Frequency of off-targeting in genome edited pigs produced via direct injection of the CRISPR/Cas9 system into developing embryos

Infection dynamics of hepatitis E virus in wild-type and immunoglobulin heavy chain knockout JH (-/-) gnotobiotic piglets

(2018) Danielle M. Yugo et al.   JOURNAL OF VIROLOGY

Generation of a TP53-modified porcine cancer model by CRISPR/Cas9-mediated gene modification in porcine zygotes via electroporation

(2018) Fuminori Tanihara et al.   PLoS One

Genome Editing of Pigs for Agriculture and Biomedicine

(2018) Huaqiang Yang et al.   Frontiers in Genetics

Genome-edited baby claim provokes international outcry

(2018) David Cyranoski et al.   NATURE

Trio-Based Deep Sequencing Reveals a Low Incidence of Off-Target Mutations in the Offspring of Genetically Edited Goats

(2018) Chao Li et al.   Frontiers in Genetics

Correction of a pathogenic gene mutation in human embryos

(2017) Hong Ma et al.   NATURE

Profiling single-guide RNA specificity reveals a mismatch sensitive core sequence

(2017) Ting Zheng et al.   Scientific Reports

Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9

(2016) John G Doench et al.   NATURE BIOTECHNOLOGY

Generation ofRUNX3knockout pigs using CRISPR/Cas9-mediated gene targeting

(2016) J-T Kang et al.   REPRODUCTION IN DOMESTIC ANIMALS

Zygote injection of CRISPR/Cas9 RNA successfully modifies the target gene without delaying blastocyst development or altering the sex ratio in pigs

(2016) Kristin M. Whitworth et al.   TRANSGENIC RESEARCH

Efficient production of biallelicGGTA1knockout pigsbycytoplasmic microinjection of CRISPR/Cas9 into zygotes

(2016) Bjoern Petersen et al.   XENOTRANSPLANTATION

Partial loss of interleukin 2 receptor gamma function in pigs provides mechanistic insights for the study of human immunodeficiency syndrome

(2016) Yun-Jung Choi et al.   Oncotarget

Increased and prolonged human norovirus infection in RAG2/IL2RG deficient gnotobiotic pigs with severe combined immunodeficiency

(2016) Shaohua Lei et al.   Scientific Reports

Comparison of T7E1 and Surveyor Mismatch Cleavage Assays to Detect Mutations Triggered by Engineered Nucleases

(2015) Léna Vouillot et al.   G3-Genes Genomes Genetics

Off-target Effects in CRISPR/Cas9-mediated Genome Engineering

(2015) Xiao-Hui Zhang et al.   Molecular Therapy-Nucleic Acids

Use of the CRISPR/Cas9 System to Produce Genetically Engineered Pigs from In Vitro-Derived Oocytes and Embryos1

(2014) Kristin M. Whitworth et al.   BIOLOGY OF REPRODUCTION

Engraftment of human iPS cells and allogeneic porcine cells into pigs with inactivated RAG2 and accompanying severe combined immunodeficiency

(2014) K. Lee et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells

(2013) Yanfang Fu et al.   NATURE BIOTECHNOLOGY

Multiplex Genome Engineering Using CRISPR/Cas Systems

(2013) L. Cong et al.   SCIENCE

ZFN, TALEN, and CRISPR/Cas-based methods for genome engineering

(2013) Thomas Gaj et al.   TRENDS IN BIOTECHNOLOGY

Efficient TALEN-mediated gene knockout in livestock

(2012) D. F. Carlson et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Generation of PPARγ mono-allelic knockout pigs via zinc-finger nucleases and nuclear transfer cloning

(2011) Dongshan Yang et al.   CELL RESEARCH

Accurate and comprehensive sequencing of personal genomes

(2011) S. S. Ajay et al.   GENOME RESEARCH

Pig genome sequence - analysis and publication strategy

(2010) Alan L Archibald et al.   BMC GENOMICS

From Zygote to Implantation: Morphological and Molecular Dynamics during Embryo Development in the Pig

(2009) O Oestrup et al.   REPRODUCTION IN DOMESTIC ANIMALS