Identification of Thermus aquaticus DNA polymerase variants with increased mismatch discrimination and reverse transcriptase activity from a smart enzyme mutant library

Combinatorial Library Based on Restriction Enzyme-mediated Modular Assembly

(2017) Cuiping Ma et al.   ACS Combinatorial Science

Variants of sequence family B Thermococcus kodakaraensis DNA polymerase with increased mismatch extension selectivity

(2017) Claudia Huber et al.   PLoS One

Evolution of thermophilic DNA polymerases for the recognition and amplification of C2ʹ-modified DNA

(2016) Tingjian Chen et al.   Nature Chemistry

Direct and site-specific quantification of RNA 2′-O-methylation by PCR with an engineered DNA polymerase

(2016) Joos Aschenbrenner et al.   NUCLEIC ACIDS RESEARCH

5-methylcytosine-sensitive variants ofThermococcus kodakaraensisDNA polymerase

(2016) Claudia Huber et al.   NUCLEIC ACIDS RESEARCH

Synthetic evolutionary origin of a proofreading reverse transcriptase

(2016) J. W. Ellefson et al.   SCIENCE

Direct Sensing of 5-Methylcytosine by Polymerase Chain Reaction

(2014) Joos Aschenbrenner et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Variants of a Thermus aquaticus DNA Polymerase with Increased Selectivity for Applications in Allele- and Methylation-Specific Amplification

(2014) Matthias Drum et al.   PLoS One

Compartmentalized self-replication under fast PCR cycling conditions yields Taq DNA polymerase mutants with increased DNA-binding affinity and blood resistance

(2014) Bahram Arezi et al.   Frontiers in Microbiology

Structure and Function of an RNA-Reading Thermostable DNA Polymerase

(2013) Nina Blatter et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Cooperation between Catalytic and DNA Binding Domains Enhances Thermostability and Supports DNA Synthesis at Higher Temperatures by Thermostable DNA Polymerases

(2012) Andrey R. Pavlov et al.   BIOCHEMISTRY

Synthetic Genetic Polymers Capable of Heredity and Evolution

(2012) V. B. Pinheiro et al.   SCIENCE

HLA DNA typing: past, present, and future

(2012) H. Erlich   TISSUE ANTIGENS

Reducing Codon Redundancy and Screening Effort of Combinatorial Protein Libraries Created by Saturation Mutagenesis

(2012) Sabrina Kille et al.   ACS Synthetic Biology

Mutations to create thermostable reverse transcriptase with bacterial family A DNA polymerase from Thermotoga petrophila K4

(2011) Sotaro Sano et al.   JOURNAL OF BIOSCIENCE AND BIOENGINEERING

Allele-Specific Real-Time PCR System for Detection of Subpopulations of Genotype 1a and 1b Hepatitis C NS5B Y448H Mutant Viruses in Clinical Samples

(2011) Andrew S. Bae et al.   JOURNAL OF CLINICAL MICROBIOLOGY

Optimized Allele-Specific Real-Time PCR Assays for the Detection of Common Mutations in KRAS and BRAF

(2011) Alois H. Lang et al.   JOURNAL OF MOLECULAR DIAGNOSTICS

Molecular breeding of polymerases for resistance to environmental inhibitors

(2011) Claudia Baar et al.   NUCLEIC ACIDS RESEARCH

Directed Evolution of DNA Polymerases for Next-Generation Sequencing

(2010) Aaron M. Leconte et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

One-step RNA pathogen detection with reverse transcriptase activity of a mutated thermostable Thermus aquaticus DNA polymerase

(2010) Ramon Kranaster et al.   Biotechnology Journal

Replication through an abasic DNA lesion: structural basis for adenine selectivity

(2010) Samra Obeid et al.   EMBO JOURNAL

Human DNA Polymerase β Mutations Allowing Efficient Abasic Site Bypass

(2010) Sonja Gieseking et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Improvement of  29 DNA polymerase amplification performance by fusion of DNA binding motifs

(2010) M. de Vega et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Evolving a Polymerase for Hydrophobic Base Analogues

(2009) David Loakes et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY