Article
Plant Sciences
Biljana Todorovic, Veno Jasa Grujic, Andreja Urbanek Krajnc, Roman Kranvogl, Jana Ambrozic-Dolinsek
Summary: The study aimed to determine the content of astaxanthin and its esterified forms in Haematococcus pluvialis samples, identified eleven astaxanthin monoesters, six astaxanthin diesters, and found that all three solvents (MTBE, HEX-IPA, ACE) were effective for extraction, with MTBE and hexane-isopropanol extracting the most astaxanthin.
Review
Agricultural Engineering
Bolam Kim, Soo Youn Lee, Aditya Lakshmi Narasimhan, Sangui Kim, You-Kwan Oh
Summary: The green microalga Haematococcus pluvialis is a valuable source of astaxanthin, a powerful antioxidant widely used in various industries. The unique cyst-like cell with a rigid three-layered cell wall formed during astaxanthin accumulation presents challenges for extraction. Various cell disruption methods and downstream processes for sustainable development are also discussed in this review.
BIORESOURCE TECHNOLOGY
(2022)
Review
Agricultural Engineering
Yuanyua Ren, Jinquan Deng, Junchao Huang, Zhaoming Wu, Lanbo Yi, Yuge Bi, Feng Chen
Summary: Astaxanthin, an important antioxidant and nutraceutical compound, is widely used in dietary supplements and cosmetics. The refinery of astaxanthin from Haematococcus pluvialis is a growing industrial sector, as it can accumulate astaxanthin up to 5% of dry weight. Further research on production optimization and joint-production with lipids could make algal-derived astaxanthin more accessible for various applications.
BIORESOURCE TECHNOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Petras Venckus, Ieva Endriukaityte, Kotryna Cekuolyte, Renata Gudiukaite, Andrius Pakalniskis, Egle Lastauskiene
Summary: This study tested the effects of silver nanoparticles on Haematococcus pluvialis and found that high concentrations of AgNPs significantly reduced culture productivity in green-stage cultures, while the impact on red-stage cultures was smaller. The addition of AgNPs also led to changes in cell morphology, including deformations, disrupted cytokinesis, and induced sexual reproduction.
Article
Agricultural Engineering
Aye Aye Myint, Patrick Hariyanto, Muhammad Irshad, Cao Ruqian, Sabrinna Wulandari, Min Eui Hong, Sang Jun Sim, Jaehoon Kim
Summary: A novel integrated extraction technique was demonstrated to achieve high recovery of natural astaxanthin from wet encysted Haematococcus pluvialis without the need for a pre-drying step. By researching the most suitable green solvent and optimizing the extraction conditions, a high recovery of astaxanthin and extraction yield were achieved. The biphasic solvent system played a key role in this process.
BIORESOURCE TECHNOLOGY
(2022)
Article
Green & Sustainable Science & Technology
Gannoru Kankanamalage Sanuji Hasara Nishshanka, Vinoj Chamilka Liyanaarachchi, Pemaththu Hewa Viraj Nimarshana, Thilini Udayangani Ariyadasa, Jo-Shu Chang
Summary: The microalga Haematococcus pluvialis has high biotechnological potential for producing various valuable compounds. Biorefining, which integrates multiple production processes in a single facility, can maximize the utilization of feedstock and reduce resource consumption. This review discusses the potential of biorefining H. pluvialis to promote more sustainable and cleaner production technologies.
JOURNAL OF CLEANER PRODUCTION
(2022)
Article
Chemistry, Applied
Walter Pitacco, Chiara Samori, Laura Pezzolesi, Virginia Gori, Antonio Grillo, Matteo Tiecco, Martina Vagnoni, Paola Galletti
Summary: Three novel hydrophobic deep eutectic solvents (DESs) based on oleic acid and terpenes were prepared and used to extract astaxanthin. The DESs showed high efficiency in extracting astaxanthin and their chemical composition was safe and edible. The carotenoid profile obtained using DESs was similar to that obtained using traditional organic solvents. The thymol:oleic acid DES (TAO) exhibited superior antioxidant properties and could preserve astaxanthin content after light exposure, making it a promising formulation for the food ingredients/additives industry.
Article
Agricultural Engineering
Yongteng Zhao, Hui-Ping Wang, Chunli Yu, Wei Ding, Benyong Han, Shuxiang Geng, Delu Ning, Ting Ma, Xuya Yu
Summary: The study revealed that melatonin treatment upregulated the biosynthesis of astaxanthin and lipids in Haematococcus pluvialis, affecting the accumulation process through the regulation of gene expression and metabolic pathways. The treatment may promote coproduction of astaxanthin and lipids by regulating the carbon-nitrogen balance and antioxidant system.
BIORESOURCE TECHNOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Adila Gherabli, Nabil Grimi, Julien Lemaire, Eugene Vorobiev, Nikolai Lebovka
Summary: The freshwater microalga Haematococcus pluvialis is a cell factory for natural astaxanthin, with 4-7% of its total dry weight composed of astaxanthin. The bioaccumulation of astaxanthin in H. pluvialis cysts is a complex process dependent on different stress conditions during cultivation. This review analyzes the various steps in H. pluvialis' processing, including cultivation, biomass harvesting, cell disruption, extraction, and purification techniques. It also collects information on cell structure, biomolecular composition, properties, and the bioactivity of astaxanthin in H. pluvialis, with special emphasis on the recent progress in applying electrotechnologies for growth and biomolecule recovery.
Article
Biotechnology & Applied Microbiology
Henrique Vechio, Andre Bellin Mariano, Rafael Bruno Vieira
Summary: The study investigated the extraction of astaxanthin from Haematococcus pluvialis using acid hydrolysis and solid-liquid extraction, finding that the optimal conditions with hydrochloric acid and acetone resulted in a high recovery rate of 99%. Even under optimal conditions, acid hydrolysis did not completely disrupt the cell wall, indicating that solvent accessibility occurred through microperforations.
JOURNAL OF APPLIED PHYCOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Jae Been Lee, Yun Hwan Park, Min Seo Jeon, Sok Kim, Yoon-E Choi
Summary: In this study, the researchers successfully increased the production of astaxanthin in Haematococcus pluvialis by combining polyethylenimine (PEI) with chitosan. The polyethylenimine concentration had an effect on astaxanthin accumulation, while the molecular weight of chitosan did not. The treatment also increased the reactive oxygen species content in cells, leading to the upregulation of enzymes involved in astaxanthin biosynthesis. The PEI-chitosan fiber treatment showed high recyclability and maintained over 90% astaxanthin production efficiency.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Agricultural Engineering
Hang Guo, Tao Li, Yongteng Zhao, Xuya Yu
Summary: Copper treatment significantly increased astaxanthin and lipid synthesis in Haematococcus pluvialis, leading to upregulation of biosynthesis genes and antioxidant enzyme-related genes, as well as a decrease in reactive oxygen species levels. Additionally, copper induced the activation of protein kinases, facilitating the production of astaxanthin and lipids in the algae.
BIORESOURCE TECHNOLOGY
(2021)
Article
Engineering, Environmental
Kuan Shiong Khoo, Chien Wei Ooi, Kit Wayne Chew, Su Chern Foo, Jun Wei Lim, Yang Tao, Ning Jiang, Shih-Hsin Ho, Pau Loke Show
Summary: This study successfully extracted astaxanthin from H. pluvialis microalgae using CO2-based alkyl carbamate ILs, with DIMCARB showing the best performance. The optimized conditions resulted in a high yield of astaxanthin and the extracted compound displayed good antioxidant capacity. The distillable IL used in this extraction process proved to be suitable for extracting phytonutrients from algae sources.
CHEMICAL ENGINEERING JOURNAL
(2021)
Article
Chemistry, Multidisciplinary
Andreas Bauer, Mirjana Minceva
Summary: The microalgae Haematococcus pluvialis is used for biotechnological production of astaxanthin, with extraction achieved by germination of cyst cells instead of mechanical disruption, improving extraction efficiency.
APPLIED SCIENCES-BASEL
(2021)
Article
Agricultural Engineering
Hailiang Xing, Yongteng Zhao, Tao Li, Benyong Han, Peng Zhao, Xuya Yu
Summary: The combination of melatonin and putrescine enhances the production of astaxanthin (Asta) and lipids in Haematococcus pluvialis, increasing endogenous putrescine content and reducing oxidative stress levels. This strategy also upregulates carotenogenic, lipogenic, and antioxidant enzyme genes in the microalga.
BIORESOURCE TECHNOLOGY
(2022)
Article
Engineering, Biomedical
Anne Meddahi-Pelle, Graciela Pavon-Djavid, Nabil Chakfe, Frederic Heim
Summary: Transcatheter Aortic Valve Implantation (TAVI) has become a popular alternative to surgical valve replacement, but there is limited knowledge about the long-term durability of transcatheter implanted biological tissue. A preliminary animal case study showed that woven fabric valve leaflets made from 45-degree inclined yarns could limit the development of fibrosis.
BIOMEDICAL ENGINEERING-BIOMEDIZINISCHE TECHNIK
(2021)
Article
Chemistry, Multidisciplinary
Paulette Gomez-Lopez, Jose Angel Salatti-Dorado, Daily Rodriguez-Padron, Manuel Cano, Clemente G. Alvarado-Beltran, Alain R. Puente-Santiago, Juan J. Giner-Casares, Rafael Luque
Summary: A new class of PAN-based Co-N-doped carbon materials was reported as efficient catalysts for OER. The study found that Co doping concentration and treatment conditions affected the catalytic performance, with Co-N-doped carbon materials exhibiting good OER catalytic performance in alkaline environments.
Article
Chemistry, Physical
Juan Amaro-Gahete, Jose A. Salatti-Dorado, Almudena Benitez, Dolores Esquivel, Valentin Garcia-Caballero, Miguel Lopez-Haro, Juan J. Delgado, Manuel Cano, Juan J. Giner-Casares, Francisco J. Romero-Salguero
Summary: The assembly of atomically dispersed iron-nitrogen sites into graphitic structures is a promising approach for sustainable production of bifunctional electrocatalysts. A novel low-temperature and solvent-free mechanochemical synthesis method allows for precise engineering of these active sites at the edges of graphene.
SUSTAINABLE ENERGY & FUELS
(2022)
Article
Biochemistry & Molecular Biology
Abolfazl Barzegari, Yadollah Omidi, Rebecca Landon, Virginie Gueguen, Sepideh Parvizpour, Anne Meddahi-Pelle, Fani Anagnostou, Graciela Pavon-Djavid
Summary: Transplantation of mesenchymal stem cells (MSCs) is an effective treatment for tissue injuries, but it is limited by early cell death. This study found that oxidative stress and energy metabolism disruption are the main reasons for the early death of MSCs. Strategies such as preconditioning and antioxidant pretreatment are being developed to improve the bio-functional properties of MSCs. The use of N-acetylcysteine (NAC) was shown to alleviate DNA damage, cell apoptosis, and oxidative stress, but did not affect mitochondrial membrane potential.
CHEMICO-BIOLOGICAL INTERACTIONS
(2022)
Article
Chemistry, Analytical
S. Gonzalez-Rubio, A. Ballesteros-Gomez, D. Garcia-Gomez, S. Rubio
Summary: SUPRASs with increased hydrophilicity were synthesized to enhance the extractability of polar compounds. The aggregates formed by self-assembled double-headed amphiphile in hydro-organic media showed highly hydrophilic microenvironments, leading to increased recovery factors for perfluorinated compounds in natural waters. This work demonstrates that hydrophilicity in SUPRASs can be tailored to improve compound extractability in multi-residue analysis.
Article
Biochemistry & Molecular Biology
Rosa Calderon-Jacinto, Pietro Matricardi, Virginie Gueguen, Graciela Pavon-Djavid, Emmanuel Pauthe, Violeta Rodriguez-Ruiz
Summary: This study focuses on the development and evaluation of a dual nanostructured lipid carrier (NLC)/Carbopol(R)-based hydrogel system for the topical delivery of curcumin to the skin. The results demonstrate that CUR-NLCs have high curcumin antioxidant activity and moderate anti-migration/proliferation effect. The dual CUR-NLC/hydrogel system shows potential application in adjuvant treatment for topical dermatological applications.
Review
Cell Biology
Abolfazl Barzegari, Sobhan Aaboulhassanzadeh, Rebecca Landon, Virginie Gueguen, Anne Meddahi-Pelle, Sepideh Parvizpour, Fani Anagnostou, Graciela Pavon-Djavid
Summary: This review provides an update on the broader concept of stem cell fate in stress conditions of damaged tissues, and highlights the importance of mitochondrial hormesis in the control of stem cell programming for successful clinical cell therapy.
JOURNAL OF CELLULAR PHYSIOLOGY
(2022)
Article
Chemistry, Analytical
Kim Arnold, Philippe Dehio, Jonas Lotscher, Kapil Dev Singh, Diego Garcia-Gomez, Christoph Hess, Pablo Sinues, Maria L. Balmer
Summary: In this study, a novel setup coupled with a secondary electrospray ionization-high resolution mass spectrometric (SESI-HRMS) platform was developed to analyze the real-time metabolic changes in immature and activated dendritic cells (DCs). Distinct metabolic signatures were detected in DCs treated with different supernatants of bacterial cultures, and differences in the metabolic profile of naive and activated DCs were discovered. Pathway-enrichment analysis revealed three significantly altered pathways.
ANALYTICAL CHEMISTRY
(2023)
Article
Biochemical Research Methods
Mo Awchi, Pablo Sinues, Alexandre N. Datta, Diego Garcia-Gomez, Kapil Dev Singh
Summary: Real-time breath analysis using secondary electro-spray ionization coupled with high-resolution mass spectrometry is a fast and noninvasive method to assess a person's metabolic state. However, the inability to confidently assign mass spectral features to compounds due to the lack of chromatographic separation can be overcome by using exhaled breath condensate and conventional LC-MS systems. In this study, the presence of six amino acids (GABA, Oxo-Pro, Asp, Gln, Glu, and Tyr), which have been reported to be involved in response to antiseizure medications and their side effects, was confirmed in exhaled breath condensate and, by extension, in exhaled human breath for the first time. Raw data is publicly available at MetaboLights with the accession number MTBLS6760.
JOURNAL OF PROTEOME RESEARCH
(2023)
Review
Cell Biology
Seyyedeh Mina Hejazian, Mohammadreza Ardalan, Seyed Mahdi Hosseiniyan Khatibi, Yalda Rahbar Saadat, Abolfazl Barzegari, Virginie Gueguen, Anne Meddahi-Pelle, Fani Anagnostou, Sepideh Zununi Vahed, Graciela Pavon-Djavid
Summary: Podocytes are important kidney cells that prevent proteinuria by acting as gatekeepers of the glomerular filtration barrier. Podocytopathies are kidney diseases caused by damage to these cells, which can be caused by various genetic and environmental factors. Recent evidence suggests that mitochondrial dysfunction plays a role in podocyte damage, and further research is needed to understand the underlying mechanisms and potential therapeutic targets.
JOURNAL OF CELLULAR PHYSIOLOGY
(2023)
Article
Food Science & Technology
Carlos Adelantado, Jose Angel Salatti-Dorado, Carlos Herrera, Lourdes Arce, Maria Jose Cardador
Summary: High-performance-ion mobility spectrometry (HPIMS) was used for the first time in the analysis of olive oil samples, following a simple liquid-liquid extraction procedure. The developed methodology is user-friendly, fast, and inexpensive, complying with the principles of green chemistry. The proposed approach, combined with multivariate analysis, showed potential in classifying olive oil samples based on their organoleptic quality, with high accuracy rates.
Meeting Abstract
Cell & Tissue Engineering
Rebecca Landon, Abolfazl Barzegari, Nathanael Larochette, Virginie Gueguen, Herve Petite, Graciela Pavon-Djavid, Fani Anagnostou
TISSUE ENGINEERING PART A
(2022)
Article
Chemistry, Physical
Ana Franco, Jose Angel Salatti-Dorado, Valentin Garcia-Caballero, Sebastian Lorca, Luis Camacho, Manuel Cano, Antonio J. Fernandez-Romero, Juan J. Delgado, Juan J. Giner-Casares, Carolina Carrillo-Carrion
Summary: A new ultrathin and water-stable 2D Cu-based metal-organic framework has been synthesized with notable electrocatalytic activity for the oxygen reduction reaction in alkaline media, showing excellent performance in both alkaline flooded and all-solid-state Zn-air batteries.
JOURNAL OF MATERIALS CHEMISTRY A
(2022)
Meeting Abstract
Clinical Neurology
K. D. Singh, M. Osswald, V. C. Ziesenitz, M. Awchi, J. Usemann, L. L. Imbach, M. Kohler, D. Garcia-Gomez, C. Zwicky, Jvd Anker, U. Frey, A. N. Datta, P. Sinues
Article
Chemistry, Applied
M. Graca Dias, Elsa Vasco, Francisco Ravasco, Lufsa Oliveira
Summary: This study estimated the vitamin D intake of "adults" and "elderly" populations in Portugal using the TDS methodology. The results showed that the majority of people had inadequate vitamin D intake, well below the Dietary Reference Values.
Article
Chemistry, Applied
Yanan Wang, Jiachen Shi, Yong-Jiang Xu, Chin-Ping Tan, Yuanfa Liu
Summary: This study investigates the variations in lipid digestion profiles among individuals of different ages using in vitro digestion models. The findings suggest that adults have a more comprehensive lipid digestion compared to infants, and infants tend to release shorter chain length and more saturated free fatty acids during digestion. Additionally, the particle sizes in the stomach of the elderly were consistently larger. This study enhances our understanding of how lipids with different degrees of unsaturation undergo digestion in diverse age groups.
Article
Chemistry, Applied
Hyunjong Yu, Huisu Kim, Pahn-Shick Chang
Summary: Chitosan-pectin emulsion-filled hydrogel (EFH) was developed to enhance the bioaccessibility of lipophilic bioactive compounds through intestinal delivery. The EFH, prepared without crosslinking agents, demonstrated improved mechanical strength and compactness with higher pectin concentration. It retained the emulsion at pH 2.0 and released it at pH 7.4, resulting in enhanced release of free fatty acids and improved bioaccessibility of curcumin.
Article
Chemistry, Applied
Tongze Zhang, Siqi Hong, Jia-Rong Zhang, Pin-He Liu, Siyi Li, Zixian Wen, Jianwei Xiao, Guirong Zhang, Olivier Habimana, Nagendra P. Shah, Zhongquan Sui, Harold Corke
Summary: Lactic acid fermentation significantly affects the morphology and physicochemical properties of proso millet starch, including the formation of surface indentations and small pores, decrease in gelatinization temperatures, and changes in hardness and adhesiveness.
Article
Chemistry, Applied
Liqin Kong, Feng Hong, Peng Luan, Yiping Chen, Yaoze Feng, Ming Zhu
Summary: This study presents a novel impedance biosensor using composite nanomaterials and T1R1 as a signal probe, which can competitively and ultra-sensitively detect umami intensity. The biosensor exhibits exceptional analytical performance and is suitable for food flavor evaluation.
Article
Chemistry, Applied
Kunying Lv, Yixin Yang, Qilong Li, Ran Chen, Liang Deng, Yiwei Zhang, Ning Jiang
Summary: Horse's milk, with its high nutritional value and low allergenic proteins, could be a substitute for cow's milk in infant consumption. A proteomic method was used to identify and compare milk fat globule membrane (MFGM) and whey proteins from different horse breeds. The study found differences in protein composition and functionality, which could support the development of formulas more suitable for human infants.
Article
Chemistry, Applied
Enrique Jacobo Diaz-Montana, Helene Brignot, Ramon Aparicio-Ruiz, Thierry Thomas- Danguin, Maria Teresa Morales
Summary: Sensory perception of virgin olive oil is influenced by phenols and volatiles, which are affected by the composition of the oil and biological factors. This study investigated the effect of saliva and phenols on the release of volatiles, and found that the presence of phenols decreased the release of saturated volatiles.
Article
Chemistry, Applied
Wei Zhou, Rui Zhang, Zhen Cai, Fangfang Wu, Yong Hu, Chao Huang, Kun Hu, Yun Chen
Summary: Environmentally friendly and outstanding pH-responsive cationic starch nanoparticles (CSNP) were prepared from pH-sensitive starch. CSNP exhibited nanosize and regular sphere, highly free-flowing molecular chains, and demonstrated excellent pH responsiveness through multiple emulsion/demulsification transitions.
Article
Chemistry, Applied
Andrea Koo, Vinayak Ghate, Weibiao Zhou
Summary: This study suggests that direct seeding may negatively affect the nutritional quality of crops, causing a decrease in ascorbic acid, vitamin K, and total glucosinolate content.
Article
Chemistry, Applied
Tonghao Du, Yazhou Xu, Xiaoyan Xu, Shijin Xiong, Linli Zhang, Biao Dong, Jinqing Huang, Tao Huang, Muyan Xiao, Tao Xiong, Mingyong Xie
Summary: This study successfully improved the ACE inhibitory activity of black sesame seeds by fermenting them with Lactobacillus Plantarum NCU116 and hydrolyzing them using acid protease. The RF-PSO model was used to predict the ACE inhibitory activity during the hydrolysis process. Eight peptides with ACE inhibitory activity were identified from fermented black sesame seed hydrolysates after separation and screening.
Article
Chemistry, Applied
Yao Liu, Songyi Lin, Kexin Liu, Shan Wang, Qiaozhen Liu, Na Sun
Summary: This study analyzed the structural changes of shrimp proteins during digestion, predicted the immunodominant epitopes, and validated their allergenicity. The results showed that shrimp proteins were degraded into peptides during digestion, but still carried IgE epitopes that trigger allergic reactions.
Article
Chemistry, Applied
Tiantian Fu, Hongwei Cao, Yu Zhang, Xiao Guan
Summary: This study investigates the impact of milling on the active components in rice, with a focus on the stability and bioaccessibility of phenols, VB1, and alpha-GABA during cooking and digestion. The findings show that milling exacerbates the instability of gamma-GABA during cooking and VB1 during digestion, and it affects the bioaccessibility of these active compounds.
Article
Chemistry, Applied
Zhihao Yang, Yanru Hou, Min Zhang, Puxin Hou, Chang Liu, Lu Dou, Xiaoyu Chen, Lihua Zhao, Lin Su, Ye Jin
Summary: This study investigated the molecular mechanism of feeding regimes on lamb flavor by using TMT labeling combined with MS. The results showed that pasture-fed groups had higher levels of amino acids and volatile flavor substances compared to concentrate-fed groups. Additionally, several differentially abundant proteins associated with lamb flavor were identified.
Article
Chemistry, Applied
Zixuan Xie, De Zhang, Junyu Zhu, Qianqian Luo, Jun Liu, Jingtao Zhou, Xiaoyong Wang, Yuqiong Chen, Zhi Yu, Dejiang Ni
Summary: This study investigated the acidification of aroma-enhanced black tea during storage. Analysis of non-volatile substances and organic acids using UPLC-Q-TOF/MS and HPLC revealed a decrease in soluble sugars and amino acids, while an increase in organic acids such as oxalic acid, malic acid, and quinic acid. In vitro experiments further demonstrated that the acidification is a result of the decomposition of sugars and amino acids by heating, as well as the oxidation of aromatic aldehydes. Additionally, the study showed that the taste composition of tea infusion is altered, with reduced amino acids, catechins, soluble sugars, and flavonoids. This research provides a theoretical basis for improving the quality of black tea.
Article
Chemistry, Applied
Lin Wang, Falai Ma, Zihan Li, Yan Zhang
Summary: This study developed time-temperature integrators based on amyloglucosidase@Cu3(PO4)2 nanoflowers for monitoring the freshness of chilled pork. The results showed that the integrators were highly reliable and accurate in predicting the quality of chilled pork.
