Article
Chemistry, Analytical
Wenshuai Wu, Binh Thi Thanh Nguyen, Patricia Yang Liu, Gaozhe Cai, Shilun Feng, Yuzhi Shi, Boran Zhang, Yuzhi Hong, Ruozhen Yu, Xiaohong Zhou, Yi Zhang, Eric Peng Huat Yap, Ai Qun Liu, Lip Ket Chin
Summary: Single cell analysis is vital for disease diagnosis by searching for rare biomarkers. Microfluidic technology enables single cell analysis, but current devices have limitations such as low throughput and the need for external instruments. In this study, a self-driven high-density microwell array was developed, which significantly improved the efficiency of single-cell metabolic activity analysis.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Nanoscience & Nanotechnology
Fuyang Qu, Shirui Zhao, Guangyao Cheng, Habibur Rahman, Qinru Xiao, Renee Wan Yi Chan, Yi-Ping Ho
Summary: DEPMiC is a novel technological platform that enables convenient cultivation of multicellular spheroids and in situ characterization of the spheroids. Spheroids cultivated using DEPMiC exhibit elevated lipid ordering on the apical membrane similar to those in Matrigel counterparts, allowing for the study of drug responses in vitro-generated multicellular spheroids.
MICROSYSTEMS & NANOENGINEERING
(2021)
Article
Multidisciplinary Sciences
Nikola A. Dudukovic, Erika J. Fong, Hawi B. Gemeda, Joshua R. DeOtte, Maira R. Ceron, Bryan D. Moran, Jonathan T. Davis, Sarah E. Baker, Eric B. Duoss
Summary: Cellular fluidics offers a platform for deterministic control of multiphase flow, transport and reaction processes through unit-cell-based three-dimensional structures. By designing and manufacturing materials with structured cells, deterministic control of fluid transport in three dimensions can be achieved, potentially transforming the design space for spatial and temporal control of multiphase transport and reaction processes.
Article
Engineering, Biomedical
Pulasta Chakrabarty, Pallavi Gupta, Kavitha Illath, Srabani Kar, Moeto Nagai, Fan-Gang Tseng, Tuhin Subhra Santra
Summary: Efficient intracellular delivery strategies are crucial for biomedical applications, with recent advancements in micro/nanotechnology leading to research on microfluidic device-based strategies. Mechanoporation techniques, specifically advantageous due to their high efficiency and cell viability without external energy sources, have seen significant progress in the past two decades.
MATERIALS TODAY BIO
(2022)
Review
Chemistry, Analytical
Huimin Xie, Yuanxi Yang, Chenghao Xia, Tung-Chun Lee, Qiaosheng Pu, Yang Lan, Yuewen Zhang
Summary: Wastewater-based epidemiology is an emerging tool for monitoring public health and infectious disease outbreaks. Diffusional microfluidics is a high-throughput and cost-efficient analytical technique for protein analysis in wastewater.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2022)
Review
Biotechnology & Applied Microbiology
Haowen Yang, Jurjen Tel
Summary: This review presents recent advancements in global and local signal generators, highlighting their applications in studying temporal and spatial signaling activity, as well as the strengths and limitations of each signal generator type.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Biophysics
Run Xie, Yang Liu, Shiyu Wang, Xuyang Shi, Zhantao Zhao, Longqi Liu, Ya Liu, Zida Li
Summary: Combinatorial perturbation sequencing (CP-seq) is a high-throughput and comprehensive technology for screening combinatorial drug treatments on single cells. It utilizes the advantages of droplet microfluidics to enable efficient and multiplexed drug perturbation and transcriptome analysis.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Chemistry, Analytical
Qingxuan Li, Sidi A. Bencherif, Ming Su
Summary: This study introduces a new signal amplification mechanism based on the edge enhancement technique of microwells, which can achieve ultra-sensitive protein detection and single-cell level cytokine secretion detection, showing great potential for early disease diagnosis and treatment assessment.
ANALYTICAL CHEMISTRY
(2021)
Review
Biochemical Research Methods
Jared P. Smithers, Mark A. Hayes
Summary: The development of microfluidic manipulations has led to significant advances in the processing and detection of small volume samples. Effective isolation of single cells and manipulation of complex biomolecule mixtures allows for the utilization of information-rich detection methods. Delivering sub-nanoliter volumes of samples to a detection scheme has been achieved through spraying, meniscus-defined volumes, constrained volumes, and phase changes. This review presents selected publications highlighting the advancements and historical perspectives of these delivery techniques.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Nanoscience & Nanotechnology
Razieh Kebriaei, Amar S. Basu
Summary: This paper introduces a label-free protein detection method based on dynamic interfacial tension changes, utilizing microfluidic technology for high sensitivity and continuous monitoring. The method can be applied for direct injection of single proteins into microfluidic chips and post-column detection of protein mixtures with high performance separation techniques.
MICROFLUIDICS AND NANOFLUIDICS
(2021)
Review
Biochemical Research Methods
Jin Zhang, Jing Xue, Ningfeng Luo, Feng Chen, Badong Chen, Yongxi Zhao
Summary: Single-cell profiling is crucial for understanding cellular heterogeneity and cell fate. Microfluidics has emerged as an optimal tool for single-cell profiling due to its high throughput and automation advantages. Among different microfluidic platforms, microwell array chips offer simplicity and easy integration with in situ analysis, making them ideal for single-cell studies. This review summarizes recent advances in single-cell analysis based on microwell array chips, including chip design and preparation, cell capture and lysis strategies, as well as advanced analysis of single-cell proteins, nucleic acids, and metabolites. The challenges and opportunities in the development of microwell-based single-cell analysis are also discussed.
Article
Nanoscience & Nanotechnology
Naixin Song, Pengfei Xie, Wen Shen, Hanju Oh, Yejia Zhang, Flavia Vitale, Mehdi Javanmard, Mark G. Allen
Summary: This study introduces a microfabricated needle-shaped microwell impedance sensor for rapid and label-free detection of biomarkers, demonstrating good specificity and sensitivity for multiple proteins of interest. The mechanical characterization confirms robustness of the microneedles for transcutaneous insertion, with preserved sensor function post-insertion. Real-time in vivo quantification of human interleukin 8 (hIL8) levels in transgenic mice shows excellent agreement with ELISA serum assays.
MICROSYSTEMS & NANOENGINEERING
(2021)
Article
Computer Science, Information Systems
Pan Cao
Summary: With the increasing use of unmanned aerial vehicles, there is a growing threat of using UAVs for infrastructure/cyber-attacks and data-eavesdropping. This research explores the concept of using cellular base stations for imaging to detect UAVs, allowing them to function like inverse synthetic-aperture radars (ISAR) in addition to communication. The feasibility of this joint imaging and communication (JIAC) approach is studied and analyzed, showing promising results for UAV detection with minimal impact on current communication operations.
Article
Chemistry, Physical
Kun Yin, Meijuan Zhao, Li Lin, Yingwen Chen, Shanqing Huang, Chun Zhu, Xuan Liang, Fanghe Lin, Haopai Wei, Huimin Zeng, Zhi Zhu, Jia Song, Chaoyong Yang
Summary: Wellpaired-seq utilizes the principles of size exclusion and quasi-static hydrodynamics to improve the capture efficiency of single-cell RNA sequencing. By designing a dual well structure, it achieves high efficiency in capturing cells and beads. The technical performance of Wellpaired-seq is demonstrated by successfully revealing the heterogeneity of single cells and showing its wide applicability in basic and clinical research through transcriptome data collection from 21 samples consisting of approximately 200,000 cells.
Article
Biochemical Research Methods
Jingui Qian, Huaize Lan, Liang Huang, Shaohui Zheng, Xuefeng Hu, Minghui Chen, Joshua E. -Y. Lee, Wei Zhang
Summary: Trace biological sample detection is crucial in biomedical pathology analysis. An orthogonal tunable acoustic tweezer allows simultaneous droplet transport and centrifugation, enhancing sensitivity for biomarker detection.
Article
Biophysics
Noa Cohen, Pooja Sabhachandani, Alexander Golberg, Tania Konry
BIOSENSORS & BIOELECTRONICS
(2015)
Article
Biochemical Research Methods
S. Sarkar, P. Sabhachandani, D. Stroopinsky, K. Palmer, N. Cohen, J. Rosenblatt, D. Avigan, T. Konry
Article
Biotechnology & Applied Microbiology
Evangelia Hondroulis, Alexandru Movila, Pooja Sabhachandani, Saheli Sarkar, Noa Cohen, Toshihisa Kawai, Tania Konry
BIOTECHNOLOGY AND BIOENGINEERING
(2017)
Article
Biochemical Research Methods
P. Sabhachandani, V. Motwani, N. Cohen, S. Sarkar, V. Torchilin, T. Konry
Article
Chemistry, Analytical
Pooja Sabhachandani, Saheli Sarkar, Paola C. Zucchi, Betsy A. Whitfield, James E. Kirby, Elizabeth B. Hirsch, Tania Konry
Article
Chemistry, Analytical
Noa Cohen, Pooja Sabhachandani, Saheli Sarkar, Lindy Kahanovitz, Norman Lautsch, Steven J. Russell, Tania Konry
Article
Chemistry, Analytical
Noa Cohen, Saheli Sarkar, Evangelia Hondroulis, Pooja Sabhachandani, Tania Konry
Editorial Material
Biotechnology & Applied Microbiology
Saheli Sarkar, Pooja Sabhachandani, Tania Konry
TRENDS IN BIOTECHNOLOGY
(2017)
Article
Immunology
Saheli Sarkar, Pooja Sabhachandani, Dashnamoorthy Ravi, Sayalee Potdar, Sneha Purvey, Afshin Beheshti, Andrew M. Evens, Tania Konry
FRONTIERS IN IMMUNOLOGY
(2017)
Article
Chemistry, Multidisciplinary
Pooja Sabhachandani, Saheli Sarkar, Seamus Mckenney, Dashnamoorthy Ravi, Andrew M. Evens, Tania Konry
JOURNAL OF CONTROLLED RELEASE
(2019)
Article
Chemistry, Analytical
Saheli Sarkar, Seamus McKenney, Pooja Sabhachandani, James Adler, Xiaozhe Hu, Dina Stroopinksy, Jacalyn Rosenblatt, David Avigan, Tania Konry
SENSORS AND ACTUATORS B-CHEMICAL
(2019)
Meeting Abstract
Endocrinology & Metabolism
Lindy Kahanovitz, Noa Cohen, Pooja Sabhachandani, Norman W. Lautsch, Tania Konry, Steven J. Russell
Meeting Abstract
Hematology
Ravi Dashnamoorthy, Afshin Beheshti, Saheli Sarkar, Pooja Sabhachandani, Frank C. Passero, Sneha Purvey, Laurent Boissel, Tania Konry, Andrew M. Evens
Meeting Abstract
Chemistry, Multidisciplinary
Tania Konry, Saheli Sarkar, Pooja Sabhachandani
ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY
(2016)
