Article
Toxicology
Ezgi Eyluel Bankoglu, Carolin Schuele, Helga Stopper
Summary: The comet assay is widely used in basic research, genotoxicity testing, and human biomonitoring. The study found that DNA damage exceeding 20-30% tail DNA caused more than 50% of cells to die, with etoposide causing slightly more cell death than H2O2 or MMS. Cells showed some repair capacity for DNA damage within a few hours after substance removal.
ARCHIVES OF TOXICOLOGY
(2021)
Article
Toxicology
Ezgi Eyluel Bankoglu, Franzisca Stipp, Johanna Gerber, Florian Seyfried, August Heidland, Udo Bahner, Helga Stopper
Summary: The comet assay is a widely used method for assessing DNA damage and repair activity in various samples, with cryopreserved blood preparations found to be suitable for such analysis. The study also revealed that ex vivo hydrogen peroxide challenge had optimal effects in PBMCs, while repair comet assays worked well in fresh PBMCs.
ARCHIVES OF TOXICOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Mahsa Karbaschi, Yunhee Ji, Mubarak A. Mujawar, Mario Mendoza, Juan S. Marquez, Apurva Sonawane, Pratikkumar Shah, Chris Ross, Shekhar Bhansali, Marcus S. Cooke
Summary: This article describes the development of an automated device for high throughput sample processing in a comet assay, which reliably assesses DNA damage in individual eukaryotic cells. The device performs as well as manual methods but with the advantages of decreased human involvement and assay run time, making it a valuable approach for high throughput DNA damage assessment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Plant Sciences
Beata P. Plitta-Michalak, Alice A. Ramos, Piotr Pupel, Marcin Michalak
Summary: The high-throughput comet assay is a sensitive tool for monitoring DNA integrity changes and assessing the viability status of plant germplasm.
Article
Genetics & Heredity
Jing Ge, Le P. Ngo, Simran Kaushal, Ian J. Tay, Elina Thadhani, Jennifer E. Kay, Patrizia Mazzucato, Danielle N. Chow, Jessica L. Fessler, David M. Weingeist, Robert W. Sobol, Leona D. Samson, Scott R. Floyd, Bevin P. Engelward
Summary: DNA damage is directly linked to aging, cancer, and other diseases, and cells have evolved highly conserved DNA repair pathways to counteract its deleterious effects. The CometChip platform offers increased throughput and sensitivity for parallel analysis of multiple DNA repair activities, expanding the utility of the comet assay for precise, high-throughput assessment of repair deficiencies in different pathways.
Article
Toxicology
Congying Zheng, Sergey Shaposhnikov, Andrew Collins, Gunnar Brunborg, Florin Oancea, Frederik-Jan Van Schooten, Roger Godschalk
Summary: DNA repair is crucial for maintaining genomic stability, and can be evaluated through comet assay-based approaches such as the cellular repair assay and the in vitro repair assay. However, a direct comparison of both assays in multiple cell lines showed no significant relationship between them. The in vitro repair assay correlated with the background level of DNA damage in the test cells and indicated that base excision repair is inducible. These findings suggest that these two assays measure different endpoints of DNA repair and should be considered complementary.
ARCHIVES OF TOXICOLOGY
(2023)
Article
Multidisciplinary Sciences
Mirko Marino, Letizia Gigliotti, Peter Moller, Patrizia Riso, Marisa Porrini, Cristian Del Bo
Summary: The comet assay is used to assess DNA damage in tissues and biological samples. This study found that storage time had varying effects on DNA damage levels in PBMCs and WB samples, with significant increases observed in WB samples stored for over 6 months. WB samples showed higher levels of DNA damage compared to PBMCs.
SCIENTIFIC REPORTS
(2021)
Review
Environmental Sciences
Aryelle Canedo, Thiago Lopes Rocha
Summary: Zebrafish have become a popular model for assessing the genotoxicity of pollutants due to their orthologous genes and involvement in repair pathways. Comet assay is the main technique used in these studies, but standardization is needed for better interpretation and comparisons. Understanding repair mechanisms in zebrafish subjected to DNA damage is essential for further research on genotoxic effects caused by pollutants.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Environmental Sciences
Marcelino Benvindo-Souza, Daiany Folador Sotero, Cirley Gomes Araujo dos Santos, Rhayane Alves de Assis, Rinneu Elias Borges, Lia Raquel de Souza Santos, Daniela de Melo e Silva
Summary: Pollution from mining activities can cause genotoxic damage to bats, particularly in species like Carollia perspicillata and Glossophaga soricina. High levels of elements such as Mn, Cr, Pb, and Zn were found in water samples from the mining area. The study suggests that bats in mining areas are susceptible to increased DNA damage, similar to what has been observed in other species.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Environmental Sciences
Felipe de Araujo Nascimento, Daniela de Melo e Silva, Thays Millena Alves Pedroso, Jheneffer Sonara Aguiar Ramos, Michelle Rocha Parise
Summary: Occupational exposure to pesticides in farmers results in significant DNA damage, showing a notable impact and variability in the studies. Smokers who are occupationally exposed to pesticides have slightly reduced DNA damage compared to non-smokers, indicating some genetic toxicity. The study demonstrates a higher risk of occupationally exposed rural workers developing related diseases due to the genotoxic effect of pesticides.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2022)
Article
Environmental Sciences
Vehbi Atahan Togay, Gulcin Yavuz Turel, Dilek Asci celik, Meltem ozgocmen, Eda Evgen Tuluceoglu, Ismail Sen, Yusuf Ayvaz
Summary: This study found that cyprodinil and thiacloprid exposure can lead to increased DNA damage risk in zebrafish, as demonstrated by comet assay. These pesticides were identified as genotoxic agents that require further investigation.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2021)
Article
Environmental Sciences
Rafael Valencia-Quintana, Mirta Milic, Stefano Bonassi, Maria Antonieta Ochoa-Ocana, Victoria Campos-Pena, Maria Guadalupe Tenorio-Arvide, Guillermo Alejandro Perez-Flores, Juana Sanchez-Alarcon
Summary: This study evaluated the effect of pesticides on genetic damage to agricultural workers in Los Reyes, Michoacan, Mexico. The results showed that pesticide use poses a risk of genetic damage to agricultural workers, but measures can be taken to reduce this risk.
Article
Pharmacology & Pharmacy
Jorid B. Sorlia, Alexander C. O. Jensen, Alicja Mortensen, Jozef Szarek, Claudia A. T. Gutierrez, Lucas Givelet, Katrin Loeschner, Charis Loizides, Iosif Hafez, George Biskos, Ulla Vogel, Niels Hadrup
Summary: By conducting nose-only inhalation tests on mice, we found that the air concentration of molybdenum disulphide particles has no observed adverse effect on body weight gain and genotoxicity below 13mg/m³, while the effects on respiratory function suggest this level as the lowest observed adverse effect concentration.
Article
Environmental Sciences
Marcelino Benvindo-Souza, Akemi Vieira Hosokawa, Cirley Gomes Araujo dos Santos, Rhayane Alves de Assis, Thays Millena Alves Pedroso, Rinneu Elias Borges, Susi Missel Pacheco, Lia Raquel de Souza Santos, Daniela de Melo e Silva
Summary: Habitat loss and fragmentation pose significant threats to biodiversity. The use of bioindicators can provide insight into the health of the environment. A study conducted in agricultural areas found higher genotoxic damage in bats compared to a control area.
ENVIRONMENTAL POLLUTION
(2022)
Article
Biochemistry & Molecular Biology
Lise Barbe, Stephanie Lam, Austin Holub, Zohreh Faghihmonzavi, Minnie Deng, Rajshri Iyer, Steven Finkbeiner
Summary: DNA damage is a common cellular feature in cancer and neurodegenerative disease, but there is a lack of fast and accurate methods for quantifying DNA damage. The comet assay is a biochemical tool that measures DNA damage based on the migration of broken DNA strands towards a positive electrode, creating a quantifiable 'tail' behind the cell. However, the analysis of comets using existing open-source algorithms is time-consuming and requires manual curation. To overcome these limitations, we developed AutoComet, an open-source algorithm that automates comet segmentation and quantification of tail parameters. AutoComet significantly improves segmentation accuracy and reduces analysis time over ten-fold, providing a fast and unbiased method for quantifying DNA damage.
Article
Biophysics
Xinran Lu, David K. Wood, John M. Higgins
BIOPHYSICAL JOURNAL
(2016)
Review
Oncology
Eva A. Turley, David K. Wood, James B. McCarthy
Review
Hematology
Xinran Lu, Michelle M. Galarneau, John M. Higgins, David K. Wood
Article
Cell Biology
Marie-Elena Brett, Heather E. Bomberger, Geneva R. Doak, Matthew A. Price, James B. McCarthy, David K. Wood
INTEGRATIVE BIOLOGY
(2018)
Article
Hematology
Xinran Lu, Anwesha Chaudhury, John M. Higgins, David K. Wood
AMERICAN JOURNAL OF HEMATOLOGY
(2018)
Article
Cell Biology
Alexandra L. Crampton, Katherine A. Cummins, David K. Wood
INTEGRATIVE BIOLOGY
(2018)
Article
Oncology
Somsak Prasongtanakij, Usanarat Anurathapan, Thitinee Vanichapol, Bunyada Jittorntrum, Korakot Atjanasuppat, Pongpak Pongpitcha, Samart Pakakasama, Duantida Songdej, Nongnuch Sirachainan, Karan Paisooksantivatana, Suparerk Borwaornpinyo, Borje S. Andersson, Suradej Hongeng
Summary: This study aimed to design and manufacture CD19 CAR-modified T cells for clinical use in Thailand and investigate the impact of different spacer lengths on cytotoxic potential. The results showed that all CD19 CAR T cell constructs exhibited similar levels of cytotoxicity, and the intermediate length spacer construct was selected for clinical application due to its higher transduction efficiency and slower initial proliferation.
ASIA-PACIFIC JOURNAL OF CLINICAL ONCOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Supasek Kongsomros, Ampa Suksatu, Phongthon Kanjanasirirat, Suwimon Manopwisedjaroen, Somsak Prasongtanakij, Kedchin Jearawuttanakul, Suparerk Borwornpinyo, Suradej Hongeng, Arunee Thitithanyanont, Somchai Chutipongtanate
Summary: The study discovered that calpeptin may be a promising candidate for anti-SARS-CoV-2 drug, showing potent inhibition on viral activity and production, and enhanced effects when used in combination with remdesivir.
Article
Public, Environmental & Occupational Health
Supranee Thongpradit, Somsak Prasongtanakij, Supanart Srisala, Yothin Kumsang, Suwannee Chanprasertyothin, Pairoj Boonkongchuen, Dhanesh Pitidhammabhorn, Parnrudee Manomaipiboon, Peeraya Somchaiyanon, Siriwan Chandanachulaka, Taiyatach Hirunrueng, Boonsong Ongphiphadhanakul
Summary: The study investigates the detection of SARS-CoV-2 in wastewater during the early stages of the second outbreak wave in Thailand. The results show that wastewater surveillance is sensitive and can detect the virus even in places with low prevalence of COVID-19 and high ambient temperature.
JOURNAL OF ENVIRONMENTAL AND PUBLIC HEALTH
(2022)
Article
Surgery
Jackrapong Bruminhent, Chavachol Setthaudom, Pattaraphorn Phornkittikorn, Pongsathon Chaumdee, Somsak Prasongtanakij, Supanart Srisala, Kumthorn Malathum, Sarinya Boongird, Arkom Nongnuch, Montira Assanatham, Laor Nakgul, Nutaporn Sanmeema, Angsana Phuphuakrat, Sasisopin Kiertiburanakul
Summary: In kidney transplant recipients, the immunogenicity following an additional dose of COVID-19 vaccine was investigated. The study found that there were no significant differences in immune responses between recipients of mRNA vaccines and viral vector vaccines, indicating both types of vaccines were effective in eliciting immunity in this population.
AMERICAN JOURNAL OF TRANSPLANTATION
(2022)
Article
Cell Biology
Dean Thumkeo, Siwakorn Punyawatthananukool, Somsak Prasongtanakij, Ryuma Matsuura, Kentaro Arima, Huan Nie, Rie Yamamoto, Naohiro Aoyama, Hisao Hamaguchi, Shingo Sugahara, Shinobu Takeda, Varodom Charoensawan, Atsushi Tanaka, Shimon Sakaguchi, Shuh Narumiya
Summary: In the tumor microenvironment, active inflammation and immunosuppression occur simultaneously and are regulated by the PGE(2)-EP2/EP4 signaling pathway. This study demonstrates that EP2/EP4 expression is strongly correlated with gene signatures of active inflammation and the mregDC-Treg axis, and has significant prognostic value in various human cancers.
Article
Environmental Sciences
Supranee Thongpradit, Somsak Prasongtanakij, Supanart Srisala, Suwannee Chanprasertyothin, Ekawat Pasomsub, Boonsong Ongphiphadhanakul
Summary: The SARS-CoV-2 virus can be detected in wastewater, and the use of an automated CLEIA method can reduce the turnaround time and improve the monitoring performance.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2022)
Article
Immunology
Sarinya Boongird, Chavachol Setthaudom, Rungthiwa Kitpermkiat, Somsak Prasongtanakij, Supanart Srisala, Piyatida Chuengsaman, Arkom Nongnuch, Montira Assanatham, Sasisopin Kiertiburanakul, Kumthorn Malathum, Angsana Phuphuakrat, Jackrapong Bruminhent
Summary: This study assessed the changes in humoral and cell-mediated immunity in dialysis patients 6 months after receiving the COVID-19 vaccine. The results showed a significant decline in humoral immunity, while cell-mediated immunity remained stable. The study suggests considering a booster dose for dialysis patients 3 months after the initial vaccination.
Review
Oncology
Geneva R. Doak, Kathryn L. Schwertfeger, David K. Wood
Article
Hematology
Marcus A. Carden, Meredith E. Fay, Xinran Lu, Robert G. Mannino, Yumiko Sakurai, Jordan C. Ciciliano, Caroline E. Hansen, Satheesh Chonat, Clinton H. Joiner, David K. Wood, Wilbur A. Lam