Article
Cell Biology
Wenshu Zeng, Wenxin Zhang, Erin H. Y. Tse, Jing Liu, Anqi Dong, Kim S. W. Lam, Shaoyuan Luan, Wai Hing Kung, Tsz Ching Chan, Tom H. Cheung
Summary: Age-associated impairments in adult stem cell functions and tissue regeneration are linked to mitochondrial dysfunction and cellular senescence. The RNA-binding protein CPEB4 is downregulated in aged tissues and plays a critical role in regulating mitochondrial proteome and activity. Restoration of CPEB4 expression improves mitochondrial metabolism, enhances stem cell functions, and prevents cellular senescence, suggesting therapeutic potential for age-related senescence.
DEVELOPMENTAL CELL
(2023)
Article
Immunology
Patricia Lavandoski, Vinicius Pierdona, Rafael Moura Maurmann, Lucas Kich Grun, Fatima T. C. R. Guma, Florencia Maria Barbe-Tuana
Summary: This study provides insights into the mechanisms underlying the pro-aging effects of CCL11 in the lungs of asthmatic patients. Understanding the role of CCL11 in senescence development may have important implications for the treatment of age-related lung diseases, such as asthma.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Multidisciplinary Sciences
Sunyoung Hwang, Paola Cavaliere, Rui Li, Lihua Julie Zhu, Noah Dephoure, Eduardo M. Torres
Summary: The study reveals that mRNA and protein levels are increased by 1.5-fold on average in trisomy 21 cells, with some proteins enriched for subunits of macromolecular complexes showing signs of post-transcriptional regulation. Additionally, trisomy 21 cells exhibit lower viability and increased dependency on serine-driven lipid synthesis.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biology
Dylan C. Sarver, Cheng Xu, Susana Rodriguez, Susan Aja, Andrew E. Jaffe, Feng J. Gao, Michael Delannoy, Muthu Periasamy, Yasuhiro Kazuki, Mitsuo Oshimura, Roger H. Reeves, G. William Wong
Summary: This study investigates the impact of aneuploidy on systemic metabolism using a transchromosomic mouse model carrying a near-complete human chromosome 21. The research reveals that mice with extra copies of chromosome 21 exhibit increased calorie intake, hyperactivity, hypermetabolism, and improved insulin sensitivity. The discovery suggests that sarcolipin-mediated thermogenesis can be harnessed to promote energy expenditure and metabolic health.
Article
Biology
Minmin Song, Guangfeng Zhao, Haixiang Sun, Simin Yao, Zhenhua Zhou, Peipei Jiang, Qianwen Wu, Hui Zhu, Huiyan Wang, Chenyan Dai, Jingmei Wang, Ruotian Li, Yun Cao, Haining Lv, Dan Liu, Jianwu Dai, Yan Zhou, Yali Hu
Summary: Emerging evidence suggests a crucial role of circRNAs in regulating pathological processes, with circPTPN12 identified as a factor in endometrial fibrosis pathology. Dysfunction of the circPTPN12 pathway contributes to the pathogenesis of endometrial fibrosis.
Article
Cell Biology
Katarzyna Malgorzata Kwiatkowska, Eleni Mavrogonatou, Adamantia Papadopoulou, Claudia Sala, Luciano Calzari, Davide Gentilini, Maria Giulia Bacalini, Daniele Dall'Olio, Gastone Castellani, Francesco Ravaioli, Claudio Franceschi, Paolo Garagnani, Chiara Pirazzini, Dimitris Kletsas
Summary: The aim of this study was to characterize DNA methylation patterns in replicative and induced senescence in different human cell types. Three epigenetic signatures were identified: cell type- and treatment-specific signature, cell type-specific senescence-related signature, and cell type-transversal replicative senescence-related signature. Cluster analysis revealed distinct DNA methylation patterns in replicative senescent cells, and enrichment in pathways related to the nervous system was shown. Despite no statistically significant evidence of age acceleration, a trend of increased biological age in replicative senescent cultures of all three cell types was observed. This work highlights the heterogeneity of senescent cells and their impact on tissue homeostasis.
Article
Biochemistry & Molecular Biology
Jun-Hao Wen, Xiang-Hong He, Ze-Sen Feng, Dong-Yi Li, Ji-Xin Tang, Hua-Feng Liu
Summary: The accumulation of protein aggregates is a characteristic feature of neurodegenerative diseases. Protein homeostasis imbalance caused by proteotoxic stress or mutant proteins leads to protein aggregation, interfering with cellular processes and promoting the progression of age-related neurodegenerative diseases. Cells have evolved mechanisms to rescue or eliminate protein aggregates, and potential therapeutic strategies targeting protein aggregates are being explored.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
David W. Waters, Michael Schuliga, Prabuddha S. Pathinayake, Lan Wei, Hui-Ying Tan, Kaj E. C. Blokland, Jade Jaffar, Glen P. Westall, Janette K. Burgess, Cecilia M. Prele, Steven E. Mutsaers, Christopher L. Grainge, Darryl A. Knight
Summary: The study demonstrates that the phenomenon of senescence-induced senescence can occur in vitro in primary cultures of human lung fibroblasts, providing a possible explanation for the abnormal abundance of senescent cells in the lungs of IPF patients.
Article
Oncology
Szymon Rutecki, Martyna Pakula-Iwanska, Agnieszka Lesniewska-Bocianowska, Julia Matuszewska, Daniel Rychlewski, Pawel Uruski, Lukasz Stryczynski, Eryk Naumowicz, Sebastian Szubert, Andrzej Tykarski, Justyna Mikula-Pietrasik, Krzysztof Ksiazek
Summary: The study found that carboplatin and paclitaxel induce senescence of normal peritoneal cells and promote the proliferation, migration, and invasion of cancer cells, which is associated with the overproduction of multiple cytokines.
JOURNAL OF PATHOLOGY
(2023)
Review
Endocrinology & Metabolism
Christopher D. Wiley, Judith Campisi
Summary: Cellular senescence involves a permanent proliferative arrest and the release of biologically active molecules known as SASP. Studies show that senescence and SASP are sensitive to metabolic states, potentially driving phenotypes associated with metabolic dysfunction. Effective interventions may disrupt the feedback loop in which cellular senescence promotes metabolic diseases.
Article
Biochemistry & Molecular Biology
Fan Wu, Luyun Zhang, Caiyun Lai, Xinyue Peng, Susu Yu, Cheng Zhou, Bo Zhang, Wenjuan Zhang
Summary: N6-methyladenosine (m6A) methylation plays an important role in cellular senescence. ROS level differs between replicative and premature senescence, leading to changes in the expression profiles of m6A-related enzymes and binding proteins. Total m6A content and RNA methylation enzyme activity decrease in senescent cells. Specific m6A methylation levels regulate the expression of certain genes in replicative and premature senescence.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biotechnology & Applied Microbiology
Qunli Wang, Qi Chen, Jie Sui, Yuanyuan Tu, Xiang Guo, Feng Li
Summary: Celecoxib alleviates TNF-α-induced cellular senescence in human chondrocytes by reducing ROS production, regulating the cell cycle, restoring telomerase activity, preventing DNA damage, and suppressing the expression of p21 and p53.
Article
Nanoscience & Nanotechnology
Junlai Wan, Zhiyi He, Yingchao Zhao, Xiaoxia Hao, Jiarui Cui, Anmin Chen, Jun Zhou, Jiaming Zhang
Summary: This study investigates the potential of multi-kinase inhibitor Y099 in senescence elimination and osteoarthritis (OA) therapy. Y099 inhibits inflammation and catabolism, and promotes anabolism of chondrocytes. The delivery of Y099 by nanoliposomal hydrogel improves its performance and reduces cytotoxicity. Y099-Lip-Gel attenuates cartilage lesions and inhibits subchondral bone loss and osteoclast formation in an OA mouse model, indicating its potential for OA therapy. Transcriptomic and phosphoproteomic analyses reveal that Y099-Lip-Gel suppresses cellular senescence and inhibits MAPK and NF-kappa B signaling activations. The protective effects of Y099-Lip-Gel are also validated in human OA cartilage explants. Rating: 8/10
ADVANCED COMPOSITES AND HYBRID MATERIALS
(2023)
Article
Biochemistry & Molecular Biology
Marta Gerasymchuk, Gregory Ian Robinson, Olga Kovalchuk, Igor Kovalchuk
Summary: Modern understanding of aging is based on the accumulation of cellular damage during one's life span due to the gradual deterioration of regenerative mechanisms in response to the continuous effect of stress, lifestyle, and environmental factors, followed by increased morbidity and mortality. Cell culture models are valuable tools to investigate the mechanisms of aging by inducing cellular senescence in stress-induced premature senescence (SIPS) models.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Cell Biology
Inigo Martinez-Zubiaurre, Turid Hellevik
Summary: The tumor microenvironment (TME) plays an important role in the outcomes of radiotherapy (RT). Cancer-associated fibroblasts (CAFs), which are involved in cancer progression, may enter a senescent state after radiation. The impact of RT on CAFs and the ultimate role of irradiated CAFs in therapy outcomes are not fully understood, and there are controversies due to generalizing observations from normal fibroblasts and CAFs.
CELL COMMUNICATION AND SIGNALING
(2023)
Article
Immunology
Rigel Suzuki, Yuki Ono, Koji Noshita, Kwang Su Kim, Hayato Ito, Yuhei Morioka, Tomokazu Tamura, Daisuke Okuzaki, Tetsuzo Tagawa, Tomoyoshi Takenaka, Tomoharu Yoshizumi, Teppei Shimamura, Shingo Iwami, Takasuke Fukuhara
Summary: Smoking is closely related to the severity of COVID-19, but the connection between smoking history and SARS-CoV-2 infectivity is unknown. This study found that recent ex-smokers had higher levels of ACE2 expression in the lungs compared to non-smokers and long-term ex-smokers. The efficiency of SARS-CoV-2 infection was found to be enhanced depending on the ACE2 expression level. Gene analysis showed a correlation between smoking and the expression of inflammatory signaling genes, which decreased with longer duration of smoking cessation.
MICROBIOLOGY AND IMMUNOLOGY
(2023)
Article
Allergy
Ayaka Nakatani, Takeshi Tsuda, Yohei Maeda, Masaki Hayama, Daisuke Okuzaki, Sho Obata, Toshihiro Kishikawa, Kazuya Takeda, Hidenori Inohara
Summary: This study evaluated the role of S100A8 in the pathogenesis of eosinophilic chronic rhinosinusitis (ECRS). The results showed that S100A8 was significantly upregulated in nasal polyps associated with ECRS. Furthermore, S100A8 induced interleukin-1b secretion in human nasal epithelial cells, suggesting its involvement in the pathogenesis of ECRS.
ALLERGOLOGY INTERNATIONAL
(2023)
Article
Immunology
Takeshi Ebihara, Tsunehiro Matsubara, Yuki Togami, Hisatake Matsumoto, Jotaro Tachino, Hiroshi Matsuura, Takashi Kojima, Fuminori Sugihara, Shigeto Seno, Daisuke Okuzaki, Haruhiko Hirata, Hiroshi Ogura
Summary: This study investigated longitudinal blood proteomics and clinical information to explore the pathogenesis of COVID-19 and develop a clinical phenotype. The study identified key proteins related to disease severity and established clinical phenotypes associated with mortality and clinical outcome. These easily measured key proteins offer new insights into the pathogenesis of COVID-19 and have potential clinical applications.
JOURNAL OF CLINICAL IMMUNOLOGY
(2023)
Article
Cardiac & Cardiovascular Systems
Hiroyasu Inui, Makoto Nishida, Michiko Ichii, Hajime Nakaoka, Masumi Asaji, Seiko Ide, Shigeyoshi Saito, Ayami Saga, Takashi Omatsu, Katsunao Tanaka, Kotaro Kanno, Jiuyang Chang, Yinghong Zhu, Takeshi Okada, Daisuke Okuzaki, Takahiro Matsui, Tohru Ohama, Masahiro Koseki, Eiichi Morii, Naoki Hosen, Shizuya Yamashita, Yasushi Sakata
Summary: This study identified the crucial role of XCR1+ cDC1-activated CXCR3+ Th1 cells in ischemic cardiac remodeling.
JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY
(2023)
Article
Multidisciplinary Sciences
Jonas Norskov Sondergaard, Janyerkye Tulyeu, Ryuya Edahiro, Yuya Shirai, Yuta Yamaguchi, Teruaki Murakami, Takayoshi Morita, Yasuhiro Kato, Haruhiko Hirata, Yoshito Takeda, Daisuke Okuzaki, Shimon Sakaguchi, Atsushi Kumanogoh, Yukinori Okada, James Badger
Summary: Sex-specific differences in humoral immune responses to COVID-19 have been observed, with a higher loss of circulating follicular regulatory T cells (cTfr) in male patients. A network of cell types associated with extrafollicular antibody production is stronger in males and positively correlated with neutralizing antibody concentrations, while negatively correlated with cTfr frequency. These findings suggest that the balance of cTfr and the network of antibody-producing cell types may be a key factor in the altered humoral immune responses between male and female COVID-19 patients.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Environmental Sciences
Marie Kobayashi, Yusuke Matsuyama, Nobutoshi Nawa, Aya Isumi, Satomi Doi, Takeo Fujiwara
Summary: This study examined the association between parental social capital and access to dental check-ups among first-grade elementary school children in Japan. The findings indicate that individual-level social capital is positively associated with children's dental check-up utilization, while community-level social capital does not show a significant association.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2023)
Editorial Material
Oncology
Takahiro Tomiyama, Shinji Itoh, Norifumi Iseda, Katsuya Toshida, Yukiko Kosai-Fujimoto, Takahiro Tomino, Takeshi Kurihara, Yoshihiro Nagao, Kazutoyo Morita, Noboru Harada, Yu-Chen Liu, Daisuke Okuzaki, Kenichi Kohashi, Yoshinao Oda, Masaki Mori, Tomoharu Yoshizumi
ANNALS OF SURGICAL ONCOLOGY
(2023)
Article
Oncology
Masatoshi Kitakaze, Mamoru Uemura, Tomoaki Hara, Ryota Chijimatsu, Daisuke Motooka, Toshiro Hirai, Masamitsu Konno, Daisuke Okuzaki, Yuki Sekido, Tsuyoshi Hata, Takayuki Ogino, Hidekazu Takahashi, Norikatsu Miyoshi, Ken Ofusa, Tsunekazu Mizushima, Hidetoshi Eguchi, Yuichiro Doki, Hideshi Ishii
Summary: This study found that the number of CD103(+)/CD8(+) tumor-infiltrating lymphocytes (TILs) is a favorable prognostic factor in colorectal cancer (CRC). Additionally, they identified the expression of ZNF683 as a candidate marker for cancer-specific tissue-resident memory T (Trm) cells. The activation of Trm cells in tumors involves IFN-gamma and TCR signaling, as well as ZNF683 expression, which could be promising targets for cancer immunity regulation.
BRITISH JOURNAL OF CANCER
(2023)
Article
Biochemistry & Molecular Biology
Yusuke Tsukamoto, Yumi Nakamura, Makoto Hirata, Daisuke Okuzaki, Ryuichi Sakate, Tomonori Kimura
Summary: Each tRNA can bind to an amino acid specific to a genetic codon. It is currently unclear what factors are associated with tRNA charging and how tRNA charging is maintained. Using the individual tRNA acylation PCR method, it was discovered that the charging ratio of tRNA(Gln)(CUG) reflects the cellular glutamine level. Activation of the kinase GCN2, a key stimulator of the integrated stress response, led to upregulation of ubiquitin C (UBC) expression, which in turn suppressed the further reduction in tRNA(Gln)(CUG) charging levels. Thus, tRNA charging is sensitive to intracellular nutrient status and plays a crucial role in intracellular signaling.
Article
Immunology
Masakazu Ishikawa, Yuki Shimada, Tatsuhiko Ozono, Hisatake Matsumoto, Hiroshi Ogura, Keigo Kihara, Hideki Mochizuki, Tatsusada Okuno, Shuhei Sakakibara, Makoto Kinoshita, Daisuke Okuzaki
Summary: Recently, a case of encephalitis temporally associated with COVID-19 vaccination was reported, and single-cell RNA sequencing analysis was used to study the immune response. The analysis identified a unique myeloid cell population in peripheral blood during the acute phase of encephalitis, which was not present during remission or in healthy individuals. Further research is needed to understand the role of this myeloid subset in COVID-19 vaccination-associated autoimmunity.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Multidisciplinary Sciences
Yujiro Naito, Shohei Koyama, Kentaro Masuhiro, Takashi Hirai, Takeshi Uenami, Takako Inoue, Akio Osa, Hirotomo Machiyama, Go Watanabe, Nicolas Sax, Jordan Villa, Yumi Kinugasa-Katayama, Satoshi Nojima, Moto Yaga, Yuki Hosono, Daisuke Okuzaki, Shingo Satoh, Takeshi Tsuda, Yoshimitsu Nakanish, Yasuhiko Suga, Takayosh Morita, Kiyohar Fukushima, Masayuk Nishide, Takayuki Shiroyama, Kotaro Miyake, Kota Iwahori, Haruhiko Hirata, Izumi Nagatomo, Yukihiro Yano, Motohiro Tamiya, Toru Kumagai, Norihiko Takemoto, Hidenori Inohara, Sho Yamasaki, Kazuo Yamashita, Taiki Aoshi, Esra A. Akbay, Naoki Hosen, Yasush Shintani, Hyota Takamatsu, Masahide Mori, Yoshito Takeda, Atsushi Kumanogoh
Summary: The expression of Sema4A in tumor cells of non-small cell lung cancer is associated with a better response to PD-1 inhibitors. Sema4A enhances the cytotoxicity and proliferation of tumor-specific CD8+ T cells and improves the efficacy of PD-1 inhibitors. Therefore, Sema4A may serve as a promising therapeutic target and biomarker for predicting and promoting immune checkpoint inhibitor efficacy.
Article
Biochemistry & Molecular Biology
Haoyang Zhou, Zhiqi Xie, Naosuke Morikawa, Fuminori Sakurai, Hiroyuki Mizuguchi, Daisuke Okuzaki, Naoki Okada, Masashi Tachibana
Summary: Myeloid-derived suppressor cells (MDSCs) in tumor bearers suppress anti-tumor immunity and promote tumor progression. This study improved the in vitro method of MDSC differentiation, obtaining immunosuppressive Ly-6G+ BM-MDSC and non-immunosuppressive Ly-6G- BM-MDSC. Glutathione metabolism was found to enhance the immunosuppressive ability of Ly-6G- BM-MDSC. These findings contribute to the understanding of MDSC subpopulations and provide a basis for further research on MDSCs.
BIOCHEMISTRY AND BIOPHYSICS REPORTS
(2023)
Article
Pediatrics
Katsuya Hirata, Kimiko Ueda, Kazuko Wada, Satoyo Ikehara, Kanami Tanigawa, Tadashi Kimura, Keiichi Ozono, Tomotaka Sobue, Hiroyasu Iso
Summary: This study examined the association between gestational age classification at birth and neurodevelopmental impairments at age 3. The results showed that moderate preterm, late preterm and early term births were associated with developmental impairment in communication, motor skills, and problem-solving abilities compared to full-term births.
ARCHIVES OF DISEASE IN CHILDHOOD-FETAL AND NEONATAL EDITION
(2023)
Article
Cell Biology
Masaaki Okamoto, Miwa Sasai, Ayumi Kuratani, Daisuke Okuzaki, Masaya Arai, James B. Wing, Shimon Sakaguchi, Masahiro Yamamoto
Summary: Regulatory T (Treg) cells expressing the transcription factor (TF) Foxp3 also express other TFs shared by T helper (Th) subsets under certain conditions. VeDTR mice are generated to specifically label and deplete T-bet/Foxp3 double-positive cells. T-bet+Foxp3+ cells exhibit high resistance to oxidative stress and accumulate in tumor tissues. Short-term depletion of T-bet+Foxp3+ cells enhances anti-tumor immunity without causing autoimmunity.
Correction
Pediatrics
Katsuya Hirata, Kimiko Ueda, Kazuko Wada, Satoyo Ikehara, Kanami Tanigawa, Tadashi Kimura, Keiichi Ozono, Hiroyasu Iso
EUROPEAN JOURNAL OF PEDIATRICS
(2023)