Fusion-protein-assisted protein crystallization

Synthetic fusion protein design and applications

(2015) Kai Yu et al.   BIOTECHNOLOGY ADVANCES

A linker strategy for the production and crystallization of Toll/interleukin-1 receptor/resistance protein domain complexes

(2015) Simon J. Williams et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Positional effects of fusion partners on the yield and solubility of MBP fusion proteins

(2015) Sreejith Raran-Kurussi et al.   PROTEIN EXPRESSION AND PURIFICATION

Structure and function of Toll/interleukin-1 receptor/resistance protein (TIR) domains

(2014) Thomas Ve et al.   APOPTOSIS

New molecular engineering approaches for crystallographic studies of large RNAs

(2014) Jinwei Zhang et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Crystal structure of the essential Mycobacterium tuberculosis phosphopantetheinyl transferase PptT, solved as a fusion protein with maltose binding protein

(2014) James Jung et al.   JOURNAL OF STRUCTURAL BIOLOGY

Molecular control of δ-opioid receptor signalling

(2014) Gustavo Fenalti et al.   NATURE

The 2.1 Å Resolution Structure of Cyanopindolol-Bound β1-Adrenoceptor Identifies an Intramembrane Na+ Ion that Stabilises the Ligand-Free Receptor

(2014) Jennifer L. Miller-Gallacher et al.   PLoS One

Structural Basis for Assembly and Function of a Heterodimeric Plant Immune Receptor

(2014) S. J. Williams et al.   SCIENCE

Modified T4 Lysozyme Fusion Proteins Facilitate G Protein-Coupled Receptor Crystallogenesis

(2014) Thor Seneca Thorsen et al.   STRUCTURE

In situproteolysis, crystallization and preliminary X-ray diffraction analysis of a VHH that binds listeria internalin B

(2014) Ian Huh et al.   Acta Crystallographica Section F-Structural Biology Communications

Split green fluorescent protein as a modular binding partner for protein crystallization

(2013) Hau B. Nguyen et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

Structure of the caspase-recruitment domain from a zebrafish guanylate-binding protein

(2013) Tengchuan Jin et al.   Acta Crystallographica Section F-Structural Biology and Crystallization Communications

Bax Crystal Structures Reveal How BH3 Domains Activate Bax and Nucleate Its Oligomerization to Induce Apoptosis

(2013) Peter E. Czabotar et al.   CELL

Structure-function analyses of the human SIX1–EYA2 complex reveal insights into metastasis and BOR syndrome

(2013) Aaron N Patrick et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

To fuse or not to fuse: What is your purpose?

(2013) Mark R. Bell et al.   PROTEIN SCIENCE

Hexamers of the Type II Secretion ATPase GspE from Vibrio cholerae with Increased ATPase Activity

(2013) Connie Lu et al.   STRUCTURE

Explorations of linked editosome domains leading to the discovery of motifs defining conserved pockets in editosome OB-folds

(2012) Young-Jun Park et al.   JOURNAL OF STRUCTURAL BIOLOGY

A Carrier Protein Strategy Yields the Structure of Dalbavancin

(2012) Nicoleta J. Economou et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

N-Terminal T4 Lysozyme Fusion Facilitates Crystallization of a G Protein Coupled Receptor

(2012) Yaozhong Zou et al.   PLoS One

Design of a binding scaffold based on variable lymphocyte receptors of jawless vertebrates by module engineering

(2012) S.-C. Lee et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Linkers in the structural biology of protein-protein interactions

(2012) Vishnu Priyanka Reddy Chichili et al.   PROTEIN SCIENCE

Structural Basis for Allosteric Regulation of GPCRs by Sodium Ions

(2012) W. Liu et al.   SCIENCE

Fusion Partner Toolchest for the Stabilization and Crystallization of G Protein-Coupled Receptors

(2012) Eugene Chun et al.   STRUCTURE

Structural mix-n-match reveals molecular secrets of platelets

(2011) B. Kobe   BLOOD

Quaternary organization of GPIb-IX complex and insights into Bernard-Soulier syndrome revealed by the structures of GPIb  and a GPIb /GPIX chimera

(2011) P. A. McEwan et al.   BLOOD

Application of hybrid LRR technique to protein crystallization

(2011) Mi-Sun Jin et al.   BMB Reports

Crystallization of small proteins assisted by green fluorescent protein

(2010) Nobuhiro Suzuki et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

Application of protein engineering to enhance crystallizability and improve crystal properties

(2010) Zygmunt S. Derewenda   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

The structure of the dust mite allergen Der p 7 reveals similarities to innate immune proteins

(2010) Geoffrey A. Mueller et al.   JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY

Probing Native Protein Structures by Chemical Cross-linking, Mass Spectrometry, and Bioinformatics

(2010) Alexander Leitner et al.   MOLECULAR & CELLULAR PROTEOMICS

X-Ray Structures of the Hexameric Building Block of the HIV Capsid

(2009) Owen Pornillos et al.   CELL

Protein acrobatics in pairs—dimerization via domain swapping

(2009) Angela M Gronenborn   CURRENT OPINION IN STRUCTURAL BIOLOGY

Engineering of recombinant crystallization chaperones

(2009) Shohei Koide   CURRENT OPINION IN STRUCTURAL BIOLOGY

Structure of West Nile Virus NS3 Protease: Ligand Stabilization of the Catalytic Conformation

(2008) Gautier Robin et al.   JOURNAL OF MOLECULAR BIOLOGY

Protein production and purification

(2008) Susanne Gräslund et al.   NATURE METHODS

Redirecting the substrate specificity of heparan sulfate 2-O-sulfotransferase by structurally guided mutagenesis

(2008) H. N. Bethea et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Construction and characterization of a fully active PXR/SRC-1 tethered protein with increased stability

(2008) W. Wang et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Structure of a signal transduction regulator, RACK1, fromArabidopsis thaliana

(2008) Hemayet Ullah et al.   PROTEIN SCIENCE

The complex structure of calmodulin bound to a calcineurin peptide

(2008) Qilu Ye et al.   PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS