Deregulation of methanol metabolism reverts transcriptional limitations of recombinant Pichia pastoris (Komagataella spp) with multiple expression cassettes under control of the AOX1 promoter

Engineering strategies for enhanced production of protein and bio-products in Pichia pastoris : A review

(2018) Zhiliang Yang et al.   BIOTECHNOLOGY ADVANCES

Methanol independent induction in Pichia pastoris by simple derepressed overexpression of single transcription factors

(2018) Thomas Vogl et al.   BIOTECHNOLOGY AND BIOENGINEERING

Increased dosage of AOX1 promoter-regulated expression cassettes leads to transcription attenuation of the methanol metabolism in Pichia pastoris

(2017) Elena Cámara et al.   Scientific Reports

Droplet digital PCR-aided screening and characterization ofPichia pastorismultiple gene copy strains

(2016) Elena Cámara et al.   BIOTECHNOLOGY AND BIOENGINEERING

Mit1 Transcription Factor Mediates Methanol Signaling and Regulates the Alcohol Oxidase 1 (AOX1) Promoter inPichia pastoris

(2016) Xiaolong Wang et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

High-level expression of Proteinase K from Tritirachium album Limber in Pichia pastoris using multi-copy expression strains

(2016) Hu Yang et al.   PROTEIN EXPRESSION AND PURIFICATION

PpNrg1 is a transcriptional repressor for glucose and glycerol repression of AOX1 promoter in methylotrophic yeast Pichia pastoris

(2015) Xiaolong Wang et al.   BIOTECHNOLOGY LETTERS

Systems-level organization of yeast methylotrophic lifestyle

(2015) Hannes Rußmayer et al.   BMC BIOLOGY

Pichia pastoris as an expression host for membrane protein structural biology

(2015) Bernadette Byrne   CURRENT OPINION IN STRUCTURAL BIOLOGY

Expression of enzymes for the usage in food and feed industry with Pichia pastoris

(2015) Sebastian C. Spohner et al.   JOURNAL OF BIOTECHNOLOGY

Protein expression in Pichia pastoris: recent achievements and perspectives for heterologous protein production

(2014) Mudassar Ahmad et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Trm1p, a Zn(II)2Cys6-type transcription factor, is essential for the transcriptional activation of genes of methanol utilization pathway, in Pichia pastoris

(2014) Umakant Sahu et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Improved production of a recombinant Rhizomucor miehei lipase expressed in Pichia pastoris and its application for conversion of microalgae oil to biodiesel

(2014) Jinjin Huang et al.   Biotechnology for Biofuels

Direct Evaluation of the Effect of Gene Dosage on Secretion of Protein from Yeast Pichia pastoris by Expressing EGFP

(2014) Hailong Liu et al.   JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY

Recombinant protein subunit vaccine synthesis in microbes: a role for yeast?

(2014) Roslyn M. Bill   JOURNAL OF PHARMACY AND PHARMACOLOGY

Glucose-methanol co-utilization in Pichia pastoris studied by metabolomics and instationary 13C flux analysis

(2013) Joel Jordà et al.   BMC Systems Biology

Recombinants proteins for industrial uses: utilization of Pichia pastoris expression system

(2013) Claudia Rabert et al.   BRAZILIAN JOURNAL OF MICROBIOLOGY

Enhancement of lipase r27RCL production in Pichia pastoris by regulating gene dosage and co-expression with chaperone protein disulfide isomerase

(2013) Chong Sha et al.   ENZYME AND MICROBIAL TECHNOLOGY

Unconventional microbial systems for the cost-efficient production of high-quality protein therapeutics

(2012) José Luis Corchero et al.   BIOTECHNOLOGY ADVANCES

The Zinc Finger Proteins Mxr1p and Repressor of Phosphoenolpyruvate Carboxykinase (ROP) Have the Same DNA Binding Specificity but Regulate Methanol Metabolism Antagonistically inPichia pastoris

(2012) Nallani Vijay Kumar et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

ATP-dependent assembly of the heteromeric Pex1p–Pex6p-complex of the peroxisomal matrix protein import machinery

(2012) Delia Saffian et al.   JOURNAL OF STRUCTURAL BIOLOGY

Metabolic flux profiling of recombinant protein secreting Pichia pastoris growing on glucose:methanol mixtures

(2012) Joel Jordà et al.   Microbial Cell Factories

Pichia pastoris 14-3-3 regulates transcriptional activity of the methanol inducible transcription factor Mxr1 by direct interaction

(2012) Pabitra K. Parua et al.   MOLECULAR MICROBIOLOGY

Regulation of Pichia pastoris promoters and its consequences for protein production

(2012) Thomas Vogl et al.   New Biotechnology

Identification of Mxr1p-binding sites in the promoters of genes encoding dihydroxyacetone synthase and peroxin 8 of the methylotrophic yeast Pichia pastoris

(2010) Balla Venkata Kranthi et al.   YEAST

Identification of key DNA elements involved in promoter recognition by Mxr1p, a master regulator of methanol utilization pathway in Pichia pastoris

(2009) Balla Venkata Kranthi et al.   Biochimica et Biophysica Acta-Gene Regulatory Mechanisms

Genome, secretome and glucose transport highlight unique features of the protein production host Pichia pastoris

(2009) Diethard Mattanovich et al.   Microbial Cell Factories

Genome sequence of the recombinant protein production host Pichia pastoris

(2009) Kristof De Schutter et al.   NATURE BIOTECHNOLOGY

Engineering of bottlenecks in Rhizopus oryzae lipase production in Pichia pastoris using the nitrogen source-regulated FLD1 promoter

(2009) David Resina et al.   New Biotechnology

Strong negative self regulation of Prokaryotic transcription factors increases the intrinsic noise of protein expression

(2008) Dov J Stekel et al.   BMC Systems Biology

Hypoxic fed-batch cultivation ofPichia pastoris increases specific and volumetric productivity of recombinant proteins

(2007) Kristin Baumann et al.   BIOTECHNOLOGY AND BIOENGINEERING