Review
Pharmacology & Pharmacy
Abdur Rauf, Anees Ahmed Khalil, Ahmed Olatunde, Muneeb Khan, Sirajudheen Anwar, Ahmed Alafnan, Kannan R. R. Rengasamy
Summary: Marine habitats are rich in diverse life forms that offer potential sources of novel bioactive compounds, including protease inhibitors with significant applications in pharmaceutical, nutraceutical, and cosmeceutical industries. Despite extensive research on protease inhibitors, many compounds have not advanced to clinical trials, highlighting the ongoing need for exploring new sources for their development.
PHARMACOLOGICAL RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Yasel Guerra, Vinicio Armijos-Jaramillo, Tirso Pons, Eduardo Tejera, Colin Berry
Summary: The Kunitz-Soybean Trypsin Inhibitor (Kunitz-STI) family is a large family of proteins with most of its members being protease inhibitors. This study analyzed the crystallographic structures of Kunitz-STI inhibitors to characterize the structural features and locations of serine protease-binding loops. The findings suggest a relationship between the conformation of these loops, the inhibition mechanism, and the plant source of the inhibitors. This research challenges the perception that all Kunitz-STI inhibitors are canonical and will aid in the identification and design of multifunctional proteins.
Article
Biochemistry & Molecular Biology
Alejandro A. Puchol Tarazona, Daniel Maresch, Annette Grill, Janet Bakalarz, Juan A. Torres Acosta, Alexandra Castilho, Herta Steinkellner, Lukas Mach
Summary: Nicotiana benthamiana is a versatile host for manufacturing protein therapeutics, but the fidelity of recombinant proteins is compromised by proteolysis. Two subtilisin-like serine proteases (NbSBT1 and NbSBT2) have been identified as the main causes of proteolysis, suggesting that downregulating their activities could optimize the production of biopharmaceuticals on this platform. Sequence data of NbSBT are available in the DDBJ/EMBL/GenBank databases.
Review
Endocrinology & Metabolism
Yang Li, Liang Guo
Summary: Murine Serpina3c, a member of the Serpins family, has been shown to play roles in insulin secretion, adipogenesis, and the regulation of metabolic disorders such as NAFLD, insulin resistance, and obesity. Furthermore, Serpina3c has been found to have potential implications in atherosclerosis and cardiac remodeling after myocardial infarction. Its functions are largely mediated by the inhibition of serine protease activity. This article aims to summarize recent studies on Serpina3c to provide a comprehensive understanding of its biological roles and underlying mechanisms.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Roberto Arrigoni, Luigi Santacroce, Andrea Ballini, Luigi Leonardo Palese
Summary: The availability of drugs capable of blocking microorganism replication is a major accomplishment in medicine, but the increasing number of resistant strains poses a significant challenge for infectious disease treatment. Therefore, the search for new potential ligands for pathogens' life cycle is a crucial research area today.
Article
Plant Sciences
Juan Vorster, Willem van der Westhuizen, Gedion du Plessis, Diana Marais, Francesca Sparvoli, Eleonora Cominelli, Emanuela Camilli, Marika Ferrari, Cinzia Le Donne, Stefania Marconi, Silvia Lisciani, Alessia Losa, Tea Sala, Karl Kunert
Summary: Proteases and protease inhibitors play important roles throughout the life cycle of plants, but their functions in seed development are not fully understood. Specific serine protease inhibitors may affect seed development and require further investigation.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Pharmacology & Pharmacy
Exequiel O. J. Porta, Jaime A. Isern, Karunakaran Kalesh, Patrick G. Steel
Summary: In this study, a new therapeutic target for the treatment of leishmaniasis was discovered using an activity-based protein profiling strategy to investigate serine proteases (SPs) in Leishmania parasites. Significant differences in the expression levels of active Leishmania serine hydrolases (SHs) were observed throughout the parasite's life cycle and between different species. Two targetable SPs were identified in Leishmania mexicana using quantitative proteomic mass spectrometry. The results of this study provide templates for the discovery of new drugs for the treatment of leishmaniasis.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Oncology
Amiram Sananes, Itay Cohen, Irit Allon, Oshrit Ben-David, Raghda Abu Shareb, Ksenia M. Yegodayev, David Stepensky, Moshe Elkabets, Niv Papo
Summary: Targeted therapies for prostate, breast, and ovarian cancers are based on their activity against primary tumors rather than their anti-metastatic activity. There is an urgent need for new agents targeting the metastatic process. Mesotrypsin and KLK6 are attractive putative targets for therapeutic intervention.
MOLECULAR ONCOLOGY
(2023)
Review
Medicine, General & Internal
Sara Paczek, Barbara Mroczko
Summary: Gastrointestinal cancers pose a significant global healthcare problem. The lack of specific symptoms in the early stages and limited diagnostic methods contribute to the high mortality rates. New potential biomarkers, such as serpins, are being explored as diagnostic and prognostic factors. This review focuses on the significance of serpins in the development and progression of oesophageal, gastric, pancreatic, and colorectal cancers. Selected serpins play important roles in the pathogenesis of these cancers and show promise as therapeutic targets, although further research is needed to understand the mechanisms involved.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Multidisciplinary Sciences
Alejandro Garcia, M. Estrella Santamaria, Isabel Diaz, Manuel Martinez
Summary: The study reveals that transcriptomic meta-analysis is an effective approach to uncover physiological processes in plant responses to herbivores. Analysis of Arabidopsis-thaliana interactions with pests highlights key issues in plant responses, such as the specificity of perception and signaling pathways, and the variability in secondary metabolic pathways.
SCIENTIFIC REPORTS
(2021)
Review
Plant Sciences
Phetole Mangena
Summary: Recombinant gene encoded protease inhibitors have been recognized as effective molecules to protect essential proteins in plants. This review examines recently published literature on recombinant protease inhibitors (RPIs) and highlights their unintended consequences in plants, other organisms, and the environment.
FRONTIERS IN PLANT SCIENCE
(2022)
Review
Biochemistry & Molecular Biology
Noora Barzkar, Zahoor Khan, Saeid Tamadoni Jahromi, Sajjad Pourmozaffar, Mohsen Gozari, Reza Nahavandi
Summary: Marine organisms are valuable sources of enzymes and their inhibitors with great therapeutic potential, particularly serine proteases and their inhibitors showing promise in biomedical applications. The uncontrolled activity of these proteases can lead to diseases, but their actions are regulated by serine protease inhibitors to maintain physiological functions.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Review
Cardiac & Cardiovascular Systems
Steven P. Grover, Nigel Mackman
Summary: Appropriate activation of coagulation requires a balance between procoagulant and anticoagulant proteins in blood. SERPIN anticoagulants function by forming irreversible inhibitory complexes with target coagulation proteases. Mutations in SERPIN family members can cause hereditary thrombophilias, and low plasma levels of SERPINs are associated with an increased risk of thrombosis. Engineered SERPINs hold potential as novel therapies for the treatment of thrombotic pathologies.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2022)
Article
Plant Sciences
Emmanuel Margolin, Matthew Verbeek, Warren de Moor, Ros Chapman, Ann Meyers, Georgia Schaefer, Anna-Lise Williamson, Edward Rybicki
Summary: This study demonstrates the successful expression of the viral glycoprotein of SARS-CoV-2 in plants through co-expression with the human lectin-binding chaperone calreticulin, providing important insights for the rapid development of vaccines.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Chemistry, Multidisciplinary
Oleg Babii, Sergii Afonin, Christian Diel, Marcel Huhn, Jennifer Dommermuth, Tim Schober, Serhii Koniev, Andrii Hrebonkin, Alexander Nesterov-Mueller, Igor V. Komarov, Anne S. Ulrich
Summary: Chemical modification of a bicyclic peptide scaffold led to the development of photoswitchable inhibitors based on diarylethene for serine protease Bos taurus trypsin 1 (T1). These inhibitors exhibited low nanomolar K-i values and a 20-fold activity modulation under irradiation. The study also demonstrated the feasibility of manipulating enzyme activity in time and space.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Parasitology
Edwin F. Sanchez-Lopez, Mariana G. Corigliano, Romina M. Albarracin, Valeria A. Sander, Ariel Legarralde, Sofia A. Bengoa-Luoni, Marina Clemente
PARASITES & VECTORS
(2019)
Article
Biochemistry & Molecular Biology
Mariana G. Corigliano, Romina M. Albarracin, Juan M. Vilas, Edwin F. Sanchez Lopez, Sofia A. Bengoa Luoni, Bin Deng, Inmaculada Farran, Jon Veramendi, Santiago J. Maiale, Valeria A. Sander, Marina Clemente
Article
Parasitology
Sofia A. Bengoa-Luoni, Mariana G. Corigliano, Edwin Sanchez-Lopez, Romina M. Albarracin, Ariel Legarralde, Agustina Ganuza, Marina Clemente, Valeria A. Sander
Review
Immunology
Valeria A. Sander, Edwin F. Sanchez Lopez, Luisa Mendoza Morales, Victor A. Ramos Duarte, Mariana G. Corigliano, Marina Clemente
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2020)
Review
Biotechnology & Applied Microbiology
Mariana G. Corigliano, Valeria A. Sander, Edwin F. Sanchez Lopez, Victor A. Ramos Duarte, Luisa F. Mendoza Morales, Sergio O. Angel, Marina Clemente
Summary: Hsp90, known as heat shock proteins, can serve as immunomodulators to activate cellular functions for designing novel vaccines against infectious diseases. This review summarizes the adjuvant properties of Hsp90s and their mechanisms in activating and modulating professional antigen-presenting cells.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Plant Sciences
Edwin F. Sanchez-Lopez, Mariana G. Corigliano, Sonia Oliferuk, Victor A. Ramos-Duarte, Maximiliano Rivera, Luisa F. Mendoza-Morales, Sergio O. Angel, Valeria A. Sander, Marina Clemente
Summary: Plant-expressed HSP90 protein acts as a potent adjuvant to improve immune effects of Toxoplasma gondii antigen SAG1. Fusion protein AtHsp81.2-SAG1(HC) showed significant enhancement in antibody levels and reduced parasite load in mice compared to control groups. Plant-expressed AtHsp81.2-SAG1(HC) can directly be used as a potent biotechnological system for immunogenic antigen expression against toxoplasmosis.
FRONTIERS IN PLANT SCIENCE
(2021)
Editorial Material
Veterinary Sciences
Hany M. Ibrahim, Valeria A. Sander
FRONTIERS IN VETERINARY SCIENCE
(2022)
Review
Parasitology
Luisa F. Mendoza-Morales, Valentina Lagorio, Mariana G. Corigliano, Edwin Sanchez-Lopez, Victor A. Ramos-Duarte, Marina Clemente, Valeria A. Sander
Summary: Ovine neosporosis is widely distributed worldwide and is associated with factors such as geographical latitude, age, and production system. More research is needed to better understand the epidemiology of this disease and to raise awareness of its importance.
Article
Parasitology
Luisa F. Mendoza Morales, Valentina Lagorio, Mariana Corigliano, Edwin Sanchez-Lopez, Victor A. Ramos Duarte, Ariel Legarralde, Agustina Ganuza, Marina Clemente, Valeria Sander
Summary: This study evaluated the impact of Neospora caninum infection on the placenta, and found that infection can lead to fetal loss and immune system disruption, as well as increased oxidative stress and cytokine expression, all of which may contribute to abortion and reproductive failures in cattle.
PARASITOLOGY RESEARCH
(2023)
Review
Veterinary Sciences
Valeria A. Sander, Mariana G. Corigliano, Marina Clemente
FRONTIERS IN VETERINARY SCIENCE
(2019)