A Case Study to Identify the Drug Conjugation Site of a Site-Specific Antibody-Drug-Conjugate Using Middle-Down Mass Spectrometry

Top-Down Characterization of Heavily Modified Histones Using 193 nm Ultraviolet Photodissociation Mass Spectrometry

(2018) Sylvester M. Greer et al.   JOURNAL OF PROTEOME RESEARCH

Multiplexed Middle-Down Mass Spectrometry Reveals Light and Heavy Chain Connectivity in a Monoclonal Antibody

(2018) Kristina Srzentic et al.   ANALYTICAL CHEMISTRY

High-Throughput Analysis of Intact Human Proteins Using UVPD and HCD on an Orbitrap Mass Spectrometer

(2017) Timothy P. Cleland et al.   JOURNAL OF PROTEOME RESEARCH

Top-down analysis of immunoglobulin G isotypes 1 and 2 with electron transfer dissociation on a high-field Orbitrap mass spectrometer

(2017) Luca Fornelli et al.   Journal of Proteomics

Ultraviolet, Infrared, and High-Low Energy Photodissociation of Post-Translationally Modified Peptides

(2017) Mohammad A. Halim et al.   JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY

Erratum to: Analysis of Monoclonal Antibodies in Human Serum as a Model for Clinical Monoclonal Gammopathy by Use of 21 Tesla FT-ICR Top-Down and Middle-Down MS/MS

(2017) Lidong He et al.   JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY

Insights from native mass spectrometry approaches for top- and middle- level characterization of site-specific antibody-drug conjugates

(2017) Thomas Botzanowski et al.   mAbs

CAT-02-106, a Site-Specifically Conjugated Anti-CD22 Antibody Bearing an MDR1-Resistant Maytansine Payload Yields Excellent Efficacy and Safety in Preclinical Models

(2017) Penelope M. Drake et al.   MOLECULAR CANCER THERAPEUTICS

Next generation antibody drugs: pursuit of the 'high-hanging fruit'

(2017) Paul J. Carter et al.   NATURE REVIEWS DRUG DISCOVERY

Strategies and challenges for the next generation of antibody–drug conjugates

(2017) Alain Beck et al.   NATURE REVIEWS DRUG DISCOVERY

Characterization of Therapeutic Monoclonal Antibodies at the Subunit-Level using Middle-Down 193 nm Ultraviolet Photodissociation

(2016) Victoria C. Cotham et al.   ANALYTICAL CHEMISTRY

Generating aldehyde-tagged antibodies with high titers and high formylglycine yields by supplementing culture media with copper(II)

(2016) Dona York et al.   BMC BIOTECHNOLOGY

Cutting-edge mass spectrometry methods for the multi-level structural characterization of antibody-drug conjugates

(2016) Alain Beck et al.   Expert Review of Proteomics

Site-Specific Tandem Knoevenagel Condensation–Michael Addition To Generate Antibody–Drug Conjugates

(2016) Romas A. Kudirka et al.   ACS Medicinal Chemistry Letters

Generating Site-Specifically Modified Proteins via a Versatile and Stable Nucleophilic Carbon Ligation

(2015) Romas Kudirka et al.   CHEMISTRY & BIOLOGY

Reconstitution of Formylglycine-generating Enzyme with Copper(II) for Aldehyde Tag Conversion

(2015) Patrick G. Holder et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Characterization of antibody drug conjugate positional isomers at cysteine residues by peptide mapping LC–MS analysis

(2015) Marie-Claire Janin-Bussat et al.   JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES

Computational and Mass-Spectrometry-Based Workflow for the Discovery and Validation of Missing Human Proteins: Application to Chromosomes 2 and 14

(2015) Christine Carapito et al.   JOURNAL OF PROTEOME RESEARCH

Middle-Down Analysis of Monoclonal Antibodies with Electron Transfer Dissociation Orbitrap Fourier Transform Mass Spectrometry

(2014) Luca Fornelli et al.   ANALYTICAL CHEMISTRY

Top-Down Structural Analysis of an Intact Monoclonal Antibody by Electron Capture Dissociation-Fourier Transform Ion Cyclotron Resonance-Mass Spectrometry

(2013) Yuan Mao et al.   ANALYTICAL CHEMISTRY

Hydrazino-Pictet-Spengler Ligation as a Biocompatible Method for the Generation of Stable Protein Conjugates

(2013) Paresh Agarwal et al.   BIOCONJUGATE CHEMISTRY

Antibody-drug conjugate model fast characterization by LC-MS following IdeS proteolytic digestion

(2013) Elsa Wagner-Rousset et al.   mAbs

Analysis of Intact Monoclonal Antibody IgG1 by Electron Transfer Dissociation Orbitrap FTMS

(2012) Luca Fornelli et al.   MOLECULAR & CELLULAR PROTEOMICS

Site-specific chemical protein conjugation using genetically encoded aldehyde tags

(2012) David Rabuka et al.   Nature Protocols

Fast analysis of recombinant monoclonal antibodies using IdeS proteolytic digestion and electrospray mass spectrometry

(2011) Guillaume Chevreux et al.   ANALYTICAL BIOCHEMISTRY

Structural Analysis of Intact Monoclonal Antibodies by Electron Transfer Dissociation Mass Spectrometry

(2011) Yury O. Tsybin et al.   ANALYTICAL CHEMISTRY

Characterizing protein glycosylation sites through higher-energy C-trap dissociation

(2010) Zaneer M. Segu et al.   RAPID COMMUNICATIONS IN MASS SPECTROMETRY

An improved trypsin digestion method minimizes digestion-induced modifications on proteins

(2009) Da Ren et al.   ANALYTICAL BIOCHEMISTRY

Mass spectrometry for structural characterization of therapeutic antibodies

(2008) Zhongqi Zhang et al.   MASS SPECTROMETRY REVIEWS