Article
Biochemistry & Molecular Biology
Mao-Ling Sun, Hai-Yun Lai, Na-Yu Chong, Dong-Fan Liu, Zhen-Yi Zhang, Bo Pang, Jun Yao
Summary: A new method for detecting HBV using a combination of MIRA and LFD technologies has been developed, which is simple, rapid, and accurate, making it particularly suitable for resource-limited regions.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2021)
Review
Food Science & Technology
Jie Zhou, Teng-Yu Wang, Zhi Lan, Han-Jie Yang, Xing-Jian Ye, Rui Min, Zhao-Hui Wang, Qing Huang, Jing Cao, Yu-E Gao, Wen-Long Wang, Xiu-Lan Sun, Yi Zhang
Summary: Foodborne microbial contamination (FMC) is the primary cause of food poisoning and illness. Isothermal amplification methods with functional nucleic acids (FNAs) have enabled the detection of mycotoxins, making biosensors for FMC more sensitive, cost-effective, and less reliant on nanomaterials for signal output.
FOOD RESEARCH INTERNATIONAL
(2023)
Article
Chemistry, Analytical
Hongbo Shan, Yang Wang, Tao Wu, Binwu Ying, Gaolian Xu
Summary: Asymmetric hairpins mediated amplification (HMA) is a novel isothermal amplification method developed for the diagnosis of Mycobacterium Tuberculosis (MTB). It has high sensitivity, specificity, and direct visualization, making it suitable for point-of-care (POC) diagnosis.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Analytical
Wenna Li, Weipan Peng, Yating Zhang, Ping Liu, Xiaoqun Gong, Hao Liu, Jin Chang
Summary: Oral squamous cell carcinoma (OSCC) is a well-known malignancy that poses a serious threat to human health. This study developed a biosensor platform based on the cascade nucleic acid amplification technology for colorimetric analysis of miRNA 31, which is associated with OSCC. The platform demonstrated high sensitivity and specificity for miRNA 31 detection.
ANALYTICA CHIMICA ACTA
(2022)
Review
Chemistry, Analytical
Poramin Boonbanjong, Kiatnida Treerattrakoon, Wassa Waiwinya, Piyawat Pitikultham, Deanpen Japrung
Summary: Isothermal amplification (IA) is a nucleic acid amplification technology that has significantly contributed to the healthcare system by improving disease diagnosis through higher sensitivity, specificity, and rapidity. Certain IA techniques provide a rapid, sensitive, specific, and simple method for disease diagnosis. However, not all IA techniques have been widely used in clinical applications due to the influence of different biomarkers and sample types on the IA system.
Review
Plant Sciences
Aleksandr Ivanov, Irina Safenkova, Anatoly Zherdev, Boris B. Dzantiev
Summary: Rapid, sensitive, and timely diagnostics are crucial for protecting plants from pathogens, with isothermal amplification methods providing a feasible solution for in-field molecular diagnostics. Combining isothermal amplification techniques with extraction and detection methods compatible with outdoor phytodiagnostics is essential for preventing and controlling the spread of disease caused by viral, bacterial, and fungal phytopathogens. The development of rapid, sensitive, and equipment-free nucleic acid detection methods is seen as the future of phytodiagnostics, showcasing visible benefits.
Article
Biotechnology & Applied Microbiology
Xiaohan Yang, Panpan Zhao, Yu Dong, Shiqi Chen, Hui Shen, Ge Jiang, Hai Zhu, Jingquan Dong, Song Gao
Summary: A new rapid detection method for V. vulnificus was developed using RPA technology and LFS strip, with good specificity and sensitivity, suitable for on-site detection.
Article
Chemistry, Analytical
Shengjun Bu, Qianyu Zhou, Liming Liu, Chunyang Zhou, Zhuo Hao, Jiayu Wan, Chunying Pang
Summary: The present study describes a novel technology called TPIA that enables target nucleic acid detection. By using specific design strategy and fluorescence techniques, this system provides a versatile tool with great potential for biomedical research and clinical diagnosis.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Immunology
Duobao Ge, Fang Wang, Yanyan Hu, Bendi Wang, Xuzhu Gao, Zhenxing Chen
Summary: This study combined isothermal recombinase polymerase amplification (RPA) with nanoparticle-based lateral flow strips (LFS) to establish a method for the rapid detection of Porphyromonas gingivalis. The method showed high specificity and sensitivity, with a completion time of 30 minutes and visual detection of the amplification products. It simplifies the tedious process of detecting P. gingivalis.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Biophysics
Ting Zheng, Xianming Li, Yanjun Si, Minjin Wang, Yuzhen Zhou, Yusheng Yang, Na Liang, Binwu Ying, Peng Wu
Summary: In this study, an accurate point-of-care testing (POCT) platform was proposed by specific recognition of target amplicons with peptide nucleic acid (PNA, assisted by T7 Exonuclease), which efficiently eliminated false positives and showed high sensitivity and specificity for detecting SARS-CoV-2.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Multidisciplinary Sciences
Yujin Jung, Jayeon Song, Hyun Gyu Park
Summary: The PHAmp method described in this study utilizes a phosphorothioated hairpin probe to assist in isothermal amplification, allowing for the detection of target nucleic acid down to 0.29 fM with a wide dynamic range and excellent specificity. The unique design principle may provide insights for the development of novel isothermal amplification methods for nucleic acid detection.
SCIENTIFIC REPORTS
(2021)
Article
Chemistry, Analytical
Ting Zheng, Xianming Li, Ya-Ni Xie, Bin Yang, Peng Wu
Summary: This study proposes a dual-gene RPA-LFA method to selectively detect E. coli O157:H7 by recognizing target amplicons and eliminating false positives. The assay shows high sensitivity and specificity for the detection of this pathogen.
ANALYTICAL CHEMISTRY
(2023)
Article
Biophysics
Yong Sun, Panzhu Qin, Jun He, Weiwei Li, Yonglin Shi, Jianguo Xu, Qian Wu, Qingqing Chen, Weidong Li, Xinxin Wang, Guodong Liu, Wei Chen
Summary: This work introduces a new detection technology that can simultaneously test for SARS-CoV-2 and influenza viruses, with advantages such as speed, high sensitivity, no cross-reactivity, and false positives. Its clinical application demonstrates high accuracy, and this portable device is expected to meet the global healthcare system's need for online monitoring of serious infectious diseases.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Chemistry, Analytical
Hao Jiang, Yuan Li, Xuefei Lv, Yulin Deng, Xiaoqiong Li
Summary: Nucleic acid amplification techniques, such as polymerase chain reaction (PCR) and isothermal amplification, are important for nucleic acid detection. However, PCR is limited by temperature control and isothermal amplification has its own limitations. Efforts to integrate enzymes and amplification techniques have the potential to overcome these limitations. This review provides a comprehensive summary of cascade amplification, including design fundamentals, signal generation, evolution, applications, challenges, and trends.
Article
Chemistry, Analytical
Xiuen Cao, Chuanpin Chen, Qubo Zhu
Summary: This review summarizes the applications of functional nucleic acids and isothermal amplification techniques in biosensing, highlighting their important contributions to the field. These technologies have great potential to enhance the performance of biosensors.
Article
Food Science & Technology
David Steiner, Armin Humpel, Eleonore Stamminger, Anna Schoeberl, Gerlinde Pachschwoell, Anita Sloboda, Christy Swoboda, Jolene Rigg, Dawei Zhang, Yahong Wang, Joshua Davis, Michael Sulyok, Rudolf Krska, Brian Quinn, Brett Greer, Christopher T. Elliott, Zbynek Dzuman, Jana Hajslova, Andreas Gschaider, Carina Fechner, Lisa Forstner, Elisabeth Varga, Piotr Jedziniak, Katarzyna Pietruszka, Adrianna Rudawska, Alexandra Malachova
Summary: This interlaboratory comparison study involved nine laboratories from around the world testing for 24 regulated and non-regulated mycotoxins. The overall success rate for testing all compounds was 70%. The study also showed that the design is suitable for providing future certified reference materials.
Article
Food Science & Technology
Felipe Penagos-Tabares, Ratchaneewan Khiaosa-ard, Marlene Schmidt, Eva-Maria Bartl, Johanna Kehrer, Veronika Nagl, Johannes Faas, Michael Sulyok, Rudolf Krska, Qendrim Zebeli
Summary: Dairy cows in Austria are commonly exposed to a mixture of mycotoxins and phytoestrogens in their diets, with Fusarium-derived metabolites being the most prevalent and diverse. Maize silage and straw are the major dietary factors influencing the contamination with fungal toxins and metabolites, while temperature plays a significant role among the geo-climatic factors.
Article
Food Science & Technology
Valentina Scarpino, Michael Sulyok, Rudolf Krska, Amedeo Reyneri, Massimo Blandino
Summary: This study investigated the role of soil fertility resulting from different nitrogen fertilization rates on the contamination of mycotoxins and fungal metabolites in maize grains. The results showed that poor soil fertility was associated with increased contamination of certain fungal metabolites, while nitrogen overload led to higher contamination of other fungal metabolites. A balanced use of nitrogen fertilizer was found to be the best solution to reduce mycotoxin and fungal metabolite contamination.
Article
Food Science & Technology
Kolawole I. Ayeni, Michael Sulyok, Rudolf Krska, Benedikt Warth, Chibundu N. Ezekiel
Summary: This study evaluated the presence of mycotoxins and other contaminants in complementary foods consumed by Nigerian infants and young children. The results showed that some foods exceeded the EU safety threshold for aflatoxin and fumonisin. Other mycotoxins were also detected. The study emphasizes the importance of storage and hygiene practices during food preparation and feeding.
Article
Chemistry, Applied
Elzbieta Suchowilska, Wolfgang Kandler, Marian Wiwart, Rudolf Krska
Summary: Tritordeum grain has lower thousand kernel weight and ash content compared to durum wheat and barley, but it contains higher levels of potassium, magnesium, copper, zinc, and cobalt.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2023)
Article
Environmental Sciences
Julia Derx, Rita Linke, Silvia Cervero-Arago, Christina Frick, Jack Schijven, Alexander K. T. Kirschner, Gerhard Lindner, Julia Walochnik, Gabrielle Stalder, Regina Sommer, Ernis Saracevic, Matthias Zessner, Alfred P. Blaschke, Andreas H. Farnleitner, H. Seda
Summary: We developed an innovative approach to estimate fecal pollution sources in urban river catchments. The approach involved catchment surveys, hypothesis generation, and verification with measured concentrations and correlation analysis. The results showed that treated wastewater discharge was the primary contributor to fecal pollution in dry weather, while combined sewer overflows were the main source in wet weather. The approach also demonstrated statistical relationships between microbial tracers and genetic fecal markers in the river, facilitating water safety management and microbial infection risk assessment.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Entomology
Hanano Yamada, Benewende Aristide Kabore, Nanwintoum Severin Bimbile Somda, Nonhlanhla L. L. Ntoyi, Chantel Janet de Beer, Jeremy Bouyer, Carlos Caceres, Robert L. L. Mach, Yeudiel Gomez-Simuta
Summary: The sterile insect technique (SIT) is an environmentally friendly and species-specific method of pest control that involves mass production of target insects in a factory and sterilizing them using irradiation, typically gamma rays. As gamma sources become more difficult and expensive to obtain, and regulations surrounding their use become stricter, there is growing interest in alternative irradiation methods such as X-rays. A recent study found that X-rays can be used effectively to sterilize major SIT target insect species, making X-ray units suitable for small-to-medium-sized SIT programs.
Review
Microbiology
Katalin Demeter, Rita Linke, Elisenda Balleste, Georg Reischer, Rene E. Mayer, Julia Vierheilig, Claudia Kolm, Margaret E. Stevenson, Julia Derx, Alexander K. T. Kirschner, Regina Sommer, Orin C. Shanks, Anicet R. Blanch, Joan B. Rose, Warish Ahmed, Andreas H. Farnleitner
Summary: The proposed discipline of genetic faecal pollution diagnostics helps address complex water, sanitation, and human health-related issues. This systematic review elucidates application areas, key research questions, and study designs from over 1100 scientific articles and provides critical insights and conclusions.
FEMS MICROBIOLOGY REVIEWS
(2023)
Article
Food Science & Technology
Felipe Penagos-Tabares, Michael Sulyok, Juan-Ignacio Artavia, Samanta-Irais Flores-Quiroz, Cesar Garzon-Perez, Ezequias Castillo-Lopez, Luis Zavala, Juan-David Orozco, Johannes Faas, Rudolf Krska, Qendrim Zebeli
Summary: This study analyzed a wide range of mycotoxins, phytoestrogens, and other metabolites in dairy feeds from Mexican farms, and found widespread contamination. Fusarium-derived secondary metabolites were the most frequently detected fungal compounds. The most commonly detected mycotoxins in the feeds were zearalenone, fumonisin B1, and deoxynivalenol.
Article
Agronomy
Urszula Wachowska, Michael Sulyok, Marian Wiwart, Elzbieta Suchowilska, Weronika Giedrojc, Dariusz Gontarz, Wolfgang Kandler, Rudolf Krska
Summary: The efficacy of Debaryomyces hansenii (Dh) yeast in reducing the content of secondary fungal metabolites in durum wheat spikes grown in southern and northern Poland was evaluated. The results showed that the Dh yeast strain significantly decreased mycotoxin concentration in the grain and also reduced the content of other fungal metabolites in northern Poland.
Article
Food Science & Technology
Oluwadamilola M. M. Makinde, Michael Sulyok, Rasheed A. A. Adeleke, Rudolf Krska, Chibundu N. N. Ezekiel
Summary: A comprehensive study was conducted to analyze bacterial and biotoxin contaminants in ready-to-eat (RTE) foods in Nigeria. 16S rRNA gene sequencing and dilute-and-shoot LC-MS/MS method were used to profile bacteria and biotoxins, respectively. The study discovered new bacterial isolates and detected various metabolites in the RTE foods. It also found different types of contamination in artisanal and industrially-processed foods, highlighting the need for routine surveillance and food safety education.
Review
Food Science & Technology
Michael Wiederstein, Sabine Baumgartner, Kathrin Lauter
Summary: Soybean is a widely used vegetarian protein source, but it can also cause allergies in a small percentage of individuals. This review discusses the classification of major and minor soybean allergens, methods for detecting allergens, the impact of food processing on soybean allergenicity, and strategies for managing allergen risks to protect soybean-allergic patients.
ACS FOOD SCIENCE & TECHNOLOGY
(2023)
Review
Medicine, General & Internal
Carmen Rehm, Claudia Kolm, Sonja Pleininger, Florian Heger, Alexander Indra, Georg H. Reischer, Andreas A. H. Farnleitner, Alexander K. T. Kirschner
Summary: This review summarizes the knowledge gained in the past two decades on Vibrio cholerae infections and its occurrence in bathing waters in Austria, specifically focusing on lake Neusiedler See. It discusses the increase in non-toxigenic, non-O1/non-O139 V. cholerae (NTVC) infections and the specific environmental conditions that favor their occurrence. The review also provides an overview of current state-of-the-art methods for clinical and environmental diagnostics and presents a preliminary public health risk assessment for NTVC infections related to Neusiedler See. Furthermore, it discusses typical symptoms, possible treatment options, and the antibiotic resistance status of Austrian NTVC isolates to raise awareness among healthcare professionals.
WIENER KLINISCHE WOCHENSCHRIFT
(2023)
Review
Food Science & Technology
Michael Wiederstein, Sabine Baumgartner, Kathrin Lauter
Summary: Soybean, as a widely used vegetarian protein source, has both nutritional benefits and allergenic properties. Soybean allergy affects a small percentage of the population worldwide and can have severe consequences. However, there is still no consensus on the classification, severity, risk assessment, and labeling requirements for soybean allergens.
ACS FOOD SCIENCE & TECHNOLOGY
(2023)