The importance of extracellular vesicle purification for downstream analysis: A comparison of differential centrifugation and size exclusion chromatography for helminth pathogens

Higher functionality of extracellular vesicles isolated using size-exclusion chromatography compared to ultracentrifugation

(2017) Emma A. Mol et al.   Nanomedicine-Nanotechnology Biology and Medicine

On the presence and immunoregulatory functions of extracellular microRNAs in the trematodeFasciola hepatica

(2017) B. Fromm et al.   PARASITE IMMUNOLOGY

Multiple extracellular vesicle types in peritoneal dialysis effluent are prominent and contain known biomarkers

(2017) Lachlan James Pearson et al.   PLoS One

Exosome and Microvesicle-Enriched Fractions Isolated from Mesenchymal Stem Cells by Gradient Separation Showed Different Molecular Signatures and Functions on Renal Tubular Epithelial Cells

(2017) Federica Collino et al.   Stem Cell Reviews and Reports

Malaria parasite DNA-harbouring vesicles activate cytosolic immune sensors

(2017) Xavier Sisquella et al.   Nature Communications

Ultrafiltration combined with size exclusion chromatography efficiently isolates extracellular vesicles from cell culture media for compositional and functional studies

(2017) Birke J. Benedikter et al.   Scientific Reports

Extracellular Vesicles from a Helminth Parasite Suppress Macrophage Activation and Constitute an Effective Vaccine for Protective Immunity

(2017) Gillian Coakley et al.   Cell Reports

Exosome and Microvesicle-Enriched Fractions Isolated from Mesenchymal Stem Cells by Gradient Separation Showed Different Molecular Signatures and Functions on Renal Tubular Epithelial Cells

(2017) Federica Collino et al.   Stem Cell Reviews and Reports

Exosomes Derived from Dendritic Cells Treated with Schistosoma japonicum Soluble Egg Antigen Attenuate DSS-Induced Colitis

(2017) Lifu Wang et al.   Frontiers in Pharmacology

MicroRNA Signatures and Molecular Subtypes of Glioblastoma: The Role of Extracellular Transfer

(2017) Jakub Godlewski et al.   Stem Cell Reports

Extracellular Vesicles from Trypanosoma brucei Mediate Virulence Factor Transfer and Cause Host Anemia

(2016) Anthony J. Szempruch et al.   CELL

Extracellular vesicles secreted by Schistosoma mansoni contain protein vaccine candidates

(2016) Javier Sotillo et al.   INTERNATIONAL JOURNAL FOR PARASITOLOGY

Chromatography and its hyphenation to mass spectrometry for extracellular vesicle analysis

(2016) Gabriella Pocsfalvi et al.   JOURNAL OF CHROMATOGRAPHY A

Extracellular vesicles and infectious diseases: new complexity to an old story

(2016) Jeffrey S. Schorey et al.   JOURNAL OF CLINICAL INVESTIGATION

Gene Expression Profiling and Functional Characterization of Macrophages in Response to Circulatory Microparticles Produced during Trypanosoma cruzi Infection and Chagas Disease

(2016) Imran H. Chowdhury et al.   Journal of Innate Immunity

Exploring and Expanding the Fatty-Acid-Binding Protein Superfamily in Fasciola Species

(2016) Russell M. Morphew et al.   JOURNAL OF PROTEOME RESEARCH

Exosomes Secreted by Toxoplasma gondii-Infected L6 Cells: Their Effects on Host Cell Proliferation and Cell Cycle Changes

(2016) Min Jae Kim et al.   KOREAN JOURNAL OF PARASITOLOGY

Proteomic analysis of exosomal cargo: the challenge of high purity vesicle isolation

(2016) Agata Abramowicz et al.   Molecular BioSystems

Proteomic comparison defines novel markers to characterize heterogeneous populations of extracellular vesicle subtypes

(2016) Joanna Kowal et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A preliminary proteomic characterisation of extracellular vesicles released by the ovine parasitic nematode, Teladorsagia circumcincta

(2016) Thomas Tzelos et al.   VETERINARY PARASITOLOGY

Infected erythrocyte-derived extracellular vesicles alter vascular function via regulatory Ago2-miRNA complexes in malaria

(2016) Pierre-Yves Mantel et al.   Nature Communications

Cells release subpopulations of exosomes with distinct molecular and biological properties

(2016) Eduard Willms et al.   Scientific Reports

Across intra-mammalian stages of the liver f luke Fasciola hepatica: a proteomic study

(2016) Lucía Sánchez Di Maggio et al.   Scientific Reports

Size-Exclusion Chromatography-based isolation minimally alters Extracellular Vesicles’ characteristics compared to precipitating agents

(2016) Ana Gámez-Valero et al.   Scientific Reports

Exosomes: improved methods to characterize their morphology, RNA content, and surface protein biomarkers

(2015) Yueting Wu et al.   ANALYST

Isolation of extracellular vesicles: Determining the correct approach (Review)

(2015) RAFAL SZATANEK et al.   INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE

Carcinogenic Liver Fluke Secretes Extracellular Vesicles That Promote Cholangiocytes to Adopt a Tumorigenic Phenotype

(2015) Sujittra Chaiyadet et al.   JOURNAL OF INFECTIOUS DISEASES

miRNA contents of cerebrospinal fluid extracellular vesicles in glioblastoma patients

(2015) Johnny C. Akers et al.   JOURNAL OF NEURO-ONCOLOGY

Secretion of RNA-Containing Extracellular Vesicles by the Porcine Whipworm,Trichuris suis

(2015) Eline Palm Hansen et al.   JOURNAL OF PARASITOLOGY

The Extracellular Vesicles of the Helminth Pathogen,Fasciola hepatica: Biogenesis Pathways and Cargo Molecules Involved in Parasite Pathogenesis

(2015) Krystyna Cwiklinski et al.   MOLECULAR & CELLULAR PROTEOMICS

Ultrafiltration with size-exclusion liquid chromatography for high yield isolation of extracellular vesicles preserving intact biophysical and functional properties

(2015) Joel Z. Nordin et al.   Nanomedicine-Nanotechnology Biology and Medicine

Exosome-like vesicles derived by Schistosoma japonicum adult worms mediates M1 type immune- activity of macrophage

(2015) Lifu Wang et al.   PARASITOLOGY RESEARCH

A new enabling proteomics methodology to investigate membrane associated proteins from parasitic nematodes: Case study using ivermectin resistant and ivermectin susceptible isolates of Caenorhabditis elegans and Haemonchus contortus

(2015) Elizabeth H. Hart et al.   VETERINARY PARASITOLOGY

Heparin affinity purification of extracellular vesicles

(2015) Leonora Balaj et al.   Scientific Reports

Isolation of exosomes by differential centrifugation: Theoretical analysis of a commonly used protocol

(2015) Mikhail A. Livshits et al.   Scientific Reports

Release of Small RNA-containing Exosome-like Vesicles from the Human Filarial Parasite Brugia malayi

(2015) Mostafa Zamanian et al.   PLoS Neglected Tropical Diseases

Isolation of High-Purity Extracellular Vesicles by Extracting Proteins Using Aqueous Two-Phase System

(2015) Jongmin Kim et al.   PLoS One

Isolation of Exosomes from Blood Plasma: Qualitative and Quantitative Comparison of Ultracentrifugation and Size Exclusion Chromatography Methods

(2015) Tamás Baranyai et al.   PLoS One

Exosomes and other extracellular vesicles in host-pathogen interactions

(2014) J. S. Schorey et al.   EMBO REPORTS

Different gDNA content in the subpopulations of prostate cancer extracellular vesicles: Apoptotic bodies, microvesicles, and exosomes

(2014) Elisa Lázaro-Ibáñez et al.   PROSTATE

Generating a detailed protein profile ofFasciola hepaticaduring the chronic stage of infection in cattle

(2014) Orçun Haçarız et al.   PROTEOMICS

Update on Controls for Isolation and Quantification Methodology of Extracellular Vesicles Derived from Adipose Tissue Mesenchymal Stem Cells

(2014) Marcella Franquesa et al.   Frontiers in Immunology

Cell-Cell Communication between Malaria-Infected Red Blood Cells via Exosome-like Vesicles

(2013) Neta Regev-Rudzki et al.   CELL

Malaria-Infected Erythrocyte-Derived Microvesicles Mediate Cellular Communication within the Parasite Population and with the Host Immune System

(2013) Pierre-Yves Mantel et al.   Cell Host & Microbe

Transcriptome of Extracellular Vesicles Released by Hepatocytes

(2013) Felix Royo et al.   PLoS One

Trichomonas vaginalis Exosomes Deliver Cargo to Host Cells and Mediate Host∶Parasite Interactions

(2013) Olivia Twu et al.   PLoS Pathogens

Analysis of Fasciola cathepsin L5 by S2 subsite substitutions and determination of the P1–P4 specificity reveals an unusual preference

(2012) Luke J. Norbury et al.   BIOCHIMIE

Extracellular Vesicles from Parasitic Helminths Contain Specific Excretory/Secretory Proteins and Are Internalized in Intestinal Host Cells

(2012) Antonio Marcilla et al.   PLoS One

The Sigma Class Glutathione Transferase from the Liver Fluke Fasciola hepatica

(2012) E. James LaCourse et al.   PLoS Neglected Tropical Diseases

Exploring the Fasciola hepatica tegument proteome

(2011) R. Alan Wilson et al.   INTERNATIONAL JOURNAL FOR PARASITOLOGY

Towards Delineating Functions within the Fasciola Secreted Cathepsin L Protease Family by Integrating In Vivo Based Sub-Proteomics and Phylogenetics

(2011) Russell M. Morphew et al.   PLoS Neglected Tropical Diseases

Major Secretory Antigens of the Helminth Fasciola hepatica Activate a Suppressive Dendritic Cell Phenotype That Attenuates Th17 Cells but Fails To Activate Th2 Immune Responses

(2009) D. J. Dowling et al.   INFECTION AND IMMUNITY

An Integrated Transcriptomics and Proteomics Analysis of the Secretome of the Helminth PathogenFasciola hepatica

(2009) Mark W. Robinson et al.   MOLECULAR & CELLULAR PROTEOMICS