Kim, Sang-Man Jin, Jae Hyeon Kim
Summary: This study utilized three-dimensional intestinal organoids to investigate the effects of metformin on inflammatory bowel disease (IBD) and found that metformin can enhance intestinal barrier function and reduce levels of inflammatory cytokines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
V. V. Sudarev, M. S. Gette, S. V. Bazhenov, O. M. Tilinova, E. V. Zinovev, I. V. Manukhov, A. I. Kuklin, Yu. L. Ryzhykau, A. V. Vlasov
Summary: This study investigated the self-assembly processes of ferritin-based protein complexes and obtained structurally characterized oligomeric states. These results provide new potential and opportunities for the application of ferritin in various fields.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Yalda Sabaghi, Farnaz Pourfarzad, Leila Zolghadr, Azita Bahrami, Tahereh Shojazadeh, Alireza Farasat, Nematollah Gheibi
Summary: p-Coumaric acid (p-CA) is a plant compound with anti-cancer activities. This study designed a nano-liposomal carrier containing p-CA to enhance its effectiveness against melanoma cells. The findings showed that the liposomal form of p-CA had a greater impact on the cells. Kinetic modeling indicated that the best fitting model was zero-order.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
M. D. Nazmul Hasan, Md Mahfuzur Rahman, Al Asmaul Husna, Nobuhiro Nozaki, Osamu Yamato, Naoki Miura
Summary: This study investigated the expression of ncRNAs other than miRNAs in different histologic subtypes of canine mammary gland tumors (MGT). Three aberrantly expressed ncRNAs were identified as potential biomarkers for differentiating MGT subtypes. YRNA and tRFs expression levels were found to be decreased in metastatic compared to primary MGT cell lines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Seine A. Shintani
Summary: In this study, the researchers used signal analysis to study the instantaneous amplitude and phase of sarcomeric oscillations in skeletal muscle. They identified two types of oscillations, sarcomeric oscillations and sarcosynced oscillations, and visualized their behavior during propagating waves. The researchers discovered the presence of sarcomeric defect holes and sarcomeric collision holes, which are important indicators for understanding the oscillation properties of sarcomeres. This finding has important implications for improving our understanding of muscle function and its regulatory mechanisms.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Shuanglin Zhang, Yuzhong Jia, Guolan Ma, Yanyan Yang, Zhenzhen Cao, Antao Luo, Zefu Zhang, Shihan Li, Jie Wen, Hanfeng Liu, Jihua Ma
Summary: Bupleurum is an antiarrhythmic agent that may exert its effects by inhibiting L-type calcium channels.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Tomotaka Ohkubo, Yasuhiko Matsumoto, Hiroaki Sasaki, Kaoru Kinoshita, Yuki Ogasawara, Takashi Sugita
Summary: This study found that Citrobacter koseri inhibits the growth of Staphylococcus epidermidis, disrupting the balance between S. epidermidis and Staphylococcus aureus, and exacerbating inflammation in atopic dermatitis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Toshifumi Asano, Philipp Sasse, Takao Nakata
Summary: A Cre recombination-based fluorescent reporter system was developed to monitor cell-cell fusion. The system successfully detected the formation of multinuclear myotubes and placental syncytiotrophoblast. This tool could facilitate the study of cell-to-cell fusion.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Ke Shi, Yunlong Shan, Xiao Sun, Kuida Chen, Qiong Luo, Qiang Xu
Summary: This study found that low expression of TP53INP2 is associated with poor survival in colorectal cancer (CRC) patients. As the malignancy of CRC progresses, TP53INP2 expression gradually decreases. Knockdown of TP53INP2 promotes CRC cell proliferation and tumor growth. Mechanistically, TP53INP2 deficiency decreases phosphorylation of beta-catenin, leading to increased accumulation and enhanced nuclear translocation and transcriptional activity. Additionally, TP53INP2 sequesters TIM50, inhibiting its activation of beta-catenin. In conclusion, downregulation of TP53INP2 promotes CRC progression by activating beta-catenin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Martina Rossi, Fabio Tomaselli, Alejandro Hochkoeppler
Summary: Oligomeric enzymes are known for their higher catalytic rates compared to monomeric enzymes, but the extent of additivity in their activity is still not well understood. This study used tetrameric rabbit lactate dehydrogenase as a model to examine the kinetics of its catalytic action. Surprisingly, when the concentration of the limiting reactant exceeded that of a single subunit, there was a significant slowdown in the enzyme's conformational rearrangements.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Amin Sahraei, Mohammad Javad Shamsoddini, Fakhrossadat Mohammadi, Leila Hassani
Summary: This study explored the inhibitory effects of gallium curcumin, indium curcumin, and vanadyl curcumin on the amyloid fibrillation of hen egg white lysozyme, as well as the binding interactions of these metal complexes with the enzyme. The results showed that indium curcumin and vanadyl curcumin exhibited higher binding affinities and stronger inhibitory effects on amyloid fibrillation compared to gallium curcumin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Takahiro Sasaki, Yoshiki Kuse, Shinsuke Nakamura, Masamitsu Shimazawa
Summary: PGRN deficiency plays a significant role in cardiac remodeling and arrhythmias post-myocardial infarction (MI), potentially by promoting metabolic abnormalities in macrophages.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Hongwei Zhao, Yiqiang Li, Yibo Zhang, Chi Zhang
Summary: Electrical brain stimulation technology is commonly used to treat brain neurological disorders, but it can cause side effects. This study investigated the impact of electric fields on nerve fibers and revealed the possible origin of side effects. The findings provide guidance for selecting electrical parameters in clinical stimulation therapy.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Julia S. Scott, Lake-Ee Quek, Andrew J. Hoy, Johannes V. Swinnen, Zeyad D. Nassar, Lisa M. Butler
Summary: The fatty acid elongation enzyme ELOVL5 plays a critical role in promoting metastasis in prostate cancer. Knocking down ELOVL5 leads to the accumulation of malonyl-CoA, which inhibits fatty acid oxidation in mitochondria. This study highlights the importance of fatty acid elongation in regulating cell viability and provides a potential target for prostate cancer treatment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Zan Zhou, Wen-jun Jiang, Li Li, Jun-qiang Si
Summary: This study investigates the effect of noise exposure on cognitive function in mice and explores the underlying molecular mechanisms. The findings suggest that noise exposure leads to increased inflammation, increased phosphorylation of Tau protein, and decreased levels of postsynaptic density protein, resulting in cognitive impairment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)