Article
Chemistry, Medicinal
Yuhao Jv, Chenxiang Xi, Yanqiu Zhao, Wei Wang, Yiling Zhang, Kai Liu, Wenlin Liu, Kai Shan, Chunlei Wang, Ruiwen Cao, Cunxi Dai, Yiting Jv, Wenxing Zhu, Haiyong Wang, Qiuxia He, Lujiang Hao
Summary: The genomic and carbohydrate metabolic features of P. agarivorans Hao 2018 were investigated through pan-genomic and transcriptomic analyses. These analyses revealed strain-specific metabolisms and the ability to utilize various carbohydrates. Furthermore, the study identified key genes involved in extracellular polysaccharide synthesis and their expression patterns in different growth phases. These findings provide potential for the development of novel marine drug lead compounds with high biological activity.
Article
Chemistry, Medicinal
Yuhao Ju, Kai Shan, Wenlin Liu, Chenxiang Xi, Yiling Zhang, Wei Wang, Chunlei Wang, Ruiwen Cao, Wenxing Zhu, Haiyong Wang, Yanqiu Zhao, Lujiang Hao
Summary: This study investigates the effects of different initial fermentation pH levels on the production and composition of exopolysaccharides (EPSs) in Pseudoalteromonas agarivorans Hao 2018. The transcriptome analysis reveals that genes involved in EPS synthesis are regulated by changes in environmental pH. These findings contribute to understanding the environmental adaptation mechanisms of P. agarivorans and provide guidance for the development of new products using biologically active polysaccharides.
Article
Chemistry, Medicinal
Xiyan Wang, Thomas Isbrandt, Mikael Lenz Strube, Sara Skott Paulsen, Maike Wennekers Nielsen, Yannick Buijs, Erwin M. Schoof, Thomas Ostenfeld Larsen, Lone Gram, Sheng-Da Zhang
Summary: Genome mining of pigmented Pseudoalteromonas has shown potential for bioactive compound and enzyme production. Proteomic analysis of Pseudoalteromonas rubra strain S4059 revealed an efficient chitin degradation system when grown on chitin. Deletion of GH19 chitinase in S4059 did not affect chitin degrading ability or secondary metabolite profile, suggesting its industrial potential.
Article
Chemistry, Medicinal
Xun-Ke Sun, Ya Gong, Dan-Dan Shang, Bang-Tao Liu, Zong-Jun Du, Guan-Jun Chen
Summary: Alginate is a major component of brown algae, crucial in marine ecosystems. In this study, 100 alginate-degrading strains were isolated from adult abalones, with Agarivorans sp. B2Z047 exhibiting the strongest degradation ability. Whole genome sequencing identified 12 potential alginate lyase genes, revealing the role of these lyases in the alginate degradation process.
Article
Marine & Freshwater Biology
Kaiyang Zheng, Yue Dong, Yantao Liang, Yundan Liu, Xinran Zhang, Wenjing Zhang, Ziyue Wang, Hongbing Shao, Yeong Yik Sung, Wen Jye Mok, Li Lian Wong, Andrew McMinn, Min Wang
Summary: This study systematically analyzed the genomes, taxonomic features, and distribution patterns of Pseudoalteromonas phages. A total of 143 Pseudoalteromonas-associated phage genomes were identified and assigned to 47 viral clusters. The prevalence of integrated proviruses and filamentous phages suggest the viral lysogenic life cycle in Pseudoalteromonas. These phages play a role in marine biogeochemical cycles and their abundance and distribution vary across polar areas and the deep sea. This study expands our understanding of marine Pseudoalteromonas phages and their interactions with their hosts.
MARINE LIFE SCIENCE & TECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Nor Hawani Salikin, Malak Dubois, Jadranka Nappi, Helene Lebhar, Christopher Marquis, Suhelen Egan
Summary: A new protein Nkp-1 produced by marine epiphytic bacterium was found to have nematocidal effects, causing nematode death within 24 hours of exposure. Additionally, nematodes may use defense strategies such as deformed anal region and internal hatching to counteract the toxicity of Nkp-1.
Article
Food Science & Technology
Wenyan Xie, Yuxian You, Xiaofeng Ban, Anqi Zhang, Caiming Li, Zhengbiao Gu, Zhaofeng Li
Summary: In this study, a cold-activated agarase with outstanding cold adaptation was cloned and expressed. The enzyme exhibited enhanced activity after low-temperature incubation and retained high activity at low temperatures. Structural changes were observed in the enzyme after low-temperature treatment. The carbohydrate-binding modules and linker region were found to play a crucial role in cold adaptation.
Article
Microbiology
Aide Lasa, Manon Auguste, Alberto Lema, Caterina Oliveri, Alessio Borello, Elisa Taviani, Guido Bonello, Lapo Doni, Andrew D. Millard, Maxime Bruto, Jesus L. Romalde, Michail Yakimov, Teresa Balbi, Carla Pruzzo, Laura Canesi, Luigi Vezzulli
Summary: Evolution of virulence traits in marine microbial pathogens may arise from adaptation to environmental niches beyond the host, which could be crucial for understanding their emergence and pathogenic potential. A newly discovered marine bacterial species, Vibrio bathopelagicus sp. nov., isolated from the deep Mediterranean Sea waters, exhibits genotypic and phenotypic traits associated with animal pathogenicity. This planktonic marine bacterium may have played an evolutionary role in the origin of coastal marine pathogens.
ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Zhipeng Li, Zeping Du, Hebin Li, Yanhong Chen, Mingjing Zheng, Zedong Jiang, Xiping Du, Hui Ni, Yanbing Zhu
Summary: Marine bacterium Microbulbifer sp. ALW1 can effectively degrade Laminaria japonica thallus fragments and produce various enzymes. ALW1 has unique cell morphology, physiological, and biochemical characteristics compared to other related species. The complete genome of ALW1 has been sequenced and can be utilized in potential industrial applications.
Article
Microbiology
Christopher Riccardi, Marzia Calvanese, Veronica Ghini, Tania Alonso-Vasquez, Elena Perrin, Paola Turano, Giorgio Giurato, Alessandro Weisz, Ermenegilda Parrilli, Maria Luisa Tutino, Marco Fondi
Summary: This study investigates the maintenance of metabolic homeostasis in a cold-adapted marine bacterium during growth at significantly different temperatures. The research shows a strong metabolic stability at the level of the main central metabolites but significant changes in gene expression at the transcriptional level. These findings suggest a transcriptomic buffering of cellular metabolism to produce overlapping metabolic phenotypes despite the temperature gap.
Article
Chemistry, Medicinal
Congling Liu, Gong Chen, Hailian Rao, Xun Xiao, Yidan Chen, Cuiling Wu, Fei Bian, Hailun He
Summary: Marine bacterium Pseudoalteromonas sp. JS4-1 was used to produce crude enzymes for hydrolyzing phycobiliprotein. The enzymatic productions showed good performance in scavenging DPPH radical and hydroxyl radical, especially small peptides with MWCO <3 kDa. The second fraction (F2) obtained from size-exclusion chromatography had the highest hydroxyl radical scavenging ability (62.61 +/- 5.80%). Both F1 and F2 exhibited good antioxidant activities in oxidative stress models in HUVECs and HaCaT cells.
Article
Microbiology
Alka Kumari, Nasreen Bano, Sumit Kumar Bag, Doongar R. Chaudhary, Bhavanath Jha
Summary: The study confirms that marine Bacillus species AIIW2 can grow on PET surfaces and cause structural deterioration, revealing relevant genes involved in PET utilization through transcriptomic analysis. In the presence of PET in the culture medium, strain AIIW2 enhances the expression of genes related to carbon metabolism and cell transport systems, while sporulation genes are highly expressed in the absence of PET.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Multidisciplinary Sciences
Wiebke Mohr, Nadine Lehnen, Soeren Ahmerkamp, Hannah K. Marchant, Jon S. Graf, Bernhard Tschitschko, Pelin Yilmaz, Sten Littmann, Harald Gruber-Vodicka, Nikolaus Leisch, Miriam Weber, Christian Lott, Carsten J. Schubert, Jana Milucka, Marcel M. M. Kuypers
Summary: Symbiotic N-2-fixing microorganisms play a crucial role in nitrogen assimilation by eukaryotes in nitrogen-limited environments. The symbiotic relationship between 'Candidatus Celerinatantimonas neptuna' and its host Posidonia oceanica allows highly productive seagrass meadows to thrive in the Mediterranean Sea. This symbiosis is similar to that of terrestrial N-2-fixing plant symbioses and may have enabled flowering plants to thrive in nitrogen-poor marine habitats.
Article
Microbiology
Olga I. Nedashkovkaya, Song-Gun Kim, Larissa A. Balabanova, Natalia V. Zhukova, Oksana M. Son, Liudmila A. Tekutyeva, Valery V. Mikhailov
Summary: A bacterium strain 16-SW-7 was isolated from a seawater sample, which could produce a unique enzyme converting B red blood cells into the universal type blood cells. Phylogenetic analysis revealed that strain 16-SW-7 is a member of the Pseudoalteromonas genus. The strain's genome consists of two circular chromosomes and shows high similarity to the closely related strains KMM 638(T) and KMM 3548(T).
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Endocrinology & Metabolism
Nathan Carriot, Raphaelle Barry-Martinet, Jean-Francois Briand, Annick Ortalo-Magne, Gerald Culioli
Summary: This study analyzed the effects of a gradient of phosphate concentrations on the biofilm-forming capacity and metabolome of the marine bacterium Pseudoalteromonas lipolytica TC8. The results showed that lower phosphate concentrations led to decreased biomass, carbohydrate content, and three-dimensional structures of biofilms. Untargeted metabolomics revealed differences in metabolites at different phosphate concentrations, with ornithine lipids increasing and oxidized phosphatidylethanolamines decreasing as phosphate concentration decreased.
Article
Biochemistry & Molecular Biology
Baolei Jia, De Peng Yuan, Wen Jun Lan, Yuan Hu Xuan, Che Ok Jeon
Article
Biology
Bing Li, Xiaotao Jia, Yuxia Chi, Xinli Liu, Baolei Jia
JOURNAL OF BIOLOGICAL EDUCATION
(2020)
Article
Microbiology
Baolei Jia, Che Ok Jeon
Article
Biotechnology & Applied Microbiology
Lingtong Liao, Yuling Meng, Ruiming Wang, Baolei Jia, Piwu Li
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2019)
Article
Gastroenterology & Hepatology
Baolei Jia, Dongbin Park, Yoonsoo Hahn, Che Ok Jeon
Article
Biochemistry & Molecular Biology
Baolei Jia, Dongbin Park, Byung Hee Chun, Yoonsoo Hahn, Che Ok Jeon
Summary: The study indicates that different dietary patterns can affect the abundance of bile salt hydrolases (BSHs) and the community structure of BSH-active bacteria, with a ketogenic diet (KD) increasing BSH abundance and altering the community structure of BSH-active bacteria.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Xue Li, Xinfeng Tan, Qingshuang Chen, Xiaoling Zhu, Jing Zhang, Jie Zhang, Baolei Jia
Summary: Oral administration of prodigiosin can improve the intestinal microbiota of mice, demonstrating potential probiotic effects.
Article
Biochemistry & Molecular Biology
Yuling Meng, Fei Zhao, Xianwei Jin, Yun Feng, Gangzheng Sun, Junzhang Lin, Baolei Jia, Piwu Li
Summary: This study demonstrated that mannanase II is an effective enzyme breaker for fracturing operations in alkaline and medium-temperature reservoirs. Mannanase II showed excellent gel-breaking performance, while requiring only one fifth of the concentration needed for other enzyme breakers. Additionally, it had minimal adverse effects on the fracturing fluid's performance and generated significantly lower residue compared to other breaker additives.
Review
Biotechnology & Applied Microbiology
Baolei Jia, Xiao Han, Kyung Hyun Kim, Che Ok Jeon
Summary: This article discusses the recent efforts and technologies used for discovering and mining enzymes from the human gut microbiota. The wealth of knowledge on metabolites, reactions, genome sequences, and protein structures may lead to the development of strategies for enzyme mining. Ongoing efforts to annotate gut microbiota enzymes may elucidate catalytic mechanisms that could guide the clinical applications of the gut microbiome for diagnostic and therapeutic purposes.
TRENDS IN BIOTECHNOLOGY
(2022)
Article
Green & Sustainable Science & Technology
Pengfei Xu, Xiaoling Zhu, Huashang Tian, Guangxu Zhao, Yuxia Chi, Baolei Jia, Jie Zhang
Summary: In this study, bibliometric analysis was used to assess the applications of humic acids (HAs) in the environmental field. The results showed that HAs play important roles in environmental remediation, particularly in the treatment of organic pollutants and heavy metals. However, the molecular structure of HAs remains unclear, limiting their further applications.
JOURNAL OF CLEANER PRODUCTION
(2022)
Letter
Public, Environmental & Occupational Health
Chenqinyao Li, Xiaoling Zhu, Yun Zhang, Jie Zhang, Che Ok Jeon, Baolei Jia
TRAVEL MEDICINE AND INFECTIOUS DISEASE
(2022)
Letter
Infectious Diseases
Chenqinyao Li, Ju Hye Baek, Jiazhang Huang, Baolei Jia
NEW MICROBES AND NEW INFECTIONS
(2023)
Article
Environmental Sciences
Pengfei Xu, Shenghui Dong, Xinyuan Luo, Bin Wei, Cong Zhang, Xinyao Ji, Jing Zhang, Xiaoling Zhu, Guangfan Meng, Baolei Jia, Jie Zhang
Summary: In this study, it was found that naturally occurring humic acids can alleviate AFB1-induced liver cell swelling and inflammation, and reduce oxidative stress and inflammatory responses. Moreover, humic acids can restore intestinal permeability, regulate gut microbiota, and absorb toxins. Therefore, treatment with humic acids can ameliorate AFB1-induced hepatic injury.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2023)
Review
Biotechnology & Applied Microbiology
Aiqin Shi, Yan Liu, Baolei Jia, Gang Zheng, Yanlai Yao
Summary: Metabolic engineering is a promising strategy for environmentally friendly synthesis of valuable petroleum-derived chemicals. This review focuses on the production of L-aspartate and its derivatives, such as L-β-alanine, ectoine, 3-hydroxypropionate, D-pantothenic acid, and L-homoserine, in cell factories. The functions, applications, and current research progress on microbial production of these compounds are discussed, along with suggestions to improve the industrial application of engineered bacteria.
FERMENTATION-BASEL
(2023)
