Article
Biochemistry & Molecular Biology
Curro Polo-Castellano, Rosa Maria Mateos, Francisco Visiedo, Miguel Palma, Gerardo F. Barbero, Marta Ferreiro-Gonzalez
Summary: Moringa oleifera Lam. is a tree with significant antioxidant properties, making the development of an optimal extraction method crucial for obtaining pharmacological products based on its bioactive compounds. Enzymatic extraction conditions were optimized using Plackett-Burman and Box-Behnken designs, with normalized areas and relative mass as response and quantification variables, respectively. Under the optimal extraction conditions, enzyme extractions were performed and evaluated for antioxidant power, total phenolic compounds, repeatability, and intermediate precision.
Review
Plant Sciences
Mohamed Ahmed Hassan, Tao Xu, Yang Tian, Yongheng Zhong, Fatma Abo Zakaib Ali, Xuan Yang, Baiyi Lu
Summary: Moringa oleifera, a plant native to India, contains a variety of phenolic compounds that have shown potential therapeutic properties against a range of diseases, including neurodegenerative disorders, kidney damage, diabetes, and hypertension. More research is needed to explore the full potential of these compounds for drug development and disease treatment.
Article
Food Science & Technology
Angela Fernandes, Angela Liberal, Jose Pinela, Tiane C. Finimundy, Aducabe Bancessi, Ana Ciric, Marina Sokovic, Luis Catarino, Isabel C. F. R. Ferreira, Lillian Barros
Summary: Moringa oleifera leaves are rich in nutrients and bioactive substances, with commercially acquired leaves showing better quality profiles and wild-collected leaves exhibiting stronger antibacterial and antifungal activities.
Article
Agronomy
Beatriz Juliana Yerena-Prieto, Monserrat Gonzalez-Gonzalez, Miguel Angel Garcia-Alvarado, Lourdes Casas, Miguel Palma, Guadalupe Del Carmen Rodriguez-Jimenes, Gerardo F. Barbero, Cristina Cejudo-Bastante
Summary: Supercritical fluid extraction (SFE) using CO2 is a sustainable and efficient technique for recovering highly apolar compounds. However, the recovery of phenolic compounds requires the use of different co-solvent combinations and optimization of various variables. Pressure and temperature significantly affect the phenolic composition and concentration of the extracts.
Article
Biochemical Research Methods
Naima Bennour, Hedi Mighri, Talel Bouhamda, Mahmoud Mabrouk, Elif Apohan, Ozfer Yesilada, Hasan Kucukbay, Ahmed Akrout
Summary: The study evaluated the effect of extraction methods and solvents on phenolic composition and biological activities of Moringa oleifera leaves in Tunisia. Results showed that the But extract prepared by successive maceration method exhibited the highest total polyphenol content and strongest antioxidant, antibacterial, and cytotoxic activities, containing the most rutin, quercetin, and syringic acid.
PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Tatiane Luiza Cadorin Oldoni, Suelen dos Santos, Marina Leite Mitterer-Daltoe, Luiz Henrique Iaguszeski Pizone, Vanderlei Aparecido de Lima
Summary: This study evaluated the influence of seasonality, growing area, and regrowth age on the antioxidant potential and biochemical markers of Moringa oleifera leaves. The results showed that all parameters were affected by these factors. Samples cultivated in the southeast had higher phenolic compound content and antioxidant activity. Young leaves had higher antioxidant potential and phenolic content.
ARABIAN JOURNAL OF CHEMISTRY
(2022)
Article
Agriculture, Multidisciplinary
Xiangyu Bian, Yawen Wang, Renren Yang, Yuying Ma, Weiyun Dong, Changjiang Guo, Weina Gao
Summary: This study evaluated the anti-fatigue effect of the ethanol extract of Moringa oleifera leaves (MLEE) on mice and its primary mechanism of action. The results showed that MLEE supplementation increased glucose and non-esterified fatty acids levels while decreasing lactate and blood urea nitrogen levels in serum. It also increased glycogen levels, glycogen synthase activity, and non-esterified fatty acids levels in the muscle. Furthermore, MLEE exerted its anti-fatigue effect via the AMPK-linked route and enhanced antioxidant enzyme activity.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2023)
Article
Chemistry, Analytical
Lihua Xu, Tianyu Ma, Xinming Wang, Wei Liu, Jia Li, Xiao Wang
Summary: The combination of pH-ZRCCC and HSCCC effectively isolated several polyphenols from Perillae Folium, demonstrating its efficiency in preparing and separating polyphenols from natural products, particularly complex mixtures of phenolic acids and flavonoids.
ANALYTICAL METHODS
(2021)
Article
Engineering, Chemical
L. A. Vazquez-Leon, J. A. Olguin-Rojas, D. E. Paramo-Calderon, Gerardo F. Barbero, M. A. Salgado-Cervantes, Miguel Palma, M. A. Garcia-Alvarado, G. C. Rodriguez-Jimenes
Summary: The study investigated the retention of different nutrients in dried moringa extract using different carrier materials during spray drying. Maltodextrin DE-10 showed the highest retention of phenolics and ascorbic acid, while precipitated silica exhibited the highest retention of total carotenoids.
Review
Food Science & Technology
Oana Lelia Pop, Andreea Diana Kerezsi, Calina Ciont (Nagy)
Summary: This review describes the main processes for extracting and encapsulating bioactive compounds from Moringa oleifera and their potential impact on food science and public health. Different extraction techniques significantly impact the molecular structure and biological activity of Moringa polysaccharides, while encapsulation can reduce oxidative stability and deliver active agents into foods.
Article
Multidisciplinary Sciences
Raneem F. Obeid, Mohamed M. Ammar, Sara H. Younis
Summary: This study evaluated the biomimetic remineralization capabilities of Moringa oleifera leaves extract on dentin and cementum. The results showed that the extract had a significant effect on remineralization, filling in some tubules and fissures, and had the potential to control dentin hypersensitivity and form biomimetic cementum tissue.
SCIENTIFIC REPORTS
(2023)
Article
Biotechnology & Applied Microbiology
M. Kashaninejad, B. Blanco, O. Benito-Roman, S. Beltran, S. Mehdi Niknam, M. T. Sanz
Summary: A study on the chemical characterization of Moringa oleifera leaves found that a 50% hydroalcoholic mixture led to the highest yield of total phenolic compounds through conventional solvent extraction. Ultrasound assisted extraction did not improve the extraction process. Water had the highest retention index, which decreased with increasing ethanol concentration.
FOOD AND BIOPRODUCTS PROCESSING
(2021)
Review
Biochemistry & Molecular Biology
Kanika Sharma, Manoj Kumar, Roji Waghmare, Rajat Suhag, Om Prakash Gupta, Jose M. Lorenzo, Suraj Prakash, Radha, Nadeem Rais, Vellaikumar Sampathrajan, Chandan Thappa, T. Anitha, Ali A. S. Sayed, Basel A. Abdel-Wahab, Marisennayya Senapathy, R. Pandiselvam, Abhijit Dey, Sangram Dhumal, Ryszard Amarowicz, John F. Kennedy
Summary: Moringa oleifera Lam. is a plant with various bioactivities, including polysaccharides and gums. Polysaccharides have been found to possess antihyperlipidemic, anti-diabetic, immunomodulatory, antihypertensive, and gastrointestinal protection functions. The extraction methods can influence the structure and bioactivity of polysaccharides. This review provides updated information on the extraction, purification, structural characterization, bioactivities, and potential applications of moringa polysaccharides.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Biochemistry & Molecular Biology
Saima Muzammil, Jorddy Neves Cruz, Rabia Mumtaz, Ijaz Rasul, Sumreen Hayat, Muhammad Asaf Khan, Arif Muhammad Khan, Muhammad Umar Ijaz, Rafael Rodrigues Lima, Muhammad Zubair
Summary: The delayed healing of wounds among people with diabetes is a severe worldwide problem. Hyperglycemia and increased levels of free radicals are the major inhibiting factors of wound healing in diabetic patients. Plant extracts rich in polyphenols can be an effective agent for wound healing, but optimization of the extraction protocol is needed to ensure efficacy.
Article
Plant Sciences
Amira K. G. Atteya, Aishah N. Albalawi, Hala M. Bayomy, Eman S. Alamri, Esmail A. E. Genaidy
Summary: This study investigated the effects of vermicompost and NPK fertilizers, as well as their combination, on the growth and yield of Moringa oleifera tree under calcareous soil conditions. The results showed that increasing the quantity of vermicompost and implementing NPK fertilization, as well as their combination treatments, significantly enhanced various parameters and yield of the Moringa oleifera tree. However, increasing the level of vermicompost, NPK, and their combination treatments resulted in a decrease in the total phenolic and flavonoid content and antioxidant activity of dry Moringa oleifera leaves.
Article
Environmental Sciences
Frysye Gumansalangi, Jose L. P. Calle, Marta Barea-Sepulveda, Manikharda, Miguel Palma, Lideman, Mohamad Rafi, Andriati Ningrum, Widiastuti Setyaningsih
Summary: Macroalgae are essential ingredients for various industries, producing economically valuable derived products. Their unique compositions can be used as markers for authentication and may vary based on geographical regions. Different analytical techniques were applied to classify and standardize the quality of macroalgae samples from different regions in Indonesia.
Article
Agronomy
Mercedes Vazquez-Espinosa, Maria Alvarez-Romero, Ana V. Gonzalez-de-Peredo, Ana Ruiz-Rodriguez, Marta Ferreiro-Gonzalez, Gerardo F. Barbero, Miguel Palma
Summary: The study aimed to determine the optimal harvest time for Bolilla peppers to maximize their bioactive properties. The maximum capsaicinoid concentration in the pericarp was reached at 30 days post-anthesis, while in the placenta it was registered at 41 days. After that, there was a drastic reduction in capsaicinoid content. Older plants had higher capsaicinoid content in the pericarp, while younger plants had higher content in the placenta, which is economically important.
Article
Biochemistry & Molecular Biology
Curro Polo-Castellano, Rosa Maria Mateos, Francisco Visiedo, Miguel Palma, Gerardo F. Barbero, Marta Ferreiro-Gonzalez
Summary: Moringa oleifera Lam. is a tree with significant antioxidant properties, making the development of an optimal extraction method crucial for obtaining pharmacological products based on its bioactive compounds. Enzymatic extraction conditions were optimized using Plackett-Burman and Box-Behnken designs, with normalized areas and relative mass as response and quantification variables, respectively. Under the optimal extraction conditions, enzyme extractions were performed and evaluated for antioxidant power, total phenolic compounds, repeatability, and intermediate precision.
Article
Engineering, Chemical
E. R. Rodriguez-Castillo, M. A. Garcia-Alvarado, M. Ruiz-Reyes, G. Rebollar-Perez, F. M. Pacheco-Aguirre
Summary: This study investigated the mathematical modeling of a laboratory-scale monopolar alkaline electrolyzer, taking into account the thermodynamics of the water dissociation reaction, the kinetics of the electrode, and the ohmic resistance for hydroxide ions. A practical approach was developed to fit the characteristic curves of each experiment to the experimental data. The model simultaneously modeled the reactor and the process configuration. The model was applied to analyze the performance of electrolyzers with different numbers of electrodes using a 1 M solution of KOH.
BRAZILIAN JOURNAL OF CHEMICAL ENGINEERING
(2023)
Article
Plant Sciences
Antonio Ruano-Gonzalez, Ana A. Pinto, Nuria Chinchilla, Miguel Palma, Gerardo F. Barbero, Ceferino Carrera, Mercedes Vazquez-Espinosa
Summary: This study identified the major phenolic compounds in tagarnina, including 5-caffeoylquinic acid (5-CQA) and 3,5-dicaffeoylquinic acid (3,5-diCQA). An ultrasound-assisted extraction method was developed to extract these compounds, with methanol percentage, sample-to-solvent ratio, and pH being the most influential factors. The concentration of 5-CQA and 3,5-diCQA in Scolymus hispanicus collected from six different locations in southern Spain was determined, and the antioxidant activity of the samples was correlated with their caffeoylquinic compound content, showing an antioxidant effect.
Article
Chemistry, Medicinal
Ana V. Gonzalez-de-Peredo, Mercedes Vazquez-Espinosa, Estrella Espada-Bellido, Marta Ferreiro-Gonzalez, Ceferino Carrera, Miguel Palma, Gerardo F. Barbero
Summary: The population is now more aware of the connection between food and general health, and onions are known for their health-promoting properties. This study proposes a method using direct thermal desorption-gas chromatography-mass spectrometry to study the organosulfur compounds in onions. Optimal conditions for pre-extraction and post-analysis were determined, and the repeatability and precision of the method were evaluated. The results showed that 2,4-dimethyl-thiophene was the major compound in onions, while propanethial S-oxide was responsible for the tear factor.
Review
Automation & Control Systems
J. Carrillo-Ahumada, C. Nolasco-Hipolito, G. Reynoso-Meza, I. I. Ruiz-Lopez, M. A. Garcia-Alvarado
Summary: This paper provides a critical review of the tuning methodologies and controller structures applied to the Cholette bioreactor. It discusses various approaches, from controllers with a single structure to nonlinear controllers and from synthesis methods to frequency response. The study identifies new trends and opportunities for research in terms of operating points, controller structures, and tuning methodologies for this system.
Article
Agronomy
Monserrat Gonzalez-Gonzalez, Beatriz Juliana Yerena-Prieto, Ceferino Carrera, Mercedes Vazquez-Espinosa, Ana V. Gonzalez-de-Peredo, Miguel Angel Garcia-Alvarado, Miguel Palma, Guadalupe del Carmen Rodriguez-Jimenes, Gerardo Fernandez Barbero
Summary: This study proposed a microwave-assisted extraction method for the extraction of gingerols and shogaols from ginger rhizomes. Optimum conditions were determined through experimental design and response surface methodology, and the method demonstrated short extraction times and low variation.
Article
Agronomy
Monserrat Gonzalez-Gonzalez, Beatriz Juliana Yerena-Prieto, Ceferino Carrera, Mercedes Vazquez-Espinosa, Ana Velasco Gonzalez-de-Peredo, Miguel Angel Garcia-Alvarado, Miguel Palma, Guadalupe del Carmen Rodriguez-Jimenes, Gerardo Fernandez Barbero
Summary: The goal of this study was to optimize an ultrasound-assisted extraction (UAE) method for extracting the main bioactive compounds (gingerols and shogaols) from ginger rhizome. The optimized method required only 10 minutes of extraction time and showed good repeatability and precision. The method was successfully applied to extract gingerols and shogaols from different ginger samples, demonstrating its applicability and highlighting potential variations in compound concentrations.
Article
Food Science & Technology
Hugo Duarte, Ceferino Carrera, Maria Jose Aliano-Gonzalez, Rocio Gutierrez-Escobar, Maria Jesus Jimenez-Hierro, Miguel Palma, Ludovina Galego, Anabela Romano, Bruno Medronho
Summary: This study explored two effective strategies for the valorisation of Arbutus unedo pomace: optimizing the extraction of polyphenols using ultrasound-assisted method and developing functional cookies with pomace. The results showed that the optimized extraction conditions yielded polyphenol-rich extracts with high antioxidant activity, and the incorporation of pomace in cookies was well-received by consumers.
Article
Food Science & Technology
Maria alvarez-Romero, Ana Ruiz-Rodriguez, Gerardo F. F. Barbero, Mercedes Vazquez-Espinosa, Fouad El-Mansouri, Jamal Brigui, Miguel Palma
Summary: Barley is an important cereal crop used for animal feed and human consumption. Its grains are rich in phenolic compounds, which have health-promoting antioxidant properties. Ultrasound-assisted extraction (UAE) and microwave-assisted extraction (MAE) were optimized and compared to determine the antioxidant power and phenolic compound levels of barley extracts. The solvent composition and its interaction with temperature were found to be the most significant variables. Both methods showed short extraction times, high precision, and good recoveries. Significant differences in phenolic compounds and antioxidant power were observed among different barley varieties, with MAE being slightly more efficient than UAE in terms of phenolic compound extraction.
Article
Food Science & Technology
Marta Barea-Sepulveda, Jose Luis P. Calle, Marta Ferreiro-Gonzalez, Miguel Palma
Summary: In this research, a combination of visible and near-infrared spectroscopy with machine learning algorithms was used to effectively characterize and classify two commonly marketed petroleum waxes. The results showed that hierarchical cluster analysis and principal component analysis could group the wax samples based on their chemical composition. Supported by support vector machines and random forest models, the spectroscopic data enabled precise classification and identification of specific wavelengths for discrimination between wax types.
Article
Food Science & Technology
Jose Luis P. Calle, Irene Punta-Sanchez, Ana Velasco Gonzalez-de-Peredo, Ana Ruiz-Rodriguez, Marta Ferreiro-Gonzalez, Miguel Palma
Summary: This study proposes a method combining visible near infrared spectroscopy (Vis-NIRs) with machine learning algorithms to identify and quantify adulterants in honey. Experimental results showed that samples can be grouped according to botanical origin and the presence of adulteration. Support vector machines (SVM) and random forests (RF) achieved 100% accuracy in detecting adulterations, while support vector regression (SVR) achieved the best result in quantifying the percentage of adulteration.
Article
Horticulture
Deyla Prajna, Maria Alvarez, Marta Barea-Sepulveda, Jose Luis P. Calle, Diding Suhandy, Widiastuti Setyaningsih, Miguel Palma
Summary: Civet coffee, the most expensive and rarest coffee bean in the world, originated from Indonesia. It can be divided into wild and fed types, and the authenticity can be evaluated with high speed and precision using a combination of spectroscopy and chemometrics.
Article
Biotechnology & Applied Microbiology
Mohamad Rais Hasan, Che Azurahanim Che Abdullah, Mustapha Nor Afizah, Mohd Sabri Mohd Ghazali, Mohd Adzahan Noranizan
Summary: This study evaluates the efficacy of ultrasound in the extraction process of calcium hydroxyapatite (HAp). The findings show that low-intensity and moderate-intensity ultrasonication reduce the whiteness index and yield of HAp, while high-intensity ultrasonication reduces particle size and improves crystallinity.
FOOD AND BIOPRODUCTS PROCESSING
(2024)
Article
Biotechnology & Applied Microbiology
Barbara Flaibam, Rosana Goldbeck
Summary: This study evaluated the possibility of extracting proteins and obtaining hydrolysates from non-animal agro-industrial waste using enzymes for the production of cultured meat inputs. Results showed that the application of enzymes can maximize the use of protein from these wastes, providing a low-cost and environmentally friendly route with nutritional potential for cultured meat.
FOOD AND BIOPRODUCTS PROCESSING
(2024)
Article
Biotechnology & Applied Microbiology
Robson Costa de Sousa, Ariany Binda Silva Costa, Marcos Daniel Martins Freitas, Maisa Tonon Bitti Perazzini, Hugo Perazzini
Summary: This study investigated the drying kinetics of black pepper through experimental and mathematical modeling approaches. The models developed provided valuable insights into the coupling between heat and mass transfers during the drying process, and accurately described the drying behavior of black pepper.
FOOD AND BIOPRODUCTS PROCESSING
(2024)
Article
Biotechnology & Applied Microbiology
Ali Nikkhah, Hasan Nikkhah, Amir Shahbazi, Mona Kamelan Zargar Zarin, Duygu Beykal Iz, Mohammad-Taghi Ebadi, Mohammad Fakhroleslam, Burcu Beykal
Summary: Cumin and eucalyptus essential oils were enriched to meet ISO standards using a dynamic fractional distillation process. The optimization results showed low operating costs and potential for improving process efficiency.
FOOD AND BIOPRODUCTS PROCESSING
(2024)
Article
Biotechnology & Applied Microbiology
Sakshi Gupta, Gargi Ghoshal
Summary: A study was conducted to prepare green gram protein hydrogel enriched with curcumin and evaluate its physicochemical properties. The results showed that the hydrogel had high encapsulation efficiency, swelling capacity, and antioxidant property. Furthermore, applying the hydrogel as a coating on tomatoes and apples proved to be effective.
FOOD AND BIOPRODUCTS PROCESSING
(2024)
Article
Biotechnology & Applied Microbiology
Calvin Lo, Rene H. Wijffels, Michel H. M. Eppink
Summary: This study developed a novel strategy to separate dissolved oils from imidazole/hexanoic acid mixture and regenerate the solvent using polar antisolvents. By adding small amounts of water and methanol, high recovery of lipids was achieved, while ethanol required a large amount and caused solvent dilution.
FOOD AND BIOPRODUCTS PROCESSING
(2024)
Article
Biotechnology & Applied Microbiology
Manoj Sawale, Patnarin Benyathiar, Pablo Coronel, Ajay Rawat, Josip Simunovic, Ferhan Ozadali, Dharmendra K. Mishra
Summary: Microwave and ohmic heating are popular choices for aseptic processing due to their rapid heating capabilities and better product quality. This study aims to find an alternative approach to simulate processing conditions by modeling and validating the process using surrogate particles and microorganisms. A mathematical model was used to simulate heat transfer in fluid and solid particles. The process was validated using simulated particles and surrogate microorganisms, with magnetic implants in spherical particles to measure residence time distribution. The microwave aseptic system was successfully validated for producing shelf-stable particulate food products.
FOOD AND BIOPRODUCTS PROCESSING
(2024)
