Review
Oncology
Matthew P. Parker, Kenneth R. Peterson, Chad Slawson
Summary: O-linked beta-N-acetylglucosamine (O-GlcNAc) is a post-translational modification linking nutrient flux to gene transcription. Aberrant O-GlcNAcylation is associated with cancer development, progression, and alterations in gene expression. O-GlcNAc cycling by OGT and OGA regulates various aspects of gene expression, including RNA polymerase function and transcription factor activity.
Article
Cell Biology
Lorela Ciraku, Emily M. Esquea, Mauricio J. Reginato
Summary: O-GlcNAcylation is a post-translational modification affecting protein stability, interactions, and phosphorylation, playing a crucial role in nutrient sensing and regulation of cancer signaling pathways. Its elevated levels have been linked to various cancers, making it a potential target for cancer treatment.
CELLULAR SIGNALLING
(2022)
Article
Biochemistry & Molecular Biology
Xiaoman He, Yongzhou Li, Qing Chen, Lei Zheng, Jianyao Lou, Chuanshuai Lin, Jiali Gong, Yi Zhu, Yulian Wu
Summary: SIRT7 expression is upregulated in pancreatic cancer cells and predicts poor prognosis. O-GlcNAcylation is an important post-translational modification of SIRT7, stabilizing SIRT7 protein by inhibiting its interaction with REG gamma. O-GlcNAcylation leads to hypoacetylation of H3K18, promoting transcriptional repression of several tumor suppressor genes.
CELL DEATH AND DIFFERENTIATION
(2022)
Article
Multidisciplinary Sciences
Li Zhu, Xiting Wei, Jianming Cong, Jing Zou, Lihao Wan, Shutong Xu
Summary: The authors present the crystal structure of the Arabidopsis SPY/GDP complex, revealing the substrate recognition and enzyme mechanism of SPY. This study provides insights into the glycan donor substrate selection in GT41 proteins.
NATURE COMMUNICATIONS
(2022)
Article
Chemistry, Multidisciplinary
Jiahui He, Zhiya Fan, Yinping Tian, Weiwei Yang, Yichao Zhou, Qiang Zhu, Wanjun Zhang, Weijie Qin, Wen Yi
Summary: Light control of OGT activity in cells has been achieved by replacing a catalytically essential lysine residue with a genetically encoded photocaged lysine, providing a valuable chemical tool to control cellular O-GlcNAc with spatiotemporal precision and aiding in a better understanding of O-GlcNAc function.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Review
Biochemical Research Methods
Graham A. Heieis, Bart Everts
Summary: O-GlcNAcylation plays a crucial role in malignant transformation in tumors and its function in the immune system remains largely unknown. Recent discoveries suggest that O-GlcNAcylation may negatively affect overall antitumor immune responses in both tumors and immune cells. Interference with O-GlcNAcylation holds promise for cancer treatment from both perspectives.
CURRENT OPINION IN BIOTECHNOLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
Laila T. Kirkpatrick, Morgan R. Daughtry, Samer El-Kadi, Tim Hao Shi, David E. Gerrard
Summary: This study investigates the role of O-GlcNAcylation in porcine myogenesis. The findings show that elevated O-GlcNAcylation impairs muscle cell differentiation by downregulating the expression of myogenin and inhibiting Akt phosphorylation. In contrast, inhibition of O-GlcNAc transferase has little impact on myoblast proliferation or differentiation. Additionally, the study demonstrates that O-GlcNAcylation plays a critical role in muscle regeneration in vivo. These findings suggest that O-GlcNAcylation serves as a nutrient sensor and regulates muscle growth in agriculturally important animals.
JOURNAL OF ANIMAL SCIENCE
(2022)
Article
Chemistry, Analytical
Senhan Xu, Kejun Yin, Ronghu Wu
Summary: Protein O-GlcNAcylation plays crucial roles in mammalian cells by regulating signal transduction and gene expression. In this study, a method was developed to globally and site-specifically characterize protein co-translational OGlcNAcylation, which is challenging due to the low abundance of O-GlcNAcylated proteins. By integrating selective enrichment, a boosting approach, and multiplexed proteomics, more than 180 co-translational O-GlcNAcylated proteins were identified. This method enhances our understanding of this important protein modification.
ANALYTICAL CHEMISTRY
(2023)
Article
Oncology
Ye Chen, Jiaxin Bei, Mingyu Liu, Jingjun Huang, Lulu Xie, Wensou Huang, Mingyue Cai, Yongjian Guo, Liteng Lin, Kangshun Zhu
Summary: This study reveals that sublethal heat stress enhances the Warburg effect of HCC cells through O-GlcNAcylation, contributing to thermotolerance and invasion. Inhibition of O-GlcNAcylation weakens the Warburg effect, impairs thermotolerance and EMT in HCC cells, ultimately preventing tumor recurrence and metastasis. Sublethal heat stress-induced O-GlcNAcylation regulates the Warburg effect by promoting HIF-1α stability.
Article
Biochemistry & Molecular Biology
Wei Tan, Pei Jiang, Wanjun Zhang, Zhaohua Hu, Shaofeng Lin, Lulu Chen, Yingge Li, Changmin Peng, Zhuqing Li, Aihua Sun, Yali Chen, Wenge Zhu, Yu Xue, Yi Yao, Xiangpan Li, Qibin Song, Fuchu He, Weijie Qin, Huadong Pei
Summary: This study demonstrates that the posttranslational modification of SRPK2 by O-GlcNAc plays a key role in regulating de novo lipogenesis by controlling pre-mRNA splicing. O-GlcNAcylation of SRPK2 at a nuclear localization signal and subsequent nuclear import through binding to importin a reveals a general mechanism for protein nuclear import in cells. This work highlights the important role of O-GlcNAc in posttranscriptional regulation and suggests importin a as a reader of an O-GlcNAcylated nuclear localization signal.
Review
Biochemistry & Molecular Biology
Rodrigo P. Silva-Aguiar, Diogo B. Peruchetti, Ana Acacia S. Pinheiro, Celso Caruso-Neves, Wagner B. Dias
Summary: Kidneys maintain internal stability by regulating body fluid composition through the correlation between structure and function in the nephron. Kidney diseases have a global impact on healthcare programs, and there are limited treatment options available. Protein O-GlcNAcylation, a post-translational modification involved in regulating various cell functions, is associated with the development of chronic degenerative diseases and has implications in cardiovascular, neurodegenerative, and metabolic diseases. This review discusses the impact of protein O-GlcNAcylation on renal function, disease conditions, and future directions in the field.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Medicine, Research & Experimental
Xu Xu, Xiaona Wang, Qihui Chen, Aman Zheng, Donglu Li, Ziqi Meng, Xinran Li, Hanchen Cai, Wangzhi Li, Shiyuan Huang, Fan Wang
Summary: This study finds that Sp1 is upregulated in cervical cancer and promotes the development of cervical tumors by regulating the mitochondrial network and reprogramming glucose metabolism. Inhibition of Sp1 expression effectively suppresses cell proliferation, suggesting that targeting Sp1 could be an effective strategy for the treatment of cervical cancer.
JOURNAL OF TRANSLATIONAL MEDICINE
(2023)
Article
Cell Biology
Fan Yu, Qianping Zhang, Hanyu Liu, Jinming Liu, Song Yang, Xiaofan Luo, Wei Liu, Hao Zheng, Qiqi Liu, Yunxi Cui, Guo Chen, Yanjun Li, Xinglu Huang, Xiyun Yan, Jun Zhou, Quan Chen
Summary: The O-GlcNAcylation of proteins plays a role in regulating ferroptosis by modulating ferritinophagy and mitophagy. Inhibition of O-GlcNAcylation promotes ferritinophagy and enhances mitophagy, resulting in the accumulation of labile iron and increased sensitivity to ferroptosis.
Article
Pharmacology & Pharmacy
Feinan Zhou, Jia Ma, Yongfu Zhu, Tianming Wang, Yue Yang, Yehan Sun, Youmou Chen, Hang Song, Xingxing Huo, Jianye Zhang
Summary: Glycosylation is a critical post-translational modification that affects protein function and regulates biological processes. Protein O-GlcNAc glycosylation modifications play an important role in signal transduction within cells, competing with phosphorylation modifications to immediately regulate protein activity and participate in cellular metabolic and signal transduction pathways. These modifications have a pivotal role in the regulation of tumors, diabetes, and other diseases.
PHARMACOLOGICAL RESEARCH
(2022)
Article
Multidisciplinary Sciences
Hongshuo Zhang, Zhen Li, Yufei Wang, Ying Kong
Summary: O-GlcNAcylation is a crucial protein modification that is associated with various diseases, regulating cellular metabolism and maintaining homeostasis by integrating signaling pathways. It plays a key role in cellular nutrient sensing and metabolic regulation.
Article
Multidisciplinary Sciences
Chang Hoon Ha, Seok Cheol Lee, Sunghyen Kim, Jihwa Chung, Hasuk Bae, Kihwan Kwon
SCIENTIFIC REPORTS
(2015)
Article
Biochemistry & Molecular Biology
Bohyun Yoon, Inki Kim, Ja-Ae Nam, Hyo-Ihl Chang, Chang Hoon Ha
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2016)
Article
Oncology
Je-won Ryu, Sung Sik Choe, Seung-Hee Ryu, Eun-Young Park, Byoung Wook Lee, Tae Keun Kim, Chang Hoon Ha, Sang-wook Lee
Article
Multidisciplinary Sciences
Seung-Hyun Lee, Jaesung Seo, Soo-Yeon Park, Mi-Hyeon Jeong, Hyo-Kyoung Choi, Chan Joo Lee, Mi Jeong Kim, Garam Guk, SooYeon Lee, Hyewon Park, Jae-Wook Jeong, Chang Hoon Ha, Sungha Park, Ho-Geun Yoon
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2018)
Article
Hematology
Chang Hoon Ha, Bong Sook Jhun, Hung-Ying Kao, Zheng-Gen Jin
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
(2008)
Article
Hematology
Weiye Wang, Chang Hoon Ha, Bong Sook Jhun, Chelsea Wong, Mukesh K. Jain, Zheng-Gen Jin
Article
Biochemistry & Molecular Biology
Chang Hoon Ha, Anton M. Bennett, Zheng-Gen Jin
JOURNAL OF BIOLOGICAL CHEMISTRY
(2008)
Article
Biochemistry & Molecular Biology
Chang Hoon Ha, Weiye Wang, Bong Sook Jhun, Chelsea Wong, Angelika Hausser, Klaus Pfizenmaier, Timothy A. McKinsey, Eric N. Olson, Zheng-Gen Jin
JOURNAL OF BIOLOGICAL CHEMISTRY
(2008)
Article
Multidisciplinary Sciences
Chang Hoon Ha, Ji Young Kim, Jinjing Zhao, Weiye Wang, Bong Sook Jhun, Chelsea Wong, Zheng Gen Jin
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2010)
Article
Multidisciplinary Sciences
Jong Geol Lee, Chang Hoon Ha, Bohyun Yoon, Seung-A Cheong, Globinna Kim, Doo Jae Lee, Dong-Cheol Woo, Young-Hak Kim, Sang-Yoon Nam, Sang-wook Lee, Young Hoon Sung, In-Jeoung Baek
SCIENTIFIC REPORTS
(2019)
Article
Biochemistry & Molecular Biology
Bo Hyun Yoon, Seung Min Lee, Hyo-Ihl Chang, Chang Hoon Ha
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2019)
Article
Biochemistry & Molecular Biology
Eun Sil Kim, Bo Hyun Yoon, Seung Min Lee, Min Choi, Eun Hye Kim, Byong-Wook Lee, Sang-Yeob Kim, Chan-Gi Pack, Young Hoon Sung, In-Jeoung Baek, Chang Hee Jung, Tae-Bum Kim, Jin-Yong Jeong, Chang Hoon Ha
Summary: This study reveals the association between CTRP9 gene deficiency and gut microbiota distribution in patients with atherosclerosis, confirms the influence of gut microbiota on the progression of atherosclerosis, and suggests restoring gut microbial homeostasis as a potential therapeutic strategy.
EXPERIMENTAL AND MOLECULAR MEDICINE
(2022)
Article
Multidisciplinary Sciences
Seung Min Lee, Jin Woo Lee, Inki Kim, Dong-Cheol Woo, Chan-Gi Pack, Young Hoon Sung, In-Jeoung Baek, Chang Hee Jung, Young-Hak Kim, Chang Hoon Ha
Summary: CTRP9 induces a vasoprotective response via the AMPK/HDAC7/p38 MAPK pathway in vascular endothelial cells, promoting angiogenesis and preventing atherosclerosis and myocardial infarction (MI).
Article
Biochemistry & Molecular Biology
Soyoung Kim, Jaeseok Han, Young-Ho Ahn, Chang Hoon Ha, Jung Jin Hwang, Sang-Eun Lee, Jae-Joong Kim, Nayoung Kim
Summary: Hypoxia induces cellular stress and can lead to various pathological consequences. This study identifies miR-34c as a hypoxia-induced microRNA that regulates cell survival by inhibiting BCL2 expression and activating autophagy. Overexpression of miR-34c inhibits apoptosis and increases the number of viable cells in blood vessels, suggesting it as a potential therapeutic target for hypoxic and ischemic diseases.
MOLECULES AND CELLS
(2022)
Article
Biochemistry & Molecular Biology
Soojung Hahn, Gyuri Kim, Sang-Man Jin, Jae Hyeon Kim
Summary: This study utilized three-dimensional intestinal organoids to investigate the effects of metformin on inflammatory bowel disease (IBD) and found that metformin can enhance intestinal barrier function and reduce levels of inflammatory cytokines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
V. V. Sudarev, M. S. Gette, S. V. Bazhenov, O. M. Tilinova, E. V. Zinovev, I. V. Manukhov, A. I. Kuklin, Yu. L. Ryzhykau, A. V. Vlasov
Summary: This study investigated the self-assembly processes of ferritin-based protein complexes and obtained structurally characterized oligomeric states. These results provide new potential and opportunities for the application of ferritin in various fields.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Yalda Sabaghi, Farnaz Pourfarzad, Leila Zolghadr, Azita Bahrami, Tahereh Shojazadeh, Alireza Farasat, Nematollah Gheibi
Summary: p-Coumaric acid (p-CA) is a plant compound with anti-cancer activities. This study designed a nano-liposomal carrier containing p-CA to enhance its effectiveness against melanoma cells. The findings showed that the liposomal form of p-CA had a greater impact on the cells. Kinetic modeling indicated that the best fitting model was zero-order.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
M. D. Nazmul Hasan, Md Mahfuzur Rahman, Al Asmaul Husna, Nobuhiro Nozaki, Osamu Yamato, Naoki Miura
Summary: This study investigated the expression of ncRNAs other than miRNAs in different histologic subtypes of canine mammary gland tumors (MGT). Three aberrantly expressed ncRNAs were identified as potential biomarkers for differentiating MGT subtypes. YRNA and tRFs expression levels were found to be decreased in metastatic compared to primary MGT cell lines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Seine A. Shintani
Summary: In this study, the researchers used signal analysis to study the instantaneous amplitude and phase of sarcomeric oscillations in skeletal muscle. They identified two types of oscillations, sarcomeric oscillations and sarcosynced oscillations, and visualized their behavior during propagating waves. The researchers discovered the presence of sarcomeric defect holes and sarcomeric collision holes, which are important indicators for understanding the oscillation properties of sarcomeres. This finding has important implications for improving our understanding of muscle function and its regulatory mechanisms.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Shuanglin Zhang, Yuzhong Jia, Guolan Ma, Yanyan Yang, Zhenzhen Cao, Antao Luo, Zefu Zhang, Shihan Li, Jie Wen, Hanfeng Liu, Jihua Ma
Summary: Bupleurum is an antiarrhythmic agent that may exert its effects by inhibiting L-type calcium channels.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Tomotaka Ohkubo, Yasuhiko Matsumoto, Hiroaki Sasaki, Kaoru Kinoshita, Yuki Ogasawara, Takashi Sugita
Summary: This study found that Citrobacter koseri inhibits the growth of Staphylococcus epidermidis, disrupting the balance between S. epidermidis and Staphylococcus aureus, and exacerbating inflammation in atopic dermatitis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Toshifumi Asano, Philipp Sasse, Takao Nakata
Summary: A Cre recombination-based fluorescent reporter system was developed to monitor cell-cell fusion. The system successfully detected the formation of multinuclear myotubes and placental syncytiotrophoblast. This tool could facilitate the study of cell-to-cell fusion.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Ke Shi, Yunlong Shan, Xiao Sun, Kuida Chen, Qiong Luo, Qiang Xu
Summary: This study found that low expression of TP53INP2 is associated with poor survival in colorectal cancer (CRC) patients. As the malignancy of CRC progresses, TP53INP2 expression gradually decreases. Knockdown of TP53INP2 promotes CRC cell proliferation and tumor growth. Mechanistically, TP53INP2 deficiency decreases phosphorylation of beta-catenin, leading to increased accumulation and enhanced nuclear translocation and transcriptional activity. Additionally, TP53INP2 sequesters TIM50, inhibiting its activation of beta-catenin. In conclusion, downregulation of TP53INP2 promotes CRC progression by activating beta-catenin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Martina Rossi, Fabio Tomaselli, Alejandro Hochkoeppler
Summary: Oligomeric enzymes are known for their higher catalytic rates compared to monomeric enzymes, but the extent of additivity in their activity is still not well understood. This study used tetrameric rabbit lactate dehydrogenase as a model to examine the kinetics of its catalytic action. Surprisingly, when the concentration of the limiting reactant exceeded that of a single subunit, there was a significant slowdown in the enzyme's conformational rearrangements.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Amin Sahraei, Mohammad Javad Shamsoddini, Fakhrossadat Mohammadi, Leila Hassani
Summary: This study explored the inhibitory effects of gallium curcumin, indium curcumin, and vanadyl curcumin on the amyloid fibrillation of hen egg white lysozyme, as well as the binding interactions of these metal complexes with the enzyme. The results showed that indium curcumin and vanadyl curcumin exhibited higher binding affinities and stronger inhibitory effects on amyloid fibrillation compared to gallium curcumin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Takahiro Sasaki, Yoshiki Kuse, Shinsuke Nakamura, Masamitsu Shimazawa
Summary: PGRN deficiency plays a significant role in cardiac remodeling and arrhythmias post-myocardial infarction (MI), potentially by promoting metabolic abnormalities in macrophages.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Hongwei Zhao, Yiqiang Li, Yibo Zhang, Chi Zhang
Summary: Electrical brain stimulation technology is commonly used to treat brain neurological disorders, but it can cause side effects. This study investigated the impact of electric fields on nerve fibers and revealed the possible origin of side effects. The findings provide guidance for selecting electrical parameters in clinical stimulation therapy.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Julia S. Scott, Lake-Ee Quek, Andrew J. Hoy, Johannes V. Swinnen, Zeyad D. Nassar, Lisa M. Butler
Summary: The fatty acid elongation enzyme ELOVL5 plays a critical role in promoting metastasis in prostate cancer. Knocking down ELOVL5 leads to the accumulation of malonyl-CoA, which inhibits fatty acid oxidation in mitochondria. This study highlights the importance of fatty acid elongation in regulating cell viability and provides a potential target for prostate cancer treatment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Zan Zhou, Wen-jun Jiang, Li Li, Jun-qiang Si
Summary: This study investigates the effect of noise exposure on cognitive function in mice and explores the underlying molecular mechanisms. The findings suggest that noise exposure leads to increased inflammation, increased phosphorylation of Tau protein, and decreased levels of postsynaptic density protein, resulting in cognitive impairment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)