Comprehensive analysis of heterotrimeric G-protein complex diversity and their interactions with GPCRs in solution

A cleavable ligand column for the rapid isolation of large quantities of homogeneous and functional neurotensin receptor 1 variants from E. coli

(2015) Pascal Egloff et al.   PROTEIN EXPRESSION AND PURIFICATION

Structure of signaling-competent neurotensin receptor 1 obtained by directed evolution inEscherichia coli

(2014) Pascal Egloff et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Structural features of the G-protein/GPCR interactions

(2013) Irina S. Moreira   BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS

Recent developments in biased agonism

(2013) James W Wisler et al.   CURRENT OPINION IN CELL BIOLOGY

Stabilizing membrane proteins through protein engineering

(2013) Daniel J Scott et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Interactions of the α-subunits of heterotrimeric G-proteins with GPCRs, effectors and RGS proteins: A critical review and analysis of interacting surfaces, conformational shifts, structural diversity and electrostatic potentials

(2013) Fotis A. Baltoumas et al.   JOURNAL OF STRUCTURAL BIOLOGY

Structure-Function of the G Protein–Coupled Receptor Superfamily

(2012) Vsevolod Katritch et al.   Annual Review of Pharmacology and Toxicology

Maximizing Detergent Stability and Functional Expression of a GPCR by Exhaustive Recombination and Evolution

(2012) Karola M. Schlinkmann et al.   JOURNAL OF MOLECULAR BIOLOGY

Direct Molecular Evolution of Detergent-Stable G Protein-Coupled Receptors Using Polymer Encapsulated Cells

(2012) Daniel J. Scott et al.   JOURNAL OF MOLECULAR BIOLOGY

Critical features for biosynthesis, stability, and functionality of a G protein-coupled receptor uncovered by all-versus-all mutations

(2012) K. M. Schlinkmann et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Robots, pipelines, polyproteins: Enabling multiprotein expression in prokaryotic and eukaryotic cells

(2011) Lakshmi Sumitra Vijayachandran et al.   JOURNAL OF STRUCTURAL BIOLOGY

Enabling high-throughput ligation-independent cloning and protein expression for the family of ubiquitin specific proteases

(2011) Mark P.A. Luna-Vargas et al.   JOURNAL OF STRUCTURAL BIOLOGY

Crystal structure of the β2 adrenergic receptor–Gs protein complex

(2011) Søren G. F. Rasmussen et al.   NATURE

Interaction of a G protein with an activated receptor opens the interdomain interface in the alpha subunit

(2011) N. Van Eps et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Structural flexibility of the G s  -helical domain in the  2-adrenoceptor Gs complex

(2011) G. H. Westfield et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

MultiBac: expanding the research toolbox for multiprotein complexes

(2011) Christoph Bieniossek et al.   TRENDS IN BIOCHEMICAL SCIENCES

The role of conformational ensembles of seven transmembrane receptors in functional selectivity

(2010) Nagarajan Vaidehi et al.   CURRENT OPINION IN PHARMACOLOGY

The quest to understand heterotrimeric G protein signaling

(2010) John J G Tesmer   NATURE STRUCTURAL & MOLECULAR BIOLOGY

A plasmid-based multigene expression system for mammalian cells

(2010) Andrijana Kriz et al.   Nature Communications

Gβ3 forms distinct dimers with specific Gγ subunits and preferentially activates the β3 isoform of phospholipase C

(2009) Lydia S.W. Poon et al.   CELLULAR SIGNALLING

Role of Molecular Chaperones in G Protein β5/Regulator of G Protein Signaling Dimer Assembly and G Protein βγ Dimer Specificity

(2009) Alyson C. Howlett et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Structural Determinants Involved in the Formation and Activation of G Protein βγ Dimers

(2009) William E. McIntire   NEUROSIGNALS

Structural diversity of G protein-coupled receptors and significance for drug discovery

(2008) Malin C. Lagerström et al.   NATURE REVIEWS DRUG DISCOVERY

Directed evolution of a G protein-coupled receptor for expression, stability, and binding selectivity

(2008) C. A. Sarkar et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA