Review
Biotechnology & Applied Microbiology
Lisette Van Os, Britta Engelhardt, Olivier T. Guenat
Summary: In order to develop new therapies for infections, understanding the human immune system during infection is crucial. Organ-on-chip models have proven to be valuable tools for tissue modeling, and incorporating an immune component is necessary to simulate complex biological responses. This tutorial review provides a practical guide on developing an organ-on-chip model to investigate immune cell migration during infection, including the design of the chip, establishment of chemotactic gradients, and incorporation of different cell types. The review also highlights the importance of the extracellular matrix in accurately modeling the interstitial space for immune cell migration.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Review
Biotechnology & Applied Microbiology
Lucie A. Low, Christine Mummery, Brian R. Berridge, Christopher P. Austin, Danilo A. Tagle
Summary: OoCs, also known as microphysiological systems or 'tissue chips', have the potential to be informative at multiple stages of drug discovery and development, providing insights into normal human organ function and disease pathophysiology, as well as predicting the safety and efficacy of investigational drugs in humans. The field of OoCs has seen significant advances in recent years, but there are still challenges and opportunities that need to be addressed to fully realize their potential for translational research.
NATURE REVIEWS DRUG DISCOVERY
(2021)
Review
Cell Biology
Louis Jun Ye Ong, Xiwei Fan, Antonia Rujia Sun, Lin Mei, Yi-Chin Toh, Indira Prasadam
Summary: Osteoarthritis (OA) is a prevalent disease worldwide, affecting over 20% of the population and leading to decreased quality of life for patients. Due to the complexity of mimicking joint tissue's physiological environment, the study of OA pathophysiology primarily relies on animal models. However, recent advancements in microfluidic organ-on-chip (OoC) systems have shown promise in replicating and manipulating tissue physiological environments. By adapting these techniques, it is possible to create human-specific in vitro models that accurately capture the cellular processes involved in OA. This review provides a comprehensive summary of various microfluidic platforms used to mimic joint microenvironments, with implications for future platform design iterations.
Review
Pharmacology & Pharmacy
Zohreh Izadifar, Alexandra Sontheimer-Phelps, Bob A. Lubamba, Haiqing Bai, Cicely Fadel, Anna Stejskalova, Alican Ozkan, Queeny Dasgupta, Amir Bein, Abidemi Junaid, Aakanksha Gulati, Gautam Mahajan, Seongmin Kim, Nina T. LoGrande, Arash Naziripour, Donald E. Ingber
Summary: Studying mucus biology has been challenging due to the lack of physiologically relevant human in vitro models. However, recent progress has been made in the development of human organ-on-a-chip microfluidic culture models, focusing on the lung, colon, small intestine, cervix, and vagina. These models can reconstitute epithelial tissue barriers and physiologically relevant mucus layers, allowing for the study of mucus composition, mechanics, and structure in a biomimetic environment.
ADVANCED DRUG DELIVERY REVIEWS
(2022)
Review
Biotechnology & Applied Microbiology
Yi Yang, Yin Chen, Liang Wang, Shihui Xu, Guoqing Fang, Xilin Guo, Zaozao Chen, Zhongze Gu
Summary: The combination of organ-on-a-chip technology and physiologically based pharmacokinetic modeling has the potential to improve drug development and personalized medicine.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biophysics
Dongxin Xu, Jiaru Fang, Mingyue Zhang, Hao Wang, Tao Zhang, Tian Hang, Xi Xie, Ning Hu
Summary: This study introduces a novel platform that achieves synchronized intracellular and extracellular recordings of neonatal rat cardiomyocytes by low-voltage three-dimensional nanoroded electroporation, enabling high-throughput large-scale synchronous electrophysiological studies. Recorded signals contain typical EAPs and IAPs with good synchronicity in spatiotemporal dimensions, bridging the correlation between both signals in experimental and simulated ways.
BIOSENSORS & BIOELECTRONICS
(2021)
Review
Chemistry, Multidisciplinary
Lingyu Sun, Feika Bian, Dongyu Xu, Yuan Luo, Yongan Wang, Yuanjin Zhao
Summary: This article provides an overview of the advances in biomaterials for the construction of organs-on-chips. It introduces the composition, structure, and fabrication techniques of biomaterials, and focuses on their functions and applications in organs-on-chips systems. The use of biomaterial-based organs-on-chips as alternative animal models and the challenges and prospects of further functionalization are also discussed.
MATERIALS HORIZONS
(2023)
Article
Biophysics
Anna Grazia Monteduro, Silvia Rizzato, Giusi Caragnano, Adriana Trapani, Gianluigi Giannelli, Giuseppe Maruccio
Summary: Current in-vitro 2D cultures and animal models have limitations in recapitulating human physiology, but microphysiological systems and organ-on-chip technologies are emerging as novel tools for high-throughput research and drug development. This review provides an introduction to major themes in this field and discusses the significance, limitations, and future prospects of these technologies.
BIOSENSORS & BIOELECTRONICS
(2023)
Review
Pharmacology & Pharmacy
Berivan Cecen, Christina Karavasili, Mubashir Nazir, Anant Bhusal, Elvan Dogan, Fatemeh Shahriyari, Sedef Tamburaci, Melda Buyukoz, Leyla Didem Kozaci, Amir K. Miri
Summary: Organ-on-a-chip technology is utilized for testing small-molecule drugs, with a focus on simulating interactions between drugs and the body. The development of multi-organ-on-a-chip (MOC) technology aims to replicate various organs involved in drug-body interactions. Current research in MOCs is particularly concentrated on studying the dynamic processes of drug absorption, distribution, metabolism, and excretion.
Article
Engineering, Multidisciplinary
Nan Xia, Yujuan Zhu, Rui Liu, Weiwei Chen, Yuanjin Zhao, Lingyun Sun
Summary: A novel monitoring system was developed by integrating decellularized lotus with microfluidic chips to induce and monitor cell alignment. The decellularized lotus petioles showed high cytocompatibility and facilitated the alignment and differentiation of neural cells. The system was able to efficiently monitor cell alignment and improve neural function. It provides a cost-effective and high-throughput approach for future research on neuronal cells.
COMPOSITES PART B-ENGINEERING
(2023)
Article
Biophysics
Jonathan Sabate del Rio, Jooyoung Ro, Heejeong Yoon, Tae-Eun Park, Yoon-Kyoung Cho
Summary: Organs-on-chips (OoCs) are biomimetic in vitro systems that mimic the physicochemical microenvironments, physiologies, and key functional units of specific human organs using microfluidic cell cultures. These systems have the potential to replace animal models, enable personalized medicine, and require continuous monitoring of quality parameters. Integration of biosensing technologies into OoCs allows for monitoring of their physiologies, functions, and microenvironments. Future directions involve the application of artificial intelligence for process optimization, self-regulation, and data analysis in OoCs and cyber-physical systems.
BIOSENSORS & BIOELECTRONICS
(2023)
Review
Multidisciplinary Sciences
Jintao Li, Jie Chen, Hua Bai, Haiwei Wang, Shiping Hao, Yang Ding, Bo Peng, Jing Zhang, Lin Li, Wei Huang
Summary: Microfluidic-based organs-on-chips (OoCs) are an advanced technology in biomedical and chemical research, offering unique properties for biomedical applications. However, the amount of data generated by OoC systems has surpassed manual analysis capabilities. Deep learning, a new area in machine learning, can automatically analyze big data and has successful applications in various fields. The integration of deep learning in OoCs shows great potential for drug development, disease modeling, and personalized medicine.
Review
Genetics & Heredity
Donald E. Ingber
Summary: This Review discusses the types of single and multiple human organ-on-a-chip (organ chip) microfluidic devices and their diverse applications for disease modeling, drug development, and personalized medicine. It also addresses the challenges that must be overcome for organ chips to reach their full potential and discusses recent advances in the field.
NATURE REVIEWS GENETICS
(2022)
Review
Endocrinology & Metabolism
Eisa Tahmasbpour Marzouni, Catharyn Stern, Andrew Henrik Sinclair, Elena Jane Tucker
Summary: This article discusses the importance and challenges of using a combination of stem cells and gene editing technology for treating infertility, as well as the potential role of organ-on-a-chip models in human reproduction.
Article
Chemistry, Analytical
Mees N. S. de Graaf, Aisen Vivas, Andries D. van der Meer, Christine L. Mummery, Valeria V. Orlova
Summary: Organ-on-chip devices are used to simulate the tissue microenvironment of cells in intact organs. This study presents a method for multiplexing microfluidic channels in these devices, along with custom software for system operation. The approach is also suitable for culture medium recirculation, which is important for cost considerations.
Article
Multidisciplinary Sciences
Jiaru Fang, Dong Liu, Dongxin Xu, Qianni Wu, Hongbo Li, Ying Li, Ning Hu
Summary: This study developed an integrated Au-nanoroded biosensing and regulating platform for investigating the photothermal therapy of cardiac bradyarrhythmia. The platform allows real-time monitoring of the electrophysiological state of cardiomyocytes and restoration of normal rhythm. Compared to conventional strategies, the photothermal strategy is more effective and convenient.
Article
Biophysics
Dongxin Xu, Jiaru Fang, Moran Yadid, Mingyue Zhang, Hao Wang, Qijian Xia, Hongbo Li, Nan Cao, Tal Dvir, Ning Hu
Summary: Intracellular recording of action potentials is crucial for studying disease mechanisms and electrophysiological studies. Current strategies include 3D nanoelectrodes and transient electroporation, but both have limitations. In this study, an advanced cell-based biosensing platform using electroporation is proposed, which provides consistent, high-quality recordings and allows real-time feedback.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Biophysics
Mingyue Zhang, Dongxin Xu, Jiaru Fang, Hongbo Li, Ying Li, Chuan Liu, Nan Cao, Ning Hu
Summary: The study develops a high-throughput, sensitive, and stable biosensing platform to dynamically and quantitatively assess the evolution of electroporated cell membrane. The platform explores and characterizes the processes of perforation, stabilization, and resealing of the cell membrane through intracellular recordings of action potentials.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Chemistry, Multidisciplinary
Dongxin Xu, Jiaru Fang, Mingyue Zhang, Qijian Xia, Hongbo Li, Ning Hu
Summary: New strategies for intracellular electrophysiology using nanotemplate electrodes provide sensitive and prolonged recording of action potentials, leading to significant advances in the field of cardiology and neuroscience.
Article
Immunology
Xiaonan Zhou, Kai Zhu, Yiqiu Zhang, Yang Ming, Dai Shi, Hui Tan, Bitao Xiang, Shichao Zhu, Dengfeng Cheng, Hao Lai, Chunsheng Wang, Guobing Liu
Summary: This study investigated the feasibility of a pre-targeted imaging strategy using the cycloaddition between Tz and TCO for evaluating CD11b expression in inflammatory AA. The results showed that this strategy allowed for the detection of inflammation in AA mice with high specificity and affinity.
JOURNAL OF INFLAMMATION RESEARCH
(2022)
Article
Chemistry, Multidisciplinary
Dongxin Xu, Hongbo Xiao, Shuzhe Wang, Hongbo Li, Hui-Jiuan Chen, Chuan Liu, Ning Hu
Summary: The preclinical assessment of efficacy and safety is crucial in cardiovascular drug development to ensure effective prevention and treatment of cardiovascular disease. A biosensing platform involving a multichannel electrode array device and a universal mechanical beating detection system has been proposed, which can detect subtle changes in mechanical beating signals induced by cardiovascular drugs and demonstrate high performance in pharmacological assessment.
Article
Biophysics
Diming Zhang, Yuting Xiang, Quchao Zou, Kai Zhu, Ning Hu
Summary: The assessment of drug cardiotoxicity using the electrical and mechanical properties of cardiomyocytes is crucial. Traditional recording strategies have limitations, but a new multimodal microelectrode biosensing system allows for simultaneous and dynamic monitoring of electromechanical signals from single cardiomyocytes.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Chemistry, Multidisciplinary
Dongxin Xu, Jiaru Fang, Hao Wang, Xinwei Wei, Jinhu Yang, Hongbo Li, Tao Yang, Ying Li, Chuan Liu, Ning Hu
Summary: In this study, a nanotrapped microelectrode based on a porous PET membrane was developed to improve cell-electrode coupling and intracellular recording performance. The nanotraps and nanoedge structure enhance the protrusion of the cell membrane and provide effective electroporation, allowing for longer intracellular access and consecutive electroporation events.
Article
Chemistry, Multidisciplinary
Jiaru Fang, Dongxin Xu, Hao Wang, Jin Wu, Ying Li, Tao Yang, Chuan Liu, Ning Hu
Summary: Electrophysiology is a unique biomarker for disease investigation or drug assessment in electrogenic cells. Vertical nanoelectrode arrays have been successful in achieving high-quality intracellular electrophysiological study in electrogenic cells. However, achieving high success rate and high-quality, long-term intracellular recording using inexpensive nanostructures remains a challenge. In this study, a scalable and robust hollow nanopillar electrode was developed to enhance intracellular recording of cardiomyocytes. Regulation of the nanostructure geometry proved to be a powerful strategy in enhancing intracellular recording.
Article
Chemistry, Analytical
Hao Wang, Quchao Zou, Yuting Xiang, Jinhu Yang, Zhongyuan Xu, Wenjian Yang, Yue Wu, Jin Wu, Dong Liu, Ning Hu, Diming Zhang
Summary: Researchers have developed a colorimetric plate reader based on smartphones and tablets, which has intelligent and dynamic light modulation capabilities. Compared to traditional plate readers and smartphone-based systems, this system exhibits higher sensitivity, lower detection limits, and broader detection ranges.
Article
Chemistry, Multidisciplinary
Jiaru Fang, Yuxiang Pan, Jiarong Xu, Dongxin Xu, Hongbo Li, Chuan Liu, Ning Hu
Summary: An integrated cardiomyocyte-based biosensing platform has been developed to record action potential and evaluate the quality of electroporation. This platform shows potential for drug delivery therapy and pathology research in cardiology.
Article
Chemistry, Multidisciplinary
Kai Zhu, Tao Yan, Chunlian Qin, Yuxiang Pan, Jun Li, Hao Lai, Dongxin Xu, Chunsheng Wang, Ning Hu
Summary: This study presents the development of a three-dimensional cardiomyocyte-nanobiosensing system that can specifically recognize drug subgroups, providing an accurate and sensitive platform for drug screening in cardiovascular diseases.
Article
Chemistry, Analytical
Ning Hu, Hao Wan
Article
Nanoscience & Nanotechnology
Xingxing Liu, Dongxin Xu, Jiaru Fang, Yuheng Liao, Mingyue Zhang, Hongbo Li, Wenjian Yang, Yue Wu, Zhongyuan Xu, Ning Hu, Diming Zhang
Summary: With the development of micro/nanotechnologies, researchers have developed multielectrode arrays (MEAs) with different three-dimensional (3D) micro/nanostructures for intracellular action potential recording of cardiomyocytes. However, the effect of 3D micro/nanostructure density on intracellular recording has not been fully explored. In this study, 3D tunable nanodensity electrode arrays (TNDEA) were fabricated to investigate the impact of nanodensity regulation on intracellular biosensing. The low-nanodensity TNDEA achieved improved signal quality of intracellular potentials, including amplitude, signal-to-noise ratio (SNR), recording duration, and recording yield, compared to the high-nanodensity TNDEA. This 3D nanodensity regulating strategy has the potential to serve as a powerful electrophysiological research tool in the biomedical field.
Review
Nanoscience & Nanotechnology
Yi Zhang, Ning Hu, Jiajie Xu, Zhen Wang
Summary: DNA programming, based on base complementary pairing and Boolean operations, has practical applications in multidisciplinary cutting-edge research. DNA logic circuits play a crucial role in biomedical fields, enabling autonomous assessment and stable visual signal outputs.
