Article
Chemistry, Applied
Yingying Zhang, Mengyao Liu, Shouwei Wang, Chaodi Kang, Mingyue Zhang, Yingying Li
Summary: Food adulteration is a global problem that threatens public health safety and the healthy development of the food industry. This study established an LC-MS/MS method for accurate identification and quantitative analysis of fox meat products, successfully screening fox-specific peptides for precise quantification.
Article
Biochemical Research Methods
Xinxin Ye, Bin Zhu, Yang Chen, Yingfeng Wang, Dan Wang, Zhigang Zhao, Zhongfeng Li
Summary: This study performed integrated metabolomics and lipidomics analysis on healthy subjects and cerebral infarction patients, identifying metabolite and lipid biomarkers and enriching disturbed pathways. The results suggest that the approach could contribute to understanding the metabolic dysregulation associated with cerebral infarction pathogenesis and may aid in its early diagnosis.
JOURNAL OF PROTEOME RESEARCH
(2022)
Article
Biochemical Research Methods
Xinxin Ye, Bin Zhu, Yang Chen, Yingfeng Wang, Dan Wang, Zhigang Zhao, Zhongfeng Li
Summary: This study integrated metabolomics and lipidomics analysis to identify 49 metabolite and 68 lipid biomarkers associated with the pathogenesis of cerebral infarction. A support vector machine model constructed using these biomarkers showed excellent predictive ability for early diagnosis.
JOURNAL OF PROTEOME RESEARCH
(2022)
Article
Biochemical Research Methods
Yifan Xu, Haonan Li, Yiqun Han, Teng Wang, Yanwen Wang, Jicheng Gong, Ke Gao, Wu Chen, Weiju Li, Hongyin Zhang, Junxia Wang, Xinghua Qiu, Tong Zhu
Summary: Sphingolipids are a class of lipids with diverse structures and biological functions, and have been proposed as potential biomarkers for cardiometabolic diseases. The current methods for quantifying sphingolipids have limitations in terms of sample pretreatment and volume. In this study, a simple and rapid method using methanol for plasma sphingolipid extraction was developed, and 37 sphingolipids from 8 classes were successfully quantified using liquid chromatography coupled with tandem mass spectrometry. This method showed good performance and was applied successfully in an epidemiological study.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Yuqi Lin, Xugui Li, Mengxiang Dai, Qiaoyu Li, Qingxin Shi, Lijun Zhang, Rongzeng Huang, Chengwu Song, Shuna Jin
Summary: This research identified and compared the distribution and sex differences of cardiolipins (CLs) in heart, liver, kidney, spleen, lung, skeletal muscle, and brain using ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UHPLC-QTOF-MS/MS). The results showed significant differences in the distribution of CLs in spleen, lung, and brain compared to heart, liver, kidney, and skeletal muscle. Sex differences of CLs in liver and kidney were also observed. These findings provide evidence for the dynamic states and sex differences of CLs, and shed light on the mitochondrial-related mechanism under physiological and pathological conditions.
Article
Clinical Neurology
Meiling Huang, Shaohang Xu, Mingchao Zhou, Jiao Luo, Fubing Zha, Linlin Shan, Qingqing Yang, Baojin Zhou, Yulong Wang
Summary: This study conducted lipidomic analyses on plasma metabolic features in stroke patients and healthy individuals, identifying potential lipid markers related to long-term functional recovery in stroke patients. The results suggest that lysophosphatidylcholines (LPC) may play a positive role in patient rehabilitation through an anti-inflammatory mechanism. Appropriate management or intervention for lipid levels is expected to lead to better clinical outcomes.
FRONTIERS IN NEUROLOGY
(2022)
Article
Food Science & Technology
Run Zhang, Man Yang, Xinhua Hou, Renda Hou, Ligang Wang, Lijun Shi, Fuping Zhao, Xin Liu, Qingshi Meng, Lixian Wang, Longchao Zhang
Summary: This study investigated the characterization and difference of lipids and metabolites between JWX and LW pork using metabolomic and lipidomic profiling. The results showed that JWX pork had higher intramuscular fat content and meat redness compared to LW pork. The composition of metabolites and lipids also showed significant differences between the two types of pork.
FOOD RESEARCH INTERNATIONAL
(2022)
Article
Biochemical Research Methods
Kacey D. Cliburn, Marilyn A. Huestis, Jarrad R. Wagner, Philip M. Kemp
Summary: A liquid chromatography-tandem mass spectrometry method was developed and validated for quantifying cannabinoids in postmortem specimens, showing accuracy and stability in measuring various cannabinoids.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Chemistry, Analytical
Fahad Ahmed, Benjamin Tscharke, Jake W. O'Brien, Jack Thompson, Qiuda Zheng, Jochen F. Mueller, Kevin Thomas
Summary: Wastewater-based epidemiology is a useful tool for analyzing patterns of community drug use, with a method developed and validated to track analgesics in wastewater. By using liquid chromatography coupled to tandem mass spectrometry, the method can detect analgesics in wastewater to estimate consumption levels.
Article
Chemistry, Analytical
A-Yeong Ko, Jun-Young Yang, Donghwi Kim, Han Young Eom, Jong-Hwa Lee
Summary: This study compared and validated methods using surrogate matrix and surrogate analytes to quantify endogenous T-3 and T-4 in rat serum. The results indicate that the surrogate matrix method is more accurate and cost-effective.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2021)
Article
Biochemical Research Methods
Mathias Langner, Ivona Mateska, Nicole Bechmann, Ben Wielockx, Triantafyllos Chavakis, Vasileia Ismini Alexaki, Mirko Peitzsch
Summary: In this study, a simple LC-MS/MS method is described for accurately quantifying arginine and its related metabolites in different experimental samples. The method is suitable for various applications and shows good performance in quantifying arginine metabolites in cell lysates, cell culture supernatants, and tissue extracts.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Review
Spectroscopy
Mona M. Khamis, Darryl J. Adamko, Anas El-Aneed
Summary: Metabolomics is an important field with challenges in biomarker qualification for clinical applications, due to issues such as inappropriate study designs and insufficient data to support clinical utility. To address these challenges, modifications of existing guidelines and alternative approaches are being developed to facilitate method validation. The main challenges in method development and validation for endogenous metabolites include matrix effects evaluation, alternative analyte-free matrices, and the choice of internal standards.
MASS SPECTROMETRY REVIEWS
(2021)
Article
Biochemical Research Methods
Yuanyuan Lu, Zhitong Mai, Hongxia Zhou, Wenda Guan, Shiguan Wu, Heyan Zou, Maoting Shen, Yangqing Zhan, Feng Ye, Minshan Qiu, Lihan Shen, Beibei Zhao, Zifeng Yang
Summary: This study developed a reliable and sensitive method for quantifying eicosanoids in human serum. The method showed good linearity, high recovery rates, and low limits of quantification. In clinical application, different characteristic metabolite profiles were found in patients with severe pneumonia.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Yan-Qing Chen, Hong Shen, Ru-Jie Yang, Jian-Bo Wan
Summary: A sensitive method was developed to accurately determine 20 NAEs in biological samples by chemical isotope labeling strategy coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS). Derivatized NAEs provided stable and abundant specific product ions in MS/MS spectrum for quantitation, enhancing detection sensitivity by 6- to 170-fold compared to non-derivatized NAEs. The method was successfully applied to determine NAEs in serum samples and liver tissues, showing practicability in analyzing endogenous NAE in biological samples.
ANALYTICA CHIMICA ACTA
(2021)
Article
Biotechnology & Applied Microbiology
Gaela Cauchie, Guillaume Delfau-Bonnet, Guillaume Caulier, Anne-Lise Hantson, Jean-Hugues Renault, Pascal Gerbaux
Summary: Liquid chromatography - mass spectrometry (LC-MS) experiments were used to monitor the impact of bead milling and extraction solvents on the composition of lipids extracted from Microchloropsis gaditana microalgae. It was found that milling before extraction can enhance the efficiency of lipid extraction with n-heptane as the solvent. The study also highlighted the importance of liquid chromatography tandem mass spectrometry (LC-MS/MS) experiments in optimizing lipid extraction procedures from microalgae biomass.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2021)
Article
Pharmacology & Pharmacy
Huarong Xu, Jiameng Qu, Jian Wang, Kefei Han, Qing Li, Wenchuan Bi, Ran Liu
Summary: This study developed a new method using high resolution mass spectrometry and in silico strategy to search for potential chemical ingredients in Polygonum cuspidatum (PC) that target the TGF-I3 RI. Resveratrol, a compound found in PC, was identified to have excellent biological activity on TGF-I3 RI and potential as a treatment for PF.
JOURNAL OF PHARMACEUTICAL ANALYSIS
(2022)
Article
Biochemistry & Molecular Biology
Kunqian Mu, Kaiwen Jiang, Yue Wang, Zihan Zhao, Song Cang, Kaishun Bi, Qing Li, Ran Liu
Summary: A LC-MS/MS method was developed to study the in vivo fate of beta-cyclodextrin. The results showed that beta-cyclodextrin has a wide distribution in rats' bodies and is rapidly excreted, indicating low accumulation and good safety.
Article
Biochemistry & Molecular Biology
Ziru Liu, Jiameng Qu, Fan Ke, Haotian Zhang, Yiwen Zhang, Qian Zhang, Qing Li, Kaishun Bi, Huarong Xu
Summary: This study elucidated the antioxidant material basis of dandelion through spectrum-effect relationship analysis and provided a scientific basis for the quality control of dandelion.
Article
Plant Sciences
Jiameng Qu, Fan Ke, Ziru Liu, Xiao Yang, Xianzhe Li, Huarong Xu, Qing Li, Kaishun Bi
Summary: This study comprehensively demonstrates the multi-target mechanisms of dandelion against TNBC using network pharmacology, molecular pharmacology, and metabolomics approaches. The findings indicate that dandelion can treat TNBC by regulating cell cycle and metabolism-related biological processes. These findings are of great importance for further mechanism investigations and the development of highly effective dandelion-based treatments for TNBC.
Article
Medicine, Research & Experimental
Xiaomeng Sun, Lun Ai, Yinfei Ran, Yiwen Zhang, Qian Zhang, Qing Li, Yan Cui, Lixin Sun
Summary: In this study, the representative components of Sang Xing decoction (SXD) were characterized using liquid chromatography-tandem mass spectrometry (LC-MS). The key targets, signaling pathways, and metabolic pathways associated with SXD in the treatment of acute bronchitis were identified via network prediction and metabolomics. In vivo experiments showed that SXD may maintain the production of inflammatory factors by regulating the PI3K/Akt/NF-kappa B signaling pathway, improving the metabolism of glutamine and glutamate to reduce oxidative stress, and inhibiting apoptosis. Simultaneously, the possibility of using SXD as an adjuvant drug for COVID-19 treatment was also revealed.
BIOMEDICINE & PHARMACOTHERAPY
(2022)
Article
Chemistry, Analytical
Song Cang, Ran Liu, Kunqian Mu, Qi Tang, Haiyue Cui, Kaishun Bi, Yiwen Zhang, Qing Li
Summary: This study investigated the pathological changes of non-small cell lung cancer (NSCLC) from the perspective of endogenous polar and nonpolar molecules. Targeted metabolomics and lipidomics studies were conducted, revealing potential small molecule and lipid biomarkers for NSCLC. A new strategy for formulating an equation for NSCLC identification was proposed and successfully evaluated through clinical utility.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2022)
Article
Plant Sciences
Yiwen Zhang, Saiyu Li, Panpan Yang, Qian Zhang, Huarong Xu, Kaishun Bi, Qing Li
Summary: This study established a novel and multidimensional quality assessment approach for Traditional Chinese Medicines (TCMs) using Schisandra chinensis as an example, identifying comprehensive quality markers based on pharmacodynamics and chemical properties.
Article
Chemistry, Analytical
Chunyu Yu, Qian Zhang, Liyuan Wang, Yiwen Zhang, Meiyu Cui, Kaishun Bi, Donghao Li, Qing Li
Summary: A nanoconfined liquid phase nanoextraction (NLPNE) combined with in-fiber derivatization (IFD) strategy was proposed for simultaneous quantification of seventy amino-containing analytes in plasma. This method offers advantages of broad coverage, high sensitivity, time-and solvent-saving, and is applicable for cancer diagnosis.
Article
Food Science & Technology
Bo-sai He, Xiao Wang, Ying Zhang, Cheng Gao, Cheng-kai Wu, Si-rui Guo, Yan-ting Gu, Qing Li, Jia-hong Wang
Summary: The seeds of Moringa oleifera Lam have renal protective effects in diabetic nephropathy by modulating multiple signaling pathways, exerting anti-inflammatory, anti-fibrosis, and anti-oxidative effects.
JOURNAL OF FUNCTIONAL FOODS
(2022)
Article
Oncology
Meilin Qu, Tao Han, Xiaoquan Chen, Qingqing Sun, Qing Li, Mingfang Zhao
Summary: This study discovered that Actinidia chinensis Planch root can regulate the immune response of hepatocellular carcinoma (HCC) by targeting EGF, BIRC5, and SPP1. A novel immune-based gene signature model was developed to predict the survival of HCC patients.
JOURNAL OF GASTROINTESTINAL ONCOLOGY
(2022)
Article
Biochemical Research Methods
Minghui Tong, Qian Zhang, Yiwen Zhang, Luwen Xing, Kaishun Bi, Qing Li
Summary: Recent studies have shown that dysregulation of carboxyl submetabolome homeostasis is closely related to the occurrence and development of hepatocellular carcinoma (HCC). This study established a convenient and efficient workflow for absolute quantification of carboxyl submetabolome, which was applied to monitor carboxyl metabolic profile in human plasma of the whole progressive course of HCC. Biomarkers of health-hepatitis-cirrhosis-HCC were screened out and a diagnostic model was established. The relevancy between carboxyl submetabolome disorders and the pathogenesis of HCC was also investigated.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Plant Sciences
Jiameng Qu, Fan Ke, Xiao Yang, Yue Wang, Huarong Xu, Qing Li, Kaishun Bi
Summary: The study found that dandelion can alleviate DOX-induced cardiotoxicity but attenuate the cytotoxicity of DOX towards TNBC by activating the drug efflux transporter P-glycoprotein.
Article
Chemistry, Analytical
Qian Zhang, Minghui Tong, Huarong Xu, Yidi Yin, Peng Gao, Kaishun Bi, Yiwen Zhang, Qing Li
Summary: The lack of adequate lipid reference standards is a major challenge for accurate quantitative analysis in lipidomics. Through the discovery of regularity and predictability in chromatography-mass spectrometry behaviors of lipids, a protocol and strategy were developed to calculate correlations between compound structures and analytical parameters within each lipid subclass. This allowed for the prediction of relevant LC-tandem-MS parameters for unknown lipids, and the establishment and validation of a quantification parameter database for 4081 lipids. This methodology was applied to monitor lipid metabolism changes in the plasma of health-hepatitis-cirrhosis-hepatocellular carcinoma (HCC) progression, and resulted in the identification of lipid biomarkers for distinguishing between health and different diseases, as well as different stages of disease.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2023)
Article
Biochemistry & Molecular Biology
Luwen Xing, Yiwen Zhang, Saiyu Li, Minghui Tong, Kaishun Bi, Qian Zhang, Qing Li
Summary: Hepatocellular carcinoma (HCC) is the terminal stage of chronic liver diseases, and chronic inflammation may contribute to its formation. Dysregulation of bile acid homeostasis in the enterohepatic circulation is a key factor in the development of HCC. This study used a rat model to reproduce HCC and monitored the bile acid profile in plasma, liver, and intestine. Differences in bile acid levels, including potential biomarkers for early diagnosis of HCC, were identified. The study also identified a key enzyme, bile acid-CoA:amino acid N-acyltransferase (BAAT), involved in bile acid synthesis and the inflammatory-cancer transformation process. This research provides insights into the role of bile acid metabolism in the liver-gut axis during the inflammation-cancer transformation process and has implications for HCC diagnosis, prevention, and treatment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Qi Tang, Ran Liu, Ge Chu, Yue Wang, Haiyue Cui, Tongrui Zhang, Kaishun Bi, Peng Gao, Zonghua Song, Qing Li
Summary: The lungs and large intestine can regulate inflammation and immunity through the lung-gut axis. The transportation of gut microbiota and metabolites is an important communication channel. During the transition from ulcerative colitis (UC) to colorectal cancer (CRC), the composition of gut microbiota and metabolites related to inflammation changes significantly, and corresponding inflammatory changes also occur in lung tissues. By detecting the changes in pulmonary metabolites and microbiota, key markers related to lung and gut diseases can be identified.