Review
Biotechnology & Applied Microbiology
Dongdong Zhao, Xinna Zhu, Hang Zhou, Naxin Sun, Ting Wang, Changhao Bi, Xueli Zhang
Summary: Effective metabolic pathways are crucial for efficient cell factories, and utilizing CRISPR technology in pathway engineering can enhance efficiency, reduce costs, and simplify target customization. This technology has been proven to be robust and effective in various organisms.
METABOLIC ENGINEERING
(2021)
Review
Biotechnology & Applied Microbiology
Hyun Gyu Hwang, Dae-Yeol Ye, Gyoo Yeol Jung
Summary: Genetically encoded biosensors have been developed to efficiently screen enzymes with desired phenotypes, expanding enzyme discovery and engineering strategies.
BIOTECHNOLOGY ADVANCES
(2023)
Review
Biochemical Research Methods
Taehee Han, Alisher Nazarbekov, Xuan Zou, Sang Yup Lee
Summary: Systems metabolic engineering has revolutionized sustainable production through the integration of metabolic engineering with systems biology, synthetic biology, and evolutionary engineering. This article reviews the latest tools and strategies and highlights trends and challenges in the field.
CURRENT OPINION IN BIOTECHNOLOGY
(2023)
Review
Agriculture, Multidisciplinary
Wenjuan Li, Liuwei Cui, Jie Mai, Tian-Qiong Shi, Lu Lin, Zhi-Gang Zhang, Rodrigo Ledesma-Amaro, Weiliang Dong, Xiao-Jun Ji
Summary: This review summarizes the important roles and engineering strategies of Saccharomyces cerevisiae and Yarrowia lipolytica in terpene production, discusses the future prospects and challenges, and emphasizes guidelines for future studies.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Biotechnology & Applied Microbiology
Zhan-Tao Zhu, Meng-Meng Du, Bei Gao, Xin-Yi Tao, Ming Zhao, Yu-Hong Ren, Feng-Qing Wang, Dong-Zhi Wei
Summary: Harnessing mitochondria for terpene biosynthesis is promising but can lead to metabolic burden and poor cell growth. Analysis shows toxicity from MVA pathway phosphorylated intermediates within mitochondria inhibits cellular functions. Combining cytoplasmic and mitochondrial engineering strategies can alleviate metabolic burden and enhance terpene production.
METABOLIC ENGINEERING
(2021)
Article
Biotechnology & Applied Microbiology
Ye Zhang, Zihua Li, Yu Liu, Xuecong Cen, Dehua Liu, Zhen Chen
Summary: The economic viability of current bio-production systems is often limited by low productivity due to slow cell growth and low substrate uptake rate. Vibrio natriegens, a highly promising next-generation workhorse in the biotechnology industry, has been successfully engineered for heterologous production of 1,3-propanediol using systems metabolic engineering strategies. The final engineered strain can efficiently produce 1,3-PDO with high titer, yield, and productivity.
METABOLIC ENGINEERING
(2021)
Review
Biotechnology & Applied Microbiology
Chen Deng, Yaokang Wu, Xueqin Lv, Jianghua Li, Yanfeng Liu, Guocheng Du, Jian Chen, Long Liu
Summary: This review provides an overview of transcription factors, including their categories, action principles, prediction strategies, and related databases. It also highlights the applications of global transcription machinery engineering technology and transcription factor-based biosensors and quorum sensing systems. Furthermore, the review summarizes strategies for optimizing the performance of transcriptional regulatory tools by refactoring transcription factors and discusses the current limitations and prospects of constructing various regulatory tools based on transcription factors.
BIOTECHNOLOGY ADVANCES
(2022)
Review
Biotechnology & Applied Microbiology
Abhishek Joshi, Krishan K. Verma, Vishnu D. Rajput, Tatiana Minkina, Jaya Arora
Summary: Addressing climate change and energy supply issues requires the use of renewable biofuels as a replacement for petroleum fuels. However, the cost-effective conversion of lignocellulose into biofuels remains a challenge. Recent research has focused on lignocellulose feedstock and microbial engineering, with some success in improving metabolic pathways and creating robust microbial hosts. Future advancements should focus on designing more efficient pathways and screening and engineering novel enzymes to achieve economically feasible lignocellulosic-based biofuel production.
Review
Biotechnology & Applied Microbiology
Chunxiao Yan, Wei Yu, Lun Yao, Xiaoyu Guo, Yongjin J. Zhou, Jiaoqi Gao
Summary: This review summarizes the characterization and engineering of promoters in methylotrophic yeasts. It discusses the applications of constitutive and inducible promoters controlled by methanol or other inducers/repressors in protein and chemical production. It also highlights efforts in promoter engineering, such as site-directed mutagenesis, hybrid promoter, and transcription factor regulation, to expand the promoter toolbox.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Chemistry, Physical
Fei Qiu, Yijun Yan, Junlan Zeng, Jian-Ping Huang, Lingjiang Zeng, Wei Zhong, Xiaozhong Lan, Min Chen, Sheng-Xiong Huang, Zhihua Liao
Summary: The study on hyoscyamine dehydrogenase revealed the important role of the AbHDH gene in the biosynthesis of hyoscyamine and provided relevant biochemical and metabolic insights. AbHDH was found to enhance the production of hyoscyamine but did not significantly affect the biosynthesis of hyoscyamine, anisodamine, or scopolamine when suppressed.
Review
Biotechnology & Applied Microbiology
Alejandro Torres-Haro, Jorge Verdin, Manuel R. Kirchmayr, Melchor Arellano-Plaza
Summary: This paper discusses the production of astaxanthin by Xanthophyllomyces dendrorhous, emphasizing the limitations and characteristics of random and site-directed mutants in increasing astaxanthin yield. New metabolic engineering targets to enhance astaxanthin biosynthesis are proposed based on insights from metabolic, genomic, transcriptomic, and proteomic data of X. dendrorhous.
MICROBIAL CELL FACTORIES
(2021)
Review
Agricultural Engineering
Nam Kyu Kang, Kwangryul Baek, Hyun Gi Koh, Christine Anne Atkinson, Donald R. Ort, Yong-Su Jin
Summary: Microalgae are promising sustainable resources that can convert CO2 into biofuels and chemicals directly, but their industrial production and economic feasibility are still limited. Metabolic engineering approaches have been used to enhance the productivity of microalgal bioproducts, with impressive advances in the last decade. Challenges remain in achieving effective production of chemicals and fuels via microalgal metabolic engineering.
BIORESOURCE TECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Jing Kong, Lin Miao, Zhihui Lu, Shuhui Wang, Baixiang Zhao, Cuiying Zhang, Dongguang Xiao, Desmond Teo, Susanna Su Jan Leong, Adison Wong, Aiqun Yu
Summary: In this study, the oleaginous yeast Yarrowia lipolytica was metabolically engineered to produce alpha- and beta-amyrin. The production of amyrins was increased by overexpressing genes in the mevalonate pathway and enhancing the catalytic activity of the amyrin synthase enzyme. Optimization of fermentation conditions further improved the titers of alpha- and beta-amyrin. The design workflow demonstrated in this study is simple and effective for amplifying triterpenoid biosynthesis in Y. lipolytica.
MICROBIAL CELL FACTORIES
(2022)
Review
Biotechnology & Applied Microbiology
Pradipta Patra, B. R. Disha, Pritam Kundu, Manali Das, Amit Ghosh
Summary: Metabolic engineering is a powerful strategy in biotechnology, and the combination of machine learning and biological datasets allows for accurate predictions in various aspects such as strain design, gene circuits, and protein engineering. However, there are still challenges in applying machine learning in metabolic engineering.
BIOTECHNOLOGY ADVANCES
(2023)
Article
Agriculture, Multidisciplinary
Shengli Wang, Chuanling Zhan, Shengxin Nie, Daoguang Tian, Juane Lu, Mingzhang Wen, Jianjun Qiao, Hongji Zhu, Qinggele Caiyin
Summary: In this study, a highly efficient a-farnesene-producing yeast cell factory was constructed through enzyme and metabolic engineering strategies. The most active a-farnesene synthase (AFS) from Camellia sinensis (CsAFS) was introduced into Saccharomyces cerevisiae, resulting in the highest a-farnesene production. The supply of the precursor farnesyl pyrophosphate was improved by increasing the metabolic flux of the mevalonate pathway. Site-directed mutagenesis in CsAFS and the introduction of a SKIK tag further increased a-farnesene production. The final a-farnesene titer of 28.3 g/L was obtained in a 5 L bioreactor through fed-batch fermentation.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)