The importance of extracellular vesicle purification for downstream analysis: A comparison of differential centrifugation and size exclusion chromatography for helminth pathogens
出版年份 2019 全文链接
标题
The importance of extracellular vesicle purification for downstream analysis: A comparison of differential centrifugation and size exclusion chromatography for helminth pathogens
作者
关键词
Tegument proteins, Vesicles, Membrane proteins, Size-exclusion chromatography, Differential centrifugation, Gene ontologies, Helminths, Culture media
出版物
PLoS Neglected Tropical Diseases
Volume 13, Issue 2, Pages e0007191
出版商
Public Library of Science (PLoS)
发表日期
2019-02-28
DOI
10.1371/journal.pntd.0007191
参考文献
相关参考文献
注意：仅列出部分参考文献，下载原文获取全部文献信息。- Higher functionality of extracellular vesicles isolated using size-exclusion chromatography compared to ultracentrifugation
- (2017) Emma A. Mol et al. Nanomedicine-Nanotechnology Biology and Medicine
- On the presence and immunoregulatory functions of extracellular microRNAs in the trematodeFasciola hepatica
- (2017) B. Fromm et al. PARASITE IMMUNOLOGY
- Multiple extracellular vesicle types in peritoneal dialysis effluent are prominent and contain known biomarkers
- (2017) Lachlan James Pearson et al. PLoS One
- Exosome and Microvesicle-Enriched Fractions Isolated from Mesenchymal Stem Cells by Gradient Separation Showed Different Molecular Signatures and Functions on Renal Tubular Epithelial Cells
- (2017) Federica Collino et al. Stem Cell Reviews and Reports
- Malaria parasite DNA-harbouring vesicles activate cytosolic immune sensors
- (2017) Xavier Sisquella et al. Nature Communications
- Ultrafiltration combined with size exclusion chromatography efficiently isolates extracellular vesicles from cell culture media for compositional and functional studies
- (2017) Birke J. Benedikter et al. Scientific Reports
- Extracellular Vesicles from a Helminth Parasite Suppress Macrophage Activation and Constitute an Effective Vaccine for Protective Immunity
- (2017) Gillian Coakley et al. Cell Reports
- Exosome and Microvesicle-Enriched Fractions Isolated from Mesenchymal Stem Cells by Gradient Separation Showed Different Molecular Signatures and Functions on Renal Tubular Epithelial Cells
- (2017) Federica Collino et al. Stem Cell Reviews and Reports
- Exosomes Derived from Dendritic Cells Treated with Schistosoma japonicum Soluble Egg Antigen Attenuate DSS-Induced Colitis
- (2017) Lifu Wang et al. Frontiers in Pharmacology
- MicroRNA Signatures and Molecular Subtypes of Glioblastoma: The Role of Extracellular Transfer
- (2017) Jakub Godlewski et al. Stem Cell Reports
- Extracellular Vesicles from Trypanosoma brucei Mediate Virulence Factor Transfer and Cause Host Anemia
- (2016) Anthony J. Szempruch et al. CELL
- Extracellular vesicles secreted by Schistosoma mansoni contain protein vaccine candidates
- (2016) Javier Sotillo et al. INTERNATIONAL JOURNAL FOR PARASITOLOGY
- Chromatography and its hyphenation to mass spectrometry for extracellular vesicle analysis
- (2016) Gabriella Pocsfalvi et al. JOURNAL OF CHROMATOGRAPHY A
- Extracellular vesicles and infectious diseases: new complexity to an old story
- (2016) Jeffrey S. Schorey et al. JOURNAL OF CLINICAL INVESTIGATION
- Gene Expression Profiling and Functional Characterization of Macrophages in Response to Circulatory Microparticles Produced during Trypanosoma cruzi Infection and Chagas Disease
- (2016) Imran H. Chowdhury et al. Journal of Innate Immunity
- Exploring and Expanding the Fatty-Acid-Binding Protein Superfamily in Fasciola Species
- (2016) Russell M. Morphew et al. JOURNAL OF PROTEOME RESEARCH
- Exosomes Secreted by Toxoplasma gondii-Infected L6 Cells: Their Effects on Host Cell Proliferation and Cell Cycle Changes
- (2016) Min Jae Kim et al. KOREAN JOURNAL OF PARASITOLOGY
- Proteomic analysis of exosomal cargo: the challenge of high purity vesicle isolation
- (2016) Agata Abramowicz et al. Molecular BioSystems
- Proteomic comparison defines novel markers to characterize heterogeneous populations of extracellular vesicle subtypes
- (2016) Joanna Kowal et al. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
- A preliminary proteomic characterisation of extracellular vesicles released by the ovine parasitic nematode, Teladorsagia circumcincta
- (2016) Thomas Tzelos et al. VETERINARY PARASITOLOGY
- Infected erythrocyte-derived extracellular vesicles alter vascular function via regulatory Ago2-miRNA complexes in malaria
- (2016) Pierre-Yves Mantel et al. Nature Communications
- Cells release subpopulations of exosomes with distinct molecular and biological properties
- (2016) Eduard Willms et al. Scientific Reports
- Across intra-mammalian stages of the liver f luke Fasciola hepatica: a proteomic study
- (2016) Lucía Sánchez Di Maggio et al. Scientific Reports
- Size-Exclusion Chromatography-based isolation minimally alters Extracellular Vesicles’ characteristics compared to precipitating agents
- (2016) Ana Gámez-Valero et al. Scientific Reports
- Exosomes: improved methods to characterize their morphology, RNA content, and surface protein biomarkers
- (2015) Yueting Wu et al. ANALYST
- Isolation of extracellular vesicles: Determining the correct approach (Review)
- (2015) RAFAL SZATANEK et al. INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
- Carcinogenic Liver Fluke Secretes Extracellular Vesicles That Promote Cholangiocytes to Adopt a Tumorigenic Phenotype
- (2015) Sujittra Chaiyadet et al. JOURNAL OF INFECTIOUS DISEASES
- miRNA contents of cerebrospinal fluid extracellular vesicles in glioblastoma patients
- (2015) Johnny C. Akers et al. JOURNAL OF NEURO-ONCOLOGY
- Secretion of RNA-Containing Extracellular Vesicles by the Porcine Whipworm,Trichuris suis
- (2015) Eline Palm Hansen et al. JOURNAL OF PARASITOLOGY
- The Extracellular Vesicles of the Helminth Pathogen,Fasciola hepatica: Biogenesis Pathways and Cargo Molecules Involved in Parasite Pathogenesis
- (2015) Krystyna Cwiklinski et al. MOLECULAR & CELLULAR PROTEOMICS
- Ultrafiltration with size-exclusion liquid chromatography for high yield isolation of extracellular vesicles preserving intact biophysical and functional properties
- (2015) Joel Z. Nordin et al. Nanomedicine-Nanotechnology Biology and Medicine
- Exosome-like vesicles derived by Schistosoma japonicum adult worms mediates M1 type immune- activity of macrophage
- (2015) Lifu Wang et al. PARASITOLOGY RESEARCH
- A new enabling proteomics methodology to investigate membrane associated proteins from parasitic nematodes: Case study using ivermectin resistant and ivermectin susceptible isolates of Caenorhabditis elegans and Haemonchus contortus
- (2015) Elizabeth H. Hart et al. VETERINARY PARASITOLOGY
- Heparin affinity purification of extracellular vesicles
- (2015) Leonora Balaj et al. Scientific Reports
- Isolation of exosomes by differential centrifugation: Theoretical analysis of a commonly used protocol
- (2015) Mikhail A. Livshits et al. Scientific Reports
- Release of Small RNA-containing Exosome-like Vesicles from the Human Filarial Parasite Brugia malayi
- (2015) Mostafa Zamanian et al. PLoS Neglected Tropical Diseases
- Isolation of High-Purity Extracellular Vesicles by Extracting Proteins Using Aqueous Two-Phase System
- (2015) Jongmin Kim et al. PLoS One
- Isolation of Exosomes from Blood Plasma: Qualitative and Quantitative Comparison of Ultracentrifugation and Size Exclusion Chromatography Methods
- (2015) Tamás Baranyai et al. PLoS One
- Exosomes and other extracellular vesicles in host-pathogen interactions
- (2014) J. S. Schorey et al. EMBO REPORTS
- Different gDNA content in the subpopulations of prostate cancer extracellular vesicles: Apoptotic bodies, microvesicles, and exosomes
- (2014) Elisa Lázaro-Ibáñez et al. PROSTATE
- Generating a detailed protein profile ofFasciola hepaticaduring the chronic stage of infection in cattle
- (2014) Orçun Haçarız et al. PROTEOMICS
- Update on Controls for Isolation and Quantification Methodology of Extracellular Vesicles Derived from Adipose Tissue Mesenchymal Stem Cells
- (2014) Marcella Franquesa et al. Frontiers in Immunology
- Cell-Cell Communication between Malaria-Infected Red Blood Cells via Exosome-like Vesicles
- (2013) Neta Regev-Rudzki et al. CELL
- Malaria-Infected Erythrocyte-Derived Microvesicles Mediate Cellular Communication within the Parasite Population and with the Host Immune System
- (2013) Pierre-Yves Mantel et al. Cell Host & Microbe
- Transcriptome of Extracellular Vesicles Released by Hepatocytes
- (2013) Felix Royo et al. PLoS One
- Trichomonas vaginalis Exosomes Deliver Cargo to Host Cells and Mediate Host∶Parasite Interactions
- (2013) Olivia Twu et al. PLoS Pathogens
- Analysis of Fasciola cathepsin L5 by S2 subsite substitutions and determination of the P1–P4 specificity reveals an unusual preference
- (2012) Luke J. Norbury et al. BIOCHIMIE
- Extracellular Vesicles from Parasitic Helminths Contain Specific Excretory/Secretory Proteins and Are Internalized in Intestinal Host Cells
- (2012) Antonio Marcilla et al. PLoS One
- The Sigma Class Glutathione Transferase from the Liver Fluke Fasciola hepatica
- (2012) E. James LaCourse et al. PLoS Neglected Tropical Diseases
- Exploring the Fasciola hepatica tegument proteome
- (2011) R. Alan Wilson et al. INTERNATIONAL JOURNAL FOR PARASITOLOGY
- Towards Delineating Functions within the Fasciola Secreted Cathepsin L Protease Family by Integrating In Vivo Based Sub-Proteomics and Phylogenetics
- (2011) Russell M. Morphew et al. PLoS Neglected Tropical Diseases
- Major Secretory Antigens of the Helminth Fasciola hepatica Activate a Suppressive Dendritic Cell Phenotype That Attenuates Th17 Cells but Fails To Activate Th2 Immune Responses
- (2009) D. J. Dowling et al. INFECTION AND IMMUNITY
- An Integrated Transcriptomics and Proteomics Analysis of the Secretome of the Helminth PathogenFasciola hepatica
- (2009) Mark W. Robinson et al. MOLECULAR & CELLULAR PROTEOMICS
Discover Peeref hubs
Discuss science. Find collaborators. Network.
Join a conversationCreate your own webinar
Interested in hosting your own webinar? Check the schedule and propose your idea to the Peeref Content Team.
Create Now