Innovative Immunization Strategies for Antivenom Development

Short-chain consensus alpha-neurotoxin: a synthetic 60-mer peptide with generic traits and enhanced immunogenic properties

(2018) Guillermo de la Rosa et al.   AMINO ACIDS

Cloning and sequencing of three-finger toxins from the venom glands of four Micrurus species from Mexico and heterologous expression of an alpha-neurotoxin from Micrurus diastema

(2018) Jaime Felipe Guerrero-Garzón et al.   BIOCHIMIE

Vulnerability to snakebite envenoming: a global mapping of hotspots

(2018) Joshua Longbottom et al.   LANCET

Current technology for the industrial manufacture of snake antivenoms

(2018) Guillermo León et al.   TOXICON

Pros and cons of different therapeutic antibody formats for recombinant antivenom development

(2018) Andreas H. Laustsen et al.   TOXICON

Developing recombinant phospholipase D1 (rPLD1) toxoid from Iranian Hemiscorpius lepturus scorpion and its protective effects in BALB/c mice

(2018) Narges Safari Foroushani et al.   TOXICON

Toxin-centric development approach for next-generation antivenoms

(2018) Andreas H. Laustsen   TOXICON

Third generation antivenomics: Pushing the limits of the in vitro preclinical assessment of antivenoms

(2018) D. Pla et al.   TOXICON

Immunization with lipopolysaccharide-free outer membrane complexes protects against Acinetobacter baumannii infection

(2018) Marina R. Pulido et al.   VACCINE

Tumor lysate-loaded Bacterial Ghosts as a tool for optimized production of therapeutic dendritic cell-based cancer vaccines

(2018) N. Dobrovolskienė et al.   VACCINE

Cyclic OmpC peptidic epitope conjugated to tetanus toxoid as a potential vaccine candidate against shigellosis

(2018) Anna Jarząb et al.   VACCINE

DNA prime/MVTT boost regimen with HIV-1 mosaic Gag enhances the potency of antigen-specific immune responses

(2018) Wan Liu et al.   VACCINE

Design and immune characterization of a novel Neisseria gonorrhoeae DNA vaccine using bacterial ghosts as vector and adjuvant

(2018) Hongmei Jiao et al.   VACCINE

Antivenom Production against Bothrops jararaca and Bothrops erythromelas Snake Venoms Using Cross-Linked Chitosan Nanoparticles as an Immunoadjuvant

(2018) Karla Soares et al.   Toxins

Antimicrobial Peptides and Nanotechnology, Recent Advances and Challenges

(2018) Lubhandwa S. Biswaro et al.   Frontiers in Microbiology

New Routes and Opportunities for Modular Construction of Particulate Vaccines: Stick, Click, and Glue

(2018) Karl D. Brune et al.   Frontiers in Immunology

Recombinant Protein Containing B-Cell Epitopes of Different Loxosceles Spider Toxins Generates Neutralizing Antibodies in Immunized Rabbits

(2018) Sabrina de Almeida Lima et al.   Frontiers in Immunology

Strategies for Generating Diverse Antibody Repertoires Using Transgenic Animals Expressing Human Antibodies

(2018) Weihsu C. Chen et al.   Frontiers in Immunology

Mouse DC-SIGN/CD209a as Target for Antigen Delivery and Adaptive Immunity

(2018) Sjoerd T. T. Schetters et al.   Frontiers in Immunology

Therapeutic Prospects of Extracellular Vesicles in Cancer Treatment

(2018) Daria S. Chulpanova et al.   Frontiers in Immunology

Virus-like particle display of HER2 induces potent anti-cancer responses

(2018) Arianna Palladini et al.   OncoImmunology

A New Anti-loxoscelic Serum Produced Against Recombinant Sphingomyelinase D: Results of Preclinical Trials

(2018) Daniel Manzoni de Almeida et al.   AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE

Integrating Engineering, Manufacturing, and Regulatory Considerations in the Development of Novel Antivenoms

(2018) Andreas Laustsen et al.   Toxins

Bioengineering of Bordetella pertussis Adenylate Cyclase Toxin for Antigen-Delivery and Immunotherapy

(2018) Alexandre Chenal et al.   Toxins

Antibody Cross-Reactivity in Antivenom Research

(2018) Line Ledsgaard et al.   Toxins

Strategies and industrial perspectives to improve oral absorption of biological macromolecules

(2017) Chang Liu et al.   Expert Opinion on Drug Delivery

Assessment of the neutralizing potency of antisera raised against native and γ-irradiated Naja nigricollis (black-necked spitting cobra) venom in rabbits, concerning its cardiotoxic effect

(2017) F Abdou et al.   HUMAN & EXPERIMENTAL TOXICOLOGY

Exploring the venom of the forest cobra snake: Toxicovenomics and antivenom profiling of Naja melanoleuca

(2017) Line P. Lauridsen et al.   Journal of Proteomics

Strategies in ‘snake venomics’ aiming at an integrative view of compositional, functional, and immunological characteristics of venoms

(2017) Bruno Lomonte et al.   JOURNAL OF VENOMOUS ANIMALS AND TOXINS INCLUDING TROPICAL DISEASES

Protein-species quantitative venomics: looking through a crystal ball

(2017) Juan J. Calvete et al.   JOURNAL OF VENOMOUS ANIMALS AND TOXINS INCLUDING TROPICAL DISEASES

Snake bite in India: a neglected disease of poverty

(2017) Himmatrao Saluba Bawaskar et al.   LANCET

Guiding recombinant antivenom development by omics technologies

(2017) Andreas H. Laustsen   New Biotechnology

BepiPred-2.0: improving sequence-based B-cell epitope prediction using conformational epitopes

(2017) Martin Closter Jespersen et al.   NUCLEIC ACIDS RESEARCH

Design and expression of recombinant toxins from Mexican scorpions of the genus Centruroides for production of antivenoms

(2017) J.M. Jiménez-Vargas et al.   TOXICON

High-density peptide microarray exploration of the antibody response in a rabbit immunized with a neurotoxic venom fraction

(2017) Mikael Engmark et al.   TOXICON

Comparison of the adjuvant activity of emulsions with different physicochemical properties on the antibody response towards the venom of West African carpet viper (Echis ocellatus)

(2017) Juan Manuel Valverde et al.   TOXICON

Improving the malaria transmission-blocking activity of a Plasmodium falciparum 48/45 based vaccine antigen by SpyTag/SpyCatcher mediated virus-like display

(2017) Susheel K. Singh et al.   VACCINE

A novel “in-feed” delivery platform applied for oral DNA vaccination against IPNV enables high protection in Atlantic salmon ( Salmon salar )

(2017) Miguel Reyes et al.   VACCINE

Cross-recognition of a pit viper (Crotalinae) polyspecific antivenom explored through high-density peptide microarray epitope mapping

(2017) Mikael Engmark et al.   PLoS Neglected Tropical Diseases

Recombinant snakebite antivenoms: A cost-competitive solution to a neglected tropical disease?

(2017) Andreas H. Laustsen et al.   PLoS Neglected Tropical Diseases

A Review and Database of Snake Venom Proteomes

(2017) Theo Tasoulis et al.   Toxins

Exploration of immunoglobulin transcriptomes from mice immunized with three-finger toxins and phospholipases A2from the Central American coral snake,Micrurus nigrocinctus

(2017) Andreas H. Laustsen et al.   PeerJ

Snakebite envenoming

(2017) José María Gutiérrez et al.   Nature Reviews Disease Primers

DNA-Loaded Cationic Liposomes Efficiently Function as a Vaccine against Malarial Proteins

(2017) Wesley L. Fotoran et al.   Molecular Therapy-Methods & Clinical Development

Efficient functional neutralization of lethal peptide toxins in vivo by oligonucleotides

(2017) Tarek Mohamed Abd El-Aziz et al.   Scientific Reports

Construction and expression of DNA vaccine against reddish body iridovirus and evaluation of immune efficacy in turbot (Scophthalmus maximus )

(2016) Fengrong Zheng et al.   AQUACULTURE RESEARCH

Engineering antibody therapeutics

(2016) Mark L Chiu et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

From Fangs to Pharmacology: The Future of Snakebite Envenoming Therapy

(2016) Andreas Laustsen et al.   CURRENT PHARMACEUTICAL DESIGN

Toxicovenomics and antivenom profiling of the Eastern green mamba snake ( Dendroaspis angusticeps )

(2016) Line P. Lauridsen et al.   Journal of Proteomics

Heterologous expression, protein folding and antibody recognition of a neurotoxin from the Mexican coral snake Micrurus laticorallis

(2016) Herlinda Clement et al.   JOURNAL OF VENOMOUS ANIMALS AND TOXINS INCLUDING TROPICAL DISEASES

Paths to the discovery of antivenom serotherapy in France

(2016) Rosany Bochner   JOURNAL OF VENOMOUS ANIMALS AND TOXINS INCLUDING TROPICAL DISEASES

Toxicity of crude and detoxifiedTityus serrulatusvenom in anti-venom-producing sheep

(2016) Marina G. Ferreira et al.   Journal of Veterinary Science

Snakebites: costing recombinant antivenoms

(2016) Andreas H. Laustsen   NATURE

Venoms of Micrurus coral snakes: Evolutionary trends in compositional patterns emerging from proteomic analyses

(2016) Bruno Lomonte et al.   TOXICON

Venom conjugated polylactide applied as biocompatible material for passive and active immunotherapy against scorpion envenomation

(2016) Sana Ayari-Riabi et al.   VACCINE

Protective antibodies against a sphingomyelinase D from Loxosceles intermedia spider venom elicited in mice with different genetic background

(2016) Camila Franco Batista Oliveira et al.   VACCINE

Development and characterization of a new carrier for vaccine delivery based on calcium-alginate nanoparticles: Safe immunoprotective approach against scorpion envenoming

(2016) Faez Amokrane Nait Mohamed et al.   VACCINE

A Heterologous Multiepitope DNA Prime/Recombinant Protein Boost Immunisation Strategy for the Development of an Antiserum against Micrurus corallinus (Coral Snake) Venom

(2016) Henrique Roman Ramos et al.   PLoS Neglected Tropical Diseases

A Comprehensive View of the Structural and Functional Alterations of Extracellular Matrix by Snake Venom Metalloproteinases (SVMPs): Novel Perspectives on the Pathophysiology of Envenoming

(2016) José Gutiérrez et al.   Toxins

Priority Actions and Progress to Substantially and Sustainably Reduce the Mortality, Morbidity and Socioeconomic Burden of Tropical Snakebite

(2016) Robert Harrison et al.   Toxins

Biotechnological Trends in Spider and Scorpion Antivenom Development

(2016) Andreas Laustsen et al.   Toxins

Bacterial superglue enables easy development of efficient virus-like particle based vaccines

(2016) Susan Thrane et al.   JOURNAL OF NANOBIOTECHNOLOGY

DNA immunization as a technology platform for monoclonal antibody induction

(2016) Shuying Liu et al.   Emerging Microbes & Infections

An Introduction to B-Cell Epitope Mapping and In Silico Epitope Prediction

(2016) Lenka Potocnakova et al.   Journal of Immunology Research

High-throughput immuno-profiling of mamba (Dendroaspis) venom toxin epitopes using high-density peptide microarrays

(2016) Mikael Engmark et al.   Scientific Reports

Nanotechnological carriers for cancer chemotherapy: The state of the art

(2015) Marilene Estanqueiro et al.   COLLOIDS AND SURFACES B-BIOINTERFACES

Advanced targeted therapies in cancer: Drug nanocarriers, the future of chemotherapy

(2015) Edgar Pérez-Herrero et al.   EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS

Accelerating antibody discovery using transgenic animals overexpressing the neonatal Fc receptor as a result of augmented humoral immunity

(2015) Judit Cervenak et al.   IMMUNOLOGICAL REVIEWS

Snapshots of scorpion venomics

(2015) Mohamed A. Abdel-Rahman et al.   JOURNAL OF ARID ENVIRONMENTS

An overview of bioinformatics tools for epitope prediction: Implications on vaccine development

(2015) Ruth E. Soria-Guerra et al.   JOURNAL OF BIOMEDICAL INFORMATICS

Unveiling the nature of black mamba (Dendroaspis polylepis) venom through venomics and antivenom immunoprofiling: Identification of key toxin targets for antivenom development

(2015) Andreas H. Laustsen et al.   Journal of Proteomics

A dendritic cell vaccine increases the breadth and diversity of melanoma neoantigen-specific T cells

(2015) B. M. Carreno et al.   SCIENCE

Anti-loxoscelic horse serum produced against a recombinant dermonecrotic protein of Brazilian Loxosceles intermedia spider neutralize lethal effects of Loxosceles laeta venom from Peru

(2015) C.G. Duarte et al.   TOXICON

Danger in the reef: Proteome, toxicity, and neutralization of the venom of the olive sea snake, Aipysurus laevis

(2015) Andreas H. Laustsen et al.   TOXICON

Evolution of alternative methodologies of scorpion antivenoms production

(2015) A.O. Carmo et al.   TOXICON

Lachesis stenophrys venom reduces the equine antibody response towards Bothrops asper venom used as co-immunogen in the production of polyspecific snake antivenom

(2015) Cynthia Arroyo et al.   TOXICON

Selecting key toxins for focused development of elapid snake antivenoms and inhibitors guided by a Toxicity Score

(2015) Andreas H. Laustsen et al.   TOXICON

A bright future for integrative venomics

(2015) Juan J. Calvete et al.   TOXICON

Immunoinformatics and epitope prediction in the age of genomic medicine

(2015) Linus Backert et al.   Genome Medicine

Predicting linear B-cell epitopes using amino acid anchoring pair composition

(2015) Weike Shen et al.   BioData Mining

Human Antibody Production in Transgenic Animals

(2014) Marianne Brüggemann et al.   ARCHIVUM IMMUNOLOGIAE ET THERAPIAE EXPERIMENTALIS

Bacterial Overexpression of Recombinant Heteroscorpine-1 (rHS-1), a Toxin from Heterometrus laoticus Scorpion Venom: Trends for Antibacterial Application and Antivenom Production

(2014) Nunthawun Uawonggul et al.   BIOCHEMICAL GENETICS

Improving B-cell epitope prediction and its application to global antibody-antigen docking

(2014) Konrad Krawczyk et al.   BIOINFORMATICS

Immunological profile of antivenoms: Preclinical analysis of the efficacy of a polyspecific antivenom through antivenomics and neutralization assays

(2014) José María Gutiérrez et al.   Journal of Proteomics

Venomous snakes of Costa Rica: Biological and medical implications of their venom proteomic profiles analyzed through the strategy of snake venomics

(2014) Bruno Lomonte et al.   Journal of Proteomics

Scorpion Envenomation

(2014) Geoffrey K. Isbister et al.   NEW ENGLAND JOURNAL OF MEDICINE

Innovative immunization protocols using chimeric recombinant protein for the production of polyspecific loxoscelic antivenom in horses

(2014) Luís F.M. Figueiredo et al.   TOXICON

Pharmacology of spider venom toxins

(2014) Devaraja Sannaningaiah et al.   Toxin Reviews

Generation and molecular characterization of a monoclonal antibody reactive with conserved epitope in sphingomyelinases D from Loxosceles spider venoms

(2014) C. Dias-Lopes et al.   VACCINE

A novel method for synthetic vaccine construction based on protein assembly

(2014) Zhida Liu et al.   Scientific Reports

Engineering Venom’s Toxin-Neutralizing Antibody Fragments and Its Therapeutic Potential

(2014) Larissa Alvarenga et al.   Toxins

Omics Meets Biology: Application to the Design and Preclinical Assessment of Antivenoms

(2014) Juan Calvete et al.   Toxins

Production of Recombinant Alpha Neurotoxin of Scorpion Venom Mesobuthus eupeus and Analysis of its Immunogenicity

(2014) Ghafar Eskandari et al.   Iranian Red Crescent Medical Journal

Cloning, expression and characterization of a phospholipase D from Loxosceles gaucho venom gland

(2013) Geraldo S. Magalhães et al.   BIOCHIMIE

Bioinformatics Resources and Tools for Conformational B-Cell Epitope Prediction

(2013) Pingping Sun et al.   Computational and Mathematical Methods in Medicine

Integrated Analysis of Transcriptomic and Proteomic Data

(2013) Saad Haider et al.   CURRENT GENOMICS

Comparison between two methods of scorpion venom milking in Morocco

(2013) Naoual Oukkache et al.   JOURNAL OF VENOMOUS ANIMALS AND TOXINS INCLUDING TROPICAL DISEASES

Improved Method for Linear B-Cell Epitope Prediction Using Antigen’s Primary Sequence

(2013) Harinder Singh et al.   PLoS One

Snake venomics: From the inventory of toxins to biology

(2013) Juan J. Calvete   TOXICON

Scorpion venom components that affect ion-channels function

(2013) V. Quintero-Hernández et al.   TOXICON

Generation and characterization of a recombinant chimeric protein (rCpLi) consisting of B-cell epitopes of a dermonecrotic protein from Loxosceles intermedia spider venom

(2013) T.M. Mendes et al.   VACCINE

Fusion protein linkers: Property, design and functionality

(2012) Xiaoying Chen et al.   ADVANCED DRUG DELIVERY REVIEWS

Assessment of snake antivenom purity by comparing physicochemical and immunochemical methods

(2012) Álvaro Segura et al.   BIOLOGICALS

Maintaining Coral Snakes (Micrurus nigrocinctus, Serpentes: Elapidae) for venom production on an alternative fish-based diet

(2012) Danilo Chacón et al.   TOXICON

Serum production against Tityus serrulatus scorpion venom using cross-linked chitosan nanoparticles as immunoadjuvant

(2012) Karla S. Rocha Soares et al.   TOXICON

Second generation snake antivenomics: Comparing immunoaffinity and immunodepletion protocols

(2012) Davinia Pla et al.   TOXICON

The long road of research on snake venom serine proteinases

(2012) Solange M.T. Serrano   TOXICON

Snake venom metalloproteinases

(2012) Francis S. Markland et al.   TOXICON

Reliable B Cell Epitope Predictions: Impacts of Method Development and Improved Benchmarking

(2012) Jens Vindahl Kringelum et al.   PLoS Computational Biology

Antivenoms for the treatment of snakebite envenomings: The road ahead

(2011) José María Gutiérrez et al.   BIOLOGICALS

Proteomic tools against the neglected pathology of snake bite envenoming

(2011) Juan J Calvete   Expert Review of Proteomics

Venomic and Antivenomic Analyses of the Central American Coral Snake,Micrurus nigrocinctus(Elapidae)

(2011) Julián Fernández et al.   JOURNAL OF PROTEOME RESEARCH

Ending the drought: New strategies for improving the flow of affordable, effective antivenoms in Asia and Africa

(2011) David J. Williams et al.   Journal of Proteomics

Spider bite

(2011) Geoffrey K Isbister et al.   LANCET

Mimotopes of mutalysin-II from Lachesis muta snake venom induce hemorrhage inhibitory antibodies upon vaccination of rabbits

(2011) R.A. Machado de Avila et al.   PEPTIDES

Protection against the toxic effects of Loxosceles intermedia spider venom elicited by mimotope peptides

(2011) J. de Moura et al.   VACCINE

Phospholipase-D activity and inflammatory response induced by brown spider dermonecrotic toxin: Endothelial cell membrane phospholipids as targets for toxicity

(2010) Olga M. Chaim et al.   BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS

Loxoscelism: From basic research to the proposal of new therapies

(2010) Denise V. Tambourgi et al.   TOXICON

Antivenom: The most cost-effective treatment in the world?

(2010) N. Brown et al.   TOXICON

Structure, function and evolution of three-finger toxins: Mini proteins with multiple targets

(2010) R. Manjunatha Kini et al.   TOXICON

Epitopia: a web-server for predicting B-cell epitopes

(2009) Nimrod D Rubinstein et al.   BMC BIOINFORMATICS

Transcriptomic basis for an antiserum against Micrurus corallinus (coral snake) venom

(2009) Luciana I Leão et al.   BMC GENOMICS

Structure-Activity Relationships in Peptide-Antibody Complexes: Implications for Epitope Prediction and Development of Synthetic Peptide Vaccines

(2009) Shu-wen Chen et al.   CURRENT MEDICINAL CHEMISTRY

Venoms, venomics, antivenomics

(2009) Juan J. Calvete et al.   FEBS LETTERS

Heterologous expressed toxic and non-toxic peptide variants of toxin CssII are capable to produce neutralizing antibodies against the venom of the scorpion Centruroides suffusus suffusus

(2009) Kenya Hernández-Salgado et al.   IMMUNOLOGY LETTERS

Immunization with a synthetic robustoxin derivative lacking disulphide bridges protects against a potentially lethal challenge with funnel-web spider (Atrax robustus) venom

(2009) Alfio Comis et al.   JOURNAL OF BIOSCIENCES

Impact of Regional Variation inBothrops asperSnake Venom on the Design of Antivenoms: Integrating Antivenomics and Neutralization Approaches

(2009) José María Gutiérrez et al.   JOURNAL OF PROTEOME RESEARCH

Snake venomics and antivenomics: Proteomic tools in the design and control of antivenoms for the treatment of snakebite envenoming

(2009) José María Gutiérrez et al.   Journal of Proteomics

Heterologous expression of a gene that codes for Pg8, a scorpion toxin of Parabuthus granulatus, capable of generating protecting antibodies in mice

(2009) Blanca I. García-Gómez et al.   TOXICON

A protective immune response against lethal, dermonecrotic and hemorrhagic effects of Loxosceles intermedia venom elicited by a 27-residue peptide

(2009) C. Dias-Lopes et al.   TOXICON

An in vivo protective response against toxic effects of the dermonecrotic protein from Loxoscelesintermedia spider venom elicited by synthetic epitopes

(2009) Liza Felicori et al.   VACCINE

In vivo protection against Tityus serrulatus scorpion venom by antibodies raised against a discontinuous synthetic epitope

(2009) Clara Guerra Duarte et al.   VACCINE

Snake Envenoming: A Disease of Poverty

(2009) Robert A. Harrison et al.   PLoS Neglected Tropical Diseases

Epidemiology of scorpionism: A global appraisal

(2008) J.-P. Chippaux et al.   ACTA TROPICA

ElliPro: a new structure-based tool for the prediction of antibody epitopes

(2008) Julia Ponomarenko et al.   BMC BIOINFORMATICS

Cellular pathology induced by snake venom phospholipase A2 myotoxins and neurotoxins: common aspects of their mechanisms of action

(2008) C. Montecucco et al.   CELLULAR AND MOLECULAR LIFE SCIENCES

Predicting linear B‐cell epitopes using string kernels

(2008) Yasser EL‐Manzalawy et al.   JOURNAL OF MOLECULAR RECOGNITION

Snake Venomics and Antivenomics of the Arboreal Neotropical Pitvipers Bothriechis lateralis and Bothriechis schlegelii

(2008) Bruno Lomonte et al.   JOURNAL OF PROTEOME RESEARCH

An introduction to epitope prediction methods and software

(2008) Xingdong Yang et al.   REVIEWS IN MEDICAL VIROLOGY

Immunization with cDNA of a novel P-III type metalloproteinase from the rattlesnake Crotalus durissus durissus elicits antibodies which neutralize 69% of the hemorrhage induced by the whole venom

(2008) Gabriela Azofeifa-Cordero et al.   TOXICON

Effective Tityus serrulatus anti-venom produced using the Ts1 component

(2008) T.M. Mendes et al.   TOXICON

Peptide mimicking antigenic and immunogenic epitope of neuwiedase from Bothrops neuwiedi snake venom

(2008) R. Cardoso et al.   TOXICON

Neutralization of venom-induced hemorrhage by equine antibodies raised by immunization with a plasmid encoding a novel P-II metalloproteinase from the lancehead pitviper Bothrops asper

(2008) Viviana Arce-Estrada et al.   VACCINE

The Global Burden of Snakebite: A Literature Analysis and Modelling Based on Regional Estimates of Envenoming and Deaths

(2008) Anuradhani Kasturiratne et al.   PLOS MEDICINE