Article
Dentistry, Oral Surgery & Medicine
Ilan Pinkhasov, Liron Kabakov, Carlos E. E. Nemcovsky, Miron Weinreb, Pnina Schlesinger, Omer Bender, Maayan Gal, Daniel Z. Z. Bar, Evgeny Weinberg
Summary: The aim of this study was to investigate the heterogeneity of ex vivo-cultured human mesenchymal stromal cells derived from different regions of the oral mucosa. Single-cell RNA sequencing analysis revealed distinct cell sub-populations, with a higher proportion of mesenchymal stem cells in the masticatory mucosa. These findings have important implications for understanding cell function and potential therapeutic interventions.
JOURNAL OF CLINICAL PERIODONTOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Maria Ageles de Pedro, Maria Pulido, Veronica alvarez, Federica Marinaro, Ana Maria Marchena, Francisco Miguel Sanchez-Margallo, Javier G. Casado, Esther Lopez
Summary: This study found that Menstrual blood-derived stromal cells (MenSCs) have a good survival capacity under acute hypoxia conditions and have potential applications in tissue regeneration through their secretome, making them a potential cell source for wound healing interventions.
MOLECULAR MEDICINE
(2023)
Article
Cell Biology
Beverly Brooks, Dominique Ebedes, Ahsan Usmani, Joaquin Vega Gonzales-Portillo, Daniel Gonzales-Portillo, Cesario Borlongan
Summary: Ischemic brain injury is a major cause of death worldwide with limited treatment options. Stem cell-based regenerative therapies, specifically mesenchymal stromal cells, show promise in stroke treatment, but further research is needed to fully understand their cell function.
Article
Cell Biology
Denise Zujur, Ziadoon Al-Akashi, Anna Nakamura, Chengzhu Zhao, Kazuma Takahashi, Shizuka Aritomi, William Theoputra, Daisuke Kamiya, Koichi Nakayama, Makoto Ikeya
Summary: We developed a stepwise differentiation method to generate matrix-rich cartilage spheroids from induced pluripotent stem cell-derived mesenchymal stem/stromal cells (iMSCs) via the induction of neural crest cells under xeno-free conditions. By using a thienoindazole derivative, TD-198946, we achieved enhanced chondrogenesis in iMSCs. Our findings provide a novel cell source for stem cell-based cartilage repair and offer the potential for biofabrication of larger cartilage tissues using chondrogenic spheroids and technologies such as Kenzan Bioprinting.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Cell Biology
Jun Ochiai, Larakaye Villanueva, Hope Niihara, Yutaka Niihara, Joan Oliva
Summary: This study developed a method to engineer multilayer adipose stem cell cell sheets (ASCCSs) using chemically defined/serum-free culture media, which can differentiate into osteoblasts and chondrocytes. The number of cells per cell sheet was estimated using cell sheet transmittance, providing important non-invasive information.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Li-Na Zhang, Di-Di Zhang, Lei Yang, Yu-Xuan Gu, Qiu-Ping Zuo, Hao-Yi Wang, Jia Xu, Dian-Xin Liu
Summary: Invasion and metastasis are key characteristics of malignant tumors, with epithelial-mesenchymal transition (EMT) recognized as the initial step. Cell fusion, particularly involving mesenchymal stromal/stem cells (MSCs), plays a critical role in cancer development and progression, potentially influencing tumor growth and metastasis.
Review
Cell & Tissue Engineering
Mesude Bicer, Graeme S. Cottrell, Darius Widera
Summary: As populations age, osteoporosis and osteoporosis-related fractures are becoming increasingly prevalent degenerative bone diseases. Three-dimensional cell culture technology may serve as an alternative therapeutic approach to tackle osteoporosis, allowing for more physiological expansion and differentiation of stem cells.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Biochemistry & Molecular Biology
Monika Sypecka, Aleksandra Bzinkowska, Dorota Sulejczak, Filip Dabrowski, Anna Sarnowska
Summary: Wharton's jelly (WJ) from the umbilical cord (UC) is a good source of mesenchymal stem/stromal cells (MSCs) for therapy. Protocols for UC transportation, cell isolation, cultivation, and banking should be unified and optimized. WJ-MSCs showed higher levels of cytokines and chemokines secretion compared to WJ bioptats, and exhibited neural markers when exposed to cerebrospinal fluid (CSF). A protocol for manufacturing an advanced-therapy medicinal product was developed based on the findings.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Immunology
Fen Zhang, Linxiao Fan, Qiuhong Liu, Shima Tang, Sainan Zhang, Lanlan Xiao, Lingjian Zhang, Qian Li, Nueraili Maihemuti, Lanjuan Li
Summary: MenSCs treatment can alleviate autoimmune hepatitis by inhibiting macrophages and M1 polarization in the liver tissue.
FRONTIERS IN IMMUNOLOGY
(2022)
Review
Cell Biology
Meryem Ouzin, Gesine Kogler
Summary: Mesenchymal stromal cells are widely used in regenerative medicine and translational research, but their heterogeneity and the lack of understanding of their molecular mechanisms pose challenges for clinical applications. This review focuses on the heterogeneity aspects related to tissue source and developmental origin, emphasizing the importance of generating homogeneous products for clinical use.
Article
Biochemistry & Molecular Biology
Natalie Vallant, Bynvant Sandhu, Karim Hamaoui, Maria Prendecki, Charles Pusey, Vassilios Papalois
Summary: This study compared the paracrine effects of MSC supernatants from different age and genotype sources on macrophages, revealing distinct patterns of inflammatory marker expression. These findings are crucial for preclinical and clinical studies utilizing MSCs for inflammation and allo-response mitigation in transplant patients.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Cell & Tissue Engineering
Davod Pashoutan Sarvar, Hosein Effatpanah, Parvin Akbarzadehlaleh, Karim Shamsasenjan
Summary: The study highlights the important role of MSC-derived extracellular vesicles (MSC-EVs) in hematopoietic regulation and immune response after transplantation. MSC-EVs directly fuse with hematopoietic stem cell membranes, affecting their fate, and regulate the proliferation and differentiation of hematopoietic stem cells through the release of various bioactive factors.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Multidisciplinary Sciences
Wendi Guo, Colleen Wu
Summary: Currently, there is a lack of universally accepted markers for isolating skeletal stem cells (SSCs). Therefore, researchers continue to use bone marrow-derived mesenchymal stem cells (BMSCs) as a model to study SSCs and multipotent mesenchymal progenitors (MMPs). However, the common isolation procedures for BMSCs often result in a high percentage of hematopoietic cells, which can complicate data interpretation. In this study, a method using low oxygen tension or hypoxia is described to selectively eliminate CD45+ cells in BMSC cultures, reducing hematopoietic contaminants and enhancing MMPs and putative SSCs.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2023)
Article
Health Care Sciences & Services
Suncica Kapor, Milica Vukotic, Tijana Suboticki, Dragoslava Dikic, Olivera Mitrovic Ajtic, Milica Radojkovic, Vladan P. Cokic, Juan F. Santibanez
Summary: The study found that HU inhibits the growth of BMMSC, causing senescence-like changes and affecting cellular differentiation and immunoregulatory functions.
JOURNAL OF PERSONALIZED MEDICINE
(2021)
Review
Cell Biology
Stefania Crippa, Ludovica Santi, Margherita Berti, Giada De Ponti, Maria Ester Bernardo
Summary: The human organism requires the production of approximately 1 trillion new blood cells per day through hematopoiesis in the bone marrow, regulated by the BM microenvironment and HSPCs. The BM niche, defined by interactions between HSPCs and non-hematopoietic cells, controls HSPC maintenance and orchestrates hematopoiesis according to the body's needs. Mesenchymal stromal cells (MSCs) play a key role in the BM niche by regulating HSPC homeostasis and impacting the outcome of hematopoietic stem cell transplantation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Pathology
Juliana Pierobon Gomes da Cunha, Rodrigo Goncalves, Fernando Silva, Fernando Nalesso Aguiar, Bruna Salani Mota, Bruna Bello Chequim, Jose Maria Soares, Edmund C. Baracat, Jose Roberto Filassi
Summary: This study investigated the correlation between the Residual Cancer Burden (RCB) index and overall survival (OS) and disease-free survival (DFS) in women undergoing neoadjuvant chemotherapy for breast cancer. The results showed a significant relationship between the RCB index and the risk of relapse and death, suggesting the potential of RCB classification as a prognostic indicator.
JOURNAL OF CLINICAL PATHOLOGY
(2023)
Article
Obstetrics & Gynecology
Silvia Cristiane Alvarinho Junqueira, Thais Regina de Mattos Lourenco, Jose Maria Soares Junior, Lucilia Carvalho da Fonseca, Edmund Chada Baracat, Jorge Milhem Haddad
Summary: This study compares the effects of anterior and posterior vaginal approaches in sacrospinous ligament fixation on anatomical structures and correlates them with body mass index (BMI). The results show no difference in the distances measured in the two approaches, while the distances from the fixation point to the pudendal artery and nerve are directly proportional to the BMI.
INTERNATIONAL UROGYNECOLOGY JOURNAL
(2023)
Editorial Material
Medicine, General & Internal
Luciana C. Delamuta, Pedro A. A. Monteleone, Edson S. Ferreira-Filho, Vanessa Heinrich-Oliveira, Jose Maria Soares-Junior, Edmund C. Baracat, Gustavo Arantes Rosa Maciel
Article
Medicine, General & Internal
Daniele Carvalho Calvano Mendes, Carlos Marino Cabral Calvano Filho, Natalia Garcia, Marcos Desiderio Ricci, Jose Maria Soares Junior, Katia Candido Carvalho, Edmund Chada Baracat
Summary: The expression levels of miR-96-5p and miR-182-5p have been found to be upregulated in breast cancer, while the protein expression of FOXO3A is decreased. This phenomenon is more prominent in triple-negative breast cancer, suggesting the important role of these molecules in the biology and clinical behavior of this tumor type. These findings provide new perspectives for alternative target therapy options for breast cancer patients expressing both FOXO3A and its regulatory miRNAs.
Article
Obstetrics & Gynecology
Taina Pezzin Rocha, Marina Paula Andres, Francisco Carmona, Edmund Chada Baracat, Mauricio Simoes Abrao
Summary: According to this retrospective study, deep endometriosis is associated with severe pain symptoms and the number of affected compartments influences the severity of symptoms and the chance of infertility.
REPRODUCTIVE SCIENCES
(2023)
Article
Medicine, General & Internal
Maria Candida P. Baracat, Edmund C. Baracat, Ricardo S. Simoes, Manuel J. Simoes, Gustavo A. R. Maciel, Ricardo Azziz, Jose Maria Soares Jr
Summary: Our study aimed to investigate the effects of hormonal and metabolic factors on endometrial quality and progesterone action. Subjects were recruited from an outpatient reproductive endocrinology clinic. Endometrial biopsies were performed before and after progesterone treatment, and metabolic and hormonal serum levels were evaluated. The endometrium of women with PCOS in the second phase showed histomorphometric abnormalities, which were associated with androgen and insulin levels despite progesterone supplementation.
Article
Obstetrics & Gynecology
J. Zangirolami-Raimundo, R. D. Raimundo, P. R. E. Silva Noll, W. S. dos Santos, C. Leone, E. C. Baracat, I. C. E. Sorpreso, J. M. Soares Junior
Summary: This study aimed to assess whether prior knowledge of computer use determines performance of virtual reality tasks by postmenopausal women and whether menopausal symptoms, sociodemographic factors, lifestyle and cognition modify or interfere with their performance. The study found that postmenopausal computer users performed better in virtual reality games than non-users, and computer users experienced more severe vasomotor symptoms. Age, cognitive function, and headache symptoms were the best predictors affecting the postmenopausal women's performance in virtual reality tasks.
Review
Genetics & Heredity
Ana Berquo Peleja Eller, Dani Ejzenberg, Pedro Augusto Araujo Monteleone, Jose Maria Soares Jr, Edmund Chada Baracat
Summary: This review aims to assess the relationship between serum vitamin D levels and in vitro fertilization (IVF) results. The selected 18 articles showed mixed results, with some indicating a positive correlation, some lacking any association, and one exhibiting a negative correlation. Non-Hispanic White patients may be more affected by vitamin D deficiency compared to Asian patients. Vitamin D levels may play a role in the follicular fluid levels and immune system, impacting embryo implantation and pregnancy.
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
(2023)
Review
Medicine, General & Internal
Giovanna Santos Cavalcanti, Katia Candido Carvalho, Cecilia da Silva Ferreira, Peter Andre Chedraui Alvarez, Pedro Augusto Araujo Monteleone, Edmund Chada Baracat, Jose Maria Soares Jr
REVISTA DA ASSOCIACAO MEDICA BRASILEIRA
(2023)
Article
Medicine, General & Internal
Alexandra Carolina Canonica, Angelica Castilho Alonso, Guilherme Carlos Brech, Mark Peterson, Natalia Mariana Silva Luna, Alexandre Leopold Busse, Wilson Jacob-Filho, Juliana Leme Rosa, Jose Maria Soares-Junior, Edmund Chada Baracat, Julia Maria D'Aandrea Greve
Summary: This study aimed to analyze the adaptation of older adults and middle-aged adults in a driving simulator, and identify predictors of safe performance. The results showed that age, sex, and distraction play a role in the adaptation process, and certain factors such as muscle strength and postural balance are important predictors of braking time.
Article
Medicine, General & Internal
Joji Ueno, Renato De Mayrinck Salgado, Dani Ejzenberg, Filomena Marilia Henriques Carvalho, Eduardo Carvalho de Arruda Veiga, Jose Maria Soares Junior, Edmund Chada Baracat
Summary: This study aims to evaluate the influence of time and endometrial injury on pregnancy success. The results showed that the pregnancy rate was higher in the group with an interval of less than 90 days compared to the group with an interval of more than 90 days.
REVISTA DA ASSOCIACAO MEDICA BRASILEIRA
(2023)
Review
Medicine, General & Internal
Lorena Ana Mercedes Lara Urbanetz, Jose Maria Soares Jr, Gustavo Arantes Rosa Maciel, Ricardo dos Santos Simoes, Maria Candida Pinheiro Baracat, Edmund Chada Baracat
Summary: PCOS is an endocrine disorder characterized by chronic anovulation, hyperandrogenism, and polycystic ovaries. The exposure to BPA, through various means such as food packaging and skin absorption, can interact with estrogen receptors and cause harmful changes in women's metabolic and reproductive function, leading to PCOS.
Review
Medicine, General & Internal
Eduardo Carvalho de Arruda Veiga, Jose Maria Soares Junior, Marise Samama, Fabio Ikeda, Luciana Semiao Francisco, Amanda Sartor, Lorena Ana Mercedes Lara Urbanetz, Edmund Chada Baracat, Joji Ueno
REVISTA DA ASSOCIACAO MEDICA BRASILEIRA
(2023)