Cryo–electron microscopy structure of the lipid droplet–formation protein seipin

Mechanism and Determinants of Amphipathic Helix-Containing Protein Targeting to Lipid Droplets

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The assembly of lipid droplets and their roles in challenged cells

(2018) W Mike Henne et al.   EMBO JOURNAL

Rab18 is not necessary for lipid droplet biogenesis or turnover in human mammary carcinoma cells

(2018) Christina B. K. Jayson et al.   MOLECULAR BIOLOGY OF THE CELL

Lipid Droplets in Health and Disease

(2017) Gizem Onal et al.   Lipids in Health and Disease

Organelle biogenesis in the endoplasmic reticulum

(2017) Amit S. Joshi et al.   NATURE CELL BIOLOGY

RosettaES: a sampling strategy enabling automated interpretation of difficult cryo-EM maps

(2017) Brandon Frenz et al.   NATURE METHODS

MotionCor2: anisotropic correction of beam-induced motion for improved cryo-electron microscopy

(2017) Shawn Q Zheng et al.   NATURE METHODS

Seipin regulates ER–lipid droplet contacts and cargo delivery

(2016) Veijo T Salo et al.   EMBO JOURNAL

Cryo-EM structure of lysenin pore elucidates membrane insertion by an aerolysin family protein

(2016) Monika Bokori-Brown et al.   Nature Communications

SEIPIN Regulates Lipid Droplet Expansion and Adipocyte Development by Modulating the Activity of Glycerol-3-phosphate Acyltransferase

(2016) Martin Pagac et al.   Cell Reports

Seipin is required for converting nascent to mature lipid droplets

(2016) Huajin Wang et al.   eLife

Seipin is involved in the regulation of phosphatidic acid metabolism at a subdomain of the nuclear envelope in yeast

(2015) Heimo Wolinski et al.   BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS

Dissecting seipin function: the localized accumulation of phosphatidic acid at ER/LD junctions in the absence of seipin is suppressed by Sei1pΔNterm only in combination with Ldb16p

(2015) Sungwon Han et al.   BMC CELL BIOLOGY

Molecular Mechanism of V(D)J Recombination from Synaptic RAG1-RAG2 Complex Structures

(2015) Heng Ru et al.   CELL

Protein Crowding Is a Determinant of Lipid Droplet Protein Composition

(2015) Nora Kory et al.   DEVELOPMENTAL CELL

The seipin complex Fld1/Ldb16 stabilizes ER–lipid droplet contact sites

(2015) Alexandra Grippa et al.   JOURNAL OF CELL BIOLOGY

CTFFIND4: Fast and accurate defocus estimation from electron micrographs

(2015) Alexis Rohou et al.   JOURNAL OF STRUCTURAL BIOLOGY

De novo protein structure determination from near-atomic-resolution cryo-EM maps

(2015) Ray Yu-Ruei Wang et al.   NATURE METHODS

Seipin oligomers can interact directly with AGPAT2 and lipin 1, physically scaffolding critical regulators of adipogenesis

(2015) Md. Mesbah Uddin Talukder et al.   Molecular Metabolism

Seipin Promotes Adipose Tissue Fat Storage through the ER Ca2+-ATPase SERCA

(2014) Junfeng Bi et al.   Cell Metabolism

Single particle electron cryo-microscopy of a mammalian ion channel

(2014) Maofu Liao et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Control of lipid droplet size in budding yeast requires the collaboration between Fld1 and Ldb16

(2014) C.-W. Wang et al.   JOURNAL OF CELL SCIENCE

Screening and large-scale expression of membrane proteins in mammalian cells for structural studies

(2014) April Goehring et al.   Nature Protocols

Niemann-Pick type C2 protein mediating chemical communication in the worker ant

(2014) Y. Ishida et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Advances in Analysis of Low Signal-to-Noise Images Link Dynamin and AP2 to the Functions of an Endocytic Checkpoint

(2013) François Aguet et al.   DEVELOPMENTAL CELL

Alleviation of seipinopathy-related ER stress by triglyceride storage

(2012) M. Holtta-Vuori et al.   HUMAN MOLECULAR GENETICS

RELION: Implementation of a Bayesian approach to cryo-EM structure determination

(2012) Sjors H.W. Scheres   JOURNAL OF STRUCTURAL BIOLOGY

Protein structure prediction from sequence variation

(2012) Debora S Marks et al.   NATURE BIOTECHNOLOGY

Fiji: an open-source platform for biological-image analysis

(2012) Johannes Schindelin et al.   NATURE METHODS

The human lipodystrophy protein seipin is an ER membrane adaptor for the adipogenic PA phosphatase lipin 1

(2012) M.F. Michelle Sim et al.   Molecular Metabolism

A role for seipin in lipid droplet dynamics and inheritance in yeast

(2011) H. Wolinski et al.   JOURNAL OF CELL SCIENCE

A Bayesian View on Cryo-EM Structure Determination

(2011) Sjors H.W. Scheres   JOURNAL OF MOLECULAR BIOLOGY

Protein 3D Structure Computed from Evolutionary Sequence Variation

(2011) Debora S. Marks et al.   PLoS One

A Role for Phosphatidic Acid in the Formation of “Supersized” Lipid Droplets

(2011) Weihua Fei et al.   PLoS Genetics

Visualizing Proteins and Macromolecular Complexes by Negative Stain EM: from Grid Preparation to Image Acquisition

(2011) David S. Booth et al.   Jove-Journal of Visualized Experiments

PHENIX: a comprehensive Python-based system for macromolecular structure solution

(2010) Paul D. Adams et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

Seipin Is a Discrete Homooligomer

(2010) Derk Binns et al.   BIOCHEMISTRY

Identification of Surface Residues on Niemann-Pick C2 Essential for Hydrophobic Handoff of Cholesterol to NPC1 in Lysosomes

(2010) Michael L. Wang et al.   Cell Metabolism

Quantitative analysis of cryo-EM density map segmentation by watershed and scale-space filtering, and fitting of structures by alignment to regions

(2010) Grigore D. Pintilie et al.   JOURNAL OF STRUCTURAL BIOLOGY

MolProbity: all-atom structure validation for macromolecular crystallography

(2009) Vincent B. Chen et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

HELIQUEST: a web server to screen sequences with specific  -helical properties

(2008) R. Gautier et al.   BIOINFORMATICS

Fld1p, a functional homologue of human seipin, regulates the size of lipid droplets in yeast

(2008) Weihua Fei et al.   JOURNAL OF CELL BIOLOGY