Article
Biochemistry & Molecular Biology
Alex A. Meier, Krzysztof Kuczera, Minae Mure
Summary: This study generated a 3D-predicted structure of the C-terminal amine oxidase domain of LOXL2 and discussed the active site environment surrounding LTQ and Cu2+.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Multidisciplinary
Gokhan Bahcecioglu, Xiaoshan Yue, Erin Howe, Ian Guldner, M. Sharon Stack, Harikrishna Nakshatri, Siyuan Zhang, Pinar Zorlutuna
Summary: The study reveals that aging ECM can drive normal mammary epithelial cells to a more invasive and cancer-like phenotype, and enhance motility and invasiveness in breast cancer cells. Different responses of cells to young and aged ECMs may lead to the identification of new targets for cancer treatment and prevention.
Article
Biochemistry & Molecular Biology
Alex A. Meier, Hee-Jung Moon, Ronald Toth, Ewa Folta-Stogniew, Krzysztof Kuczera, C. Russell Middaugh, Minae Mure
Summary: LOXL2 is a promising therapeutic target against metastatic/invasive tumors and organ and tissue fibrosis by promoting crosslinking of proteins in the ECM. The study found that while fl-LOXL2 predominantly exists as a monomer, Delta 1-2SRCR-LOXL2 exists solely as a monomer.
Article
Biology
Huilei Wang, Alan Poe, Marta Martinez Yus, Lydia Pak, Kavitha Nandakumar, Lakshmi Santhanam
Summary: This study identifies Factor Xa as a protease involved in the processing of LOXL2, which changes its affinity and substrate preference. Processing by Factor Xa leads to decreased cross-linking activity in the ECM and increased interactions with LOX, indicating a potential compensatory mechanism. This finding has significant implications in diseases where LOXL2 is involved.
COMMUNICATIONS BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Alex A. Meier, Eden P. Go, Hee-Jung Moon, Heather Desaire, Minae Mure
Summary: This study conducted a mass spectrometry-based disulfide mapping analysis to explore the disulfide bond structure of recombinant LOXL2. The results revealed a conserved set of five disulfide bonds in both the precursor and mature forms of LOXL2, while another set of four alternative disulfide bonds, detected in low abundance, was not associated with mature LOXL2.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Oncology
Sylvain D. Vallet, Coline Berthollier, Romain Salza, Laurent Muller, Sylvie Ricard-Blum
Summary: The study aimed to build and analyze the interaction network of the LOX family members, identifying rewiring in cancer. Computational analyses revealed the network's involvement in various cellular processes and its functional switch in colorectal carcinoma.
Article
Oncology
Han Young Kim, Mihee Jo, Ju A. La, Youngjin Choi, Eun Chul Cho, Su Hee Kim, Youngmee Jung, Kwangmeyung Kim, Ju Hee Ryu
Summary: This study demonstrates sensitive detection of LOX levels in tumor microenvironments using peptide-functionalized gold nanoprobes. The method shows high specificity to LOX and does not exhibit color changes in the presence of other enzymes. The developed LS-AuNPs can be used for predicting tumor stiffness and anticancer drug resistance based on LOX levels.
Article
Biochemistry & Molecular Biology
Nataliya Migulina, Gavin Tjin, Alen Faiz, Theo Borghuis, Fenghua Zhao, Hans J. Kaper, Marit Metzlar, Eline van Dijk, Prashant K. Sharma, Wim Timens, Reinoud Gosens, Corry-Anke Brandsma, Janette K. Burgess
Summary: This study investigated the effects of smoking and COPD on LOs gene and protein expression in the airways and found that LOs alterations may be related to smoking and contribute to impaired airway function in COPD, providing potential novel targets for prevention or treatment of small airways changes in COPD.
Article
Multidisciplinary Sciences
Anita Senk, Valentin Djonov
Summary: Despite being well investigated, the exact role of collagen fibers in supporting angiogenesis remains unclear. This study shows that collagen fibers provide guidance cues for endothelial cell migration during regenerative angiogenesis, and impairments in collagen lead to severe vascular defects and hindered fin regeneration.
SCIENTIFIC REPORTS
(2021)
Article
Oncology
Seung-Mo Kim, Eun-Jung Lim, Ki-Chun Yoo, Yi Zhao, Jae-Hyeok Kang, Eun-Ji Lim, Incheol Shin, Seok-Gu Kang, Han Woong Lim, Su-Jae Lee
Summary: This study investigates the molecular mechanisms of ECM remodeling and the interaction between GBM-educated MSLCs and GBMs in tumor progression. The findings provide mechanistic insights into the pro-infiltrative tumor microenvironment and highlight a potential therapeutic target for suppressing GBM infiltration.
CLINICAL AND TRANSLATIONAL MEDICINE
(2022)
Review
Biochemistry & Molecular Biology
Wei Wang, Xiangjun Wang, Feng Yao, Chao Huang
Summary: The lysyl oxidase (LOX) family plays a crucial role in tumor microenvironment remodeling and is closely associated with tumor invasion, metastasis, and immunomodulation. The family may serve as an effective target for tumor therapy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Medicine, Research & Experimental
Romane Vizier, Anais-Rachel Garnier, Alexandre Dias, Mathieu Moreau, Michael Claron, Bertrand Collin, Franck Denat, Pierre-Simon Bellaye, Victor Goncalves
Summary: In this study, an antibody-based radiotracer targeting LOXL2 was developed for SPECT/CT imaging of pulmonary fibrosis. The radiotracer showed high lung uptake in fibrotic areas and effectively detected the antifibrotic activity of nintedanib in a preclinical model of pulmonary fibrosis induced by bleomycin.
MOLECULAR PHARMACEUTICS
(2023)
Review
Biochemistry & Molecular Biology
Carolina Anazco, Janin Riedelsberger, Lorenzo Vega-Montoto, Armando Rojas
Summary: Collagen is a vital structural protein in mammals and is strengthened by complex enzymatic and non-enzymatic reactions, such as covalent cross-linking and glycation. This review article explores the amine oxidase-like activity of plant polyphenols and their potential as antiglycation agents. It also discusses the molecular mechanisms by which specific flavonoids impact or protect the normal collagen cross-linking process, with the aim of promoting the stabilization of highly organized collagen fibrils.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Multidisciplinary
Jinwon Park, Jung Suk Kim, Geon Yang, Hobin Lee, Gayong Shim, Jaiwoo Lee, Yu Kyoung Oh
Summary: The researchers designed LOX-responsive nanoparticles to interact with the collagen matrix in the tumor microenvironment. In vitro experiments showed that these nanoparticles could bind to LOX-secreting CT26 cells, but this binding was blocked by a LOX inhibitor. In mouse experiments, the addition of the LOX inhibitor did not significantly change the antitumor efficacy of the nanoparticles. However, without the LOX inhibitor, a single administration of the nanoparticles provided sustained responsiveness to near-infrared irradiation and ablation of primary tumors.
JOURNAL OF CONTROLLED RELEASE
(2023)
Article
Biochemistry & Molecular Biology
Talita de S. Laurentino, Roseli da S. Soares, Antonio M. Lerario, Suely K. N. Marie, Sueli M. Oba-Shinjo
Summary: LOXL3, a member of the lysyl oxidase family, plays a role in collagen and elastin crosslinking. In tumors, high LOXL3 expression levels are associated with genomic stability and cell invasion. Silencing of LOXL3 in glioblastoma cells leads to decreased invasion potential and potential improvement in patient prognosis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Immunology
Joel Finney, Chen-Hao Yeh, Garnett Kelsoe, Masayuki Kuraoka
IMMUNOLOGICAL REVIEWS
(2018)
Article
Biochemistry & Molecular Biology
Joel Finney, Hee-Jung Moon, Trey Ronnebaum, Mason Lantz, Minae Mure
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2014)
Article
Biochemistry & Molecular Biology
Hee-Jung Moon, Joel Finney, Trey Ronnebaum, Minae Mure
BIOORGANIC CHEMISTRY
(2014)
Article
Biochemistry & Molecular Biology
Li Xu, Eden P. Go, Joel Finney, HeeJung Moon, Mason Lantz, Kathryn Rebecchi, Heather Desaire, Minae Mure
JOURNAL OF BIOLOGICAL CHEMISTRY
(2013)
Article
Biochemistry & Molecular Biology
Hee-Jung Moon, Joel Finney, Li Xu, David Moore, Danny R. Welch, Minae Mure
JOURNAL OF BIOLOGICAL CHEMISTRY
(2013)
Review
Virology
Joel Finney, Garnett Kelsoe
Review
Immunology
Joel Finney, Akiko Watanabe, Garnett Kelsoe, Masayuki Kuraoka
IMMUNOLOGICAL REVIEWS
(2019)
Article
Immunology
Joel Finney, Guang Yang, Masayuki Kuraoka, Shengli Song, Takuya Nojima, Laurent Verkoczy, Daisuke Kitamura, Barton F. Haynes, Garnett Kelsoe
JOURNAL OF IMMUNOLOGY
(2019)
Editorial Material
Immunology
Joel Finney, Garnett Kelsoe
Article
Immunology
Joel Finney, Garnett Kelsoe
Summary: This study demonstrates that transcriptional programming can prevent B cell differentiation into plasma cells, prolonging their culture time, and provides a potential stable B cell line for various applications.
JOURNAL OF IMMUNOLOGY
(2021)
Article
Immunology
Chen-Hao Yeh, Joel Finney, Takaharu Okada, Tomohiro Kurosaki, Garnett Kelsoe
Summary: The study found that GC-resident and nonresident Tfh cells have distinct developmental requirements and activities, implying distinct functions.