Article
Cell Biology
Dominik Steiner, Lea Reinhardt, Laura Fischer, Vanessa Popp, Carolin Koerner, Carol Geppert, Tobias Baeuerle, Raymund E. Horch, Andreas Arkudas
Summary: The microvascular endothelial network is crucial for osteogenesis and bone tissue engineering. Endothelial progenitor cells (EPCs) have a strong angiogenic potential and can enhance the vascularization and tissue formation of scaffolds. This study investigated the effects of EPCs on the vascularization and bone formation of a hydroxyapatite and beta-tricalcium phosphate-fibrin scaffold. The results showed successful scaffold vascularization in all groups, and the EPC/MSC coimplantation group demonstrated bone formation. The arteriovenous loop was found to be an effective method for vascularization independent of scaffold cellularization with EPCs.
Article
Chemistry, Physical
Si-Han Wang, Shiao-Pieng Lee, Chung-Wei Yang, Chun-Min Lo
Summary: Magnesium alloys with coatings show potential for bone substitute alternatives, but surface modification remains a challenge. The structure and Mg2+ concentration of FHA-Mg scaffolds significantly influence cell behavior, with cells on the scaffolds demonstrating better proliferation and osteogenic differentiation compared to those on culture dishes.
Article
Engineering, Biomedical
Ho-Ying Wan, Rita Lih Ying Shin, Jack Chun Hin Chen, Marisa Assuncao, Dan Wang, Susie K. Nilsson, Rocky S. Tuan, Anna Blocki
Summary: The study demonstrated that supplementation of Dextran sulfate (DxS) in osteogenically induced cultures of human mesenchymal stem cells (MSCs) amplified the deposition of osteoblastic extracellular matrix (ECM). This enhancement led to increased collagen type I deposition and osteogenic gene expression, as well as enrichment in bone morphogenetic protein 2 (BMP-2), suggesting a potential role of DxS in promoting osteogenic differentiation by MSCs.
ACTA BIOMATERIALIA
(2022)
Review
Pharmacology & Pharmacy
Jinli Zhang, Zhihe Liu, Yang Luo, Xiaojian Li, Guowei Huang, Huan Chen, Aiguo Li, Shengnan Qin
Summary: Flavonoids have the potential to regulate the osteogenic differentiation of mesenchymal stem cells (MSCs), making them a promising therapeutic application in bone disorders and bone tissue engineered constructs.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Materials Science, Multidisciplinary
Mahboobeh Mahmoodi, Vida Haghighi, Marjan Mirhaj, Maryam Tavafoghi, Fatemeh Shams, Ali Darabi
Summary: The addition of CNTs-COOH to PCL/Kr scaffold significantly improved its mechanical properties and osteogenic characteristics, resulting in reduced fiber diameter, increased specific surface area, and enhanced osteoconductivity. The PCL/Kr/CNT scaffold showed excellent osteogenic differentiation in simulated body fluid and promoted mineral formation on the scaffold's surface for mesenchymal stem cells.
MATERIALS TODAY COMMUNICATIONS
(2021)
Article
Nanoscience & Nanotechnology
Hongzi Wu, Dehua Zhang, Jian Wang, Yongqi Li, Feng Mao, Yi Liao
Summary: This study investigated the biocompatibility and in vitro osteogenic differentiation ability of a new degradable polymer scaffold, providing references for osseous tissue engineering. PGS-M/n-HA scaffold, prepared using Poly-glycerolsebacate-maleic anhydride (PGS-M) and Nano-hydroxyapatite (n-HA), was analyzed for morphology, porosity, degradation rate, and mechanical property. Human bone marrow mesenchymal stem cells (HBMSCs) were cultured in various conditions to compare cell adhesion rate, viability, and alkaline phosphatase (AKP) activity. The results showed that PGS-M/n-HA scaffold exhibited high degradability, mechanical property, and biocompatibility, promoting the differentiation of HBMSCs into osteoblasts.
JOURNAL OF BIOMEDICAL NANOTECHNOLOGY
(2023)
Review
Cell Biology
Sandra Stamnitz, Aleksandra Klimczak
Summary: Mesenchymal stem cell-based therapies show promise in bone tissue regeneration, but cell tracking and maintenance in the injury site pose challenges. Seeding cells onto a biocompatible scaffold may offer a potential solution. The development of bone tissue engineering involves optimizing various variables like stem cell source, osteogenic molecular factors, scaffold design, and suitable animal models.
Review
Cell Biology
Kangkang Zha, Yue Tian, Adriana C. Panayi, Bobin Mi, Guohui Liu
Summary: Bone tissue engineering offers a new approach to treating bone defects, with mesenchymal stem cells being a promising seed cell due to their functions in osteogenic differentiation. Improving methods of origin selection, culture conditions, and biophysical stimulation can enhance MSC osteogenesis and contribute to the development of MSC-based bone tissue engineering.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Chemistry, Physical
Federica Riva, Nora Bloise, Claudia Omes, Gabriele Ceccarelli, Lorenzo Fassina, Rossella Elena Nappi, Livia Visai
Summary: Recent studies have found stem cells (hFF-MSCs) in ovarian follicular fluid (hFF) with similar properties to MSCs derived from other tissues. These hFF-MSCs, isolated from waste matter after oocyte retrieval, are an untapped source of stem cell materials. This study evaluates the osteogenic capacity of hFF-MSCs seeded on bioglass 58S-coated titanium and their suitability for bone tissue engineering. The findings suggest that hFF-MSCs cultured on bioglass-coated titanium exhibit enhanced osteogenic differentiation, indicating their potential in regenerative medicine and as an alternative to hBM-MSCs in bone tissue engineering models.
Article
Biotechnology & Applied Microbiology
Xu Sun, Shude Yang, Shuang Tong, Shu Guo
Summary: Titanium and titanium alloys are commonly used materials for bone tissue engineering. However, due to their bio-inert nature, they do not match the elastic modulus of bone. Surface modifications have been proposed to address this issue. In this study, a compound scaffold called Gr-Ti was prepared with exosomes derived from adipose-derived stem cells (ADSCs). The results showed that Gr-Ti scaffolds had low toxicity and good biocompatibility, promoting the adhesion and osteogenic differentiation of ADSCs. The addition of ADSC-derived exosomes significantly increased the mRNA levels of osteogenic markers and improved the bone properties of the scaffold. This study provides a theoretical basis for the development of new clinical bone repair materials.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Review
Engineering, Biomedical
Ziyi Feng, Meiqi Jin, Junzhi Liang, Junning Kang, Huazhe Yang, Shu Guo, Xiaoting Sun
Summary: Bone damage may require bone grafting and bone tissue engineering can be an alternative strategy. Mesenchymal stem cells (MSCs) are important for tissue engineering due to their ability to differentiate into various cell types. The regulation of MSCs differentiation and the role of mitochondria in this process are crucial for efficient bone regeneration.
ACTA BIOMATERIALIA
(2023)
Article
Biochemistry & Molecular Biology
Esen Sayin, Erkan Turker Baran, Ahmed Elsheikh, Vivek Mudera, Umber Cheema, Vasif Hasirci
Summary: It was found that the 3D culture of MSCs in collagen/silk fibroin scaffolds could significantly enhance the expressions of OSX and VEGFA, and result in improved tensile mechanical properties compared to 2D culture, with different effects observed under different oxygen environments.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Pharmacology & Pharmacy
Sumeyra Nur Fuerkaiti, Anil Sera Cakmak, Cagatay Karaaslan, Menemse Gumusderelioglu
Summary: This study demonstrated that silencing Noggin using siRNA induces osteogenic differentiation in reduced doses of BMP-2 for scaffold-based bone regeneration. The siNoggin-transfected cells showed enhanced osteogenic effects and mineralization, suggesting a potential safe therapeutic approach for tissue regeneration.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2022)
Article
Nanoscience & Nanotechnology
Seok Hyun Lee, Sangheon Jeon, Xiaoxiao Qu, Moon Sung Kang, Jong Ho Lee, Dong-Wook Han, Suck Won Hong
Summary: Conventional bioinert bone grafts often fail in osseointegration due to their low bioactivity. MXene nanoparticles have recently shown promising results as a rising material for bone grafts or scaffolds, thanks to their unique atomic structure. In this study, the researchers produced MXene nanoparticles-integrated nanofibrous matrices for bone tissue engineering. The results demonstrated that the scaffolds supported the growth and survival of preosteoblasts, and promoted spontaneous osteodifferentiation.
Article
Materials Science, Multidisciplinary
Xiping Jiang, Yunfan Kong, Mitchell Kuss, Joel Weisenburger, Hani Haider, Robert Harms, Wen Shi, Bo Liu, Wen Xue, Jianghu Dong, Jingwei Xie, Philipp Streubel, Bin Duan
Summary: This study presents a novel therapeutic method that combines three-dimensional (3D) bioprinting and melt electrospinning writing techniques to regenerate the tendon-to-bone interface. The use of 3D-bioprinted pre-differentiated autologous adipose-derived mesenchymal stem cells (ADMSC) generated biomimetic multilayered scaffolds that recapitulated the compositional and cellular structures of the interface. In vivo studies demonstrated the superior histological score and improved collagen organization of the scaffold with spatially differentiated autologous ADMSCs.
APPLIED MATERIALS TODAY
(2022)
Review
Pharmacology & Pharmacy
Cristiana-Ioana Bercea, Graeme S. Cottrell, Francesco Tamagnini, Alister J. McNeish
Summary: Hypertension is characterised by impaired vasodilation, which can be improved by omega-3 polyunsaturated fatty acids such as DHA and EPA. These fatty acids also have vasodilatory effects on vascular smooth muscle cells (VSMCs) by opening potassium channels and possibly voltage-activated potassium channels, leading to hyperpolarisation and relaxation. Structural manipulation of omega-3 PUFAs may lead to novel therapeutic strategies.
BRITISH JOURNAL OF PHARMACOLOGY
(2021)
Article
Cell Biology
Vasco C. Silveirinha, Hong Lin, Shota Tanifuji, Sumiko Mochida, Graeme S. Cottrell, Helena Cimarosti, Gary J. Stephens
Summary: SUMOylation is an important post-translational modification process that involves the covalent attachment of SUMO protein to target proteins. The study found that SUMO-1 protein can modulate the function of the Cav2.2 voltage-gated calcium channel, affecting neurotransmitter release and providing novel targets for SUMOylation pathways.
Review
Medicine, Research & Experimental
Nazmul Haque, Darius Widera, Vijayendran Govindasamy, Pratiwi Soesilawati, Noor Hayaty Abu Kasim
Summary: Cell-based regenerative therapies have the potential to treat non-communicable and degenerative diseases. Extracellular vesicles (EVs), which contain miRNAs, mRNAs, and proteins, play a role in intercellular communication. However, the regenerative potential of these molecules is still debated.
CURRENT MOLECULAR MEDICINE
(2022)
Review
Biochemistry & Molecular Biology
Dirk Roosterman, Graeme Stuart Cottrell
Summary: The Citric Acid Cycle, initially proposed in 1937 by Sir H. A Krebs, is a unidirectional cycle crucial for the degradation of lactic acid and energy production. The heart lactate dehydrogenase center · proton-linked monocarboxylate transporter 1 complex plays a key role in catalyzing the initial step of the cycle by oxidizing lactate to pyruvate. By incorporating oxaloacetic acid into the cycle, anabolic processes can be integrated, allowing organisms to recover ATP more efficiently.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell & Tissue Engineering
Noridzzaida Ridzuan, Norashikin Zakaria, Darius Widera, Jonathan Sheard, Mitsuru Morimoto, Hirofumi Kiyokawa, Seoparjoo Azmel Mohd Isa, Gurjeet Kaur Chatar Singh, Kong-Yong Then, Ghee-Chien Ooi, Badrul Hisham Yahaya
Summary: This study demonstrated the potential anti-inflammatory effects of human umbilical cord mesenchymal stem cell (hUC-MSC)-derived extracellular vesicles (EVs) in a rat model of COPD. Both hUC-MSCs and EVs were found to effectively ameliorate COPD-induced inflammation, suggesting that EVs could serve as a new cell-free-based therapy for the treatment of COPD.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Biochemistry & Molecular Biology
Evgenii Skurikhin, Pavel Madonov, Olga Pershina, Natalia Ermakova, Angelina Pakhomova, Darius Widera, Edgar Pan, Mariia Zhukova, Lubov Sandrikina, Andrey Artamonov, Alexander Dygai
Summary: In patients with idiopathic pulmonary fibrosis, the concentration of hyaluronic acid in the lungs increases, which can be broken down by hyaluronidase. The conjugate pHD effectively reduces fibrosis levels and when combined with spiperone, enhances anti-inflammatory and antifibrotic effects.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Enrico C. Torre, Mesude Bicer, Graeme S. Cottrell, Darius Widera, Francesco Tamagnini
Summary: Adipose-derived mesenchymal stromal cells (ASCs) have the potential to differentiate into various cell types and exhibit spontaneous Ca2+ oscillations. The effects of differentiation treatments on ASCs' Ca2+ dynamics, in terms of oscillation fraction and frequency, were examined in this study. Both adipogenic and osteogenic differentiation resulted in reduced Ca2+ dynamics, with adipogenic differentiation showing a more pronounced effect. Changes in Ca2+ oscillations were observed as early as 7 days in vitro (DIV). Further research is needed to understand the molecular and mechanistic correlates of these changes for the development of interventions in regenerative medicine.
Article
Biochemistry & Molecular Biology
Olga Pershina, Natalia Ermakova, Angelina Pakhomova, Darius Widera, Edgar Pan, Mariia Zhukova, Elena Slonimskaya, Sergey G. Morozov, Aslan Kubatiev, Alexander Dygai, Evgenii G. Skurikhin
Summary: This study found that breast cancer patients can be divided into two groups based on the frequency of ALDH1 cells, corresponding to different types of stem cells. Additionally, epithelial precursors associated with a positive effect of chemotherapy and remission in breast cancer patients were found in normal breast tissue.
Article
Respiratory System
Evgenii Skurikhin, Olga Pershina, Mariia Zhukova, Darius Widera, Edgar Pan, Angelina Pakhomova, Vyacheslav Krupin, Natalia Ermakova, Victoria Skurikhina, Lubov Sandrikina, Sergey Morozov, Aslan Kubatiev, Alexander Dygai
Summary: The study demonstrated that spiperone can promote pulmonary endothelium regeneration and reduce the occurrence of pulmonary emphysema. It achieves its therapeutic effects by influencing the migration and proliferation of endothelial cells.
INTERNATIONAL JOURNAL OF CHRONIC OBSTRUCTIVE PULMONARY DISEASE
(2021)
Article
Cell Biology
Kahdr A. Alatawi, Divyashree Ravishankar, Pabitra H. Patra, Alexander P. Bye, Alexander R. Stainer, Ketan Patel, Darius Widera, Sakthivel Vaiyapuri
Summary: 1,8-cineole has shown inhibitory effects on platelet activation, thrombus formation, and hemostasis, affecting platelet signaling pathways, granule secretion, and intracellular calcium mobilization. It may act as a potential therapeutic agent to control unwarranted platelet reactivity under various pathophysiological settings.
Article
Biochemistry & Molecular Biology
Evgenii G. Skurikhin, Olga Pershina, Natalia Ermakova, Angelina Pakhomova, Darius Widera, Mariia Zhukova, Edgar Pan, Lubov Sandrikina, Lena Kogai, Nikolai Kushlinskii, Sergey G. Morozov, Aslan Kubatiev, Alexander Dygai
Summary: CD8(+) T-lymphocytes play a crucial role in the immune response against lung cancer. Reprogrammed T-lymphocytes, using MEK inhibitors and PD-1 blockers, show increased antitumor activity. This reprogramming approach demonstrates significant antitumor effects both in vitro and in vivo.
Article
Biochemistry & Molecular Biology
Evgenii G. Skurikhin, Natalia Ermakova, Mariia Zhukova, Olga Pershina, Edgar Pan, Angelina Pakhomova, Lena Kogai, Victor Goldberg, Elena Simolina, Victoria Skurikhina, Darius Widera, Aslan Kubatiev, Sergey G. Morozov, Nikolai Kushlinskii, Alexander Dygai
Summary: This study analyzed circulating tumor cells (CTCs) and cancer stem cells (CSCs) in patients with small cell lung cancer (SCLC) to search for new diagnostic and prognostic markers and novel approaches to improve the treatment of the disease. The results showed heterogeneity of CTCs and CSCs populations in blood, as well as resistance to chemotherapy. Additionally, a new approach using reprogrammed T-lymphocytes targeting CTCs and CSCs was demonstrated.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Evgenii G. G. Skurikhin, Olga Pershina, Natalia Ermakova, Angelina Pakhomova, Mariia Zhukova, Edgar Pan, Lubov Sandrikina, Darius Widera, Lena Kogai, Nikolai Kushlinskii, Aslan Kubatiev, Sergey G. G. Morozov, Alexander Dygai
Summary: This study demonstrates the potential of using a combination of MEK inhibitor and PD-1 blocker to reprogram human CD8(+) T-cells and target lung cancer cells, resulting in antimetastatic activity, decreased cancer cell and cancer stem cell numbers in the lungs, and increased T-cell numbers in the blood. These findings suggest a new approach to targeted lung cancer therapy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell & Tissue Engineering
Atiyeh Asadpour, Badrul Hisham Yahaya, Katrina Bicknell, Graeme S. Cottrell, Darius Widera
Summary: The direct-to-consumer industry for secretome-based therapies is growing rapidly without appropriate regulation and guidelines. Many businesses are offering secretome-based interventions without sufficient evidence, putting patients at risk and potentially damaging the credibility of the field.
STEM CELL RESEARCH & THERAPY
(2023)
