Article
Chemistry, Analytical
Kosar Shahsavar, Ehsan Shokri, Morteza Hosseini
Summary: A new colorimetric platform based on spherical nucleic acid (SNA) and G-quadruplex DNAzymes has been developed for rapid and sensitive detection of specific miRNAs. The platform utilizes hybridization between target miRNA and capture probes to achieve detection. The method is capable of detecting miRNA-155 at concentrations as low as 0.7 nM and can be applied to real human serum samples.
Article
Nanoscience & Nanotechnology
Peng Shen, Qinyu Ge, Yihan Zhao, Mingsong Zhu, Ting Huang, Qingjiang Sun
Summary: This study reports the construction of a core-shell-nanostructured dual-emission quantum dot-loaded cerasome spherical nucleic acid that acts as an intracellular nanobiosensor. The nanobiosensor uses AND gate-guided dual-DNAzyme walker-mediated resonance energy transfer to detect dual endogenous markers and differentiate cancer/normal cells and cancer cell subtypes with improved imaging contrast and accuracy.
ACS APPLIED NANO MATERIALS
(2022)
Article
Chemistry, Analytical
Xinyi Zhao, Hai Wang, Yinghui Feng, Hexiang Li, Qi Liu, Xiaoqing Chen
Summary: This study presents a ratiometric amplifier based on quantum dots and spherical nucleic acids for accurate, efficient, and sensitive detection and imaging of miRNAs inside living cells, showing stability and recognition capability. The amplifier demonstrates high efficiency and excellent detection performance in vitro.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Biochemistry & Molecular Biology
Tomo Takegawa-Araki, Kai Yasukawa, Norihiko Iwazaki, Masayuki Utsugi, Hiroyuki Furukawa, Shinji Kumagai, Hiroaki Sawamoto, Satoshi Obika
Summary: -Amino-LNA can acquire various functions through chemical modification at the 2 '-nitrogen atom. This study focused on the practical usefulness of 2 '-N-alkylaminocarbonyl 2 '-amino-LNA as a chemical modification of anti-miRNA oligonucleotide. The synthesis of 2 '-N-alkylaminocarbonyl substituted 2 '-amino-LNA monomers improved the duplex stability for complementary RNA strands and nuclease resistance, and increased the potency of anti-miRNA oligonucleotides.
BIOORGANIC & MEDICINAL CHEMISTRY
(2023)
Article
Biophysics
Yi Li, Mei-Ling Liu, Wen-Bin Liang, Ying Zhuo, Xiao-Jing He
Summary: This study elaborated a universal approach to quickly activate CRISPR/Cas12a for low-abundance biomarker detection based on the amplification strategy of a target-induced spherical nucleic acid enzyme (SNAzyme) network. The prepared biosensor exhibited satisfying detection performance for the biomarker with a wide linear range and a low detection limit, showing significant potential in clinical diagnoses.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Chemistry, Multidisciplinary
Mohamad Zandieh, Kshiti Patel, Juewen Liu
Summary: Microplastic pollution can pose a threat to human health through water and food chains. This study investigated the adsorption of DNA oligonucleotides on different microplastics and examined the impact of environmentally abundant metal ions. The results showed that PET and PS had the highest affinity for linear DNA, and the presence of Ca2+ and Mg2+ enhanced the adsorption of DNA.
Review
Chemistry, Inorganic & Nuclear
Qian Zhang, Cong Su, Jian-Ge Qiu, Bing-Hua Jiang, Chun-yang Zhang
Summary: To maintain the stability and integrity of cellular genomic DNA, DNA repair enzymes play a crucial role in repairing DNA damage caused by various factors. Abnormal DNA repair enzyme activity can lead to cell death, cancer, and other human diseases. To accurately monitor DNA repair enzymes in cells, enzyme-free nucleic acid amplification approaches have been introduced for real-time imaging. These approaches provide new insights into the dynamic changes of DNA repair enzymes at the single-cell level.
COORDINATION CHEMISTRY REVIEWS
(2023)
Article
Biochemistry & Molecular Biology
Liming Wang, Kern Hast, Tushar Aggarwal, Melih Baci, Jonathan Hong, Enver Cagri Izgu
Summary: This study presents a rapid method for developing aptamer-based fluorescent biosensors that can specifically detect miRNAs. The method is applicable to complex biological environments and requires minimal sample handling.
BIOORGANIC & MEDICINAL CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Christian Wiraja, Yutaro Mori, Takaharu Ichimura, Jangsun Hwang, Chenjie Xu, Joseph Bonventre
Summary: This study developed a human kidney tubuloid system combined with NF mRNA nanosensors for real-time evaluation of drug-induced nephrotoxicity. The research showed that NF nanosensors are specific and efficient in assessing tubular cell injury.
Article
Biophysics
Jiahao Li, Xuping Wu, Yue Li, Xin Wang, Huachuan Huang, Dan Jian, Yanke Shan, Yue Zhang, Chengcheng Wu, Guolei Tan, Shouyu Wang, Fei Liu
Summary: This smartphone-based HBV detecting technique offers a cost-effective, portable and user-friendly solution for HBV detection in underdeveloped areas. It has high sensitivity and accuracy, quantifies HBV viral load quickly, and has the potential for mobile HBV diagnoses and sharing epidemic information in resource-limited situations.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Chemistry, Analytical
Koji Karasawa, Hidetoshi Arakawa
Summary: This article introduces a miRNA analysis system that combines nucleic acid sequence-based amplification (NASBA) and chemiluminescence methods, which can be used for point-of-care testing. This method is compact, simple, rapid, and highly sensitive, and it is of great significance for the development of future point-of-care testing systems.
Article
Chemistry, Analytical
Fang Liu, Ru Chen, Wenlu Song, Liangwen Li, Chunyang Lei, Zhou Nie
Summary: This study proposed a colorimetric protease assay by combining proteolysis and RNA synthesis to trigger SNAs aggregation, achieving sensitive and specific detection of MMP-2. The method's applicability can be extended to other protease biomarkers and can enable portable detection with smartphone, highlighting its potential for point-of-care diagnosis of diseases.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Miguel Xavier, Maria-Eleni Kyriazi, Stuart Lanham, Konstantina Alexaki, Elloise Matthews, Afaf H. El-Sagheer, Tom Brown, Antonios G. Kanaras, Richard O. C. Oreffo
Summary: Researchers have developed DNA-gold nanoparticles to identify and sort SSCs with specific mRNA signatures, significantly enriching these skeletal stem cells. This method has the potential to enhance our understanding of bone cell biology and translational applications.
Article
Chemistry, Analytical
Yukiko Kamiya, Hiroshi Kamimoto, Hongyu Zhu, Hiroyuki Asanuma
Summary: miRNAs are small non-coding RNAs that regulate gene expression through the RNAi system in cells. Different steps regulate the generation of miRNA, and using FRET-labeled pre-miRNAs can help understand the maturation process of miRNA.
Article
Chemistry, Multidisciplinary
Minjie Lin, Yuanyuan Chen, Sisi Zhao, Rui Tang, Zhou Nie, Hang Xing
Summary: By utilizing liposome fusion-based transport, functional DNA can be applied to both external and internal cell surfaces, expanding the applications of cell engineering. This approach allows for efficient and spatially controlled engineering of cell membranes, enabling the construction of anisotropic membrane structures.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Chemistry, Multidisciplinary
Jiao Zheng, Qiwei Wang, Lin Shi, Pai Peng, Lili Shi, Tao Li
Summary: The study introduces a strategy for real-time monitoring ATP exocytosis and drug-stimulated apoptosis in cancer cells by constructing a DNA logic-gated nanoplatform. This platform captures endogenous ATP released during chemotherapy drug stimulation, providing a sensitive approach for dynamically evaluating drug-induced apoptosis and therapeutic efficacy.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Chemistry, Analytical
Yudie Sun, La Fang, Yunxiang Han, Aobo Feng, Shengjun Liu, Kui Zhang, Jing-Juan Xu
Summary: Myocardial miRNAs in peripheral blood play a crucial role in the pathogenic process of myocardial infarction. Researchers have developed a ratiometric electrochemiluminescence biosensor for the rapid identification and accurate quantification of myocardial miRNAs, which has significant implications in clinical interventions for treating cardiovascular lesions.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Ling Li, Huan Li, Lin Shi, Lili Shi, Tao Li
Summary: The research on antioxidant biomimetic enzymes has gained attention due to their potential in combating oxidative stress-related diseases. In this study, Sn-metallized porphyrins were found to be effective SOD mimics, exhibiting high catalytic activity and stability. The Sn-porphyrin-based nanozymes could be a promising alternative to natural SODs in clinical treatments.
Article
Chemistry, Analytical
Jiao Zheng, Qiwei Wang, Lin Shi, Lili Shi, Tao Li
Summary: This study presents a novel target delivery system that utilizes the 2D-to-3D transformation of nucleic acid nano vehicles to enhance cellular internalization and enable live-cell mRNA imaging. This system holds potential for precise disease treatment.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Yi Du, Qiwei Wang, Lili Shi, Tao Li
Summary: DNA-assembled multiaptamer systems offer a promising approach for targeted delivery of nucleic acid cargoes. In this study, a G-quadruplex-proximized aptamer system was engineered for efficient transmembrane delivery of siRNA, showing great potential for precise anticancer therapy.
Article
Chemistry, Analytical
Lin Shi, Chengbin Liu, Han Wang, Jiao Zheng, Qiwei Wang, Lili Shi, Tao Li
Summary: This study presents a fast and ultrasensitive miRNA detection method using a direct electrochemiluminescence sensing strategy, which avoids time-consuming signal amplification steps. The method enables the detection of AMI-related miRNA in human serum within 30 minutes and is of great significance for clinical AMI diagnosis.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Yang Yi, Yunxiang Han, Xi Cheng, Zhe Zhang, Yudie Sun, Kui Zhang, Jing-Juan Xu
Summary: The rapid detection of MRSA using the Exo III-assisted nanomotor mower for building 3D hotspots through SERS shows high sensitivity and wide linear range, with potential applications in food detection, water detection, clinical treatment, and diagnosis.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Shiyang Qu, Lin Shi, Huan Li, Tao Li
Summary: The research team used silicon nanoparticles to modify gold nanoparticles as label-free SNAzymes for nucleic acid detection. They found that SiO2 cores can efficiently adsorb various G4 sequences and successfully constructed a highly sensitive chemiluminescence nanoprobe. Through this method, they achieved ultrahigh sensitive chemiluminescence detection of AMI-related nucleic acids.
SENSORS & DIAGNOSTICS
(2023)
Article
Chemistry, Analytical
Lin Shi, Haiying Cai, Han Wang, Qiwei Wang, Lili Shi, Tao Li
Summary: In this study, a novel method based on the FNA nanoplatform is proposed for the effective capture of exosomes and direct detection of internal miRNAs. The results show that miR-21 has a negative regulatory role in apoptosis.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Huan Li, Qiwei Wang, Lili Shi, Tao Li
Summary: This study developed a fluorescent probe based on tetraazacycle for sensitive detection of endogenous Hcy in AMI patients' serum and cardiomyocytes. The levels of Hcy in human circulating blood were statistically analyzed, showing a significant difference between AMI patients and healthy participants. Comparison of Hcy expression levels in cardiomyocytes and other tissue cells revealed a lower level of endogenous Hcy in cardiomyocytes.
Article
Chemistry, Analytical
Jie Xu, Lin Shi, Han Wang, Tao Li
Summary: This study reports the design of a CRET-based multicolor sensing nanoplatform with a portable smartphone as mini-detector for simultaneous and sensitive analysis of multiple circulating miRNAs. By constructing MNAzyme@AuNP nanoprobes and producing high catalytic activity SNAzyme, a multicolor sensing platform for visualizing multiple miRNAs was successfully achieved with high sensitivity and selectivity in detecting AMI-related biomarkers, miR-133a and miR-499, in human serum.
ANALYSIS & SENSING
(2021)
