Article
Engineering, Environmental
Shunxiang Gao, Yajun Cheng, Shenghai Zhang, Xin Zheng, Jihong Wu
Summary: This study presents a biosensor-based detection method for sensitive, specific, and rapid monitoring of TNF-alpha. The biosensor exhibited a wide detection range and good reproducibility, making it a promising approach for clinical applications.
CHEMICAL ENGINEERING JOURNAL
(2022)
Article
Engineering, Multidisciplinary
Tong Hou, Hui Chang, Haoliang Jiang, Pengcheng Wang, Na Li, Yongxin Song, Dongqing Li
Summary: The smartphone integrated microfluidic lab-on-chip device enables real-time detection, counting, and sizing of living algae in ship's ballast water, achieving high accuracy and convenience in operation.
Article
Computer Science, Artificial Intelligence
Camilo A. Ruiz-Beltran, Adrian Romero-Garces, Martin Gonzalez, Antonio Sanchez Pedraza, Juan A. Rodriguez-Fernandez, Antonio Bandera
Summary: This paper presents a hardware-based embedded solution for real-time eye detection. By redesigning the popular Viola-Jones method, highly parallel single-pass image processing is achieved. Experimental validation shows that the proposed solution achieves 100% accuracy in eye detection.
EXPERT SYSTEMS WITH APPLICATIONS
(2022)
Article
Chemistry, Analytical
Mahesh P. Bhat, Mahaveer Kurkuri, Dusan Losic, Madhuprasad Kigga, Tariq Altalhi
Summary: A PDMS microfluidic channel integrated with a UV-vis fibre-optic spectrometer and a new colorimetric probe was developed for sensitive and real-time quantitative measurements of fluoride ions (F). The device features an 'S' shaped microchannel acting as a microreactor to enhance the reaction between the colorimetric probe and F ions. This optofluidic device demonstrates high sensitivity and selectivity for F ions, outperforming conventional methods in detecting real samples.
ANALYTICA CHIMICA ACTA
(2021)
Article
Multidisciplinary Sciences
Eda Capkin, Asli Kutlu, Meral Yuce
Summary: This study investigates the potential of repurposing Fc gamma RI as an analytical ligand molecule for monoclonal antibody-based biosensing. It demonstrates successful site-oriented antibody capture and real-time detection of the corresponding analyte molecule. The findings suggest that Fc gamma RI has the potential to serve as a universal ligand molecule with excellent selectivity and low nanomolar range affinity. However, surface stability and sensing response can be further improved, and further studies are required to assess its performance with different monoclonal antibodies.
Article
Multidisciplinary Sciences
R. Zamboni, A. Zaltron, M. Chauvet, C. Sada
Summary: A fully integrated opto-microfluidic platform is proposed in this study, which can detect, label, and characterize droplets without the need for imaging techniques, overcoming the limitations of traditional methods. The platform shows efficient and rapid performance in simultaneously labeling and identifying the optical properties, velocity, and lengths of each individual droplet.
SCIENTIFIC REPORTS
(2021)
Article
Chemistry, Multidisciplinary
Haimei Li, Xinyu Li, Limei Chen, Baowei Li, Hang Dong, Hongying Liu, Xueying Yang, Hiroshi Ueda, Jinhua Dong
Summary: In this study, two Quenchbody (Q-body) biosensors based on a therapeutic antibody were constructed for the rapid detection of human TNF-alpha. Both sensors were able to detect TNF-alpha within 5 minutes, showing promise for use as useful tools for the detection and monitoring of inflammatory biomarkers.
Article
Food Science & Technology
Yongjian Cheng, Bo Xie, Yifan Liang, Xinmei Liu, Haojie Chen, Jiadong Li, Hongtao Lei, Zhili Xiao
Summary: In this study, two PBZ haptens were synthesized and a highly specific monoclonal antibody against PBZ was prepared. A sensitive and rapid TRFMs-LFIA method was established for the qualitative and quantitative analysis of PBZ. The assay showed good performance in spiked wheat and rice samples and was compared to UPLC-MS/MS, demonstrating its potential as an effective strategy for on-site detection of PBZ.
CURRENT RESEARCH IN FOOD SCIENCE
(2022)
Article
Multidisciplinary Sciences
Sangam Srikanth, U. S. Jayapiriya, Satish Kumar Dubey, Arshad Javed, Sanket Goel
Summary: A simple, cost-effective and miniaturized lab-on-a-chip platform has been developed for simultaneous cultivation and detection of bacteria, with high accuracy in quantifying bacterial concentration and validating viability, as well as real-time and on-field applications.
Article
Immunology
Manivel Rengasamy, Anna Marsland, Lora McClain, Tessa Kovats, Thomas Walko, Lisa Pan, Rebecca B. Price
Summary: The study found that in adolescents receiving standard-of-care community treatment, TNF alpha levels were positively correlated with depression severity and anhedonia symptoms, with higher baseline TNF alpha levels predicting more severe depression symptoms in the future. However, no association was found between changes in clinical measures and changes in cytokine levels over time.
BRAIN BEHAVIOR AND IMMUNITY
(2021)
Article
Multidisciplinary Sciences
Helen E. Parker, Sanghamitra Sengupta, Achar Harish, Ruben R. G. Soares, Haakan N. Joensson, Walter Margulis, Aman Russom, Fredrik Laurell
Summary: Microfluidics, specifically droplet microfluidics, is a rapidly developing field that offers independent manipulation and high-throughput analysis of droplets. In this study, we propose an optofluidic Lab-in-a-Fiber scheme using a periscope fiber for stable and compact alignment. We integrate droplet microfluidics with laser-induced fluorescence detection, demonstrating the generation of monodisperse droplets and achieving a limit of detection of fluorescein. Furthermore, we show the device's capability in detecting reverse-transcription loop-mediated isothermal amplification (RT-LAMP) products for COVID-19 diagnostics, highlighting its potential as a point-of-care droplet digital RT-LAMP platform.
SCIENTIFIC REPORTS
(2022)
Article
Multidisciplinary Sciences
Ghada M. Sharif, Leon Der, Anna T. Riegel, Makarand Paranjape, Anton Wellstein
Summary: Invasion and metastatic spread of cancer cells are the main causes of cancer-related deaths. Real-Time Cellular Analysis (RTCA) technology allows for continuous measurement of cell invasion rates, providing a better understanding of invasive behavior. The tumor microenvironment, consisting of various stromal and immune cells, also plays a significant role in cancer cell invasion. By expanding dual-chamber arrays and adding an additional chamber, RTCA technology enables the measurement of invasion rates under the influence of secreted factors from co-cultured cells. This allows for the isolation and study of the most invasive cancer cells or other cell subpopulations. Molecular characteristics of these cells are crucial for mechanistic studies, diagnostic probe development, and vulnerability assessment.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2022)
Article
Computer Science, Information Systems
Yih-Shyh Chiou, Shih-Lun Chen, Wei-Ting Chen
Summary: This article proposes a low-complexity filtering algorithm and chip design for real-time location tracking. The algorithm is based on Kalman Filter (KF) and utilizes the characteristics of Kalman Gain (KG) in the alpha-beta (αβ) filtering algorithm to gradually balance the iteration. Depending on different environments, the algorithm uses Difference Kalman Filter (DKF) and Percentage Kalman Filter (PKF). It significantly reduces computing complexity and circuit area, and achieves circuit function verification on FPGA.
Article
Chemistry, Analytical
Astrid Lux, Hannah Bott, Nisar Peter Malek, Roland Zengerle, Tanja Maucher, Jochen Hoffmann
Summary: This study successfully captured and quantified circulating tumor cells in real-time through optimization of microfluidic channel design, demonstrating the significant role of real-time detection in improving quantification efficiency.
Article
Biochemistry & Molecular Biology
Mala Singh, Mohmmad Shoab Mansuri, Ashlesha Kadam, Sayantani P. Palit, Mitesh Dwivedi, Naresh C. Laddha, Rasheedunnisa Begum
Summary: TNF-alpha levels were found to be increased in vitiligo patients, impacting melanocyte biology. Stimulation with TNF-alpha resulted in reduced cell viability, increased cellular and mitochondrial ROS, and compromised complex I activity in melanocytes. TNF-alpha exposure also led to cell apoptosis and autophagy, indicating a functional link between autophagy and melanocyte destruction in autoimmune vitiligo pathogenesis.
Article
Chemistry, Applied
Mark Polikovsky, Francois Fernand, Martin Sack, Wolfgang Frey, Georg Mueller, Alexander Golberg
Article
Engineering, Biomedical
Andrey Ethan Rubin, Klimenty Levkov, Osman Berk Usta, Martin Yarmush, Alexander Golberg
ANNALS OF BIOMEDICAL ENGINEERING
(2019)
Article
Chemistry, Multidisciplinary
Pooja Sabhachandani, Saheli Sarkar, Seamus Mckenney, Dashnamoorthy Ravi, Andrew M. Evens, Tania Konry
JOURNAL OF CONTROLLED RELEASE
(2019)
Article
Chemistry, Analytical
Saheli Sarkar, Seamus McKenney, Pooja Sabhachandani, James Adler, Xiaozhe Hu, Dina Stroopinksy, Jacalyn Rosenblatt, David Avigan, Tania Konry
SENSORS AND ACTUATORS B-CHEMICAL
(2019)
Article
Biotechnology & Applied Microbiology
Alexander Chemodanov, Arthur Robin, Gabriel Jinjikhashvily, Dror Yitzhak, Alexander Liberzon, Alvaro Israel, Alexander Golberg
BIOFUELS BIOPRODUCTS & BIOREFINING-BIOFPR
(2019)
Article
Food Science & Technology
Klimentiy Levkov, Edward Vitkin, Cesar A. Gonzalez, Alexander Golberg
FOOD AND BIOPROCESS TECHNOLOGY
(2019)
Article
Multidisciplinary Sciences
Alexander Golberg, Julia Sheviryov, Oz Solomon, Leon Anavy, Zohar Yakhini
SCIENTIFIC REPORTS
(2019)
Article
Agricultural Engineering
Meghanath Prabhu, Klimentiy Levkov, Ofir Levin, Edward Vitkin, Alvaro Israel, Alexander Chemodanov, Alexander Golberg
BIORESOURCE TECHNOLOGY
(2020)
Article
Biochemical Research Methods
Rima Gnaim, Alexander Golberg, Julia Sheviryov, Boris Rubinsky, Cesar A. Gonzalez
Article
Thermodynamics
Mark Polikovsky, Amichai Gillis, Efraim Steinbruch, Arthur Robin, Michael Epstein, Abraham Kribus, Alexander Golberg
ENERGY CONVERSION AND MANAGEMENT
(2020)
Article
Thermodynamics
Alexander Golberg, Mark Polikovsky, Michael Epstein, Petronella Margaretha Slegers, Dusan Drabik, Abraham Kribus
Summary: Combining solar thermal energy systems with marine macroalgae biorefinery has shown to efficiently utilize energy and produce various renewable products. The overall efficiency of the system is 32%, which can exceed 40% with additional waste stream recycling. The prices of seaweed, electricity, and protein are key drivers of the profitability of the production process.
ENERGY CONVERSION AND MANAGEMENT
(2021)
Article
Dermatology
Sara Gouarderes, Camille Ober, Layal Doumard, Jany Dandurand, Patricia Vicendo, Isabelle Fourquaux, Alexander Golberg, Valerie Samouillan, Laure Gibot
Summary: This study demonstrates the potential of pulsed electric field technology to remodel the extracellular matrix by downregulating procollagens and activating matrix metalloproteinases. This technology shows promise for providing local and effective treatment of abnormal extracellular matrix.
JOURNAL OF INVESTIGATIVE DERMATOLOGY
(2022)
Article
Engineering, Biomedical
Batel Gabay, Klimentiy Levkov, Ariel Berl, Julia Wise, Ofir Shir-az, Edward Vitkin, Gintautas Saulis, Avshalom Shalom, Alexander Golberg
Summary: Molecular sampling with vacuum-assisted tissue electroporation is a minimally invasive method for profiling solid lesions. This study focuses on the design of a battery-powered pulsed electric field generator and electrode configuration for skin cancer diagnostics. The results demonstrate the dependence of electroporated tissue volume and protein diffusion on the electrode's geometry, penetration depths, applied electric field strength, and time after field application. These findings provide essential tools for the development of personalized skin cancer diagnostics using electroporation-based molecular markers sampling devices.
ANNALS OF BIOMEDICAL ENGINEERING
(2023)
Article
Thermodynamics
Maya Brilovich Mosseri, Amir Duenyas, Eliyahu Michael Aharon Cohen, Edward Vitkin, Efraim Steinbruch, Michael Epstein, Abraham Kribus, Michael Gozin, Alexander Golberg
Summary: This study investigates the conversion of food waste into bio-crude and biochar through hydrothermal liquefaction (HTL) to reduce its environmental impacts. The yield and properties of the products depend on the feedstock composition and process parameters. The use of catalysts and temperature can positively affect the bio-crude yield and quality. The bio-crude derived from food waste shows potential to meet a significant portion of Israel's marine fuel demand.
ENERGY CONVERSION AND MANAGEMENT-X
(2023)
Article
Food Science & Technology
Mrinal Kashyap, Supratim Ghosh, Efraim Steinbruch, Klimentiy Levkov, A'lvaro Israel, Kiran Bala, Yoav Livney, Alexander Golberg
Summary: This study presents a novel screw-press-based device for continuous high-voltage pulsed-electric-field (PEF) processing of fresh Gracilaria sp. seaweed biomass to extract water-soluble protein. The PEF-treated biomass exhibited higher protein yield in water extracts compared to extracts without PEF. PEF followed by aqueous extraction also effectively removed ash and improved protein digestibility.
ACS FOOD SCIENCE & TECHNOLOGY
(2023)
Article
Biophysics
Reza Khodadadi, Manouchehr Eghbal, Hamideh Ofoghi, Alireza Balaei, Ali Tamayol, Karen Abrinia, Amir Sanati-Nezhad, Mohamadmahdi Samandari
Summary: This paper introduces an integrated portable centrifugal microfluidic system that automates cell and fluid manipulation for complete blood counting (CBC) analysis at the point-of-care (POC). The system utilizes a specially designed microfluidic disc for cell separation, solution metering and mixing, and cell counting, and is equipped with a custom script for automated quantification of cells. The proposed method shows a strong correlation with the gold standard hematology analyzer for various blood parameters. The portable system offers simplicity, affordability, and low power consumption, making it a potential solution for improving healthcare delivery in resource-limited settings and remote areas.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Nabil H. Bhuiyan, Joon S. Shim
Summary: Developing herd immunity is crucial for changing the course of the COVID-19 pandemic. An AI-driven point-of-care testing platform has been proposed for analyzing the body's response to SARS-CoV-2, and it has been successfully used for quantifying viral proteins and antibodies in blood samples. A data-receptive web-based dashboard system has also been developed for real-time analysis of herd immunity progress.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Alexiane Godain, Timothy M. Vogel, Pascal Fongarland, Naoufel Haddour
Summary: This study provides comprehensive insights into the intricate relationship between shear stress and electroactive biofilms in microbial fuel cells, highlighting the pivotal role of shear stress in influencing the growth kinetics, electrical performance, and physical structure of the biofilms. The study also emphasizes the complexities of extracellular electron transfer mechanisms and the need for complementary metaproteomic analyses.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Linjie Wang, Yixin Chen, Yang Ji, Shujun Zheng, Fei Wang, Caolong Li
Summary: A paper-based biosensor incorporating surface-enhanced Raman spectroscopy (SERS) and colorimetric detection has been developed for efficient quantification of intracellular glutathione (GSH). The biosensor exhibits excellent selectivity, stability, and precision, with low detection limits in both SERS and colorimetric modes. It has been successfully utilized for intracellular GSH detection and validated against a commercial GSH assay kit.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Quan Zhou, Jing Wang, Zhen Zhang, Alain Wuethrich, Richard J. Lobb, Matt Trau
Summary: This study presents a biosensor for sensitive detection of EMT-associated biomarkers on extracellular vesicles (EVs) surfaces during targeted therapy. Through longitudinal monitoring of patients, the biosensor shows its ability to identify the EMT-like phenotype switching on circulating EVs during the development of resistance.
BIOSENSORS & BIOELECTRONICS
(2024)
Review
Biophysics
Pratika Rai, Sabrina N. Hoba, Celine Buchmann, Robert J. Subirana-Slotos, Christian Kersten, Tanja Schirmeister, Kristina Endres, Bernd Bufe, Alexey Tarasov
Summary: Proteases have been proposed as potential biomarkers for various pathological conditions. The development of protease biosensors offers a more efficient way to investigate dysregulated proteases. This review article presents different optical and electrochemical detection methods for designing biosensors for all major protease families.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Chengxin Liu, Xu Shen, Li Yan, Runlian Qu, Yue Wang, Yaqin He, Zixuan Zhan, Piaopiao Chen, Feng Lin
Summary: In this study, a homogeneous rapid electrochemical aptasensor was developed to quantitatively detect CTCs in lung cancer patients. The aptasensor utilized a DNA nanosphere structure and a complementary aptamer to specifically detect mucin 1 as a marker for CTCs. The assay exhibited high specificity and sensitivity, and the results were consistent with other detection methods.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Danni Xue, Xing Dai, Jialong Zhao, Jiayao Zhang, Huan Liu, Kui Liu, Tao Xu, Chenjie Gu, Xingfei Zhou, Tao Jiang
Summary: In this study, a dual-enhancement SERS substrate based on Ti3C2TX and Ag nanocubes was fabricated for precise quantification of ritonavir and ibrutinib in serum. The formation of numerous electromagnetic hotspots between Ag nanocubes facilitated effective photo-induced charge transfer. The composite substrate showed excellent sensitivity, achieving low detection limits and high recoveries, making it promising for monitoring and identification of clinical blood drug concentration.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Tenghua Zhang, Zihui Xie, Xiaohe Zheng, Yuxin Liang, Yao Lu, Hankang Zhong, Feiyang Qian, Yuqing Zhu, Ruiting Sun, Yan Sheng, Jiaming Hu
Summary: This study reports a technology based on cationic lipid-polymer hybrid nanoparticles for efficient extracellular vesicle (EV) enrichment and in-situ detection of internal microRNAs. The technology demonstrates high EV enrichment efficiency and sensitive internal RNA detection, making it potentially useful for early pancreatic cancer diagnosis.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Wenjie Dai, Gaoxu Chen, Xiaoyan Wang, Shujun Zhen, Chengzhi Huang, Lei Zhan, Yuanfang Li
Summary: In this study, a novel metal organic gel (MOG) with dual electrochemiluminescence (ECL) properties was prepared by simple mixing. The MOG exhibited strong and stable anodic and cathodic ECL signals. By utilizing this MOG, an ECL resonance energy transfer (ECL-RET) biosensor was constructed for ultrasensitive detection of a specific gene. The study developed a straightforward technique for obtaining a single luminescent material with dual signals and expanded the analytical application of MOGs in the realm of ECL.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Lais Canniatti Brazaca, Amanda Hikari Imamura, Rodrigo Vieira Blasques, Jessica Rocha Camargo, Bruno Campos Janegitz, Emanuel Carrilho
Summary: The use of microfluidic paper-based analytical devices (muPADs) for medical diagnosis is a growing trend due to their low cost, easy use, simple manufacturing, and potential for application in low-resource settings. This review focuses on the advances in muPADs for medical diagnostics, discussing their use in detecting various biomarkers in common human biofluids. The challenges of biomarker detection in each sample are examined, along with innovative techniques to overcome these limitations. The commercialization difficulties of muPADs are also considered, along with future trends such as wearable devices and integrated platforms.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Minghong Jian, Xudong Sun, Hua Zhang, Xiaotong Li, Shasha Li, Zhenxin Wang
Summary: Matrix metalloproteinases (MMPs) are attractive biomarkers for cancer diagnosis and treatment, but their low abundance in biological samples, especially in the early stages of tumors, makes it challenging to precisely analyze MMP activities. In this study, a peptide microarray-based metal-enhanced fluorescence assay (PMMEFA) is proposed as a sensitive and specific method to simultaneously detect MMP-1, -2, -3, -7, -9, and -13 activities. The PMMEFA showed excellent sensitivity and was capable of detecting MMP activities in various matrices.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Kyung Ho Kim, Eunsu Ryu, Zinah Hilal Khaleel, Sung Eun Seo, Lina Kim, Yong Ho Kim, Hyun Gyu Park, Oh Seok Kwon
Summary: We have developed a novel strategy for discriminative detection of SARS-CoV-2 variants using the plasmonic photothermal effect of gold nanofilms and digital polymerase chain reaction (dPCR) technology. With this method, we were able to detect the delta and omicron variants with high sensitivity within 25 minutes from COVID-19 patients' clinical samples, making it a rapid and accurate point-of-care testing tool.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Zepeng Kang, Yuanming Wang, Haiyan Song, Xueli Wang, Job Zhang, Zhiguang Zhu
Summary: By designing a wearable and flexible lactic-acid/O2 EBFC with an air-breathing biocathode, the limitations of biocathode are effectively solved. The optimal performance conditions are determined through experiments, and the EBFC is successfully applied to power a low-power device.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Huayun Chen, Zhiheng You, Yuhui Hong, Xiao Wang, Mingming Zhao, Yushi Luan, Yibin Ying, Yixian Wang
Summary: This study developed a colorimetric sensor array using gas-responsive two-dimensional metal-organic framework (MOF) composites for highly sensitive detection of volatile organic compounds (VOCs). The dye/Zn-2(bim)(4) composites-based sensor arrays showed enhanced sensitivity and anti-interference properties. The findings demonstrate the potential use of dye/Zn-2(bim)(4) sensor arrays for early perception of plant diseases.
BIOSENSORS & BIOELECTRONICS
(2024)