Article
Multidisciplinary Sciences
Adarsh Kumar, Andrew J. Reed, Walter J. Zahurancik, Sasha M. Daskalova, Sidney M. Hecht, Zucai Suo
Summary: Base excision repair (BER) is a crucial cellular pathway for DNA damage repair, and DNA polymerase beta (Pol beta) plays a key role in this process. This study reveals the sequential order of two enzymatic activities of Pol beta during BER and identifies the intermediate molecules involved. The coordinated activities of Pol beta's active sites are found to be critical for the BER pathway, suggesting potential alternative strategies for therapeutic intervention.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biochemistry & Molecular Biology
Marina Jordano-Raya, Cristina Beltran-Melero, M. Dolores Moreno-Recio, M. Isabel Martinez-Macias, Rafael R. Ariza, Teresa Roldan-Arjona, Dolores Cordoba-Canero
Summary: The study investigated the influence of sequence context on the activity of plant and human AP endonucleases and AP lyases on AP sites opposite G or C, revealing differential preferences and efficiencies of these enzymes in different situations. The findings suggest that plant AP endonucleases and AP lyases may play complementary roles in neutralizing the mutagenic consequences of C deamination and G oxidation on abasic sites formed at C:G pairs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Multidisciplinary
Yong Woong Jun, Eddy Albarran, David L. Wilson, Jun Ding, Eric T. Kool
Summary: Mitochondrial function declines with aging and neurodegeneration, largely due to accumulated mutations in mtDNA from deficient DNA repair. A fluorescent probe, UBER, was used to visualize mitochondrial BER activity, showing increased repair under oxidative stress and time delay between stress onset and BER initiation. Cells lacking MTH1 exhibited elevated demand for BER activity during extended stress, while absence of OGG1 limited glycosylation capacity.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Biochemistry & Molecular Biology
Brittany L. Carroll, Karl E. Zahn, John P. Hanley, Susan S. Wallace, Julie A. Dragon, Sylvie Doublie
Summary: Base excision repair (BER) is a key pathway in protecting cells from DNA damage caused by reactive oxygen species, with NTHL1 being a bifunctional DNA glycosylase that plays a role in repairing specific base damage; The X-ray crystal structure of hNTHL1 reveals an open conformation not seen in bacterial orthologs, with the flexibility and configuration change attributed to an interdomain linker; The large scale interdomain rearrangement during catalysis observed in hNTHL1 is unprecedented for a DNA glycosylase in the HhH superfamily, providing important insights into the molecular mechanism of this enzyme.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Chemistry, Physical
A. K. Khedr, R. R. Zaky, E. A. Gomaa, M. N. Abd El-Hady
Summary: A series of transition metal complexes were prepared using a novel ligand and various metal cations, and their structures, properties, and biological activities were extensively studied.
JOURNAL OF MOLECULAR LIQUIDS
(2022)
Article
Multidisciplinary Sciences
Nicholas A. W. Bell, Justin E. Molloy
Summary: In this study, a new experimental method was developed to measure the mechanical stability and interaction kinetics of proteins across the two ends of a DNA double-strand break. It was found that PARP2 forms a stable mechanical link across blunt-end 5;- phosphorylated DSBs and restores torsional continuity. In contrast, PARP1 was not observed to form a bridging interaction across blunt or short overhang DSBs and competed away PARP2 bridge formation.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Chemistry, Medicinal
Shu-Ping Wang, Yu Li, Shi-Hui Huang, Shi-Qi Wu, Ling-Li Gao, Qin Sun, Qian-Wen Lin, Lei Huang, Liu-Qiong Meng, Yi Zou, Qi-Hua Zhu, Yun-Gen Xu
Summary: Targeting PARP1/2 and BRD4 with the novel dual inhibitor III-16 showed promising synergistic antitumor efficacy in pancreatic cancer, suggesting it as a potential treatment option for advanced pancreatic cancer.
JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Michal R. Szymanski, Anna Karlowicz, Geoffrey K. Herrmann, Yana Cen, Y. Whitney Yin
Summary: This study investigates the role of human mitochondrial EXOG (hEXOG) in the repair of oxidative damage on mitochondrial DNA. The research shows that hEXOG has strong 5'-dRP removal ability and can function on various abasic sites. The study also provides structural insights into how hEXOG sets the order of repair reactions and inhibits off-pathway reactions.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Article
Cell Biology
Joost Schimmel, Nuria Munoz-Subirana, Hanneke Kool, Robin van Schendel, Sven van der Vlies, Juliette A. Kamp, Femke de Vrij, Steven A. Kushner, Graeme C. M. Smith, Simon J. Boulton, Marcel Tijsterman
Summary: Gene editing can be improved by inhibiting non-homologous end joining (NHEJ) or polymerase theta-mediated end joining (TMEJ), which alters the outcomes of CRISPR-Cas9. Inhibiting TMEJ activity using ART558 suppresses microhomology-driven deletions and promotes NHEJ-specific outcomes. Combining TMEJ and NHEJ inhibition increases the efficiency of precise gene editing through homology-driven repair (HDR).
Article
Chemistry, Inorganic & Nuclear
Khlood S. Abou-Melha
Summary: A new series of Co(II), Cu(II) and Zn(II) complexes were synthesized with specific spectroscopic methods used to investigate the structure. Different coordination modes of the ligand in different metal ions, thermal degradation curves and biological activities of the complexes were studied, revealing unique properties of the synthesized compounds.
INORGANIC CHEMISTRY COMMUNICATIONS
(2021)
Article
Biology
Yuji Seo, Keisuke Tamari, Yutaka Takahashi, Kazumasa Minami, Shotaro Tatekawa, Fumiaki Isohashi, Osamu Suzuki, Yuichi Akino, Kazuhiko Ogawa
Summary: PARPi has the potential to induce radiosensitization, enhancing the β-component of the linear-quadratic model. It shows notable radiosensitizing effects in high-dose-per-fraction radiotherapy. PARPi alters the choice of DNA repair pathways rather than reducing the total amount of DNA repair, resulting in increased radiosensitization.
INTERNATIONAL JOURNAL OF RADIATION BIOLOGY
(2022)
Article
Gastroenterology & Hepatology
Johann Gout, Lukas Perkhofer, Mareen Morawe, Frank Arnold, Michaela Ihle, Stephanie Biber, Sebastian Lange, Elodie Roger, Johann M. Kraus, Katja Stifter, Stephan A. Hahn, Andrea Zamperone, Thomas Engleitner, Martin Mueller, Karolin Walter, Eva Rodriguez-Aznar, Bruno Sainz Jr, Patrick C. Hermann, Elisabeth Hessmann, Sebastian Mueller, Ninel Azoitei, Andre Lechel, Stefan Liebau, Martin Wagner, Diane M. Simeone, Hans A. Kestler, Thomas Seufferlein, Lisa Wiesmueller, Roland Rad, Pierre-Olivier Frappart, Alexander Kleger
Summary: The study identified synergistic effects in inhibiting PARP, ATR, and DNA-PKcs that lead to synthetic lethality in ATM-deficient pancreatic ductal adenocarcinoma. Chemical inhibition of ATM sensitized human PDAC cells towards this combinatorial therapy, which was also found to overcome PARP inhibitor resistance. These findings suggest potential mutation-specific therapeutic strategies that may be applied through ATM inhibition regardless of genotype.
Article
Biochemistry & Molecular Biology
Anton Endutkin, Anna Yudkina, Timofey D. Zharkov, Daria Kim, Dmitry O. Zharkov
Summary: Azide-alkyne cycloaddition (click chemistry) is widely used for analyzing molecular interactions in living cells. A recently developed apurinic/apyrimidinic (AP) site analog, 5-ethynyl-2-(hydroxymethyl)tetrahydrofuran-3-ol (EAP), can be introduced into cells to label or cross-link DNA constructs. However, EAP may be removed by DNA repair or misread by DNA polymerases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Inga R. Grin, Daria V. Petrova, Anton V. Endutkin, Chunquan Ma, Bing Yu, Haiying Li, Dmitry O. Zharkov
Summary: Base excision DNA repair is an important pathway in safeguarding the genome of plants, and understanding their repair mechanisms is crucial for developing new crop varieties.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Jamie E. DeNizio, Blaine J. Dow, Juan C. Serrano, Uday Ghanty, Alexander C. Drohat, Rahul M. Kohli
Summary: Cytosine methylation primarily occurs in CG dinucleotide contexts in mammalian genomes. However, the active DNA demethylation pathway involving TET and TDG enzymes is not limited to CG sites and can also affect CH sites, providing a rationale for the depletion of hmCH in genomes rich in mCH.
JOURNAL OF MOLECULAR BIOLOGY
(2021)
Article
Plant Sciences
Nadezhda Dyrkheeva, Olga Luzina, Aleksandr Filimonov, Olga Zakharova, Ekaterina Ilina, Alexandra Zakharenko, Maxim Kuprushkin, Dmitry Nilov, Irina Gushchina, Vytas Svedas, Nariman Salakhutdinov, Olga Lavrik
Article
Cell Biology
Anastasiya A. Kosova, Mikhail M. Kutuzov, Alexei N. Evdokimov, Ekaterina S. Ilina, Ekaterina A. Belousova, Svetlana A. Romanenko, Vladimir A. Trifonov, Svetlana N. Khodyreva, Olga Lavrik
Article
Biochemistry & Molecular Biology
Maria V. Sukhanova, Loic Hamon, Mikhail M. Kutuzov, Vandana Joshi, Sanae Abrakhi, Ioana Dobra, Patrick A. Curmi, David Pastre, Olga I. Lavrik
JOURNAL OF MOLECULAR BIOLOGY
(2019)
Article
Biochemistry & Molecular Biology
Tatyana M. Khomenko, Alexandra L. Zakharenko, Arina A. Chepanova, Ekaterina S. Ilina, Olga D. Zakharova, Vasily I. Kaledin, Valeriy P. Nikolin, Nelly A. Popova, Dina V. Korchagina, Johannes Reynisson, Raina Chand, Daniel M. Ayine-Tora, Jinal Patel, Ivanhoe K. H. Leung, Konstantin P. Volcho, Nariman F. Salakhutdinov, Olga I. Lavrik
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Biochemistry & Molecular Biology
Yuliya V. Sherstyuk, Nikita V. Ivanisenko, Alexandra L. Zakharenko, Maria V. Sukhanova, Roman Y. Peshkov, Ilia V. Eltsov, Mikhail M. Kutuzov, Tatiana A. Kurgina, Ekaterina A. Belousova, Vladimir A. Ivanisenko, Olga I. Lavrik, Vladimir N. Silnikov, Tatyana V. Abramova
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Biochemistry & Molecular Biology
Dmitry Nilov, Natalya Maluchenko, Tatyana Kurgina, Sergey Pushkarev, Alexandra Lys, Mikhail Kutuzov, Nadezhda Gerasimova, Alexey Feofanov, Vytas Svedas, Olga Lavrik, Vasily M. Studitsky
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Plant Sciences
Olga Luzina, Alexander Filimonov, Alexandra Zakharenko, Arina Chepanova, Olga Zakharova, Ekaterina Ilina, Nadezhda Dyrkheeva, Galina Likhatskaya, Nariman Salakhutdinov, Olga Lavrik
JOURNAL OF NATURAL PRODUCTS
(2020)
Article
Biochemistry & Molecular Biology
Irina Il'ina, Nadezhda S. Dyrkheeva, Alexandra L. Zakharenko, Alexander Yu Sidorenko, Nikolay S. Li-Zhulanov, Dina Korchagina, Raina Chand, Daniel M. Ayine-Tora, Arina A. Chepanova, Olga D. Zakharova, Ekaterina S. Ilina, Johannes Reynisson, Anastasia A. Malakhova, Sergey P. Medvedev, Suren M. Zakian, Konstantin P. Volcho, Nariman F. Salakhutdinov, Olga Lavrik
Article
Biochemistry & Molecular Biology
Alexandra L. Zakharenko, Mikhail S. Drenichev, Nadezhda S. Dyrkheeva, Georgy A. Ivanov, Vladimir E. Oslovsky, Ekaterina S. Ilina, Irina A. Chernyshova, Olga Lavrik, Sergey N. Mikhailov
Article
Biochemistry & Molecular Biology
Elizaveta D. Gladkova, Ivan Nechepurenko, Roman A. Bredikhin, Arina A. Chepanova, Alexandra L. Zakharenko, Olga A. Luzina, Ekaterina S. Ilina, Nadezhda S. Dyrkheeva, Evgeniya M. Mamontova, Rashid O. Anarbaev, Johannes Reynisson, Konstantin P. Volcho, Nariman E. Salakhutdinov, Olga Lavrik
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Biochemistry & Molecular Biology
Konstantin N. Naumenko, Mariya Sukhanova, Loic Hamon, Tatyana A. Kurgina, Elizaveta E. Alemasova, Mikhail M. Kutuzov, David Pastre, Olga Lavrik
Article
Multidisciplinary Sciences
M. M. Kutuzov, E. A. Belousova, T. A. Kurgina, A. A. Ukraintsev, I. A. Vasil'eva, S. N. Khodyreva, O. I. Lavrik
Summary: The study revealed that the presence of PARP1 leads to the suppression of BER enzymes' activities, while PARP2 predominantly influences the last stage of BER. PARylation attenuates the inhibitory effect of PARP1, but results in Pol beta inhibition and significant stimulation of LigIII alpha by PARP2 in a NAD(+)-dependent manner.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Inna Vasil'eva, Nina Moor, Rashid Anarbaev, Mikhail Kutuzov, Olga Lavrik
Summary: The study found that PARP2 forms more dynamic complexes with other protein partners in base excision repair, and their stability is influenced by DNA intermediates. PARP2 demonstrates stronger self-regulation of PAR elongation at high PARP and NAD(+) concentrations compared to PARP1, and is more effectively inhibited by XRCC1.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biology
Tatyana A. Kurgina, Nina A. Moor, Mikhail M. Kutuzov, Konstantin N. Naumenko, Alexander A. Ukraintsev, Olga Lavrik
Summary: The histone PARylation factor 1 (HPF1) has been found to stimulate the autoPARylation of PARP1 and PARP2 as well as the heteroPARylation of histones in the complex with nucleosome. The stimulatory action is dependent on the concentrations of HPF1 and NAD(+), with PARP2 showing more efficiency than PARP1 in the reaction. The dual function of HPF1 in maintaining PARP activity is also discussed.
COMMUNICATIONS BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Siva Santhiya Arul, Brinda Balakrishnan, Savithri S. Handanahal, Sangita Venkataraman
Summary: Viral nanoparticles are self-assembling delivery systems used for vaccines and therapeutic agents. They have improved biocompatibility and are widely used in various biomedical applications. However, large-scale production of VNPs still faces challenges.
Article
Biochemistry & Molecular Biology
Wisely Chua, Carl O. Marsh, Si En Poh, Winston LC. Koh, Melody Li Ying Lee, Li Fang Koh, Xin-Zi Emily Tang, Peter See, Zheng Ser, Shi Mei Wang, Radoslaw M. Sobota, Thomas L. Dawson Jr, Yik Weng Yew, Steven Thng, Anthony J. O'Donoghue, Hazel H. Oon, John E. Common, Hao Li
Summary: In this study, the gene expression of enzymes secreted by Malassezia globosa in the skin was investigated using targeted RNA sequencing. The study found that the expression of these enzymes is regulated by the fungus's environment and differs significantly from healthy skin sites. Additionally, a pseudoprotease MGL_3331 was identified to elicit an immune reaction in patients with atopic dermatitis. This highlights the importance of studying fungal proteins in physiologically relevant environments and their role in host immunity.
Article
Biochemistry & Molecular Biology
Belisa Parmeggiani, Marian Flores Signori, Cristiane Cecatto, Marina Rocha Frusciante, Manuela Bianchin Marcuzzo, Debora Guerini Souza, Rafael Teixeira Ribeiro, Bianca Seminotti, Diogo Onofre Gomes de Souza, Cesar Augusto Joa o Ribeiro, Moacir Wajner, Guilhian Leipnitz
Summary: Non ketotic hyperglycinemia (NKH) is a disorder caused by mutations in the genes encoding glycine cleavage system proteins, leading to severe neurodegeneration and white matter alterations. The study found that glycine affects myelin structure, glutamatergic system, and induces oxidative stress in neonatal rats and MO3.13 oligodendroglial cells.
Review
Biochemistry & Molecular Biology
Marie Rose Ndella Thiaw, Pascal Gantet
Summary: Mini zinc fingers play important roles in plant development, including control of cell division and expansion, meristem state transition, and growth and organ development. They have diverse modes of action, such as direct gene activation, interaction with specific transcription factors, and aggregation of protein complexes for chromatin remodeling. Further research is needed to explore their wider range of biological functions and their involvement in biotic and abiotic stress responses.
Article
Biochemistry & Molecular Biology
Shao-Jui Lai, Takahiro Kameda, Maasa Morita, Yuka Yamagata, Kaoruko Nishizaka, Yuna Horiuchi, Yukihiro Kobayashi, Yoko Usami, Jun-Jen Liu, Takeshi Kasama, Minoru Tozuka, Ryunosuke Ohkawa
Summary: In this study, the co-modification and impact of chymase and MPO on HDL were investigated. Sequential treatment with MPO and chymase generated two novel apoA-I fragments from HDL, one of which has a specific immunological property and can be used to predict patients with normal HDL-C levels and cardiovascular diseases.
Article
Biochemistry & Molecular Biology
Xiao-Mi Sun, Asami Yoshida, Takuya Ishii, Yan-Rong Jiang, Yi-Li Gao, Mikinori Ueno, Katsuya Hirasaka, Kiyoshi Osatomi
Summary: This study found that infection with the causative bacteria of edwardsiellosis, Edwardsiella tarda NUF251, leads to the production of superoxide radicals, causing oxidative stress. The research on the Jfsod1 gene suggests that the transcription factor NF-IL6 plays a significant role in upregulating the transcriptional activity of the gene in response to oxidative stress induced by NUF251 infection.
Article
Biochemistry & Molecular Biology
Shane M. Carey, Sean P. Kearns, Matthew E. Millington, Gregory S. Buechner, Beda E. Alvarez Jr, Leily Daneshian, Brendan Abiskaroon, Maksymilian Chruszcz, Edward L. D'Antonio
Summary: The importance of F337 in TcGlcK was investigated using the hole mutagenesis approach, revealing its key interactions with aromatic tail groups in inhibitor molecules. Mutants retaining activity and structural similarity were obtained, suggesting the possibility of TcHxK existing as a monomer lacking the F337 residue. Similar D-glucosamine inhibitors may bind less strongly to TcHxK due to the absence of van der Waals contacts from residue side chains.
Article
Biochemistry & Molecular Biology
Catia Barria, Diogo Athayde, Guillem Hernandez, Leonor Fonseca, Jorge Casinhas, Tiago N. Cordeiro, Margarida Archer, Cecilia M. Arraiano, Jose A. Brito, Rute G. Matos
Summary: Ribonucleases play a crucial role in RNA regulation by processing, degrading, and quality controlling cellular transcripts. Campylobacter jejuni, the main cause of human gastroenteritis, relies on the exoribonuclease PNPase (CjPNP) for low-temperature survival, virulence factor synthesis, and swimming, cell adhesion/invasion, and chick colonization abilities. The crystallographic structure of CjPNP, along with SAXS analysis, confirms its trimeric arrangement and provides insight into domain arrangement and flexibility. Mutations in highly conserved residues reveal unexpected RNA degradation activity even under conditions favoring polymerization. These findings have significant implications for the development of strategies to combat C. jejuni infections.
Article
Biochemistry & Molecular Biology
Elvira R. Shaykhutdinova, Maria S. Severyukhina, Inna V. Kholoshenko, Elena A. Gondarenko, Irina V. Shelukhina, Elena V. Kryukova, Alina M. Ismailova, Elena S. Sadovnikova, Igor A. Dyachenko, Arkady N. Murashev, Victor I. Tsetlin, Yuri N. Utkin
Summary: Evidence suggests that activating nicotinic acetylcholine receptors (nAChRs) can reduce cardiac injury caused by ischemia and reperfusion. In this study, the expression of a4, a6, and a132 nAChR subunits in the rat heart's left ventricle was demonstrated. The nAChR agonists cytisine and varenicline, commonly used for nicotine addiction treatment, were found to significantly reduce myocardial ischemia-reperfusion injury, with varenicline showing greater protection. The presence of a4132, a7, and/or a6132-containing receptors suggests that the protective effect of varenicline may be mediated by these subunits. These findings indicate a potential new use for cytisine and varenicline as cardioprotective agents.
Article
Biochemistry & Molecular Biology
Xinhai Xiong, Wenjie Chen, Cheng Chen, Qi Wu, Chaopeng He
Summary: This article summarizes the important roles of Cx43 in disease development from the perspective of subcellular localization and provides new ideas for Cx43 as a therapeutic target and the search for related pathological mechanisms.
Article
Biochemistry & Molecular Biology
Veronica Bastos, Simone Pascoal, Kamila Lopes, Marcia Mortari, Helena Oliveira
Summary: Cancer, especially melanoma, is a major global health problem due to its metastatic capacity, treatment resistance, and high mortality rates. The Chartergellus-CP1 peptide isolated from Chartergellus communis wasp venom showed selective cytotoxicity towards melanoma cell lines, inducing apoptosis, cell cycle impairment, and intracellular ROS increase.
Article
Biochemistry & Molecular Biology
Charlotte Claeyssen, Nathan Bulangalire, Bruno Bastide, Onnik Agbulut, Caroline Cieniewski-Bernard
Summary: Maintenance of striated muscle tissue relies on a dynamic network of protein-protein interactions. Desmin intermediate filaments play a crucial role in heart and skeletal muscle by maintaining cell structure and contributing to various cellular processes. The assembly of desmin intermediate filaments requires aB-crystallin, which is involved in multiple cellular functions.
Article
Biochemistry & Molecular Biology
Dhritiman Dey, Adhiraj Dasgupta, Dipanjan Ghosh, Oindrila Bhattacharjee, Abhrajyoti Ghosh, Ayae Honda, Dhrubajyoti Chattopadhyay
Summary: This study successfully identified host factors that interact with Chandipura Virus (CHPV) and play a critical role in infection, including A2M, LRP1, and GRP78. Knocking out A2M has a severe effect on viral infection, indicating the potential role of these host proteins in viral entry into host cells.
Article
Biochemistry & Molecular Biology
Anna Dotsenko, Igor Sinelnikov, Aleksandra Rozhkova, Ivan Zorov, Arkady Sinitsyn
Summary: Xylanases play important roles in various industrial applications, such as feed additives, pulp and paper bleaching, and the production of bread, food, and drinks. Xylanases from the GH10 family show high thermostability and resistance to protein inhibitors, making them promising candidates for these processes. This study successfully improved the thermostability and enzymatic activity of GH10 xylanase, which could enhance its effectiveness as a feed additive and during pulp and paper bleaching.
Article
Biochemistry & Molecular Biology
M. Jaquet, M. Bengue, K. Lambert, G. Carnac, D. Misse, C. Bisbal
Summary: Changes to our environment have led to the emergence of human pathogens such as chikungunya virus, which is now a major public health concern. In this study, the interconnection between skeletal muscle cells metabolism, their differentiation stage, and the infectivity of chikungunya virus was investigated. The results showed that chikungunya virus infectivity is dependent on cell differentiation and metabolism and that the virus interferes with cellular metabolism.