Article
Cell Biology
Xing Guo, Zhenyu Zhang, Chaohui Lin, Huiling Ren, Yijing Li, Yuan Zhang, Yuxing Qu, Hongxin Li, Saiwen Ma, Huijuan Xia, Rongkuan Sun, Haoyu Zu, Yuezhi Lin, Xiaojun Wang
Summary: This study found that H1N1 pdm09 virus promotes its replication through autophagy, leading to faster spread. The results provide theoretical basis for analyzing the epidemiological characteristics of H1N1 pdm09 influenza and the development of anti-influenza drugs and vaccines.
Article
Cell Biology
Yuting Zhao, Zhongju Zou, Daxiao Sun, Yue Li, Sangita C. Sinha, Li Yu, Lynda Bennett, Beth Levine
Summary: GLIPR2 is a negative regulator of PtdIns3K-C1 activity and basal autophagy. Its depletion leads to increased autophagic flux and phosphatidylinositol 3-phosphate generation, with less compact Golgi structures observed.
Article
Cell Biology
Honglei Jin, Jiugao Ma, Jiheng Xu, Hongyan Li, Yuanyuan Chang, Nan Zang, Zhongxian Tian, Xin Wang, Nannan Zhao, Lu Liu, Caiyi Chen, Qipeng Xie, Yongyong Lu, Zhouxi Fang, Xing Huang, Chuanshu Huang, Haishan Huang
Summary: In this study, it was found that MIR516A is upregulated in human bladder cancer, acting as an oncogene by regulating PHLPP2 and inhibiting autophagy, thus promoting tumor growth. This novel oncogenic function of MIR516A in bladder cancer differs from its known tumor suppressor role in other cancers.
Editorial Material
Cell Biology
Yuchen Lei, Daniel J. Klionsky
Summary: VCP, a conserved ATPase, regulates autophagy initiation by stabilizing BECN1 and enhancing phosphati-dylinositol 3-kinase (PtdIns3K) complex assembly, in addition to its roles in autophagosome maturation.
Article
Biochemistry & Molecular Biology
Cuneyd Parlayan, Yunus Sahin, Zekiye Altan, Kaifee Arman, Masa-Aki Ikeda, Khandakar A. S. M. Saadat
Summary: The study focused on the role of ARID3A in osteosarcoma progression, revealing its impact on BECN1 expression and cell proliferation. Findings suggest that ARID3A may act as a tumor suppressor and influence the expression level of BECN1 in U-2 OS cells, shedding light on potential treatment strategies for osteosarcoma.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Immunology
Sergio Castro-Gonzalez, Sydney Simpson, Yuhang Shi, Yuexuan Chen, Jared Benjamin, Ruth Serra-Moreno
Summary: Ubiquitination plays a critical role in various aspects of HIV replication, influencing factors such as activity, localization, and stability. It also impacts the interactions between HIV and the cellular environment, specifically with regards to autophagy and apoptosis.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Cell Biology
Sergio Castro-Gonzalez, Yuhang Shi, Marta Colomer-Lluch, Ying Song, Kaitlyn Mowery, Sharilyn Almodovar, Anju Bansal, Frank Kirchhoff, Konstantin Sparrer, Chengyu Liang, Ruth Serra-Moreno
Summary: Macroautophagy/autophagy is an innate antiviral defense mechanism that targets virions and viral components for degradation, but its role in HIV infection is unclear. Studies show that autophagy poses a hurdle for HIV replication, but HIV-1 uses Nef to counteract this restriction.
Article
Reproductive Biology
Lu Ke, Xinyi Lin, Yuchuan Luo, Siming Tao, Chang Yan, Yifeilong He, Yingjie Wu, Ning Liu, Yinghe Qin
Summary: This study reveals the crucial role of core autophagy factor Becn1 in fertility and spermiogenesis in mice. Deficiency of Becn1 leads to reduced sperm quantity and motility, resulting in reduced fertility. Additionally, autophagy is found to play an important role in the process of spermatogenesis.
BIOLOGY OF REPRODUCTION
(2023)
Article
Oncology
Li Li, Yijia Gong, Ke Xu, Weihong Chen, Jiuyi Xia, Zhaobo Cheng, Lili Li, Renjie Yu, Junhao Mu, Xin Le, Qin Xiang, Weiyan Peng, Junying Tang, Tingxiu Xiang
Summary: This study evaluated the potential tumor suppressor gene ZBTB28 as a marker for cervical cancer assessment. The research found that ZBTB28 can induce autophagy-related apoptosis in cervical cancer cells, inhibiting tumor growth. Furthermore, ZBTB28 promoter methylation analysis may offer a new objective strategy for cervical cancer screening.
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
(2021)
Article
Cell Biology
Rupali Gund, Angela M. Christiano
Summary: Alopecia areata is a condition where the immune system attacks the hair follicle, leading to hair loss. Research suggests that impaired autophagy plays a role in the pathogenesis of this disease, with decreased autophagic activity in the hair follicles of affected mice.
Article
Cell Biology
Maria Naama, Shai Bel
Summary: Mucus secretion from colonic goblet cells is regulated by autophagy and endoplasmic reticulum stress. Pharmacological reduction of ER stress or activation of the UPR in mice leads to excess mucus secretion. This regulation is dependent on the gut microbiota and the intracellular sensor NOD2, and excess mucus production protects against inflammation.
Article
Cell Biology
Chengqian Li, Jun Xu, Qing Yu, Ping Wang, Bingzi Dong, Liyan Shen, Qing Wang, Shufa Li, Ying Yang, Yujie Deng
Summary: BECN1 plays a crucial role in adipocyte differentiation, lipolysis, and insulin resistance. Mutation at the acetylation site K414 of BECN1 affects the stability of the protein, influencing autophagy, differentiation, and lipolysis in adipocytes. These findings suggest that K414 of BECN1 may serve as a potential drug target for regulating autophagy and lipid metabolism in adipocytes.
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
(2021)
Article
Cell Biology
Xudong Xie, Liangcong Hu, Bobin Mi, Hang Xue, Yiqiang Hu, Adriana C. Panayi, Yori Endo, Lang Chen, Chenchen Yan, Ze Lin, Hui Li, Wu Zhou, Guohui Liu
Summary: This study demonstrated for the first time that metformin can alleviate postmenopausal bone loss by downregulating autophagy during osteoclastogenesis via E2F1-dependent regulation of BECN1 and BCL2.
CELL COMMUNICATION AND SIGNALING
(2022)
Article
Biochemistry & Molecular Biology
Shujuan Liu, Qiqi Bu, Jiashun Tong, Zhanhang Wang, Jiuzeng Cui, Heran Cao, Haidong Ma, Binyun Cao, Xiaopeng An, Yuxuan Song
Summary: This study revealed the differential expression of miR-486 in the ovaries of monotocous dairy goats and its significant effects on the survival, apoptosis, and autophagy of granulosa cells. Further experiments identified and characterized the interaction between miR-486 and SRSF3, demonstrating its regulatory role in granulosa cell function. These findings provide insights into the underlying molecular mechanism of miR-486 in ovarian follicle atresia and its target gene SRSF3.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Zehao Wang, Lu Yang, Peng Wu, Xing Li, Yuhui Tang, Xueqi Ou, Yue Zhang, Xiangsheng Xiao, Jin Wang, Hailin Tang
Summary: This study revealed that circROBO1 is upregulated in breast cancer liver metastasis and is associated with poor prognosis. CircROBO1 facilitates the carcinogenesis and liver metastasis of breast cancer through the circROBO1/KLF5/FUS feedback loop, which inhibits the selective autophagy of afadin by suppressing the transcription of BECN1.
Review
Biochemistry & Molecular Biology
Can Lin, Jihua Guo, Rong Jia
Summary: Treg-EVs, derived from regulatory T cells, serve as a novel cell-contact independent inhibitory mechanism by delivering specific biological molecules to target cells and modulating immune responses. They are associated with various human diseases and possess potential applications.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Di Li, Jihua Guo, Rong Jia
Summary: HNRNPK can partially epigenetically regulate cancer cell proliferation by increasing transcription and exon 4-inclusion of SPIN1. This study reveals a novel epigenetic regulatory pathway of HNRNPK in oral squamous cell carcinoma.
JOURNAL OF MOLECULAR BIOLOGY
(2023)
Review
Oncology
Miaomiao Liu, Jihua Guo, Rong Jia
Summary: Alternative RNA splicing is an important cellular process that, when dysregulated, contributes to cancer development. Dysregulated expression of splicing factors is involved in oral squamous cell carcinoma. Therapeutic approaches targeting RNA splicing are being developed.
FRONTIERS IN ONCOLOGY
(2022)
Article
Oncology
Lingyan Yan, Jiayun Wang, Jihua Guo, Rong Jia
Summary: The roles of enhancer RNAs (eRNAs) in gene expression regulation in human diseases have been increasingly recognized. However, little is known about the specific expression profile and function of eRNAs in oral squamous cell carcinoma (OSCC). This study aimed to investigate an uncharacterized eRNA, insulin receptor substrate 2 enhancer RNA (IRS2e), in OSCC. IRS2e was found to be overexpressed in OSCC and correlated with poor prognosis. Knockdown of IRS2e inhibited cell growth, induced apoptosis and cell cycle arrest in OSCC cells. Furthermore, IRS2e ablation inhibited tumor growth in vivo. Mechanistically, IRS2e was essential for the expression of nearby oncogene IRS2. Overall, IRS2e is a novel oncogenic eRNA required for IRS2 expression in OSCC.
Article
Biochemistry & Molecular Biology
Miaomiao Liu, Can Lin, Qiwei Huang, Jun Jia, Jihua Guo, Rong Jia
Summary: The inclusion level of Ki67 exon 7 is significantly associated with poor prognosis in multiple cancer types, and is essential for cell proliferation, cell cycle progression, cell migration, and tumorigenesis in head and neck squamous cell carcinoma (HNSCC). Additionally, it is positively correlated with intracellular reactive oxygen species (ROS) level. The study also identified a new regulatory axis involving SRSF3/Ki67/AKR1C2 during HNSCC tumor progression.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Lingyan Yan, Yanan Sun, Jihua Guo, Rong Jia
Summary: The lack of PD-L1 exon 3 leads to a significant loss of cellular membrane locations and reduced protein expression of PD-L1. Oral cancer cells do not skip exon 3 to ensure the expression of full-length, functional PD-L1 protein. Two key exonic splicing enhancers (ESEs) were discovered for exon 3 inclusion, and two efficient antisense oligonucleotides (ASOs) were identified to block these ESEs. Treatment with these ASOs enhances immune cells' ability to suppress cancer cell proliferation and also inhibits cell growth and induces cell pyroptosis in oral cancer cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Si-Rui Ma, Jian-Feng Liu, Rong Jia, Wei-Wei Deng, Jun Jia
Summary: ITGB4 plays an important role in mediating cell adhesion and has been implicated in tumorigenesis and metastasis. This study examined the expression of ITGB4 in oral squamous cell carcinoma (OSCC) and its relationship with other markers. The results showed that ITGB4 was significantly increased in OSCC, and a subgroup with high expression of ITGB4/PD-L1 and CD8/PD-1 had a better prognosis. These findings shed light on the potential role of ITGB4 in OSCC and provide a basis for further investigation.
Article
Dentistry, Oral Surgery & Medicine
Mi Xu, Bingrong Li, Junjun Huang, Rong Jia, Jihua Guo
Summary: In this study, the function and potential mechanism of Fto in dentine formation were investigated. Fto knockout in mice led to defects in dentine formation, with decreased mineralization ability and altered expression of odontoblastic mineralization related genes. Fto knockdown also affected the inclusion level of Runx2 exon 5 and destabilized RBM4 mRNA. These findings suggest that Fto plays a critical role in dentine formation.
INTERNATIONAL ENDODONTIC JOURNAL
(2023)
Article
Biochemistry & Molecular Biology
Can Lin, Miaomiao Liu, Jihua Guo, Rong Jia
Summary: Cryotherapy can effectively reduce inflammation in LPS-stimulated periodontal ligament cells by suppressing the lncRNA SNHG1/miR-9-5p/NFKB1 axis, according to transcriptome sequencing analysis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Rong Jia, Xiaoxuan Che, Jun Jia, Jihua Guo
Summary: The study revealed that FOXM1a reduces cell proliferation and tumor formation in oral squamous cell carcinoma, regulated by exon 9 splicing and hnRNP C. This discovery uncovers a novel regulatory mechanism of oncogene FOXM1 expression in OSCC.
Article
Biochemistry & Molecular Biology
Soojung Hahn, Gyuri Kim, Sang-Man Jin, Jae Hyeon Kim
Summary: This study utilized three-dimensional intestinal organoids to investigate the effects of metformin on inflammatory bowel disease (IBD) and found that metformin can enhance intestinal barrier function and reduce levels of inflammatory cytokines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
V. V. Sudarev, M. S. Gette, S. V. Bazhenov, O. M. Tilinova, E. V. Zinovev, I. V. Manukhov, A. I. Kuklin, Yu. L. Ryzhykau, A. V. Vlasov
Summary: This study investigated the self-assembly processes of ferritin-based protein complexes and obtained structurally characterized oligomeric states. These results provide new potential and opportunities for the application of ferritin in various fields.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Yalda Sabaghi, Farnaz Pourfarzad, Leila Zolghadr, Azita Bahrami, Tahereh Shojazadeh, Alireza Farasat, Nematollah Gheibi
Summary: p-Coumaric acid (p-CA) is a plant compound with anti-cancer activities. This study designed a nano-liposomal carrier containing p-CA to enhance its effectiveness against melanoma cells. The findings showed that the liposomal form of p-CA had a greater impact on the cells. Kinetic modeling indicated that the best fitting model was zero-order.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
M. D. Nazmul Hasan, Md Mahfuzur Rahman, Al Asmaul Husna, Nobuhiro Nozaki, Osamu Yamato, Naoki Miura
Summary: This study investigated the expression of ncRNAs other than miRNAs in different histologic subtypes of canine mammary gland tumors (MGT). Three aberrantly expressed ncRNAs were identified as potential biomarkers for differentiating MGT subtypes. YRNA and tRFs expression levels were found to be decreased in metastatic compared to primary MGT cell lines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Seine A. Shintani
Summary: In this study, the researchers used signal analysis to study the instantaneous amplitude and phase of sarcomeric oscillations in skeletal muscle. They identified two types of oscillations, sarcomeric oscillations and sarcosynced oscillations, and visualized their behavior during propagating waves. The researchers discovered the presence of sarcomeric defect holes and sarcomeric collision holes, which are important indicators for understanding the oscillation properties of sarcomeres. This finding has important implications for improving our understanding of muscle function and its regulatory mechanisms.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Shuanglin Zhang, Yuzhong Jia, Guolan Ma, Yanyan Yang, Zhenzhen Cao, Antao Luo, Zefu Zhang, Shihan Li, Jie Wen, Hanfeng Liu, Jihua Ma
Summary: Bupleurum is an antiarrhythmic agent that may exert its effects by inhibiting L-type calcium channels.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Tomotaka Ohkubo, Yasuhiko Matsumoto, Hiroaki Sasaki, Kaoru Kinoshita, Yuki Ogasawara, Takashi Sugita
Summary: This study found that Citrobacter koseri inhibits the growth of Staphylococcus epidermidis, disrupting the balance between S. epidermidis and Staphylococcus aureus, and exacerbating inflammation in atopic dermatitis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Toshifumi Asano, Philipp Sasse, Takao Nakata
Summary: A Cre recombination-based fluorescent reporter system was developed to monitor cell-cell fusion. The system successfully detected the formation of multinuclear myotubes and placental syncytiotrophoblast. This tool could facilitate the study of cell-to-cell fusion.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Ke Shi, Yunlong Shan, Xiao Sun, Kuida Chen, Qiong Luo, Qiang Xu
Summary: This study found that low expression of TP53INP2 is associated with poor survival in colorectal cancer (CRC) patients. As the malignancy of CRC progresses, TP53INP2 expression gradually decreases. Knockdown of TP53INP2 promotes CRC cell proliferation and tumor growth. Mechanistically, TP53INP2 deficiency decreases phosphorylation of beta-catenin, leading to increased accumulation and enhanced nuclear translocation and transcriptional activity. Additionally, TP53INP2 sequesters TIM50, inhibiting its activation of beta-catenin. In conclusion, downregulation of TP53INP2 promotes CRC progression by activating beta-catenin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Martina Rossi, Fabio Tomaselli, Alejandro Hochkoeppler
Summary: Oligomeric enzymes are known for their higher catalytic rates compared to monomeric enzymes, but the extent of additivity in their activity is still not well understood. This study used tetrameric rabbit lactate dehydrogenase as a model to examine the kinetics of its catalytic action. Surprisingly, when the concentration of the limiting reactant exceeded that of a single subunit, there was a significant slowdown in the enzyme's conformational rearrangements.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Amin Sahraei, Mohammad Javad Shamsoddini, Fakhrossadat Mohammadi, Leila Hassani
Summary: This study explored the inhibitory effects of gallium curcumin, indium curcumin, and vanadyl curcumin on the amyloid fibrillation of hen egg white lysozyme, as well as the binding interactions of these metal complexes with the enzyme. The results showed that indium curcumin and vanadyl curcumin exhibited higher binding affinities and stronger inhibitory effects on amyloid fibrillation compared to gallium curcumin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Takahiro Sasaki, Yoshiki Kuse, Shinsuke Nakamura, Masamitsu Shimazawa
Summary: PGRN deficiency plays a significant role in cardiac remodeling and arrhythmias post-myocardial infarction (MI), potentially by promoting metabolic abnormalities in macrophages.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Hongwei Zhao, Yiqiang Li, Yibo Zhang, Chi Zhang
Summary: Electrical brain stimulation technology is commonly used to treat brain neurological disorders, but it can cause side effects. This study investigated the impact of electric fields on nerve fibers and revealed the possible origin of side effects. The findings provide guidance for selecting electrical parameters in clinical stimulation therapy.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Julia S. Scott, Lake-Ee Quek, Andrew J. Hoy, Johannes V. Swinnen, Zeyad D. Nassar, Lisa M. Butler
Summary: The fatty acid elongation enzyme ELOVL5 plays a critical role in promoting metastasis in prostate cancer. Knocking down ELOVL5 leads to the accumulation of malonyl-CoA, which inhibits fatty acid oxidation in mitochondria. This study highlights the importance of fatty acid elongation in regulating cell viability and provides a potential target for prostate cancer treatment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Zan Zhou, Wen-jun Jiang, Li Li, Jun-qiang Si
Summary: This study investigates the effect of noise exposure on cognitive function in mice and explores the underlying molecular mechanisms. The findings suggest that noise exposure leads to increased inflammation, increased phosphorylation of Tau protein, and decreased levels of postsynaptic density protein, resulting in cognitive impairment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
