High-performance gene expression and knockout tools using sleeping beauty transposon system

Phase I trials using Sleeping Beauty to generate CD19-specific CAR T cells

(2016) Partow Kebriaei et al.   JOURNAL OF CLINICAL INVESTIGATION

FBXW7 Facilitates Nonhomologous End-Joining via K63-Linked Polyubiquitylation of XRCC4

(2016) Qiang Zhang et al.   MOLECULAR CELL

High-fidelity CRISPR–Cas9 nucleases with no detectable genome-wide off-target effects

(2016) Benjamin P. Kleinstiver et al.   NATURE

NFATc1 regulates cell proliferation, migration, and invasion of ovarian cancer SKOV3 cells in vitro and in vivo

(2016) Long Li et al.   ONCOLOGY REPORTS

Transposon-mediated generation of BCR-ABL1-expressing transgenic cell lines for unbiased sensitivity testing of tyrosine kinase inhibitors

(2016) Konstantin Byrgazov et al.   Oncotarget

ASK1 controls spindle orientation and positioning by phosphorylating EB1 and stabilizing astral microtubules

(2016) Youguang Luo et al.   Cell Discovery

A vector platform for the rapid and efficient engineering of stable complex transgenes

(2016) Carsten Jäckel et al.   Scientific Reports

Optimized Sleeping Beauty transposons rapidly generate stable transgenic cell lines

(2015) Eric Kowarz et al.   Biotechnology Journal

Comprehensive Ex Vivo Transposon Mutagenesis Identifies Genes That Promote Growth Factor Independence and Leukemogenesis

(2015) Y. Guo et al.   CANCER RESEARCH

NFATc1 activation promotes the invasion of U251 human glioblastoma multiforme cells through COX-2

(2015) LAIZANG WANG et al.   INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE

Rationally engineered Cas9 nucleases with improved specificity

(2015) I. M. Slaymaker et al.   SCIENCE

Optimized Sleeping Beauty transposons rapidly generate stable transgenic cell lines

(2015) Eric Kowarz et al.   Biotechnology Journal

Off-target Effects in CRISPR/Cas9-mediated Genome Engineering

(2015) Xiao-Hui Zhang et al.   Molecular Therapy-Nucleic Acids

Sleeping Beautytransposon system for genetic etiological research and gene therapy of cancers

(2014) Xiaomei Hou et al.   CANCER BIOLOGY & THERAPY

Functional characterisation of different MLL fusion proteins by using inducible Sleeping Beauty vectors

(2014) K. Wächter et al.   CANCER LETTERS

Improving CRISPR-Cas nuclease specificity using truncated guide RNAs

(2014) Yanfang Fu et al.   NATURE BIOTECHNOLOGY

Dimeric CRISPR RNA-guided FokI nucleases for highly specific genome editing

(2014) Shengdar Q Tsai et al.   NATURE BIOTECHNOLOGY

Genome-wide analysis reveals characteristics of off-target sites bound by the Cas9 endonuclease

(2014) Cem Kuscu et al.   NATURE BIOTECHNOLOGY

Improved vectors and genome-wide libraries for CRISPR screening

(2014) Neville E Sanjana et al.   NATURE METHODS

CYLD regulates spindle orientation by stabilizing astral microtubules and promoting dishevelled-NuMA-dynein/dynactin complex formation

(2014) Yunfan Yang et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

The ubiquitin proteasome system — Implications for cell cycle control and the targeted treatment of cancer

(2013) Florian Bassermann et al.   BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH

Acquired Expression of NFATc1 Downregulates E-Cadherin and Promotes Cancer Cell Invasion

(2013) T. Oikawa et al.   CANCER RESEARCH

TD-60 is required for interphase cell cycle progression

(2013) Mythili Yenjerla et al.   CELL CYCLE

High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells

(2013) Yanfang Fu et al.   NATURE BIOTECHNOLOGY

Gateway-compatible transposon vector to genetically modify human embryonic kidney and adipose-derived stromal cells.

(2012) Spyros Petrakis et al.   Biotechnology Journal

NFAT control of innate immunity

(2012) J. Fric et al.   BLOOD

CHK1 targets spleen tyrosine kinase (L) for proteolysis in hepatocellular carcinoma

(2012) Jian Hong et al.   JOURNAL OF CLINICAL INVESTIGATION

A Sleeping Beauty mutagenesis screen reveals a tumor suppressor role for Ncoa2/Src-2 in liver cancer

(2012) K. A. O'Donnell et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

The Sleeping Beauty transposon system: a non-viral vector for gene therapy

(2011) E. L. Aronovich et al.   HUMAN MOLECULAR GENETICS

TNF activates calcium-nuclear factor of activated T cells (NFAT)c1 signaling pathways in human macrophages

(2011) A. Yarilina et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

The Fbw7 Tumor Suppressor Targets KLF5 for Ubiquitin-Mediated Degradation and Suppresses Breast Cell Proliferation

(2010) Dong Zhao et al.   CANCER RESEARCH

Nonproteolytic Functions of Ubiquitin in Cell Signaling

(2009) Zhijian J. Chen et al.   MOLECULAR CELL

A conditional transposon-based insertional mutagenesis screen for genes associated with mouse hepatocellular carcinoma

(2009) Vincent W Keng et al.   NATURE BIOTECHNOLOGY

Molecular evolution of a novel hyperactive Sleeping Beauty transposase enables robust stable gene transfer in vertebrates

(2009) Lajos Mátés et al.   NATURE GENETICS

Isoform- and cell cycle–dependent substrate degradation by the Fbw7 ubiquitin ligase

(2008) Jonathan E. Grim et al.   JOURNAL OF CELL BIOLOGY