4.7 Article

Cocktails of Mycotoxins, Phytoestrogens, and Other Secondary Metabolites in Diets of Dairy Cows in Austria: Inferences from Diet Composition and Geo-Climatic Factors

Journal

TOXINS
Volume 14, Issue 7, Pages -

Publisher

MDPI
DOI: 10.3390/toxins14070493

Keywords

mycotoxin; phytoestrogen; ergot alkaloid; co-exposure; dairy farming; feed safety

Funding

  1. Austrian Federal Ministry for Climate Action, Environment, Energy, Mobility, Innovation and Technology (BMK)
  2. Austrian Federal Ministry for Digital and Economic Affairs (BMDW)
  3. Austrian Research Promotion Agency (FFG)

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Dairy cows in Austria are commonly exposed to a mixture of mycotoxins and phytoestrogens in their diets, with Fusarium-derived metabolites being the most prevalent and diverse. Maize silage and straw are the major dietary factors influencing the contamination with fungal toxins and metabolites, while temperature plays a significant role among the geo-climatic factors.
Dairy production is a pivotal economic sector of Austrian and European agriculture. Dietary toxins and endocrine disruptors of natural origin such as mycotoxins and phytoestrogens can affect animal health, reproduction, and productivity. This study characterized the profile of a wide spectrum of fungal, plant, and unspecific secondary metabolites, including regulated, emerging, and modified mycotoxins, phytoestrogens, and cyanogenic glucosides, in complete diets of lactating cows from 100 Austrian dairy farms. To achieve this, a validated multi-metabolite liquid chromatography/electrospray ionization-tandem mass spectrometric (LC/ESI-MS/MS) method was employed, detecting 155 of >800 tested metabolites. Additionally, the most influential dietary and geo-climatic factors related to the dietary mycotoxin contamination of Austrian dairy cattle were recognized. We evidenced that the diets of Austrian dairy cows presented ubiquitous contamination with mixtures of mycotoxins and phytoestrogens. Metabolites derived from Fusarium spp. presented the highest concentrations, were the most recurrent, and had the highest diversity among the detected fungal compounds. Zearalenone, deoxynivalenol, and fumonisin B1 were the most frequently occurring mycotoxins considered in the EU legislation, with detection frequencies >70%. Among the investigated dietary factors, inclusion of maize silage (MS) and straw in the diets was the most influential factor in contamination with Fusarium-derived and other fungal toxins and metabolites, and temperature was the most influential among the geo-climatic factors.

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