Review
Virology
Makram Mghezzi-Habellah, Lea Prochasson, Pierre Jalinot, Vincent Mocquet
Summary: This review discusses how viruses interfere with the karyopherin CRM1 to promote viral replication, the impact of this interference on the relocalization of cellular factors and viral replication, and the potential benefits of CRM1 inhibitors as antiviral treatments.
Review
Biochemistry & Molecular Biology
Arvind Arul Nambi Rajan, Ben Montpetit
Summary: The DEAD-box protein Dbp5 is a member of the SFII helicase superfamily and plays multiple important roles in gene expression, including regulating mRNA export, non-coding RNA export, translation, transcription, and DNA metabolism. It has the ability to function in different cellular compartments and modulate various stages of RNA lifecycle. Despite over 20 years of research on Dbp5, unanswered questions remain regarding its localization, substrate selection, and mechanistic principles in gene expression.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
Article
Multidisciplinary Sciences
James M. Burke, Alison R. Gilchrist, Sara L. Sawyer, Roy Parker
Summary: The study demonstrates that RNase L-mediated mRNA decay does not completely limit protein synthesis of dengue virus and influenza A virus, as their mRNAs largely escape this process. However, activation of RNase L inhibits nuclear mRNA export, thereby restricting influenza A virus protein synthesis and cytokine production.
Article
Virology
Clifton L. Ricana, Marc C. Johnson
Summary: This study describes a nuclear cycling-deficient RSV Gag mutant with similar plasma membrane binding and genome incorporation to wild-type virus, suggesting that RSV Gag nuclear cycling is not strictly required for RSV replication. This mutant provides insights into viral trafficking evolution and may present a model intermediate to cis- and trans-acting mechanisms for gRNA export.
JOURNAL OF VIROLOGY
(2021)
Article
Cell Biology
Xiaohu Lin, Qianqian Pang, Jie Hu, Jiaqi Sun, Siya Dai, Yijia Yu, Jinghong Xu
Summary: Sentrin/small ubiquitin-like modifier (SUMO) is a critical mediator in human diseases, including cancer and fibrosis. However, the mechanism of sumoylation in keloids is not well understood. We found that SUMO and RanGAP1 synergistically regulate keloids by modulating the nuclear export of Smad4. This study provides new insights into the mechanisms of keloids and nucleocytoplasmic transport.
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
(2023)
Review
Parasitology
Susanne Kramer
Summary: The passage of mRNAs through nuclear pores into the cytoplasm is essential for all eukaryotes, with mRNA export tightly connected to nuclear mRNA processing. Trypanosoma brucei exhibits unusual features in nuclear mRNA processing, lacking key complexes and proteins found in opisthokonts. Further investigation is needed to determine the extent of mRNA export regulation in trypanosomes and the evolutionary implications of mRNA export across eukaryotes.
Review
Cell Biology
Pierre Bensidoun, Daniel Zenklusen, Marlene Oeffinger
Summary: The nuclear pore complex (NPC) is the central gate for mRNA transportation from the nucleus to the cytoplasm. The export competence of mRNAs is linked to upstream nuclear processes, and recent evidence suggests that the NPC may be more heterogeneous than previously believed, providing functional plasticity to mRNA export. Interconnected processes of nuclear mRNA metabolism and NPC heterogeneity play crucial roles in regulating export competence.
WILEY INTERDISCIPLINARY REVIEWS-RNA
(2021)
Article
Biochemistry & Molecular Biology
Shawn Gray, Wenxiang Cao, Ben Montpetit, Enrique M. De La Cruz
Summary: A kinetic and equilibrium analysis of the Gle1-activated Dbp5 ATPase cycle in Saccharomyces cerevisiae reveals that Gle1 activates Dbp5 by modulating ATP binding and P-i release. These findings provide insights into the roles of Gle1 in regulating Dbp5-mediated RNA export and other gene expression functions.
NUCLEIC ACIDS RESEARCH
(2022)
Review
Oncology
Katherine L. B. Borden
Summary: This review highlights the importance of RNA export in cellular trafficking and the aberrant phenomena observed in cancer. Studies on RNA export machinery and relevant NPC components offer potential therapeutic strategies for targeting cancer.
Article
Virology
Margarita Valdes Aleman, Luca D. Bertzbach, Thomas Speiseder, Wing Hang Ip, Ramon A. Gonzalez, Thomas Dobner
Summary: During human adenovirus infection, the late phase of viral replication results in a significant increase in viral late mRNA abundance, which outcompetes cellular mRNA biogenesis rather than selectively exporting viral mRNA.
Review
Virology
Satarupa Sarkar, Kannan Balakrishnan, Kumaraswami Chintala, Krishnaveni Mohareer, Tom Luedde, Ananda Ayyappan Jaguva Vasudevan, Carsten Munk, Sharmistha Banerjee
Summary: HIV-1 integrates its genome into the host genome to establish infection, which requires safe transportation of subviral complexes and viral RNA across the nuclear membrane. This review summarizes the temporal and spatial events governing nucleocytoplasmic transport of HIV-1 factors and highlights the importance of identifying critical interactions for developing host-directed therapies.
Review
Biochemistry & Molecular Biology
Paola De Magistris
Summary: Nuclear export of mRNA through the NPC is crucial for protein translation in eukaryotic cells, with mRNAs forming ribonuclear particles with proteins for translocation. mRNPs undergo compositional and conformational changes during NPC transport, with multiple quality control steps to ensure proper export of mature transcripts to the cytoplasm. Opportunities for mechanistic understanding of nuclear export are mentioned in the review.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Merce Gomar-Alba, Vasilisa Pozharskaia, Bogdan Cichocki, Celia Schaal, Arun Kumar, Basile Jacquel, Gilles Charvin, J. Carlos Igual, Manuel Mendoza
Summary: Nuclear pore complexes (NPCs) mediate communication between the nucleus and the cytoplasm and regulate gene expression. Lysine acetyltransferase Esa1 acetylates the nuclear pore basket component Nup60, which recruits the mRNA export factor Sac3, to promote mRNA export. This mechanism also regulates cell entry into S phase and prevents premature commitment to a new cell division cycle in G1 daughter cells.
Article
Multidisciplinary Sciences
Willow Coyote-Maestas, David Nedrud, Antonio Suma, Yungui He, Kenneth A. Matreyek, Douglas M. Fowler, Vincenzo Carnevale, Chad L. Myers, Daniel Schmidt
Summary: The research conducted a large-scale, high-throughput biochemical assay to determine the compatibility of over 300,000 domain recombination variants of the inward rectifier K+ channel Kir2.1. They found that genomic and biophysical mechanisms acted together to favor gain of large, structured domains at protein termini during ion channel evolution.
NATURE COMMUNICATIONS
(2021)
Article
Immunology
Xiao Man Wu, Hong Fang, Jie Zhang, Yong Hong Bi, Ming Xian Chang
Summary: The study demonstrates the role of piscine H2A in negative regulation of the RLR signaling pathway and host immune response against SVCV infection. SVCV infection alters the subcellular localization of H2A from the nucleus to the cytoplasm, which in turn impairs the expression of genes involved in the RLR signaling pathway.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Sarah Kube, Nils Hersch, Elena Naumovska, Thomas Gensch, Johnny Hendriks, Arne Franzen, Lisa Landyogt, Jan-Peter Siebrasse, Ulrich Kubitscheck, Bernd Hoffmann, Rudolf Merkel, Agnes Csiszar
Review
Cell Biology
Ulrich Kubitscheck, Jan-Peter Siebrasse
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2017)
Article
Biochemical Research Methods
Lisa Landvogt, Jan Andreas Ruland, Christian Montellese, Jan Peter Siebrasse, Ulrike Kutay, Ulrich Kubitscheck
Article
Neurosciences
Jana Buergers, Irina Pavlova, Juan E. Rodriguez-Gatica, Christian Henneberger, Marc Oeller, Jan A. Ruland, Jan P. Siebrasse, Ulrich Kubitscheck, Martin K. Schwarz
Article
Neurosciences
Bhuvaneswari Nagarajan, Alexander Harder, Anna Japp, Felix Haeberlein, Enrico Mingardo, Henning Kleinert, Oeznur Yilmaz, Angelika Zoons, Birgit Rau, Andrea Christ, Ulrich Kubitscheck, Britta Eiberger, Roger Sandhoff, Matthias Eckhardt, Dieter Hartmann, Benjamin Odermatt
Article
Optics
Anne Stockhausen, Jana Buergers, Juan Eduardo Rodriguez-Gatica, Jens Schweihoff, Rudolf Merkel, Jens Markus Prigge, Martin Karl Schwarz, Ulrich Kubitscheck
Article
Multidisciplinary Sciences
Fabian Grein, Anna Mueller, Katharina M. Scherer, Xinliang Liu, Kevin C. Ludwig, Anna Kloeckner, Manuel Strach, Hans-Georg Sahl, Ulrich Kubitscheck, Tanja Schneider
NATURE COMMUNICATIONS
(2020)
Article
Urology & Nephrology
Annika Moller-Kerutt, Juan E. Rodriguez-Gatica, Karin Wacker, Rohan Bhatia, Jan-Peter Siebrasse, Nanda Boon, Veerle Van Marck, Peter Boor, Ulrich Kubitscheck, Jan Wijnholds, Hermann Pavenstadt, Thomas Weide
Summary: Mutations in the CRB2 gene can cause SRNS, and CRB2 is an essential component of podocyte slit diaphragm, interacting with Nephrin. Crb2(podKO) mice exhibit proteinuria, disordered foot processes, and increased renal injury and inflammatory markers.
JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY
(2021)
Review
Biochemistry & Molecular Biology
Martin K. Schwarz, Ulrich Kubitscheck
PROGRESS IN BIOPHYSICS & MOLECULAR BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Jan Andreas Ruland, Annika Marie Krueger, Kerstin Doerner, Rohan Bhatia, Sabine Wirths, Daniel Poetes, Ulrike Kutay, Jan Peter Siebrasse, Ulrich Kubitscheck
Summary: The study visualizes the export of single pre-60S particles through nuclear pore complexes using super-resolution confocal microscopy and single molecule tracking. They found that successful export events show maximum particle accumulation in the center of the pore, while unsuccessful export terminates within the nuclear basket. The export process has a single rate-limiting step and lasts approximately 24 milliseconds, with only about 1/3 of attempted export events being successful.
NATURE COMMUNICATIONS
(2021)
Article
Developmental Biology
Juan Eduardo Rodriguez-Gatica, Vira Iefremova, Liubov Sokhranyaeva, Si Wah Christina Au Yeung, Yannik Breitkreuz, Oliver Brustle, Martin Karl Schwarz, Ulrich Kubitscheck
Summary: Organoids, derived from stem cells, provide a new approach to model human development and disease. By using light-sheet fluorescence expansion microscopy, cellular and subcellular structural details of organoids can be observed in three dimensions, allowing for qualitative and quantitative assessment in developmental and disease-related studies.
Article
Anatomy & Morphology
Alexander Harder, Bhuvaneswari Nagarajan, Benjamin Odermatt, Ulrich Kubitscheck
Summary: Light sheet fluorescence microscopy (LSFM) is an essential tool in developmental biology for improving image contrast. This study presents an automatic synchronization method between excitation laser and detector, which does not require additional hardware components. A specific noise-tolerant focus metric was developed for calculating relative displacement, and an image analysis approach was used to determine laser tilt.
MICROSCOPY RESEARCH AND TECHNIQUE
(2023)
Article
Optics
Anne Stockhausen, Juan Eduardo Rodriguez-Gatica, Jens Schweihoff, Martin Karl Schwarz, Ulrich Kubitscheck
Summary: Traditional light sheet microscopy has a trade-off between the width of the light sheet and the usable field of view. To overcome this, low-diverging Airy beams have been introduced, but they suffer from side lobes that degrade image contrast. In this study, an Airy beam light sheet microscope was constructed, and a deep learning image deconvolution method was developed to remove the effects of side lobes. The combination of Airy beam light sheet microscopy and deep learning deconvolution allows for rapid and high-quality imaging of large volumes.
Article
Multidisciplinary Sciences
Jan -Samuel Puls, Dominik Brajtenbach, Tanja Schneider, Ulrich Kubitscheck, Fabian Grein
Summary: Bacterial cell wall biosynthesis is an important target of antibiotics, and its coordination with cell division is crucial. However, the role of peptidoglycan synthesis in cell division and the impact of antibiotics on this coordination are not fully understood. By visualizing key proteins in Staphylococcus aureus, we found that antibiotics targeting peptidoglycan synthesis can arrest cell division within minutes. Our work identifies cell division as a key target of these antibiotics and shows that peptidoglycan synthesis is essential for septum constriction throughout cell division of S. aureus.