Article
Biochemistry & Molecular Biology
Alina A. Sofronova, Denis V. Pozdyshev, Kseniya V. Barinova, Vladimir I. Muronetz, Pavel I. Semenyuk
Summary: The study found that glycation of GAPDH inhibits its interaction with alpha-synuclein and RNA, potentially influencing the interplay between diabetes and neurodegenerative diseases.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2021)
Article
Chemistry, Multidisciplinary
Astrid M. Perez, Jacob A. Wolfe, Janse T. Schermerhorn, Yiwen Qian, Bekim A. Cela, Cody R. Kalinowski, Garrett E. Largoza, Peter A. Fields, Gabriel S. Brandt
Summary: This study found that the thermal stability of a critical metabolic enzyme in the stony coral Acropora millepora significantly increases in the presence of its cofactor NAD(+). The determination of the complex structure revealed variable NAD(+) occupancy across the tetrameric enzyme, with regions of difference identified that may account for the increased thermal stability.
Article
Microbiology
Shotaro Isozaki, Hiroaki Konishi, Hiroki Tanaka, Chikage Yamamura, Kentaro Moriichi, Naoki Ogawa, Mikihiro Fujiya
Summary: This study demonstrated that heptelidic acid (HA) derived from the probiotic Aspergillus oryzae exerts antitumor effects against extraintestinal melanoma cells both in vitro and in vivo. Oral administration of HA significantly suppressed the growth of transplanted melanoma tumors, indicating a potential novel system for regulating extraintestinal tumors through gut bacteria-derived bioactive mediators.
Article
Immunology
David Salcines-Cuevas, Hector Teran-Navarro, Ricardo Calderon-Gonzalez, Paula Torres-Rodriguez, Raquel Tobes, Manuel Fresno, Jorge Calvo-Montes, I. Concepcion Perez Del Molino-Bernal, Sonsoles Yanez-Diaz, Carmen Alvarez-Dominguez
Summary: The N-terminal 22 amino acid peptides of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) are highly homologous among pathogenic bacteria like Listeria, Mycobacterium, and Streptococcus, making them ideal candidates for universal vaccine designs. Vaccines containing these peptides demonstrated high immunogenicity, lack of toxicity, and cross-protection immunity in mouse models infected with different pathogens.
Article
Biochemistry & Molecular Biology
Ines Baptista, Effrosyni Karakitsou, Jean-Baptiste Cazier, Ulrich L. Guenther, Silvia Marin, Marta Cascante
Summary: Through TKTL1 knockdown experiments, it was found that TKTL1 plays a key role in the metabolic adaptation to hypoxia in THP-1 AML cells by modulating the activities of G6PD and GAPDH, which involve changes in redox capacity and transcriptional regulation of key enzymes and transporters related to glucose, PPP, and amino acid metabolism.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biotechnology & Applied Microbiology
Sang Hyun Kim, Jeong Hyeon Hwang, Hyun Joong Kim, Suk Jin Oh, Hyun Jin Kim, Nara Shin, Sang-Hyoun Kim, Jeong-Hoon Park, Shashi Kant Bhatia, Yung-Hun Yang
Summary: The study developed Clostridium acetobutylicum as a superior biohydrogen producer by introducing glyceraldehyde-3-phosphate dehydrogenase (GAPDH) to enhance hydrogen production. The strain CAC824-G containing gapC showed a 66.3% higher hydrogen production than the wild-type strain, with increased NADH and NADPH pools. Overexpression of gapC resulted in increased hydrogen production even in the presence of inhibitors.
ENZYME AND MICROBIAL TECHNOLOGY
(2023)
Article
Multidisciplinary Sciences
Christine C. Winterbourn, Alexander Peskin, Torsten Kleffmann, Rafael Radi, Paul E. Pace
Summary: The presence of bicarbonate enhances the inactivation of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) by hydrogen peroxide (H2O2) in a reversible manner. The enhanced inactivation is most likely due to the formation of a more reactive oxidant, peroxy-monocarbonate (HCO4-). GAPDH may facilitate the formation and/or targeting of HCO4- to promote its own inactivation.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Cell Biology
Xia Ding, Lihua Wang, Mingjiao Chen, Yue Wu, Shengfang Ge, Jin Li, Xianqun Fan, Ming Lin
Summary: Melanoma cells exhibit increased aerobic glycolysis, where GAPDHS plays a crucial role in regulating glycolysis. Knockdown of GAPDHS hindered glycolysis and cell proliferation, while overexpression promoted glycolysis and cell growth. SOX10 regulates the activation of GAPDHS and influences the malignant phenotype of uveal melanoma.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Yue Dai, Angela S. Fleischhacker, Liu Liu, Sara Fayad, Amanda L. Gunawan, Dennis J. Stuehr, Stephen W. Ragsdale
Summary: GAPDH is involved in heme delivery to HO2, maintaining cellular heme homeostasis.
BIOLOGICAL CHEMISTRY
(2022)
Article
Cell Biology
Ivona Pavkova, Monika Kopeckova, Marek Link, Erik Vlcak, Vlada Filimonenko, Lenka Lecova, Jitka Zakova, Pavlina Laskova, Valeria Sheshko, Miloslav Machacek, Jiri Stulik
Summary: This study discovered the involvement of Francisella tularensis GAPDH homologue GapA in multiple pathogenic processes at the host cell level. The localization of GapA was demonstrated using immunoelectron microscopy, and its interaction with multiple host cellular proteins was identified using quantitative proteomic approaches. The impact of GapA deletion on cytokine gene transcription and signaling pathway activation was investigated. The results showed that GapA enhanced the transcription of various cytokines, which was not observed in cells infected with a fully virulent wild-type strain. This study provides evidence that GapA is directly involved in multiple host cellular processes and contributes to various molecular mechanisms of pathogenesis.
Article
Parasitology
Wenhao Huang, Shunhai Zhu, Ting Chen, Qiping Zhao, Hui Dong, Bing Huang, Yawen Yao, Zhan Liu, Yu Yu, Hongyu Han
Summary: In this study, the researchers cloned and characterized the EtGAPDH gene of E. tenella and investigated its expression levels in different developmental stages and drug-resistant strains. The results showed that EtGAPDH was upregulated in drug-resistant strains, suggesting its potential involvement in the development of resistance to anticoccidial drugs.
PARASITOLOGY RESEARCH
(2022)
Article
Parasitology
Hao Jie, Si -ming Zhang, Fan-rong Ding, Chun-lian Tang, Xiang -you Li
Summary: This study evaluated the effect of SjGAPDH on the growth of schistosomula and found that SjGAPDH may affect the growth of schistosomula and could be a useful target for treating schistosomiasis.
Article
Chemistry, Physical
Adnan Ayna, Peter C. E. Moody
Summary: The study elucidates the X-ray crystal structure of Campylobacter jejuni phosphorylating glyceraldehyde 3-phosphate dehydrogenase, and investigates its role in the pathogen's metabolism.
JOURNAL OF MOLECULAR STRUCTURE
(2021)
Article
Plant Sciences
Cong Liu, Yanfeng Wang, Yuanyuan Du, Zhensheng Kang, Jia Guo, Jun Guo
Summary: In this study, a Pst effector protein was found to manipulate plant defense by interacting with key plant proteins. This effector protein regulated two different ROS signaling pathways by interacting with two wheat proteins, affecting the plant's resistance to Pst. Additionally, the effector protein stabilized a protein that suppresses ROS accumulation by inhibiting protein degradation. These findings revealed the mechanism behind how Pst promotes its infection by manipulating plant proteins.
PLANT CELL AND ENVIRONMENT
(2023)
Article
Biophysics
Lu-Liang Wang, Minting Fan, Xin Xing, Yushen Liu, Shuyang Sun
Summary: Immobilizing GAPDH onto Fe3O4 magnetic nanoparticles enhanced its properties in histamine degradation, shifting optimal pH and temperature and broadening pH tolerance and thermostability. The immobilized enzyme was efficient in removing histamine in fermented beverages, showing potential for practical applications.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2021)
Article
Agricultural Engineering
Zhemin Liu, Chen Ning, Mingxue Yuan, Suxiao Yang, Xinyi Wei, Mengshi Xiao, Xiaodan Fu, Changliang Zhu, Haijin Mou
BIORESOURCE TECHNOLOGY
(2020)
Article
Chemistry, Applied
Xiaodan Fu, Xinyi Wei, Mengshi Xiao, Zhenlian Han, Francesco Secundo, Haijin Mou
CARBOHYDRATE POLYMERS
(2020)
Article
Fisheries
Shuang Wang, Qing Kong, Petcharat Namwong, Peng Wang, Haijin Mou, Prapansak Srisapoome
Article
Chemistry, Applied
Shanquan Liang, Tan Zhang, Xiaodan Fu, Changliang Zhu, Haijin Mou
CARBOHYDRATE POLYMERS
(2020)
Article
Chemistry, Applied
Fang He, Qing Kong, Zhenhui Jin, Haijin Mou
CARBOHYDRATE POLYMERS
(2020)
Article
Chemistry, Medicinal
Suxiao Yang, Zhemin Liu, Xiaodan Fu, Changliang Zhu, Qing Kong, Min Yang, Haijin Mou
Article
Biochemical Research Methods
Zhemin Liu, Mingxue Yuan, Xiaoyue Zhang, Qingping Liang, Min Yang, Haijin Mou, Changliang Zhu
PROTEIN EXPRESSION AND PURIFICATION
(2020)
Article
Agricultural Engineering
Zhemin Liu, Chen Ning, Mingxue Yuan, Xiaodan Fu, Suxiao Yang, Xinyi Wei, Mengshi Xiao, Haijin Mou, Changliang Zhu
BIORESOURCE TECHNOLOGY
(2020)
Review
Microbiology
Yuchen Huan, Qing Kong, Haijin Mou, Huaxi Yi
FRONTIERS IN MICROBIOLOGY
(2020)
Article
Chemistry, Applied
Mengshi Xiao, Xiaodan Fu, Xinyi Wei, Yongzhou Chi, Wei Gao, Ying Yu, Zhemin Liu, Changliang Zhu, Haijin Mou
Summary: This study successfully utilized bacteriophage-borne glycanase to degrade FcEPS from Enterobacter sakazakii and obtain disaccharide fragments of FCOs. The FcEPS was shown to be a reliable source of disaccharides, and bacteriophage-borne glycanase was an effective tool for degradation.
CARBOHYDRATE POLYMERS
(2021)
Article
Virology
Mengshi Xiao, Xinmiao Ren, Ying Yu, Han Sun, Haijin Mou, Xiaodan Fu
Summary: In this study, a new species named Cronobacter virus PF-CE2 was characterized, and its encoded enzyme capable of degrading specific substrates was predicted. The taxonomic status of eight other phages was changed based on nucleotide sequence comparisons. This work provides a theoretical basis for heterologous expression and serves as an important reference for preservation and sharing of these phages.
ARCHIVES OF VIROLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Shanquan Liang, Tan Zhang, Zhihao Liu, Jingyu Wang, Changliang Zhu, Qing Kong, Xiaodan Fu, Haijin Mou
Summary: Vibrio parahaemolyticus demonstrates complex survival mechanisms under high hydrostatic pressure, including morphological changes and differential gene expression. Under 20 MPa conditions, genes related to energy metabolism were downregulated, while genes related to adhesion and virulence factors were upregulated, with a response mechanism involving RNA polymerase sigma factor RpoE.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Agricultural Engineering
Yuxin Wang, Jia Wang, Ziqiang Gu, Shufang Yang, Yongjin He, Haijin Mou, Han Sun
Summary: An adaptive laboratory evolution (ALE) strategy was used to transform autotrophic Nitzschia closterium into a mixotrophic strain for increased production of essential amino acid (EAA), eicosapentaenoic acid (EPA), and fucoxanthin. The ALE strategy successfully modified carbon metabolism and improved biomass concentration by 65.07% compared to the wild type. By enhancing NADPH oxidase activity, protein degradation, and carbon flux to TCA cycle, the strategy increased the production of EPA, EAA, and fucoxanthin by 41.0%, 18.8%, and 20.4% respectively. This ALE strategy shows promise for high-value microalgal production.
BIORESOURCE TECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Nannan Li, Xiaodan Fu, Mengshi Xiao, Xinyi Wei, Min Yang, Zhemin Liu, Haijin Mou
Article
Marine & Freshwater Biology
Meng Li, Haijin Mou, Qing Kong, Tan Zhang, Xiaodan Fu
MARINE LIFE SCIENCE & TECHNOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Wei-Han Hui, Yen-Lin Chen, Shu-Wei Chang
Summary: Crosslinking affects the mechanical behavior and tissue metabolism of collagen-based tissues. Aging and diabetes alter the crosslinking type and density, thus changing tissue properties. High-connectivity enzymatic crosslinking and advanced-glycation end-products result in uniform deformation under daily activity, while low-connectivity enzymatic crosslinking does not. High-connectivity model displays more sliding, while AGEs induce instability in structures near the binding sites.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Yao-Bin Ma, Xiao-Qiu Zhou, Wei-Dan Jiang, Pei Wu, Yang Liu, Shu-Wei Li, Ling Tang, Lu Zhang, Hai-Feng Mi, Lin Feng
Summary: Tea polyphenols (TPs) are an inexpensive feed additive that can protect the gill function of fish against Flavobacterium columnare (F. columnare) through various mechanisms, including suppression of oxidative stress, apoptosis, and inflammation.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Yan-Jia Guo, Jing-Ru Pang, Yu Zhang, Zhong-Rui Li, Xiao-Lin Zi, Hong-Min Liu, Ning Wang, Li-Juan Zhao, Ya Gao, Bo Wang, Piet Herdewijn, Cheng-Yun Jin, Ying Liu, Yi-Chao Zheng
Summary: This study identifies the conjugation of NEDD8 to LSD1 and its impact on GC cells, revealing the significance of LSD1 neddylation in GC.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Review
Biochemistry & Molecular Biology
Geum-Jae Jeong, Fazlurrahman Khan, Nazia Tabassum, Young-Mog Kim
Summary: Vibrio species are motile gram-negative bacteria commonly found in aquatic environments. They can adapt to environmental fluctuations and develop protective mechanisms against host immune systems. Different types of sigma and anti-sigma factors play important roles in regulating gene expression and virulence in Vibrio species.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Zahra Mohammadbagheri, Behrooz Movahedi, Shaghayegh Saeedi, Abbas Rahmati
Summary: In this study, a reusable biobased composite hydrogel was successfully synthesized and used for the removal of Tl(I) from aqueous solutions. The hydrogel exhibited high adsorption capacity and thermal stability, and the adsorption process was found to be thermodynamically spontaneous. The results demonstrated that the hydrogel can be reused multiple times without significant loss of its adsorption capacity.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Iqra Batool, Muhammad Imran, Ayesha Anwar, Farhan Ahmed Khan, Afrah E. Mohammed, Ashwag Shami, Hafiz Iqbal
Summary: Enzyme immobilization on ZnO/SnO2 and ZnO/SnO2/SA nanocomposites was studied, and the catalytic and biochemical characteristics of the immobilized enzyme were investigated. The results showed that ZnO/SnO2 and ZnO/SnO2/SA nanostructures are suitable carriers for enzyme immobilization.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Elias Mosaffa, Dhruvi Patel, Nasim Amiri Ramsheh, Rishikumar Indravadan Patel, Atanu Banerjee, Hossein Ghafuri
Summary: In this study, a unique 3D bead structure of adsorbent, composed of bacterial cellulose microfilaments reinforced chitosan adorned with melamine 2D plates, was synthesized for anionic dye removal. The adsorbent showed high specific surface area and abundant functional groups, resulting in exceptional adsorption capacity. The characteristics and optimal conditions of the adsorbent were assessed and analyzed.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Carolina Gismene, Jorge Enrique Hernandez Gonzalez, Marilia de Freitas Calmon, Andrey Fabricio Ziem Nascimento, Angela Rocio Nino Santisteban, Felipe Antunes Calil, Alana Della Torre da Silva, Paula Rahal, Rejane Maira Goes, Raghuvir Krishnaswamy Arni, Ricardo Barros Mariutti
Summary: Mammaliicoccus sciuri, a bacterium of clinical and veterinary relevance, expresses the virulence factor Exfoliative Toxin C (ExhC), which binds specifically to desmoglein-1 (Dsg1) and plays a role in tissue integrity. This study investigates the necrotic activity of ExhC and identifies a variant lacking necrotic potential but retaining enzymatic activity through mutagenesis experiments and crystal structure determination.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Bibhas K. Bhunia, Ashutosh Bandyopadhyay, Souradeep Dey, Biman B. Mandal
Summary: Intervertebral disc degeneration is a major cause of chronic low back pain and disability. Researchers have developed a bioactive silk-based hybrid hydrogel system functionalized with decellularized human Wharton's jelly extracellular matrix as bioactive cues. The hydrogels mimic the rheological and mechanical properties of the native nucleus pulposus tissue and support cell viability, proliferation, and tissue maturation.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Qun Zhang, Yonggan Yan, Zhao Li, Jing Du, Kai Zhang, Liguo Zhang, Ting Wang, Alberto Bianco, Shaohua Ge, Baojin Ma
Summary: A new crosslinking strategy has been devised to construct injectable sodium alginate hydrogels, which can be regulated for injectability and have great potential in biomedical applications.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Seong Eun Hong, Ji-Soo Lee, Hyeon Gyu Lee
Summary: The potential of multi-layer nanoemulsions for improving the cellular uptake, antioxidant activity, and in vitro bioaccessibility of alpha-tocopherol was examined. The results showed that the multi-layer nanoemulsions significantly enhanced the cellular uptake and antioxidant activity of alpha-tocopherol compared to free coumarin 6. Furthermore, the use of chitosan and dextran sulfate as wall materials in the multi-layer nanoemulsions led to even higher cellular uptake, in vitro bioaccessibility, and antioxidant activity of alpha-tocopherol.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Jingjing Yu, Yanhui Zhang, Ruoning Zhang, Yanxiang Gao, Like Mao
Summary: High internal phase emulsions (HIPEs) based on beeswax (BW) oleogels and octenyl succinic acid starch (OSA starch) were prepared by a simple one-step method. The results showed that oleogelation improved the stability and encapsulation capability of the HIPEs, which is significant for the development of novel healthy food.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Review
Biochemistry & Molecular Biology
Bi-Feng Yao, Xiu-Ju Luo, Jun Peng
Summary: The balanced and continuous fission and fusion of mitochondria, regulated by OPA1, are important for maintaining mitochondrial dynamics in cardiovascular disorders. Understanding the role of OPA1 in mitochondrial fusion can provide insights into the pathogenesis of cardiovascular diseases.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Review
Biochemistry & Molecular Biology
Gokul Paramasivam, Anandhavelu Sanmugam, Vishnu Vardhan Palem, Murugan Sevanan, Ananda Babu Sairam, Nachiappan Nachiappan, Buhyun Youn, Jung Sub Lee, Muthuchamy Nallal, Kang Hyun Park
Summary: Nanomaterials are being used to deliver therapeutic agents in living systems. Different nanotechnological applications such as immunosensors, immune assays, and drug delivery are being implemented using nanotechnology. Genomics plays a crucial role in selecting appropriate drugs for personalized cancer therapy. It helps in analyzing gene and protein expression, disease classification, and designing disease models. Delivering drugs to specific areas using nanoparticles is a challenging issue.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Jing Li, Wei Wang, Han Wu, Feng Peng, Hui Gao, Ying Guan
Summary: In this study, CMH-based CPF films were prepared and their thermal and barrier properties were successfully regulated. The tensile strength and water vapor barrier property of CPF films were significantly improved, making them promising for food packaging applications.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)