Journal
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
Volume 103, Issue 2, Pages 917-927Publisher
SPRINGER
DOI: 10.1007/s00253-018-9497-0
Keywords
Rhodobacter sphaeroides; Antioxidant activities; Oxidative stress; Caco-2 cell
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Funding
- China Scholarship Council Foundation [201708110129]
- Beijing Natural Science Foundation [6173033]
- National Natural Science Foundation of China [31570494]
- Beijing Municipal Commission of Education [KM20131141 7007, PXM2013_ 014209_ 07_ 000082]
- Facing Characteristic Discipline of Beijing Union University
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The present study aimed to evaluate the in vitro antioxidant activities and the protective effect of Rhodobacter sphaeroides on H2O2-induced oxidative stress in Caco-2 cells. The results showed that the antioxidant action of R. sphaeroides varied with different cell concentrations and treatments. Also, the intact cells and intracellular cell-free extracts showed better antioxidant activities. Caco-2 cell-based oxidative stress model was developed by optimizing H2O2 concentration and culture time with the half lethal dose and methyl thiazolyl tetrazolium. By increasing the activity of endogenous antioxidant enzymes superoxide dismutase, catalase, and glutathione peroxidase, upregulating the antioxidant ability of the anti-superoxide anion and anti-hydroxyl radical, R. sphaeroides, especially the mutant strain R. sphaeroides (CGMCC No. 8513), exhibited significant protective activity against H2O2-induced oxidative stress in Caco-2 cells. Taken together, R. sphaeroides (CGMCC No. 8513) exhibits strong antioxidant activities and is a candidate to be investigated as a potential probiotic in the future.
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