Article
Biochemistry & Molecular Biology
D. S. H. Ramklowan, C. Snyman, M. van de Vyver, C. U. Niesler
Summary: Fibroblasts play a crucial role in wound healing by migrating to the injury site and differentiating into myofibroblasts. Impaired wound healing is associated with a lack of myofibroblasts, resulting in reduced tissue strength and function. Communication between fibroblasts and myofibroblasts, as well as other cellular mediators of repair, influences the outcome of wound repair. However, establishing an in vitro model to investigate this communication is challenging. This study presents a model that allows the retention and culture of fibroblast and myofibroblast phenotypes, and demonstrates the importance of considering paracrine communication during wound healing.
Article
Chemistry, Multidisciplinary
Dania Grant-Serroukh, Morag R. Hunter, Ruhina Maeshima, Aristides D. Tagalakis, Ahmad M. Aldossary, Nour Allahham, Gareth R. Williams, Mark Edbrooke, Arpan Desai, Stephen L. Hart
Summary: Despite recent advances in mRNA therapy, the lack of safe and effective delivery vehicles remains a major challenge. In this study, lipid-peptide nanocomplexes were systematically optimized for mRNA delivery. The optimized formulation showed improved transfection efficiency in vitro and in vivo, and retained stable physicochemical properties for a prolonged period of time. This lipid-peptide nanocomplex provides a promising platform for the delivery of therapeutic mRNA.
JOURNAL OF CONTROLLED RELEASE
(2022)
Article
Multidisciplinary Sciences
Jeongmin Ha, Bum Suk Kim, Byungkuk Min, Juhyeon Nam, Jae-Geun Lee, Minhyung Lee, Byoung-Ha Yoon, Yoon Ha Choi, Ilkyun Im, Jung Sun Park, Hyosun Choi, Areum Baek, Sang Mi Cho, Mi-Ok Lee, Ki-Hoan Nam, Ji Young Mun, Mirang Kim, Seon-Young Kim, Mi Young Son, Yong-Kook Kang, Jeong-Soo Lee, Jong Kyoung Kim, Janghwan Kim
Summary: The cytoskeletal linker protein desmoplakin (Dsp) plays a crucial role in both in vitro cellular reprogramming and tissue regeneration. Knockdown of Dsp impairs the formation of intermediate cells during cellular reprogramming and tissue regeneration. This study suggests a potential evolutionary link mediated by Dsp between cellular reprogramming in mammals and tissue regeneration in lower vertebrates, and the intermediate cells may provide alternative approaches for mammalian regenerative therapy.
Review
Biochemistry & Molecular Biology
Aline Yen Ling Wang
Summary: Modified mRNA (modRNA)-based somatic reprogramming is an effective method for generating pluripotent stem cells and differentiating functional therapeutic cells. This approach has great potential in disease modeling, drug screening, cell transplantation therapy, and regenerative medicine.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell & Tissue Engineering
Dipthi Bachamanda Somesh, Kristin Klose, Janita A. Maring, Desiree Kunkel, Karsten Juerchott, Stephanie I. Protze, Oliver Klein, Grit Nebrich, Matthias Becker, Ulrike Krueger, Timo Z. Nazari-Shafti, Volkmar Falk, Andreas Kurtz, Manfred Gossen, Christof Stamm
Summary: Direct cardiac reprogramming using cardiac transcription factors and molecular beacon technology successfully generated iCMP-like cells with cardioprotective potential from cardiac fibroblasts, showing potential therapeutic effects in a mouse model of acute myocardial infarction.
STEM CELL RESEARCH & THERAPY
(2023)
Article
Virology
Natalia Teresa Jarzebska, Julia Frei, Severin Lauchli, Lars E. French, Emmanuella Guenova, Cecile Gouttefangeas, Thomas M. Kundig, Mark Mellett, Steve Pascolo
Summary: This study presents a simple method for determining in vitro T-cell responses by lipofection of synthetic mRNA in mononuclear cells. The results demonstrate that antigen-specific T-cell immune monitoring can be easily and quickly performed using this method. This work provides a convenient solution for the in vitro monitoring of natural or therapy-induced T-cell immune responses.
Article
Biochemistry & Molecular Biology
Ian A. Mellis, Hailey Edelstein, Rachel Truitt, Yogesh Goyal, Lauren E. Beck, Orsolya Symmons, Margaret C. Dunagin, Ricardo A. Linares Saldana, Parisha P. Shah, Juan A. Perez-Bermejo, Arun Padmanabhan, Wenli Yang, Rajan Jain, Arjun Raj
Summary: Identifying transcription factors that maintain cell type in vitro through perturbation-induced transcriptional responses can help manipulate and control cell types effectively.
Article
Biochemistry & Molecular Biology
Lisa Sturm, Bettina Schwemberger, Ursula Menzel, Sonja Hackel, Christoph E. Albers, Christian Plank, Jaap Rip, Mauro Alini, Andreas Traweger, Sibylle Grad, Valentina Basoli
Summary: Biodegradable and bioresponsive polymer-based nanoparticles show promise for oligonucleotide delivery in mRNA-based therapeutics. The efficiency of a cationic, hyperbranched poly(amidoamine)s-based nanoparticle system in delivering tdTomato mRNA to different cell types was evaluated, with varying transfection efficiencies observed. Increasing the amount of nanoparticles generally improved transfection efficiency, but higher doses showed dose-dependent cytotoxicity in certain cell types. Transfection of extracellular matrix-rich 3D cell culture constructs using the nanoparticle system was demonstrated, suggesting a potential strategy for transfecting musculoskeletal tissues with complex extracellular matrices.
Article
Pharmacology & Pharmacy
Camille Ayad, Pierre Libeau, Celine Lacroix-Gimon, Catherine Ladaviere, Bernard Verrier
Summary: The approval of two mRNA vaccines as urgent prophylactic treatments against Covid-19 made them a realistic alternative to conventional vaccination methods. The development of an innovative vector called LipoParticles (LP) demonstrated significant advantages in cellular transfection.
Article
Cell Biology
Philipp Baumert, Matthew Cocks, Juliette A. Strauss, Sam O. Shepherd, Barry Drust, Mark J. Lake, Claire E. Stewart, Robert M. Erskine
Summary: This study identified a polygenic profile associated with muscle weakness and poorer recovery following EIMD, and provided evidence for a novel TTN gene-cell-skeletal muscle mechanism that may help explain some of the interindividual variability in the response to EIMD.
JOURNAL OF CELLULAR PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Seung-Hye Yu, Manjesh Kumar, Il Won Kim, Jeffrey D. Rimer, Tae-Jung Kim
Summary: The study evaluated the cytotoxicity of different types and compositions of zeolites on human lung fibroblast cells, showing that FAU-S exhibited higher toxicity. Different zeolites also showed significant differences in the formation of phagosomes within cells and gene expression.
Article
Medicine, Research & Experimental
Oscar Escalona-Rayo, Ye Zeng, Renzo A. Knol, Thomas J. F. Kock, Dennis Aschmann, Bram Slutter, Alexander Kros
Summary: This study examined the effects of three clinically-approved ionizable cationic lipids (ICLs) on the performance of lipid nanoparticles (LNPs) for mRNA delivery and vaccine efficacy. The results showed differences in the in vitro and in vivo performance of LNPs containing different ICLs, suggesting that in vitro data may not adequately predict their behavior in vivo.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Article
Cell & Tissue Engineering
Israa S. Salman, Ahmed Majeed Al-Shammari, Mukhtar Khamis Haba
Summary: Transdifferentiation of skin fibroblasts into insulin-producing cells (IPCs) was successfully induced using a novel chemical formula. Protein expression of pancreatic markers increased over time, with IPCs showing a promising effect in lowering blood glucose levels in diabetic mice.
CELLULAR REPROGRAMMING
(2022)
Article
Cell & Tissue Engineering
Ana Belen Alvarez-Palomo, Jordi Requena-Osete, Raul Delgado-Morales, Victoria Moreno-Manzano, Carme Grau-Bove, Agueda M. Tejera, Manel Juan Otero, Carme Barrot, Irene Santos-Barriopedro, Alejandro Vaquero, Jovita Mezquita-Pla, Sebastian Moran, Carlos Hobeich Naya, Iris Garcia-Martinez, Francisco Vidal Perez, Maria A. Blasco, Manel Esteller, Michael J. Edel
Summary: The study demonstrates that utilizing synthetic mRNA transfection of CYCLIN D1 during reprogramming repairs DNA and significantly improves the genetic stability of human iPSC. This method reduces various genetic instabilities, decreases DNA damage, promotes correct protein expression, and reduces single-nucleotide polymorphism changes per chromosome.
Article
Medicine, General & Internal
Chika Miyagi-Shiohira, Issei Saitoh, Masami Watanabe, Hirofumi Noguchi
Summary: This study found significant differences in the expression levels of key genes between iF cells and iTS-P cells in pancreatic tissue, suggesting that these differences could be important markers for distinguishing between these two cell types.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Daniel J. Owens, Julien Messeant, Sophie Moog, Mark Viggars, Arnaud Ferry, Kamel Mamchaoui, Emmanuelle Lacene, Norma Romero, Astrid Brull, Gisele Bonne, Gillian Butler-Browne, Catherine Coirault
Summary: Laminopathies are a group of disorders caused by mutations in the LMNA gene, particularly affecting skeletal and cardiac muscles. Mutations in LMNA-CMD lead to impaired myogenic fusion, defective protein synthesis, and neuromuscular junction remodeling. This study suggests that A-type lamins play a role in mechanosignaling pathways in skeletal muscle growth.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Yuichiro Tone, Kamel Mamchaoui, Maria K. Tsoumpra, Yasumasa Hashimoto, Reiko Terada, Rika Maruyama, Michael J. Gait, Andrey A. Arzumanov, Graham McClorey, Michihiro Imamura, Shin'ichi Takeda, Toshifumi Yokota, Matthew J. A. Wood, Vincent Mouly, Yoshitsugu Aoki
Summary: Researchers have established immortalized canine myoblast lines to demonstrate that a novel cell-penetrating peptide-conjugated splice-switching oligonucleotide significantly improves multiexon skipping activity. This approach could serve as a basis for future pharmacological studies on drug delivery tools.
NUCLEIC ACID THERAPEUTICS
(2021)
Article
Biochemistry & Molecular Biology
Elena Marchesi, Matteo Bovolenta, Lorenzo Preti, Massimo L. Capobianco, Kamel Mamchaoui, Monica Bertoldo, Daniela Perrone
Summary: The study found that ASO oligonucleotides conjugated with ursodeoxycholic acid (UDCA) showed better performance in targeting human DMD exon 51 compared to unconjugated ones, with an average 9.5-fold increase in exon skipping efficiency.
Article
Biochemistry & Molecular Biology
Philipp Heher, Massimo Ganassi, Adelheid Weidinger, Elise N. Engquist, Johanna Pruller, Thuy Hang Nguyen, Alexandra Tassin, Anne-Emilie Decleves, Kamel Mamchaoui, Christopher R. S. Banerji, Johannes Grillari, Andrey V. Kozlov, Peter S. Zammit
Summary: FSHD is characterized by oxidative stress induced by DUX4, leading to metabolic dysfunction and impaired mitochondrial function. Increased mitochondrial ROS levels in FSHD muscle cells are associated with elevated steady-state mitochondrial membrane potential. DUX4 triggers mitochondrial membrane polarization, resulting in mitochondrial ROS generation and apoptosis.
Article
Cell Biology
Jaione Lasa-Elgarresta, Laura Mosqueira-Martin, Klaudia Gonzalez-Imaz, Pablo Marco-Moreno, Gorka Gerenu, Kamel Mamchaoui, Vincent Mouly, Adolfo Lopez de Munain, Ainara Vallejo-Illarramendi
Summary: LGMDR1 is caused by mutations in the CAPN3 gene, leading to reduced SERCA levels. In this study, researchers found that inhibiting the ubiquitin-proteasome pathway can increase SERCA levels and potentially rescue mutant CAPN3 protein. However, treatment with a proteasome inhibitor did not effectively restore SERCA levels in an animal model.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Camille Januel, Giovanna Menduti, Kamel Mamchaoui, Cecile Martinat, Ruben Artero, Piotr Konieczny, Marina Boido
Summary: In this study, we discovered that moxifloxacin has the potential to treat spinal muscular atrophy (SMA). It improves the molecular and phenotypical defects associated with SMA and increases SMN protein levels, leading to improved motor skills and extended lifespan.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Multidisciplinary Sciences
Angelina M. Georgieva, Xinyue Guo, Marek Bartkuhn, Stefan Guenther, Carsten Kuenne, Christian Smolka, Ann Atzberger, Ulrich Gaertner, Kamel Mamchaoui, Eva Bober, Yonggang Zhou, Xuejun Yuan, Thomas Braun
Summary: The inactivation of protein deacetylase SIRT6 increases the expression of utrophin and improves dystrophic muscle pathology in mice, suggesting that epigenetic manipulation of utrophin expression could be a promising approach for the treatment of Duchenne Muscular Dystrophy (DMD).
NATURE COMMUNICATIONS
(2022)
Article
Medicine, Research & Experimental
Swati Dudhal, Lylia Mekzine, Bernard Prudhon, Karishma Soocheta, Bruno Cadot, Kamel Mamchaoui, Delphine Trochet, Marc Bitoun
Summary: Dominant centronuclear myopathy is a rare form of congenital myopathy caused by heterozygous mutations in the DNM2 gene. Specific allele-specific siRNAs have been developed to target the mutated mRNA and show beneficial effects in patient-derived cell lines.
MOLECULAR THERAPY-NUCLEIC ACIDS
(2022)
Article
Chemistry, Multidisciplinary
Muhammad Haseeb Iqbal, Faratiana Jeanne Rosine Revana, Emeline Pradel, Varvara Gribova, Kamel Mamchaoui, Catherine Coirault, Florent Meyer, Fouzia Boulmedais
Summary: The engineering of skeletal muscle tissue is a promising approach for the treatment of muscle injuries and diseases. In this study, a hydrogen-bonded nanofilm was developed using a brushing method to promote muscle tissue development. The nanofilm successfully guided the alignment and differentiation of muscle cells, resulting in the formation of aligned myofibers.
Article
Oncology
Dzhangar Dzhumashev, Andrea Timpanaro, Safa Ali, Andrea J. De Micheli, Kamel Mamchaoui, Ilaria Cascone, Jochen Rossler, Michele Bernasconi
Summary: Rhabdomyosarcoma (RMS) is a common soft tissue sarcoma in childhood and adolescence. This study explored the use of tumor-targeting peptides to selectively deliver drugs to RMS cells. Among the tested peptides, F3 showed strong and specific binding to RMS cells, effective internalization, and delivery of a toxin. The target of F3 peptide, nucleolin, was found to be expressed on the surface of RMS cells. The results suggest that F3 peptide could be a potential therapeutic approach for RMS.
Article
Multidisciplinary Sciences
Wonyul Jang, Dmytro Puchkov, Paula Samso, YongTian Liang, Michal Nadler-Holly, Stephan J. Sigrist, Ulrich Kintscher, Fan Liu, Kamel Mamchaoui, Vincent Mouly, Volker Haucke
Summary: Cells respond to fluctuating nutrient supply by changing organelle dynamics and metabolism. This study reveals that lipid signaling on the endoplasmic reticulum can control mitochondrial morphology and function, enabling cells to adapt to changing nutrient environments.
Article
Medicine, Research & Experimental
Cedric Happi Mbakam, Joel Rousseau, Yaoyao Lu, Anne Bigot, Kamel Mamchaoui, Vincent Mouly, Jacques P. Tremblay
Summary: In this study, researchers used CRISPR-Cas9 prime editing technology to correct a mutation in the DMD gene, resulting in improved editing efficiency and restoration of dystrophin protein expression. Optimization of the reverse transcription template sequence led to a significant increase in the editing percentage of the target nucleotide.
MOLECULAR THERAPY-NUCLEIC ACIDS
(2022)
Article
Medicine, Research & Experimental
Andrea Benzi, Serena Baratto, Cecilia Astigiano, Laura Sturla, Chiara Panicucci, Kamel Mamchaoui, Lizzia Raffaghello, Santina Bruzzone, Elisabetta Gazzerro, Claudio Bruno
Summary: Sarcoglycanopathies are characterized by progressive muscle degeneration. This study reveals the important role of extracellular adenosine triphosphate (eATP) and the purinoceptor P2Y2 in muscle cells from patients with a-/g-sarcoglycanopathy. Understanding the immune-mediated damage associated with these disorders is critical for developing effective gene therapies.
LABORATORY INVESTIGATION
(2023)
Article
Medicine, Research & Experimental
Francesca Tasca, Marcella Brescia, Jin Liu, Josephine M. Janssen, Kamel Mamchaoui, Manuel A. F. V. Goncalves
Summary: Genome editing based on dual CRISPR-Cas9 complexes allows for the removal of specific genomic sequences in living cells, but delivering the required reagents in a synchronous and stoichiometric manner remains challenging. The delivery of forced Cas9 heterodimers or forced CRISPR-Cas9 heterodimers using high-capacity adenovector particles (AdVPs) promotes precise multiplexing genome editing and achieves superior efficiency and accuracy compared to separate delivery of components. The integrated delivery of forced CRISPR-Cas9 heterodimers engages target sequences in a more coordinated fashion, resulting in successful gene editing outcomes.
MOLECULAR THERAPY-NUCLEIC ACIDS
(2023)
Article
Medicine, General & Internal
Cedric Happi Mbakam, Jeanne Roustant, Joel Rousseau, Pouire Yameogo, Yaoyao Lu, Anne Bigot, Kamel Mamchaoui, Vincent Mouly, Gabriel Lamothe, Jacques P. Tremblay
Summary: In this study, the CRISPR-Cas9 Prime editing technology was used to develop strategies to correct frameshift mutations in the DMD gene carrying exon 52 deletion or exons 45 to 52 deletion. Through the use of optimized epegRNAs, specific substitutions, deletions, and insertions were induced in the splice donor sites for exons 51 and 53. These modifications resulted in the skipping of exons 51 and 53 and restored the expression of dystrophin. Overall, Prime editing successfully corrected frameshift mutations in the DMD gene.
FRONTIERS IN MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Soojung Hahn, Gyuri Kim, Sang-Man Jin, Jae Hyeon Kim
Summary: This study utilized three-dimensional intestinal organoids to investigate the effects of metformin on inflammatory bowel disease (IBD) and found that metformin can enhance intestinal barrier function and reduce levels of inflammatory cytokines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
V. V. Sudarev, M. S. Gette, S. V. Bazhenov, O. M. Tilinova, E. V. Zinovev, I. V. Manukhov, A. I. Kuklin, Yu. L. Ryzhykau, A. V. Vlasov
Summary: This study investigated the self-assembly processes of ferritin-based protein complexes and obtained structurally characterized oligomeric states. These results provide new potential and opportunities for the application of ferritin in various fields.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Yalda Sabaghi, Farnaz Pourfarzad, Leila Zolghadr, Azita Bahrami, Tahereh Shojazadeh, Alireza Farasat, Nematollah Gheibi
Summary: p-Coumaric acid (p-CA) is a plant compound with anti-cancer activities. This study designed a nano-liposomal carrier containing p-CA to enhance its effectiveness against melanoma cells. The findings showed that the liposomal form of p-CA had a greater impact on the cells. Kinetic modeling indicated that the best fitting model was zero-order.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
M. D. Nazmul Hasan, Md Mahfuzur Rahman, Al Asmaul Husna, Nobuhiro Nozaki, Osamu Yamato, Naoki Miura
Summary: This study investigated the expression of ncRNAs other than miRNAs in different histologic subtypes of canine mammary gland tumors (MGT). Three aberrantly expressed ncRNAs were identified as potential biomarkers for differentiating MGT subtypes. YRNA and tRFs expression levels were found to be decreased in metastatic compared to primary MGT cell lines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Seine A. Shintani
Summary: In this study, the researchers used signal analysis to study the instantaneous amplitude and phase of sarcomeric oscillations in skeletal muscle. They identified two types of oscillations, sarcomeric oscillations and sarcosynced oscillations, and visualized their behavior during propagating waves. The researchers discovered the presence of sarcomeric defect holes and sarcomeric collision holes, which are important indicators for understanding the oscillation properties of sarcomeres. This finding has important implications for improving our understanding of muscle function and its regulatory mechanisms.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Shuanglin Zhang, Yuzhong Jia, Guolan Ma, Yanyan Yang, Zhenzhen Cao, Antao Luo, Zefu Zhang, Shihan Li, Jie Wen, Hanfeng Liu, Jihua Ma
Summary: Bupleurum is an antiarrhythmic agent that may exert its effects by inhibiting L-type calcium channels.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Tomotaka Ohkubo, Yasuhiko Matsumoto, Hiroaki Sasaki, Kaoru Kinoshita, Yuki Ogasawara, Takashi Sugita
Summary: This study found that Citrobacter koseri inhibits the growth of Staphylococcus epidermidis, disrupting the balance between S. epidermidis and Staphylococcus aureus, and exacerbating inflammation in atopic dermatitis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Toshifumi Asano, Philipp Sasse, Takao Nakata
Summary: A Cre recombination-based fluorescent reporter system was developed to monitor cell-cell fusion. The system successfully detected the formation of multinuclear myotubes and placental syncytiotrophoblast. This tool could facilitate the study of cell-to-cell fusion.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Ke Shi, Yunlong Shan, Xiao Sun, Kuida Chen, Qiong Luo, Qiang Xu
Summary: This study found that low expression of TP53INP2 is associated with poor survival in colorectal cancer (CRC) patients. As the malignancy of CRC progresses, TP53INP2 expression gradually decreases. Knockdown of TP53INP2 promotes CRC cell proliferation and tumor growth. Mechanistically, TP53INP2 deficiency decreases phosphorylation of beta-catenin, leading to increased accumulation and enhanced nuclear translocation and transcriptional activity. Additionally, TP53INP2 sequesters TIM50, inhibiting its activation of beta-catenin. In conclusion, downregulation of TP53INP2 promotes CRC progression by activating beta-catenin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Martina Rossi, Fabio Tomaselli, Alejandro Hochkoeppler
Summary: Oligomeric enzymes are known for their higher catalytic rates compared to monomeric enzymes, but the extent of additivity in their activity is still not well understood. This study used tetrameric rabbit lactate dehydrogenase as a model to examine the kinetics of its catalytic action. Surprisingly, when the concentration of the limiting reactant exceeded that of a single subunit, there was a significant slowdown in the enzyme's conformational rearrangements.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Amin Sahraei, Mohammad Javad Shamsoddini, Fakhrossadat Mohammadi, Leila Hassani
Summary: This study explored the inhibitory effects of gallium curcumin, indium curcumin, and vanadyl curcumin on the amyloid fibrillation of hen egg white lysozyme, as well as the binding interactions of these metal complexes with the enzyme. The results showed that indium curcumin and vanadyl curcumin exhibited higher binding affinities and stronger inhibitory effects on amyloid fibrillation compared to gallium curcumin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Takahiro Sasaki, Yoshiki Kuse, Shinsuke Nakamura, Masamitsu Shimazawa
Summary: PGRN deficiency plays a significant role in cardiac remodeling and arrhythmias post-myocardial infarction (MI), potentially by promoting metabolic abnormalities in macrophages.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Hongwei Zhao, Yiqiang Li, Yibo Zhang, Chi Zhang
Summary: Electrical brain stimulation technology is commonly used to treat brain neurological disorders, but it can cause side effects. This study investigated the impact of electric fields on nerve fibers and revealed the possible origin of side effects. The findings provide guidance for selecting electrical parameters in clinical stimulation therapy.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Julia S. Scott, Lake-Ee Quek, Andrew J. Hoy, Johannes V. Swinnen, Zeyad D. Nassar, Lisa M. Butler
Summary: The fatty acid elongation enzyme ELOVL5 plays a critical role in promoting metastasis in prostate cancer. Knocking down ELOVL5 leads to the accumulation of malonyl-CoA, which inhibits fatty acid oxidation in mitochondria. This study highlights the importance of fatty acid elongation in regulating cell viability and provides a potential target for prostate cancer treatment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Zan Zhou, Wen-jun Jiang, Li Li, Jun-qiang Si
Summary: This study investigates the effect of noise exposure on cognitive function in mice and explores the underlying molecular mechanisms. The findings suggest that noise exposure leads to increased inflammation, increased phosphorylation of Tau protein, and decreased levels of postsynaptic density protein, resulting in cognitive impairment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
