Article
Pathology
Moritz Jesinghaus, Melanie Boxberg, Maxime Schmitt, Mark Kriegsmann, Alexander Harms, Corinna Lang, Thomas Muley, Hauke Winter, Katharina Kriegsmann, Arne Warth, Albrecht Stenzinger, Carsten Denkert, Hans Hoffmann, Seyer Safi, Wilko Weichert
Summary: Cellular dissociation grading (CDG) based on tumor budding and cell nest size can be used on biopsies of pulmonary squamous cell carcinoma (pSCC) and has a significant impact on disease prognosis.
JOURNAL OF PATHOLOGY CLINICAL RESEARCH
(2022)
Article
Oncology
Hayel Derani, Anne-Sophie Becker, Oliver Hakenberg, Andreas Erbersdobler
Summary: This study examined the use of Cellular Dissociation Grade (CDG) in penile squamous cell carcinoma and verified its correlation with the pathologic status of regional lymph nodes. The results showed a significant association between CDG and prognosis in squamous cell carcinoma.
Article
Dentistry, Oral Surgery & Medicine
Luiz Arthur Barbosa da Silva, Maria Luiza Diniz de Sousa Lopes, Melka Coelho Sa, Roseana de Almeida Freitas, Ricardo Della Coletta, Ericka Janine Dantas da Silveira, Marcia Cristina da Costa Miguel
Summary: This study compared the prognostic significance of four histopathologic grading systems in 80 cases of oral tongue squamous cell carcinoma (OTSCC). The results indicated that the BD model was the only histopathologic grading system associated with the outcome of patients with OTSCC.
JOURNAL OF ORAL PATHOLOGY & MEDICINE
(2021)
Article
Cell Biology
Zhi-Wen Li, Lu He, Zhong Zheng, Qian Zhang, Yu-Ting Xu, Jie-Yu Chen, Jiong Shi, Wen-Bin Huang, Xiang-Shan Fan
Summary: This study introduced a novel Combined Grading system based on CNS and DR in ESCC, which demonstrated superior prognostic prediction compared to Cellular Dissociation Grading.
Article
Oncology
Zhuo Li, Linxiu Liu, Bingzhi Wang, Jianming Ying, Jie He, Liyan Xue
Summary: This study aimed to identify the standard of tumor-infiltrating lymphocyte (TIL) and tumor budding (TB) evaluations and build a predictive model. The experiment showed that TB assessment using immunohistochemistry staining and a 20x objective lens can effectively predict lymph node metastasis and survival. It was also found that low levels of pTIL were significant indicators of lymph node metastasis and unfavorable prognosis.
Article
Pathology
Cigdem Ozturk, Husniye Esra Pasaoglu, Funda Emre, Tulin Senturk Ege, Umit Seza Tetikkurt
Summary: Tumor budding is significantly correlated with the survival time of patients, while the new grading system is not associated with cell nest size and life expectancy.
INDIAN JOURNAL OF PATHOLOGY AND MICROBIOLOGY
(2022)
Article
Surgery
Andrea Beer, Annabell Reber, Matthias Paireder, Sebastian F. Schoppmann, Stefan Heber, Ana-Iris Schiefer
Summary: In pretherapeutic biopsies of esophageal and gastroesophageal junction tumors, the number of budding foci can predict overall survival independently, especially for grade 2 tumors.
Article
Gastroenterology & Hepatology
Hiroyuki Terasawa, Hideaki Kinugasa, Kazuhiro Nouso, Shumpei Yamamoto, Mami Hirai, Takehiro Tanaka, Akinobu Takaki, Hiroyuki Okada
Summary: Our study clarified that tumor volume and invasion are closely related to the detection of ctDNA, and when the tumor is completely resected, ctDNA is no longer detected after one or more days.
WORLD JOURNAL OF GASTROENTEROLOGY
(2021)
Article
Oncology
Hong Wu, Xuefeng Leng, Qianshi Liu, Tianqin Mao, Tao Jiang, Yiqiang Liu, Feifei Li, Chenhui Cao, Jun Fan, Liang Chen, Yaqi Chen, Quan Yao, Shun Lu, Renchuan Liang, Lanlin Hu, Mingxin Liu, Yejian Wan, Zhaoshen Li, Jun Peng, Qiyu Luo, Hang Zhou, Jun Yin, Ke Xu, Mei Lan, Xinhao Peng, Haitao Lan, Gang Li, Yongtao Han, Xia Zhang, Zhi-Xiong Jim Xiao, Jinyi Lang, Guihua Wang, Chuan Xu
Summary: Analysis of intratumoral microbiota in patients with esophageal cancer reveals a microbiota signature that is associated with chemoimmunotherapy response, showing that Streptococcus stimulates CD8+ T-cell infiltration and induces a favorable response. These findings suggest the potential clinical utility of intratumoral microbiota for cancer immunotherapy.
Article
Multidisciplinary Sciences
Xiannian Zhang, Linna Peng, Yingying Luo, Shaosen Zhang, Yang Pu, Yamei Chen, Wenjia Guo, Jiacheng Yao, Mingming Shao, Wenyi Fan, Qionghua Cui, Yiyi Xi, Yanxia Sun, Xiangjie Niu, Xuan Zhao, Liping Chen, Yuqian Wang, Yachen Liu, Xinyu Yang, Chengcheng Wang, Ce Zhong, Wen Tan, Jianbin Wang, Chen Wu, Dongxin Lin
Summary: The study analyzed 60 ESCC patient samples using scRNA-seq, TCR-seq and genomics to identify prognostic markers associated with survival and immunosuppressive microenvironments. It revealed the complex tumor ecosystem of ESCC and the interactions between cancer cells and other cells in the tumor microenvironment. The study identified several markers significantly associated with patients' survival, providing insights for precision care of ESCC patients.
NATURE COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Dariush Nasrollahzadeh, Gholamreza Roshandel, Tiffany Myriam Delhomme, Patrice Hodonou Avogbe, Matthieu Foll, Farrokh Saidi, Hossein Poustchi, Masoud Sotoudeh, Reza Malekzadeh, Paul Brennan, James Mckay, Pierre Hainaut, Behnoush Abedi-Ardekani
Summary: Circulating cell-free DNA (cfDNA) is being recognized as a potential tumor biomarker, requiring high volumes of good quality plasma for extraction. Studies in high-risk areas of the Asian cancer belt show a certain concordance of TP53 gene mutations between tumor tissue and cfDNA extracted from archival serum, but the challenges lie in the high number of non-identifiable mutations.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Pathology
Lara Alessandrini, Elisabetta Zanoletti, Diego Cazzador, Marta Sbaraglia, Leonardo Franz, Giulia Tealdo, Anna Chiara Frigo, Stella Blandamura, Piero Nicolai, Antonio Mazzoni, Gino Marioni
Summary: This study highlights the significant role of tumor budding in the prognosis of TBSCC, showing associations with tumor invasion, lymph node metastasis, disease-free survival, and overall survival. Further research is needed to confirm these findings in larger prospective studies.
PATHOLOGY RESEARCH AND PRACTICE
(2022)
Article
Multidisciplinary Sciences
Huy Q. Dinh, Feng Pan, Geng Wang, Qing-Feng Huang, Claire E. Olingy, Zhi-Yong Wu, Shao-Hong Wang, Xin Xu, Xiu-E Xu, Jian-Zhong He, Qian Yang, Sandra Orsulic, Marcela Haro, Li-Yan Li, Guo-Wei Huang, Joshua J. Breunig, H. Phillip Koeffler, Catherine C. Hedrick, Li-Yan Xu, De-Chen Lin, En-Min Li
Summary: This study utilized single-cell RNA sequencing to characterize the heterogeneous microenvironment of ESCC, identifying a tumor-specific subset of CST1(+) myofibroblasts with prognostic values and immunosuppressive features. The analysis of stromal cell types in the ESCC microenvironment provides a rich resource for further understanding of ESCC biology.
NATURE COMMUNICATIONS
(2021)
Article
Oncology
Kosuke Nomura, Toshiro Iizuka, Daisuke Kaji, Yuki Asano-Mori, Yorinari Ochiai, Yugo Suzuki, Junnosuke Hayasaka, Satoshi Yamashita, Akira Matsui, Daisuke Kikuchi, Shuichi Taniguchi, Yutaka Takazawa, Shu Hoteya
Summary: The study found that approximately 60% of secondary ESCC cases after allo-HSCT were located in the cervical to upper thoracic esophagus. A history of TBI and bone marrow transplantation were identified as high-risk factors for ESCC, highlighting the importance of proactive screening endoscopy.
JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY
(2021)
Article
Dentistry, Oral Surgery & Medicine
Gabriela Vivili Domingues Silva, Eder da Silva Dolens, Livia Maris Ribeiro Paranaiba, Ana Lucia Carrinho Ayroza, Clarissa Araujo Gurgel Rocha, Alhadi Almangush, Tuula Salo, Peter A. Brennan, Ricardo D. Coletta
Summary: This study evaluated the prognostic impact of incorporating tumor-stroma ratio, tumor-infiltrating lymphocytes, and tumor budding into the World Health Organization histopathological grading for patients with oral squamous cell carcinoma. The findings suggest that tumor-stroma ratio may serve as a potential prognostic marker for oral squamous cell carcinoma, and the inclusion of tumor-infiltrating lymphocytes in the grading system improves its prognostic ability.
JOURNAL OF ORAL PATHOLOGY & MEDICINE
(2023)
Article
Pathology
Frido K. Bruehl, Erika E. Doxtader, Yu-Wei Cheng, Daniel H. Farkas, Carol Farver, Sanjay Mukhopadhyay
Summary: Histological assessment in distinguishing primary lung adenocarcinomas from intrapulmonary metastases can be inaccurate, leading to significant downstaging. Molecular analysis, especially driver gene mutations, provides a more reliable way to differentiate between related and unrelated tumor pairs, highlighting the importance of incorporating routine molecular findings in the diagnosis process.
JOURNAL OF CLINICAL PATHOLOGY
(2022)
Article
Hematology
Frido K. Bruehl, David S. Bosler, W. Scott Butsch, Daniel H. Farkas, Sarah L. Ondrejka
Summary: The majority of patients with unexplained cytopenia after BMS did not show specific bone marrow findings, caution is advised in interpreting dyserythropoiesis. Disturbances in micronutrient and vitamin levels, other than iron, folate, and vitamin B12, are common in this patient cohort and should be corrected with close clinical follow-up.
INTERNATIONAL JOURNAL OF LABORATORY HEMATOLOGY
(2022)
Article
Oncology
Marina Roy-Luzarraga, Louise E. Reynolds, Beatriz de Luxan-Delgado, Oscar Maiques, Laura Wisniewski, Emma Newport, Vinothini Rajeeve, Rebecca J. G. Drake, Jesus Gomez-Escudero, Frances M. Richards, Celine Weller, Christof Dormann, Ya-Ming Meng, Peter B. Vermeulen, Dieter Saur, Victoria Sanz-Moreno, Ping-Pui Wong, Cyrill Geraud, Pedro R. Cutillas, Kairbaan Hodivala-Dilke
Summary: This study reveals that endothelial cell focal adhesion kinase (FAK) plays a role in regulating pancreatic ductal adenocarcinoma (PDAC) metastasis and impacting the outcome of gemcitabine treatment. The findings suggest the potential clinical utility of combinatorial endothelial cell FAK targeting with gemcitabine in controlling metastasis in PDAC patients.
Article
Gastroenterology & Hepatology
Carolina J. Garcia Garcia, Yanqing Huang, Natividad R. Fuentes, Madeleine C. Turner, Maria E. Monberg, Daniel Lin, Nicholas D. Nguyen, Tara N. Fujimoto, Jun Zhao, Jaewon J. Lee, Vincent Bernard, Meifang Yu, Abagail M. Delahoussaye, Iancarlos Jimenez Sacarello, Emily G. Caggiano, Jae L. Phan, Amit Deorukhkar, Jessica M. Molkentine, Dieter Saur, Anirban Maitra, Cullen M. Taniguchi
Summary: In pancreatic ductal adenocarcinoma, stromal HIF2 appears to play a crucial role and represents a druggable target to alleviate tumor microenvironment immunosuppression and enhance immune responses.
Article
Biochemical Research Methods
Thorsten Kaltenbacher, Jessica Loeprich, Roman Maresch, Julia Weber, Sebastian Mueller, Rupert Oellinger, Nina Gross, Joscha Griger, Niklas de Andrade Kraetzig, Petros Avramopoulos, Deepak Ramanujam, Sabine Brummer, Sebastian A. Widholz, Stefanie Baerthel, Chiara Falcomata, Anja Pfaus, Ahmed Alnatsha, Julia Mayerle, Marc Schmidt-Supprian, Maximilian Reichert, Gunter Schneider, Ursula Ehmer, Christian J. Braun, Dieter Saur, Stefan Engelhardt, Roland Rad
Summary: This article describes a rapid and scalable genome engineering method for delivering CRISPR components into somatic cells of the liver and pancreas in living mice. It introduces various genetic tools, viral and nonviral CRISPR delivery strategies, and a range of applications including gene editing, cancer modeling, chromosome engineering, and spatio-temporal control of CRISPR multiplexing. The protocol also discusses quantification methods for genetic and functional editing outcomes, as well as data analysis and interpretation. The use of this protocol takes 3-5 weeks depending on the delivery method and intended application.
Article
Oncology
Tanja Groll, Miguel Silva, Rim Sabrina Jahan Sarker, Markus Tschurtschenthaler, Theresa Schnalzger, Carolin Mogler, Daniela Denk, Sebastian Schoelch, Barbara U. Schraml, Jurgen Ruland, Roland Rad, Dieter Saur, Wilko Weichert, Moritz Jesinghaus, Kaspar Matiasek, Katja Steiger
Summary: The study found that the number of ieMMCs decreases as the disease progresses from low-grade adenomas to carcinomas in intestinal cancer, and that ieMMC scores were higher in mouse models with altered Wnt signaling compared to models with altered MAPK signaling. ieMMCs play an important role in the tumor microenvironment, particularly in relation to the onset and progression of intestinal cancer.
Article
Oncology
Alexander Scheiter, Frederik Hierl, Florian Lueke, Felix Keil, Daniel Heudobler, Sabine Einhell, Margit Klier-Richter, Nikola P. Konstandin, Florian Weber, Andrea Scheiter, Arne Kandulski, Sophie Schlosser, Lidia-Sabina Cosma, Hauke Tews, Andreas R. R. Weiss, Matthias Grube, Elisabeth Bumes, Peter Hau, Martin Proescholdt, Felix Steger, Anja Troeger, Sebastian Haferkamp, Lucas E. Reibenspies, Marco J. Schnabel, Christian Schulz, Konstantin Drexler, Maria E. Hatzipanagiotou, Stephan Seitz, Monika Klinkhammer-Schalke, Philipp Unberath, Diego F. Calvisi, Tobias Pukrop, Wolfgang Dietmaier, Matthias Evert, Kirsten Utpatel
Summary: This study evaluated the outcomes of molecular tumor boards in a comprehensive cancer center. The results showed that the recommendation rate was lower for colorectal cancer and pancreatic cancer, indicating the need for a weighted prioritization of different cancers and the implementation of high-tier recommendations.
BRITISH JOURNAL OF CANCER
(2023)
Article
Biochemistry & Molecular Biology
Christian Schneeweis, Sandra Diersch, Zonera Hassan, Lukas Krauss, Carolin Schneider, Daniele Lucarelli, Chiara Falcomata, Katja Steiger, Rupert Oellinger, Oliver H. Kraemer, Alexander Arlt, Marian Grade, Marc Schmidt-Supprian, Elisabeth Hessmann, Matthias Wirth, Roland Rad, Maximilian Reichert, Dieter Saur, Guenter Schneider
Summary: This study identifies FRA1 as a regulator of sensitivity to RAF-MEK-ERK inhibitors in pancreatic cancer. FRA1 is associated with the adaptive rewiring of oncogenic ERK signaling and its degradation synergizes with MEK inhibitors.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2023)
Review
Oncology
Chiara Falcomata, Stefanie Baerthel, Guenter Schneider, Roland Rad, Marc Schmidt-Supprian, Dieter Saur
Summary: Pancreatic cancer exhibits universal immunosuppression, but its tumor entity is highly heterogeneous across different subtypes and phenotypes. Understanding the context-specific modes of immunosuppression will aid in the development of more effective multimodal (immuno)therapeutic interventions for targeting pancreatic cancer.
Review
Oncology
Stefanie Baerthel, Chiara Falcomata, Roland Rad, Fabian J. Theis, Dieter Saur
Summary: Pancreatic ductal adenocarcinoma (PDAC) is a highly lethal cancer with a heterogeneous genetic landscape and an immunosuppressive tumor microenvironment. Recent advances in single-cell sequencing and spatial transcriptomics have provided insights into the diversity and plasticity of PDAC, both in its malignant cells and the surrounding tissue. This review highlights the importance of single-cell analysis in understanding PDAC and discusses the potential of multimodal approaches to study its biology and response to therapy.
Article
Oncology
Tina Sket, Chiara Falcomata, Dieter Saur
Summary: The combination of next-generation sequencing (NGS) and single-cell profiling technologies has allowed us to uncover the complex and diverse ecosystem of human tumors. Genetically engineered mouse models (GEMMs) of human cancers based on dual-recombinase system (DRS) provide new opportunities to study and understand the key drivers of tumor phenotypes and validate potential therapeutic targets. In this article, we discuss how DRS-based cancer GEMMs can be used to model, trace, manipulate, and investigate established tumors, their interactions with the host, and their response to therapy.
Letter
Cell Biology
Frido K. Bruehl, Andrew P. Norgan, Min Shi, Karen L. Rech, Yanna Ding, Stefania Pittaluga, Ji Yuan
Article
Hematology
Frido K. Bruehl, Rhett P. Ketterling, Lisa M. Rimsza, Edward F. Santos, Ellen D. McPhail
Summary: Classification of DLBCL relies on clinical, immunohistochemical, and genetic information. We present a case of PT-DLBCL with an unreported constellation of pathologic findings, illustrating the challenges of DLBCL classification. Genetic testing showed MYD88 mutation, IGH::MYC and IRF4- and BCL6-rearrangements, and gene expression profiling demonstrated an activated B-cell expression profile.
JOURNAL OF HEMATOPATHOLOGY
(2023)
Article
Hematology
Frido K. Bruehl, Mazen M. Osman, Dong Chen, Joanna C. Dalland
Summary: The 2022 ICC and WHO 5th edition classifications have proposed significant changes to the diagnostic criteria of MDS. This study evaluated their impact on the diagnosis of cytopenias and MDS. The results showed major changes in the diagnosis according to both classification systems, with some differences in reclassification of specific MDS entities. It is important to assess the potential diagnostic and clinical impacts before implementing these new classification systems.
JOURNAL OF HEMATOPATHOLOGY
(2023)
Article
Radiology, Nuclear Medicine & Medical Imaging
Hui Wang, Marianne Remke, Thomas Horn, Kristina Schwamborn, Yiyao Chen, Katja Steiger, Wilko Weichert, Hans-Juergen Wester, Margret Schottelius, Wolfgang A. Weber, Matthias Eiber
Summary: PSMA expression and PSMA-ligand uptake show marked heterogeneity. Prostate carcinoma with GP 4 showed significantly higher uptake compared with non-neoplastic prostate tissue.