Article
Multidisciplinary Sciences
Eudald Pascual-Carreras, Marta Marin-Barba, Sergio Castillo-Lara, Pablo Coronel-Cordoba, Marta Silvia Magri, Grant N. Wheeler, Jose Luis Gomez-Skarmeta, Josep F. Abril, Emili Salo, Teresa Adell
Summary: Any planarian fragment can regenerate the missing head and tail properly by activating the Wnt/beta-catenin signaling pathway, which changes the chromatin accessibility of the wound cells. The determination of missing tissue identity relies on pre-existing tissue. Planarians are perfect organisms for studying this mechanism, as the same group of cells can regenerate a head or a tail depending on the injured body part. The differential activation of the Wnt/beta-catenin signal after amputation specifies anterior or posterior identity. The chromatin accessibility of wound cells shifts according to the polarity of pre-existing tissue in a Wnt/beta-catenin-dependent manner within 12 hours.
NATURE COMMUNICATIONS
(2023)
Article
Immunology
Karen Gu, Carina Walpole, Shayarana Gooneratne, Xin Liu, Oscar L. Haigh, Kristen J. Radford, Mark Mw Chong
Summary: DROSHA plays a miRNA-independent critical function in human HSCs by directly recognizing and degrading mRNAs, with the degradation of MYL9 mRNA alone not being essential for human HSC function.
CLINICAL & TRANSLATIONAL IMMUNOLOGY
(2022)
Article
Cell Biology
Jun Shen, Yaoyao Zhu, Shuo Zhang, Shuzhen Lyu, Cuicui Lyu, Zicen Feng, Dixie L. Hoyle, Zack Z. Wang, Tao Cheng
Summary: Objectives Vitronectin (VTN) has been shown to promote efficient haematopoietic differentiation of human pluripotent stem cells (hPSCs) by promoting the fate determination of endothelial-haematopoietic lineage during mesoderm development. Signals through alpha v beta 3 and alpha v beta 5 integrins are required for VTN-promoted haematopoietic differentiation. Knockdown experiments confirmed that alpha v beta 3 and alpha v beta 5 act synergistically for early haematopoietic differentiation.
CELL PROLIFERATION
(2021)
Article
Cell Biology
Jinping Huang, Xiao Sheng, Zhangpeng Zhuo, Danqing Xiao, Kun Wu, Gang Wan, Haiyang Chen
Summary: This study identifies ClC-c as a regulator of intestinal stem cell proliferation in Drosophila, providing new insights into the functional relationship between chloride channels, stem cell maintenance, and tissue homeostasis.
CELL PROLIFERATION
(2022)
Article
Cell Biology
Carl A. Mitchell, Evgenia V. Verovskaya, Fernando J. Calero-Nieto, Oakley C. Olson, James W. Swann, Xiaonan Wang, Aurelie Herault, Paul V. Dellorusso, Si Yi Zhang, Arthur Flohr Svendsen, Eric M. Pietras, Sietske T. Bakker, Theodore T. Ho, Berthold Gottgens, Emmanuelle Passegue
Summary: Haematopoietic ageing is characterized by a loss of regenerative capacity and abnormal differentiation of haematopoietic stem cells (HSCs), resulting in impaired blood production. The study reveals an inflammatory environment in the old bone marrow niche, which contributes to niche deterioration, HSC dysfunction, and impaired haematopoietic regeneration. The production of interleukin-1 beta (IL-1 beta) by damaged endosteum drives the proinflammatory nature of the central marrow, causing damage to the old blood system. Blocking IL-1 signaling is a potential strategy to improve blood production during ageing.
NATURE CELL BIOLOGY
(2023)
Article
Cell Biology
Xue Nan, Bowen Zhang, Jie Hao, Wen Yue, Boqiang Fu, Mingyi Qu, Yu Zhang, Haiyang Wang, Fang Fang, Jun Wei, Qiyuan Li, Shijun Hu, Junying Yu, Yingdai Gao, Qifa Liu, Jiani Cao, Lei Wang, Yaojin Peng, Huanxin Zhu, Lingmin Liang, Aijin Ma, Jiaxi Zhou, Tongbiao Zhao, Xuetao Pei
Summary: The guidelines set technical requirements and quality control standards for human haematopoietic stem/progenitor cells, aiming to promote protocol execution and international standardization.
CELL PROLIFERATION
(2022)
Article
Cell Biology
Yu Wei Zhang, Julian Mess, Nadim Aizarani, Pankaj Mishra, Carys Johnson, Mari Carmen Romero-Mulero, Jasmin Rettkowski, Katharina Schoenberger, Nadine Obier, Karin Jaecklein, Nadine M. Woessner, Maria-Eleni Lalioti, Talia Velasco-Hernandez, Katarzyna Sikora, Ralph Waesch, Bernhard Lehnertz, Guy Sauvageau, Thomas Manke, Pablo Menendez, Sebastian Gottfried Walter, Susana Minguet, Elisa Laurenti, Stefan Guenther, Dominic Gruen, Nina Cabezas-Wallscheid
Summary: This study uses single-cell RNA sequencing to investigate the dormancy state of HSCs in human bone marrow and identifies the GPRC5C receptor and its signaling pathway with hyaluronic acid as important factors in maintaining dormancy.
NATURE CELL BIOLOGY
(2022)
Article
Biology
Anna Osnato, Stephanie Brown, Christel Krueger, Simon Andrews, Amanda J. Collier, Shota Nakanoh, Mariana Quiroga Londono, Brandon T. Wesley, Daniele Muraro, A. Sophie Brumm, Kathy K. Niakan, Ludovic Vallier, Daniel Ortmann, Peter J. Rugg-Gunn
Summary: TGF beta signaling is essential for maintaining naive human pluripotent stem cells, with downstream effector proteins SMAD2/3 binding to common sites in both naive and primed cells. Inhibition of TGF beta signaling leads to downregulation of target genes and differentiation of pluripotent cells, with different transcriptional trajectories observed between naive and primed cells. These findings indicate a continuum of TGF beta pathway function in human pluripotency.
Article
Multidisciplinary Sciences
Kathleen Shah, Muralidhara Rao Maradana, M. Joaquina Delas, Amina Metidji, Frederike Graelmann, Miriam Llorian, Probir Chakravarty, Ying Li, Mauro Tolaini, Michael Shapiro, Gavin Kelly, Chris Cheshire, Deendyal Bhurta, Sandip B. Bharate, Brigitta Stockinger
Summary: The aryl hydrocarbon receptor (AHR) plays a crucial role in regulating physiological processes in the intestine, influencing tissue regeneration and the acquisition of mature epithelial cell identity after injury.
NATURE COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Veronica Moncho-Amor, Probir Chakravarty, Christophe Galichet, Ander Matheu, Robin Lovell-Badge, Karine Rizzoti
Summary: The study reveals the role of SOX2 in endocrine tumorigenesis in p27(-/-) pituitaries, showing that increased levels of SOX2 in both endocrine and stem cells are associated with pituitary hyperplasia. SOX2 is required cell autonomously in endocrine cells for tumor formation and in stem cells for promotion of tumorigenesis. Single cell transcriptomic analysis further highlights the importance of the SOX2-dependent MAPK pathway in stem cells for tumorigenesis.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Editorial Material
Cell Biology
Yang Yang, Eileen White
Summary: Macroautophagy/autophagy defects are a risk factor for inflammatory bowel disease. Studies have shown that the deletion of autophagy genes can lead to the loss of intestinal stem cells and barrier function, contributing to the development of IBD.
Article
Oncology
Takaaki Yoshikawa, Akihisa Fukuda, Mayuki Omatsu, Mio Namikawa, Makoto Sono, Yuichi Fukunaga, Tomonori Masuda, Osamu Araki, Munemasa Nagao, Satoshi Ogawa, Kenji Masuo, Norihiro Goto, Yukiko Hiramatsu, Yu Muta, Motoyuki Tsuda, Takahisa Maruno, Yuki Nakanishi, Kenji Kawada, Shigeo Takaishi, Hiroshi Seno
Summary: Brg1 is crucial for intestinal TSCs in mice by inhibiting apoptosis and is critical for cell survival and stem cell features in human CRC cells, representing a new therapeutic target for human CRC.
JOURNAL OF PATHOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Meng Zhu, Qiwei Wang, Tianning Gu, Yingli Han, Xin Zeng, Jinxin Li, Jian Dong, He Huang, Pengxu Qian
Summary: Haematopoietic Stem cells (HSCs) possess the ability to renew and differentiate into various lineages, which is regulated by the microenvironment. The use of hydrogels in tissue engineering has allowed for the mimicking of the HSC microenvironment in vitro, influencing HSC behavior and ultimately improving HSC acquisition. This review presents the recent advancements in hydrogel-based microenvironment engineering of HSCs and discusses future challenges in basic research and clinical practice.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2023)
Article
Microbiology
Emma Poole, Maria Cristina Carlan da Silva, Chris Huang, Marianne Perera, Sarah Jackson, Ian J. Groves, Mark Wills, Amer Rana, John Sinclair
Summary: This research highlights the importance of BMPR2 and YY1 in maintaining HCMV latency, showing that BMPR2 signaling through the SMAD4/SMAD6 axis and miRNA regulation play crucial roles. Pharmacological targeting of BMPR2 may represent a new therapeutic strategy for controlling HCMV latency in myeloid progenitors.
Article
Oncology
Avik Dutta, Yue Yang, Bao T. Le, Yifan Zhang, Omar Abdel-Wahab, Chongzhi Zang, Golam Mohi
Summary: The study reveals the crucial role of U2AF1 in normal hematopoiesis, as its deletion leads to loss of hematopoietic stem/progenitor cells and increased DNA damage. RNA sequencing analysis shows significant alterations in genes related to HSC maintenance, cell proliferation, and DNA damage response in U2AF1-deficient HSPC, as well as splicing alterations in genes important for HSPC function.
Article
Chemistry, Applied
Julia M. Gauglitz, Christine M. Aceves, Alexander A. Aksenov, Gajender Aleti, Jehad Almaliti, Amina Bouslimani, Elizabeth A. Brown, Anaamika Campeau, Andres Mauricio Caraballo-Rodriguez, Rama Chaar, Ricardo R. da Silva, Alyssa M. Demko, Francesca Di Ottavio, Emmanuel Elijah, Madeleine Ernst, L. Paige Ferguson, Xavier Holmes, Alan K. Jarmusch, Lingjing Jiang, Kyo Bin Kang, Irina Koester, Brian Kwan, Jie Li, Yueying Li, Alexey Melnik, Carlos Molina-Santiago, Bohan Ni, Aaron L. Oom, Morgan W. Panitchpakdi, Daniel Petras, Robert Quinn, Nicole Sikora, Katharina Spengler, Bahar Teke, Anupriya Tripathi, Sabah Ul-Hasan, Justin J. J. van Der Hooft, Fernando Vargas, Alison Vrbanac, Anthony Q. Vu, Steven C. Wang, Kelly Weldon, Kayla Wilson, Jacob M. Wozniak, Michael Yoon, Nuno Bandeira, Pieter C. Dorrestein
Article
Biochemical Research Methods
Deepank R. Korandla, Jacob M. Wozniak, Anaamika Campeau, David J. Gonzalez, Erik S. Wright
Article
Biochemistry & Molecular Biology
Robert H. Mills, Jacob M. Wozniak, Alison Vrbanac, Anaamika Campeau, Benoit Chassaing, Andrew Gewirtz, Rob Knight, David J. Gonzalez
Article
Multidisciplinary Sciences
Ying Lin, Jacob M. Wozniak, Neil J. Grimsey, Sravan Girada, Anand Patwardhan, Olivia Molinar-Inglis, Thomas H. Smith, John D. Lapek, David J. Gonzalez, Joann Trejo
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2020)
Article
Infectious Diseases
Jacob M. Wozniak, Tatiana Araujo Silva, Diane Thomas, Jair L. Siqueira-Neto, James H. McKerrow, David J. Gonzalez, Claudia M. Calvet
PLOS NEGLECTED TROPICAL DISEASES
(2020)
Article
Multidisciplinary Sciences
Jenna Hicks, Jessica Dewey, Yaniv Brandvain, Anita Schuchardt
Article
Medicine, General & Internal
Joshua D. Crapser, Elizabeth E. Spangenberg, Rocio A. Barahona, Miguel A. Arreola, Lindsay A. Hohsfield, Kim N. Green
Article
Biochemistry & Molecular Biology
Jacob M. Wozniak, Robert H. Mills, Joshua Olson, J. R. Caldera, Gregory D. Sepich-Poore, Marvic Carrillo-Terrazas, Chih-Ming Tsai, Fernando Vargas, Rob Knight, Pieter C. Dorrestein, George Y. Liu, Victor Nizet, George Sakoulas, Warren Rose, David J. Gonzalez
Article
Cell Biology
Christian de Groot, Jack Houston, Bethany Davis, Adina Gerson-Gurwitz, Joost Monen, Pablo Lara-Gonzalez, Karen Oegema, Andrew K. Shiau, Arshad Desai
Summary: The extended N-terminal tail of CENP-A in Caenorhabditis elegans plays a crucial role in kinetochore assembly and chromosome condensation by interacting directly with KNL-2 protein. Results demonstrate that the N-terminal tail of CENP-A contains a structured region predicted to be essential for centromeric chromatin assembly, highlighting an evolutionary variation in centromere structure and function in the absence of a specific CENP-A chaperone/targeting factor.
MOLECULAR BIOLOGY OF THE CELL
(2021)
Article
Multidisciplinary Sciences
Brandon E. Aubol, Jacob M. Wozniak, Laurent Fattet, David J. Gonzalez, Joseph A. Adams
Summary: The study reveals that CLK1 phosphorylates Ser-226 in U1-70K to facilitate early spliceosome assembly. This phosphorylation event releases U1-70K from subnuclear granules, allowing it to interact with other proteins and carry out key biological functions.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemistry & Molecular Biology
Cara C. Rada, Hilda Mejia-Pena, Neil J. Grimsey, Isabel Canto Cordova, Joshua Olson, Jacob M. Wozniak, David J. Gonzalez, Victor Nizet, JoAnn Trejo
Summary: Vascular inflammation can disrupt endothelial barrier integrity and cause tissue edema through the activation of PAR1 and p38 MAPK signaling pathways. The phosphorylation and function of HSP27, a key protein regulating endothelial barrier integrity, are differentially controlled by effector kinases MK2 and MK3.
Article
Education, Scientific Disciplines
Jenna Hicks, Jessica Dewey, Michael Abebe, Yaniv Brandvain, Anita Schuchardt
Summary: The study found that many students struggle to correctly apply a quantitative understanding of variation to statistically analyze biological data, and suggest that instruction focusing on conceptual understanding may improve students' quantitative understanding of variation.
JOURNAL OF MICROBIOLOGY & BIOLOGY EDUCATION
(2021)
Article
Multidisciplinary Sciences
Jenna Hicks, Jessica Dewey, Michael Abebe, Maxwell Kramer, Anita Schuchardt
Summary: The study finds that intervention through curriculum and PD can improve teaching practices of teaching assistants (TAs), even when they are teaching curriculum that is not linked to PD. TAs who participated in the intervention implemented more student-centered teaching practices. The results suggest that investment in curriculum-linked TA PD and development of higher-cognitive-demand tasks can effectively support implementation of student-centered practices.
Article
Multidisciplinary Sciences
Rocio A. Barahona, Samuel Morabito, Vivek Swarup, Kim N. Green
Summary: Microglia play a role in the regulation of perineuronal nets (PNNs) but not in the maintenance of diurnal rhythms.
SCIENTIFIC REPORTS
(2022)
Article
Education, Scientific Disciplines
Jessica Dewey, Jenna Hicks, Anita Schuchardt
Summary: This study examines the effect of a curricular intervention on students' conceptual and quantitative understanding of variation in the context of experimental design and analysis. The results show that students who received the intervention had significantly higher normalized gains in their understanding of variation compared to those who did not receive the intervention.
CBE-LIFE SCIENCES EDUCATION
(2022)