Review
Cell Biology
Anita V. Kumar, Joslyn Mills, Louis R. Lapierre
Summary: Efficient proteostasis is crucial for cellular maintenance, and the protein p62 plays a key role as a selective autophagy receptor and also participates in other cellular processes. p62 has context-dependent impacts on aging and diseases, reflecting its involvement in maintaining proteostasis. This review highlights recent advances in understanding p62's functions and mechanisms and its implications on aging and neurodegeneration.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Cell Biology
Yinfeng Xu, Yaosen Wu, Lei Wang, Zhuo Ren, Lijiang Song, Hui Zhang, Chuying Qian, Qian Wang, Zhengfu He, Wei Wan
Summary: The study uncovered a regulatory role of autophagy in RNA metabolism, showing that autophagy-deficient cells have higher levels of 47S precursor rRNA due to accumulation of SQSTM1. This accumulation potentiates MTORC1 signaling, leading to increased rRNA transcription from rDNA and promoting protein synthesis and cell proliferation.
Review
Cell Biology
Xiaolan Fan, Tiantian Huang, Yingdong Tong, Ziqiang Fan, Ziyue Yang, Deying Yang, Xueping Mao, Mingyao Yang
Summary: p62, also known as SQSTM1, is a crucial predictor of autophagic flux and plays a role in maintaining protein homeostasis in cells. It has the ability to resist oxidative stress, and its multiple domains allow it to interact with vital signaling pathways, influencing cell proliferation, apoptosis, and survival. Recent studies have also shown its involvement in the aging process.
AGEING RESEARCH REVIEWS
(2022)
Article
Biochemistry & Molecular Biology
Yasukazu Takanezawa, Ryohei Harada, Yoshio Shibagaki, Yui Kashiwano, Ryosuke Nakamura, Yuka Ohshiro, Shimpei Uraguchi, Masako Kiyono
Summary: p62 interacts with NEDD4 to alleviate MeHg toxicity through the formation of a complex.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Oncology
Sheng Wang, Wei Feng, Wulin Wang, Xiaoman Ye, Hao Chen, Chunzhao Yu
Summary: This study aims to increase the sensitivity of gemcitabine chemotherapy for pancreatic cancer by silencing the Girdin gene, showing that Girdin may enhance chemotherapy resistance to gemcitabine through regulating autophagy activity.
FRONTIERS IN ONCOLOGY
(2021)
Article
Food Science & Technology
Shangyun Lu, Jiali Xu, Yang Xu, Yang Liu, Dongxing Shi, Jia Wang, Fubin Qiu
Summary: Maintaining the integrity and stability of the gut tract is crucial for overall body health. This study focused on using autophagy to balance gut homeostasis and found that glycyrol (GC), a coumarin compound from Glycyrrhiza uralensis Fisch, can activate autophagy and attenuate colon injury. By inhibiting the interaction between SQSTM1/p62 and ubiquitin-specific protease 8 (USP8), GC promotes the ubiquitination of p62 and activates autophagy. These findings suggest that GC has potential as a novel colon protector and should be further investigated.
JOURNAL OF FUNCTIONAL FOODS
(2023)
Article
Biochemistry & Molecular Biology
Toshinaru Fukae, Takeshi Miyatsuka, Miwa Himuro, Yuka Wakabayashi, Hitoshi Iida, Shuhei Aoyama, Tomoya Mita, Fuki Ikeda, Hidenori Haruna, Noriyuki Takubo, Yuya Nishida, Toshiaki Shimizu, Hirotaka Watada
Summary: Autophagy plays a crucial role in intracellular quality control, while the accumulation of p62 under autophagy-deficient conditions has little effect on 0 cell function.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Juncal Garcia-Garcia, Anne Kristin McLaren Berge, Katrine Stange Overa, Kenneth Bowitz Larsen, Zambarlal Bhujabal, Andreas Brech, Yakubu Princely Abudu, Trond Lamark, Terje Johansen, Eva Sjottem
Summary: TRIM27 is a multifunctional protein involved in various cellular functions. It has been found to facilitate mitochondrial clustering via SQSTM1/p62 and promote mitophagy by stabilizing phosphorylated TBK1 on mitochondria.
Article
Cell Biology
Hainan He, Junling Wang, Xingmei Mou, Xin Liu, Qiao Li, Mingyue Zhong, Bingbing Luo, Zhisheng Yu, Jingjing Zhang, Tian Xu, Chengli Dou, Danya Wu, Wei Qing, Linhui Wu, Kai Zhou, Zhengang Fan, Tingting Wang, Taotao Hu, Xia Zhang, Jilong Zhou, Yi-Liang Miao
Summary: This study shows that reduced autophagy in granulosa cells is negatively correlated with oocyte quality. Inhibition or genetic ablation of autophagy in granulosa cells impairs oocyte quality and fertilization ability. The adverse impact of autophagy impairment on oocyte quality is mediated by downregulated citrate levels, which can be restored by exogenous supplementation.
Article
Cell Biology
Paivi Yla-Anttila, Soham Gupta, Maria G. Masucci
Summary: Macroautophagy plays a critical role in controlling viral infections, with viruses evolving strategies to interfere with this process. The Epstein-Barr virus-encoded BPLF1 protein regulates selective autophagy, promoting infection and viral production by targeting the cellular defense mechanism.
Article
Multidisciplinary Sciences
Yi Wang, Juan Gao, Bojun Fan, Yuemin Hu, Yuefei Yang, Yajie Wu, Feng Li, Huiming Ju
Summary: This study investigated the effects of different levels of autophagy induced by serum starvation on the metabolism and differentiation of porcine skeletal muscle satellite cells (SMSCs). Autophagy was induced in SMSCs by controlling the serum concentration, leading to changes in markers of apoptosis, membrane potential, reactive oxygen species (ROS), ATP, myogenic differentiation, and lipogenic differentiation. The study found that mild autophagy promoted metabolism and differentiation in SMSCs, while severe autophagy resulted in increased apoptosis, abnormal metabolism, and suppressed differentiation. These findings suggest that autophagy plays a role in skeletal muscle development and can potentially be targeted to improve meat production traits in domestic pigs.
SCIENTIFIC REPORTS
(2023)
Article
Biochemistry & Molecular Biology
Young Rae Jo, Yuna Oh, Young Hee Kim, Yoon Kyung Shin, Hye Ran Kim, Hana Go, Jaekyoon Shin, Hye Ji Park, Hyongjong Koh, Jong Kuk Kim, Jung Eun Shin, Kyung Eun Lee, Hwan Tae Park
Summary: DSCs utilize autophagy-related genes to form secretory phagophores and tubular phagophores, enabling the exocytosis of large myelin chambers. This autophagy-mediated myelin clearance mechanism is a novel response of DSCs to nerve damage.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Lanxin Bao, Jing Ye, Nannan Liu, Yubao Shao, Wenhao Li, Xuefei Fan, Dahai Zhao, Hongzhi Wang, Xiaoyu Chen
Summary: Resveratrol has been found to improve the progression of rheumatoid arthritis (RA) by regulating autophagy. In patients with RA-associated interstitial lung disease (RA-ILD), autophagy is enhanced in the early stage but inhibited in the late stage. However, treatment with resveratrol can improve autophagy levels, reverse the inhibition, and attenuate fibrosis.
Article
Cell Biology
David S. Schussele, Patricia K. Haller, Maximilian L. Haas, Catherine Hunter, Katharina Sporbeck, Tassula Proikas-Cezanne
Summary: This paper presents a method for single cell-based analysis of autophagy using open source CellProfiler software. The method utilizes fluorescence microscopy to detect autophagy-related proteins and provides a reliable and automated analysis of the number and size of recognized puncta. The method can be easily adapted for different autophagy markers or cell types and is suitable for high-throughput studies.
Article
Multidisciplinary Sciences
Min Ji Yoon, Boyoon Choi, Eun Jin Kim, Jiyeon Ohk, Chansik Yang, Yeon-Gil Choi, Jinyoung Lee, Chanhee Kang, Hyun Kyu Song, Yoon Ki Kim, Jae-Sung Woo, Yongcheol Cho, Eui-Ju Choi, Hosung Jung, Chungho Kim
Summary: The study reveals that UXT functions as an autophagy adaptor for p62-dependent aggrephagy, delaying motor neuron degeneration in a Xenopus model. Additionally, the cooperative relationship between molecular chaperones and the aggrephagy machinery is demonstrated for efficient removal of misfolded protein aggregates.
NATURE COMMUNICATIONS
(2021)