Journal
SMALL
Volume 15, Issue 25, Pages -Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/smll.201901397
Keywords
cell carriers; microfluidics; minimally invasive delivery; porous microspheres; tissue regeneration
Categories
Funding
- National Natural Science Foundation of China [U1605225, 31570974, 31800794]
- Natural Science Foundation of Fujian Province [2019J01076]
- Fundamental Research Funds for the Central Universities [ZQN-713]
- Program for Innovative Research Team in Science and Technology in Fujian Province University
- National Institutes of Health [R21EB026175, R01EB028143]
- Brigham Research Institute
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Microscale cell carriers have recently garnered enormous interest in repairing tissue defects by avoiding substantial open surgeries using implants for tissue regeneration. In this study, the highly open porous microspheres (HOPMs) are fabricated using a microfluidic technique for harboring proliferating skeletal myoblasts and evaluating their feasibility toward cell delivery application in situ. These biocompatible HOPMs with particle sizes of 280-370 mu m possess open pores of 10-80 mu m and interconnected paths. Such structure of the HOPMs conveniently provide a favorable microenvironment, where the cells are closely arranged in elongated shapes with the deposited extracellular matrix, facilitating cell adhesion and proliferation, as well as augmented myogenic differentiation. Furthermore, in vivo results in mice confirm improved cell retention and vascularization, as well as partial myoblast differentiation. These modular cell-laden microcarriers potentially allow for in situ tissue construction after minimally invasive delivery providing a convenient means for regeneration medicine.
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